Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina

Autores
Spedaletti, Yamila Andrea; Mercado Cardenas, Guadalupe Eugenia; Taboada, Gisel María; Aban, Carla Luciana; Aparicio Gonzalez, Monica Alicia; Rodriguero, Marcela Silvina; Vizgarra, Oscar Nicéforo; Sühring, Silvia Susana; Galindez, Guadalupe; Galvan, Marta Zulema
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this study was to perform the molecular and pathogenic characterization of Rhizoctonia solani in bean seed and soil samples from fields with root rot symptoms in northwestern Argentina. Fifty-two Rhizoctonia spp. isolates were collected in ten naturally infested bean fields and characterized by morphological traits, DNA sequencing, and pathogenicity. The mycelium color, the pattern of sclerotia formation, and the number of nuclei per hyphal cell were determined for each isolate. According to the variability in the rDNA-ITS region, isolates were identified as R. solani (85%), Waitea circinata var. zeae (Rhizoctonia zeae) (2%) and Rhizoctonia spp. (13%). Most isolates of R. solani (92%) were found to belong to the anastomosis group (AG) AG 4, including seven AG 4 HG-I and nine AG 4 HG-III isolates. AGs obtained from soil samples were more variable than those obtained from seed samples. Molecular identification of the isolates was in agreement with their morphological characterization. In addition, aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. Four virulence categories were defined according to the disease reaction on root and foliar tissues, which showed great variability in virulence among the isolates. Our results suggest that both seed and soil-borne inoculum may play a significant role in pathogen dispersal in the region. This is the first study on Rhizoctonia species and AGs in bean seed and soil in this region and it may contribute towards an efficient control strategy for bean diseases caused by Rhizoctonia species.
EEA Salta
Fil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Mercado Cardenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Taboada, Gisel María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Aban, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Aparicio Gonzalez, Monica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina.
Fil: Vizgarra, Oscar N. Estación Experimental Agroindustrial Obispo Colombres; Argentina
Fil: Sühring, Silvia Susana. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Galindez, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales.
Fil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fuente
Australian Journal of Crop Science 11 (8) : 952-959 (August 2017)
Materia
Rhizoctonia solani
Phaseolus vulgaris
Fríjol (phaseolus)
Enfermedades de las Plantas
Patogenicidad
Podredumbre de la Raíz
Kidney Beans
Plant Diseases
Pathogenicity
Root Rots
Soil
Suelo
Argentina
Poroto
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3609
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3609
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of ArgentinaSpedaletti, Yamila AndreaMercado Cardenas, Guadalupe EugeniaTaboada, Gisel MaríaAban, Carla LucianaAparicio Gonzalez, Monica AliciaRodriguero, Marcela SilvinaVizgarra, Oscar NicéforoSühring, Silvia SusanaGalindez, GuadalupeGalvan, Marta ZulemaRhizoctonia solaniPhaseolus vulgarisFríjol (phaseolus)Enfermedades de las PlantasPatogenicidadPodredumbre de la RaízKidney BeansPlant DiseasesPathogenicityRoot RotsSoilSueloArgentinaPorotoThe objective of this study was to perform the molecular and pathogenic characterization of Rhizoctonia solani in bean seed and soil samples from fields with root rot symptoms in northwestern Argentina. Fifty-two Rhizoctonia spp. isolates were collected in ten naturally infested bean fields and characterized by morphological traits, DNA sequencing, and pathogenicity. The mycelium color, the pattern of sclerotia formation, and the number of nuclei per hyphal cell were determined for each isolate. According to the variability in the rDNA-ITS region, isolates were identified as R. solani (85%), Waitea circinata var. zeae (Rhizoctonia zeae) (2%) and Rhizoctonia spp. (13%). Most isolates of R. solani (92%) were found to belong to the anastomosis group (AG) AG 4, including seven AG 4 HG-I and nine AG 4 HG-III isolates. AGs obtained from soil samples were more variable than those obtained from seed samples. Molecular identification of the isolates was in agreement with their morphological characterization. In addition, aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. Four virulence categories were defined according to the disease reaction on root and foliar tissues, which showed great variability in virulence among the isolates. Our results suggest that both seed and soil-borne inoculum may play a significant role in pathogen dispersal in the region. This is the first study on Rhizoctonia species and AGs in bean seed and soil in this region and it may contribute towards an efficient control strategy for bean diseases caused by Rhizoctonia species.EEA SaltaFil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Mercado Cardenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Taboada, Gisel María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Aban, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Aparicio Gonzalez, Monica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina.Fil: Vizgarra, Oscar N. Estación Experimental Agroindustrial Obispo Colombres; ArgentinaFil: Sühring, Silvia Susana. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Galindez, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales.Fil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina2018-10-17T13:29:46Z2018-10-17T13:29:46Z2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/36091835-26931835-2707https://doi.org/10.21475/ajcs.17.11.08.pne432Australian Journal of Crop Science 11 (8) : 952-959 (August 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:37Zoai:localhost:20.500.12123/3609instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:37.96INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina
title Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina
spellingShingle Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina
Spedaletti, Yamila Andrea
Rhizoctonia solani
Phaseolus vulgaris
Fríjol (phaseolus)
Enfermedades de las Plantas
Patogenicidad
Podredumbre de la Raíz
Kidney Beans
Plant Diseases
Pathogenicity
Root Rots
Soil
Suelo
Argentina
Poroto
title_short Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina
title_full Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina
title_fullStr Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina
title_full_unstemmed Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina
title_sort Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina
dc.creator.none.fl_str_mv Spedaletti, Yamila Andrea
Mercado Cardenas, Guadalupe Eugenia
Taboada, Gisel María
Aban, Carla Luciana
Aparicio Gonzalez, Monica Alicia
Rodriguero, Marcela Silvina
Vizgarra, Oscar Nicéforo
Sühring, Silvia Susana
Galindez, Guadalupe
Galvan, Marta Zulema
author Spedaletti, Yamila Andrea
author_facet Spedaletti, Yamila Andrea
Mercado Cardenas, Guadalupe Eugenia
Taboada, Gisel María
Aban, Carla Luciana
Aparicio Gonzalez, Monica Alicia
Rodriguero, Marcela Silvina
Vizgarra, Oscar Nicéforo
Sühring, Silvia Susana
Galindez, Guadalupe
Galvan, Marta Zulema
author_role author
author2 Mercado Cardenas, Guadalupe Eugenia
Taboada, Gisel María
Aban, Carla Luciana
Aparicio Gonzalez, Monica Alicia
Rodriguero, Marcela Silvina
Vizgarra, Oscar Nicéforo
Sühring, Silvia Susana
Galindez, Guadalupe
Galvan, Marta Zulema
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Rhizoctonia solani
Phaseolus vulgaris
Fríjol (phaseolus)
Enfermedades de las Plantas
Patogenicidad
Podredumbre de la Raíz
Kidney Beans
Plant Diseases
Pathogenicity
Root Rots
Soil
Suelo
Argentina
Poroto
topic Rhizoctonia solani
Phaseolus vulgaris
Fríjol (phaseolus)
Enfermedades de las Plantas
Patogenicidad
Podredumbre de la Raíz
Kidney Beans
Plant Diseases
Pathogenicity
Root Rots
Soil
Suelo
Argentina
Poroto
dc.description.none.fl_txt_mv The objective of this study was to perform the molecular and pathogenic characterization of Rhizoctonia solani in bean seed and soil samples from fields with root rot symptoms in northwestern Argentina. Fifty-two Rhizoctonia spp. isolates were collected in ten naturally infested bean fields and characterized by morphological traits, DNA sequencing, and pathogenicity. The mycelium color, the pattern of sclerotia formation, and the number of nuclei per hyphal cell were determined for each isolate. According to the variability in the rDNA-ITS region, isolates were identified as R. solani (85%), Waitea circinata var. zeae (Rhizoctonia zeae) (2%) and Rhizoctonia spp. (13%). Most isolates of R. solani (92%) were found to belong to the anastomosis group (AG) AG 4, including seven AG 4 HG-I and nine AG 4 HG-III isolates. AGs obtained from soil samples were more variable than those obtained from seed samples. Molecular identification of the isolates was in agreement with their morphological characterization. In addition, aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. Four virulence categories were defined according to the disease reaction on root and foliar tissues, which showed great variability in virulence among the isolates. Our results suggest that both seed and soil-borne inoculum may play a significant role in pathogen dispersal in the region. This is the first study on Rhizoctonia species and AGs in bean seed and soil in this region and it may contribute towards an efficient control strategy for bean diseases caused by Rhizoctonia species.
EEA Salta
Fil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Mercado Cardenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Taboada, Gisel María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Aban, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Aparicio Gonzalez, Monica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina.
Fil: Vizgarra, Oscar N. Estación Experimental Agroindustrial Obispo Colombres; Argentina
Fil: Sühring, Silvia Susana. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Galindez, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales.
Fil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
description The objective of this study was to perform the molecular and pathogenic characterization of Rhizoctonia solani in bean seed and soil samples from fields with root rot symptoms in northwestern Argentina. Fifty-two Rhizoctonia spp. isolates were collected in ten naturally infested bean fields and characterized by morphological traits, DNA sequencing, and pathogenicity. The mycelium color, the pattern of sclerotia formation, and the number of nuclei per hyphal cell were determined for each isolate. According to the variability in the rDNA-ITS region, isolates were identified as R. solani (85%), Waitea circinata var. zeae (Rhizoctonia zeae) (2%) and Rhizoctonia spp. (13%). Most isolates of R. solani (92%) were found to belong to the anastomosis group (AG) AG 4, including seven AG 4 HG-I and nine AG 4 HG-III isolates. AGs obtained from soil samples were more variable than those obtained from seed samples. Molecular identification of the isolates was in agreement with their morphological characterization. In addition, aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. Four virulence categories were defined according to the disease reaction on root and foliar tissues, which showed great variability in virulence among the isolates. Our results suggest that both seed and soil-borne inoculum may play a significant role in pathogen dispersal in the region. This is the first study on Rhizoctonia species and AGs in bean seed and soil in this region and it may contribute towards an efficient control strategy for bean diseases caused by Rhizoctonia species.
publishDate 2017
dc.date.none.fl_str_mv 2017-08
2018-10-17T13:29:46Z
2018-10-17T13:29:46Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3609
1835-2693
1835-2707
https://doi.org/10.21475/ajcs.17.11.08.pne432
url http://hdl.handle.net/20.500.12123/3609
https://doi.org/10.21475/ajcs.17.11.08.pne432
identifier_str_mv 1835-2693
1835-2707
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Australian Journal of Crop Science 11 (8) : 952-959 (August 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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