Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina

Autores
Spedaletti, Yamila Andrea; Aparicio Gonzalez, Monica Alicia; Mercado Cardenas, Guadalupe Eugenia; Rodriguero, Marcela Silvina; Taboada, Gisel María; Aban, Carla Luciana; Sühring, Silvia Susana; Vizgarra, Oscar Nicéforo; Galvan, Marta Zulema
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Common bean web blight (WB), caused by the fungus Rhizoctonia solani (teleomorph Thanatephorus cucumeris), is among the endemic fungal diseases of major impact in north‐western Argentina (NWA). This study aimed to analyse the genetic and pathogenic diversity of R. solani in Salta, NWA, where 97 isolates were recovered from commercial bean cultivars and wild beans showing WB symptoms in a major bean production area. The isolates were characterized on the basis of specific primers, rDNA‐ITS sequences and morphological characteristics. All the isolates were identified as R. solani AG 2‐2WB, and they exhibited considerable intragroup variation. The phylogenetic tree generated with the ITS sequences confirmed the isolates identification. Aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. A great variability in virulence was observed among the isolates analysed. On the basis of the disease reaction on foliar tissues, the isolates were grouped into three virulence categories as follows: weakly virulent (30%), moderately virulent (38%) and highly virulent (32%). However, no correlation between virulence and geographical origin was detected. The information generated in this study provides initial data on the population variability of the WB pathogen in north‐western Argentina and represents a valuable contribution to regional breeding programmes aimed to obtain cultivars with durable resistance.
EEA Salta
Fil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Aparicio Gonzalez, Monica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Mercado Cardenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina.
Fil: Taboada, Gisel María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Aban, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Sühring, Silvia Susana. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Vizgarra, Oscar N. Estación Experimental Agroindustrial Obispo Colombres; Argentina
Fil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fuente
Journal of Phytopathology 164 (11-12) : 1054-1063 (December 2016)
Materia
Phaseolus vulgaris
Fríjol (phaseolus)
Rhizoctonia solani
Enfermedades de las Plantas
Patogenicidad
Genética
Podredumbre de la Raíz
Kidney Beans
Plant Diseases
Pathogenicity
Genetics
Root Rots
Poroto
Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3619
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3619
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of ArgentinaSpedaletti, Yamila AndreaAparicio Gonzalez, Monica AliciaMercado Cardenas, Guadalupe EugeniaRodriguero, Marcela SilvinaTaboada, Gisel MaríaAban, Carla LucianaSühring, Silvia SusanaVizgarra, Oscar NicéforoGalvan, Marta ZulemaPhaseolus vulgarisFríjol (phaseolus)Rhizoctonia solaniEnfermedades de las PlantasPatogenicidadGenéticaPodredumbre de la RaízKidney BeansPlant DiseasesPathogenicityGeneticsRoot RotsPorotoArgentinaCommon bean web blight (WB), caused by the fungus Rhizoctonia solani (teleomorph Thanatephorus cucumeris), is among the endemic fungal diseases of major impact in north‐western Argentina (NWA). This study aimed to analyse the genetic and pathogenic diversity of R. solani in Salta, NWA, where 97 isolates were recovered from commercial bean cultivars and wild beans showing WB symptoms in a major bean production area. The isolates were characterized on the basis of specific primers, rDNA‐ITS sequences and morphological characteristics. All the isolates were identified as R. solani AG 2‐2WB, and they exhibited considerable intragroup variation. The phylogenetic tree generated with the ITS sequences confirmed the isolates identification. Aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. A great variability in virulence was observed among the isolates analysed. On the basis of the disease reaction on foliar tissues, the isolates were grouped into three virulence categories as follows: weakly virulent (30%), moderately virulent (38%) and highly virulent (32%). However, no correlation between virulence and geographical origin was detected. The information generated in this study provides initial data on the population variability of the WB pathogen in north‐western Argentina and represents a valuable contribution to regional breeding programmes aimed to obtain cultivars with durable resistance.EEA SaltaFil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Aparicio Gonzalez, Monica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Mercado Cardenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina.Fil: Taboada, Gisel María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Aban, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Sühring, Silvia Susana. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Vizgarra, Oscar N. Estación Experimental Agroindustrial Obispo Colombres; ArgentinaFil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina2018-10-17T17:44:17Z2018-10-17T17:44:17Z2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3619https://onlinelibrary.wiley.com/doi/full/10.1111/jph.125260931-17851439-0434https://doi.org/10.1111/jph.12526Journal of Phytopathology 164 (11-12) : 1054-1063 (December 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:37Zoai:localhost:20.500.12123/3619instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:37.991INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina
title Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina
spellingShingle Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina
Spedaletti, Yamila Andrea
Phaseolus vulgaris
Fríjol (phaseolus)
Rhizoctonia solani
Enfermedades de las Plantas
Patogenicidad
Genética
Podredumbre de la Raíz
Kidney Beans
Plant Diseases
Pathogenicity
Genetics
Root Rots
Poroto
Argentina
title_short Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina
title_full Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina
title_fullStr Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina
title_full_unstemmed Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina
title_sort Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina
dc.creator.none.fl_str_mv Spedaletti, Yamila Andrea
Aparicio Gonzalez, Monica Alicia
Mercado Cardenas, Guadalupe Eugenia
Rodriguero, Marcela Silvina
Taboada, Gisel María
Aban, Carla Luciana
Sühring, Silvia Susana
Vizgarra, Oscar Nicéforo
Galvan, Marta Zulema
author Spedaletti, Yamila Andrea
author_facet Spedaletti, Yamila Andrea
Aparicio Gonzalez, Monica Alicia
Mercado Cardenas, Guadalupe Eugenia
Rodriguero, Marcela Silvina
Taboada, Gisel María
Aban, Carla Luciana
Sühring, Silvia Susana
Vizgarra, Oscar Nicéforo
Galvan, Marta Zulema
author_role author
author2 Aparicio Gonzalez, Monica Alicia
Mercado Cardenas, Guadalupe Eugenia
Rodriguero, Marcela Silvina
Taboada, Gisel María
Aban, Carla Luciana
Sühring, Silvia Susana
Vizgarra, Oscar Nicéforo
Galvan, Marta Zulema
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Phaseolus vulgaris
Fríjol (phaseolus)
Rhizoctonia solani
Enfermedades de las Plantas
Patogenicidad
Genética
Podredumbre de la Raíz
Kidney Beans
Plant Diseases
Pathogenicity
Genetics
Root Rots
Poroto
Argentina
topic Phaseolus vulgaris
Fríjol (phaseolus)
Rhizoctonia solani
Enfermedades de las Plantas
Patogenicidad
Genética
Podredumbre de la Raíz
Kidney Beans
Plant Diseases
Pathogenicity
Genetics
Root Rots
Poroto
Argentina
dc.description.none.fl_txt_mv Common bean web blight (WB), caused by the fungus Rhizoctonia solani (teleomorph Thanatephorus cucumeris), is among the endemic fungal diseases of major impact in north‐western Argentina (NWA). This study aimed to analyse the genetic and pathogenic diversity of R. solani in Salta, NWA, where 97 isolates were recovered from commercial bean cultivars and wild beans showing WB symptoms in a major bean production area. The isolates were characterized on the basis of specific primers, rDNA‐ITS sequences and morphological characteristics. All the isolates were identified as R. solani AG 2‐2WB, and they exhibited considerable intragroup variation. The phylogenetic tree generated with the ITS sequences confirmed the isolates identification. Aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. A great variability in virulence was observed among the isolates analysed. On the basis of the disease reaction on foliar tissues, the isolates were grouped into three virulence categories as follows: weakly virulent (30%), moderately virulent (38%) and highly virulent (32%). However, no correlation between virulence and geographical origin was detected. The information generated in this study provides initial data on the population variability of the WB pathogen in north‐western Argentina and represents a valuable contribution to regional breeding programmes aimed to obtain cultivars with durable resistance.
EEA Salta
Fil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Aparicio Gonzalez, Monica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Mercado Cardenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina.
Fil: Taboada, Gisel María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Aban, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Sühring, Silvia Susana. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Vizgarra, Oscar N. Estación Experimental Agroindustrial Obispo Colombres; Argentina
Fil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
description Common bean web blight (WB), caused by the fungus Rhizoctonia solani (teleomorph Thanatephorus cucumeris), is among the endemic fungal diseases of major impact in north‐western Argentina (NWA). This study aimed to analyse the genetic and pathogenic diversity of R. solani in Salta, NWA, where 97 isolates were recovered from commercial bean cultivars and wild beans showing WB symptoms in a major bean production area. The isolates were characterized on the basis of specific primers, rDNA‐ITS sequences and morphological characteristics. All the isolates were identified as R. solani AG 2‐2WB, and they exhibited considerable intragroup variation. The phylogenetic tree generated with the ITS sequences confirmed the isolates identification. Aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. A great variability in virulence was observed among the isolates analysed. On the basis of the disease reaction on foliar tissues, the isolates were grouped into three virulence categories as follows: weakly virulent (30%), moderately virulent (38%) and highly virulent (32%). However, no correlation between virulence and geographical origin was detected. The information generated in this study provides initial data on the population variability of the WB pathogen in north‐western Argentina and represents a valuable contribution to regional breeding programmes aimed to obtain cultivars with durable resistance.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
2018-10-17T17:44:17Z
2018-10-17T17:44:17Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3619
https://onlinelibrary.wiley.com/doi/full/10.1111/jph.12526
0931-1785
1439-0434
https://doi.org/10.1111/jph.12526
url http://hdl.handle.net/20.500.12123/3619
https://onlinelibrary.wiley.com/doi/full/10.1111/jph.12526
https://doi.org/10.1111/jph.12526
identifier_str_mv 0931-1785
1439-0434
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of Phytopathology 164 (11-12) : 1054-1063 (December 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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