Decrease in the incidence of charcoal root rot in common bean (Phaseolus vulgaris L.) by Bacillus amyloliquefaciens B14, a strain with PGPR properties

Autores
Sabate, Daniela Constanza; Perez Brandan, Carolina; Petroselli, Gabriela; Erra Balsells, Rosa; Audisio, Marcela Carina
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this study was to isolate strains of the genus Bacillus from different productive soils of the province of Salta, Argentina, which have growth promoting properties in common bean (Phaseolus vulgaris L.) and have the ability to inhibit different phytopathogenic fungi, primarily Macrophomina phaseolina. Among the 105 strains of bacilli checked, Bacillus sp. B14 was selected for having the greatest in vitro inhibitory effect against Sclerotium rolfsii, Sclerotinia sclerotiurum, Rhizoctonia solani, Fusarium solani and Macrophomina phaseolina, recording fungal inhibition values that varied between 60 and 80%. In addition, B14 produced auxins in a concentration of 10.10 mg/ml, and qualitatively synthesizes siderophores. Based on 16S rRNA gene sequencing, the strain was characterized as B. amyloliquefaciens. Data from greenhouse experiments showed that the black common bean cv. Nag 12 seeds inoculated with B14, had increased germination of 10%, as well as an increase in root length of 2 cm and in shoot length of 6 cm compared with the non-inoculated control seeds. When the phytosanitary state of the B14 inoculated seeds was analyzed, no growth of bacteria, or phytopathogenic fungi and contaminants was observed, while in the non-inoculated seeds, bacteria was found in 46% of seeds, in addition to other phytopathogenic fungi. B. amyloliquefaciens B14 reduced the incidence of M. phaseolina by 62% in the inoculated black bean cv. Nag 12 seeds. Furthermore, using MALDI-MS it was determined that the bacteria synthesized different lipopeptides in the presence of M. phaseolina, such as surfactin, iturin, fengycin, kurstatin and polymyxin, leading us to conclude that they are the main responsible for the antagonistic effect observed and that the nature of lipopeptides synthesized by B14 is influenced by target fungal strain.
EEA Salta
Fil: Sabate, Daniela Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química; Argentina. Universidad Nacional de Salta. Instituto de Investigación para la Industria Química; Argentina
Fil: Pérez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Petroselli, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química; Argentina. Universidad Nacional de Salta. Instituto de Investigación para la Industria Química; Argentina
Fuente
Biological Control 113 : 1-8 (October 2017)
Materia
Bacillus
Phaseolus vulgaris
Fríjol (phaseolus)
Carbón Vegetal
Podredumbre de la Raíz
Macrophomina phaseolina
Péptidos
Kidney Beans
Charcoal
Root Rots
Peptides
Bacillus amyloliquefaciens
Poroto
Lipopeptidos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3729
Acceder
id INTADig_4532d3b2e183722204b9b0d2bc757bc6
oai_identifier_str oai:localhost:20.500.12123/3729
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Decrease in the incidence of charcoal root rot in common bean (Phaseolus vulgaris L.) by Bacillus amyloliquefaciens B14, a strain with PGPR propertiesSabate, Daniela ConstanzaPerez Brandan, CarolinaPetroselli, GabrielaErra Balsells, RosaAudisio, Marcela CarinaBacillusPhaseolus vulgarisFríjol (phaseolus)Carbón VegetalPodredumbre de la RaízMacrophomina phaseolinaPéptidosKidney BeansCharcoalRoot RotsPeptidesBacillus amyloliquefaciensPorotoLipopeptidosThe objective of this study was to isolate strains of the genus Bacillus from different productive soils of the province of Salta, Argentina, which have growth promoting properties in common bean (Phaseolus vulgaris L.) and have the ability to inhibit different phytopathogenic fungi, primarily Macrophomina phaseolina. Among the 105 strains of bacilli checked, Bacillus sp. B14 was selected for having the greatest in vitro inhibitory effect against Sclerotium rolfsii, Sclerotinia sclerotiurum, Rhizoctonia solani, Fusarium solani and Macrophomina phaseolina, recording fungal inhibition values that varied between 60 and 80%. In addition, B14 produced auxins in a concentration of 10.10 mg/ml, and qualitatively synthesizes siderophores. Based on 16S rRNA gene sequencing, the strain was characterized as B. amyloliquefaciens. Data from greenhouse experiments showed that the black common bean cv. Nag 12 seeds inoculated with B14, had increased germination of 10%, as well as an increase in root length of 2 cm and in shoot length of 6 cm compared with the non-inoculated control seeds. When the phytosanitary state of the B14 inoculated seeds was analyzed, no growth of bacteria, or phytopathogenic fungi and contaminants was observed, while in the non-inoculated seeds, bacteria was found in 46% of seeds, in addition to other phytopathogenic fungi. B. amyloliquefaciens B14 reduced the incidence of M. phaseolina by 62% in the inoculated black bean cv. Nag 12 seeds. Furthermore, using MALDI-MS it was determined that the bacteria synthesized different lipopeptides in the presence of M. phaseolina, such as surfactin, iturin, fengycin, kurstatin and polymyxin, leading us to conclude that they are the main responsible for the antagonistic effect observed and that the nature of lipopeptides synthesized by B14 is influenced by target fungal strain.EEA SaltaFil: Sabate, Daniela Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química; Argentina. Universidad Nacional de Salta. Instituto de Investigación para la Industria Química; ArgentinaFil: Pérez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Petroselli, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; ArgentinaFil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; ArgentinaFil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química; Argentina. Universidad Nacional de Salta. Instituto de Investigación para la Industria Química; Argentina2018-10-29T13:34:33Z2018-10-29T13:34:33Z2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S1049964417301329http://hdl.handle.net/20.500.12123/37291049-9644https://doi.org/10.1016/j.biocontrol.2017.06.008Biological Control 113 : 1-8 (October 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:28Zoai:localhost:20.500.12123/3729instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:29.186INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Decrease in the incidence of charcoal root rot in common bean (Phaseolus vulgaris L.) by Bacillus amyloliquefaciens B14, a strain with PGPR properties
title Decrease in the incidence of charcoal root rot in common bean (Phaseolus vulgaris L.) by Bacillus amyloliquefaciens B14, a strain with PGPR properties
spellingShingle Decrease in the incidence of charcoal root rot in common bean (Phaseolus vulgaris L.) by Bacillus amyloliquefaciens B14, a strain with PGPR properties
Sabate, Daniela Constanza
Bacillus
Phaseolus vulgaris
Fríjol (phaseolus)
Carbón Vegetal
Podredumbre de la Raíz
Macrophomina phaseolina
Péptidos
Kidney Beans
Charcoal
Root Rots
Peptides
Bacillus amyloliquefaciens
Poroto
Lipopeptidos
title_short Decrease in the incidence of charcoal root rot in common bean (Phaseolus vulgaris L.) by Bacillus amyloliquefaciens B14, a strain with PGPR properties
title_full Decrease in the incidence of charcoal root rot in common bean (Phaseolus vulgaris L.) by Bacillus amyloliquefaciens B14, a strain with PGPR properties
title_fullStr Decrease in the incidence of charcoal root rot in common bean (Phaseolus vulgaris L.) by Bacillus amyloliquefaciens B14, a strain with PGPR properties
title_full_unstemmed Decrease in the incidence of charcoal root rot in common bean (Phaseolus vulgaris L.) by Bacillus amyloliquefaciens B14, a strain with PGPR properties
title_sort Decrease in the incidence of charcoal root rot in common bean (Phaseolus vulgaris L.) by Bacillus amyloliquefaciens B14, a strain with PGPR properties
dc.creator.none.fl_str_mv Sabate, Daniela Constanza
Perez Brandan, Carolina
Petroselli, Gabriela
Erra Balsells, Rosa
Audisio, Marcela Carina
author Sabate, Daniela Constanza
author_facet Sabate, Daniela Constanza
Perez Brandan, Carolina
Petroselli, Gabriela
Erra Balsells, Rosa
Audisio, Marcela Carina
author_role author
author2 Perez Brandan, Carolina
Petroselli, Gabriela
Erra Balsells, Rosa
Audisio, Marcela Carina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bacillus
Phaseolus vulgaris
Fríjol (phaseolus)
Carbón Vegetal
Podredumbre de la Raíz
Macrophomina phaseolina
Péptidos
Kidney Beans
Charcoal
Root Rots
Peptides
Bacillus amyloliquefaciens
Poroto
Lipopeptidos
topic Bacillus
Phaseolus vulgaris
Fríjol (phaseolus)
Carbón Vegetal
Podredumbre de la Raíz
Macrophomina phaseolina
Péptidos
Kidney Beans
Charcoal
Root Rots
Peptides
Bacillus amyloliquefaciens
Poroto
Lipopeptidos
dc.description.none.fl_txt_mv The objective of this study was to isolate strains of the genus Bacillus from different productive soils of the province of Salta, Argentina, which have growth promoting properties in common bean (Phaseolus vulgaris L.) and have the ability to inhibit different phytopathogenic fungi, primarily Macrophomina phaseolina. Among the 105 strains of bacilli checked, Bacillus sp. B14 was selected for having the greatest in vitro inhibitory effect against Sclerotium rolfsii, Sclerotinia sclerotiurum, Rhizoctonia solani, Fusarium solani and Macrophomina phaseolina, recording fungal inhibition values that varied between 60 and 80%. In addition, B14 produced auxins in a concentration of 10.10 mg/ml, and qualitatively synthesizes siderophores. Based on 16S rRNA gene sequencing, the strain was characterized as B. amyloliquefaciens. Data from greenhouse experiments showed that the black common bean cv. Nag 12 seeds inoculated with B14, had increased germination of 10%, as well as an increase in root length of 2 cm and in shoot length of 6 cm compared with the non-inoculated control seeds. When the phytosanitary state of the B14 inoculated seeds was analyzed, no growth of bacteria, or phytopathogenic fungi and contaminants was observed, while in the non-inoculated seeds, bacteria was found in 46% of seeds, in addition to other phytopathogenic fungi. B. amyloliquefaciens B14 reduced the incidence of M. phaseolina by 62% in the inoculated black bean cv. Nag 12 seeds. Furthermore, using MALDI-MS it was determined that the bacteria synthesized different lipopeptides in the presence of M. phaseolina, such as surfactin, iturin, fengycin, kurstatin and polymyxin, leading us to conclude that they are the main responsible for the antagonistic effect observed and that the nature of lipopeptides synthesized by B14 is influenced by target fungal strain.
EEA Salta
Fil: Sabate, Daniela Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química; Argentina. Universidad Nacional de Salta. Instituto de Investigación para la Industria Química; Argentina
Fil: Pérez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Petroselli, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química; Argentina. Universidad Nacional de Salta. Instituto de Investigación para la Industria Química; Argentina
description The objective of this study was to isolate strains of the genus Bacillus from different productive soils of the province of Salta, Argentina, which have growth promoting properties in common bean (Phaseolus vulgaris L.) and have the ability to inhibit different phytopathogenic fungi, primarily Macrophomina phaseolina. Among the 105 strains of bacilli checked, Bacillus sp. B14 was selected for having the greatest in vitro inhibitory effect against Sclerotium rolfsii, Sclerotinia sclerotiurum, Rhizoctonia solani, Fusarium solani and Macrophomina phaseolina, recording fungal inhibition values that varied between 60 and 80%. In addition, B14 produced auxins in a concentration of 10.10 mg/ml, and qualitatively synthesizes siderophores. Based on 16S rRNA gene sequencing, the strain was characterized as B. amyloliquefaciens. Data from greenhouse experiments showed that the black common bean cv. Nag 12 seeds inoculated with B14, had increased germination of 10%, as well as an increase in root length of 2 cm and in shoot length of 6 cm compared with the non-inoculated control seeds. When the phytosanitary state of the B14 inoculated seeds was analyzed, no growth of bacteria, or phytopathogenic fungi and contaminants was observed, while in the non-inoculated seeds, bacteria was found in 46% of seeds, in addition to other phytopathogenic fungi. B. amyloliquefaciens B14 reduced the incidence of M. phaseolina by 62% in the inoculated black bean cv. Nag 12 seeds. Furthermore, using MALDI-MS it was determined that the bacteria synthesized different lipopeptides in the presence of M. phaseolina, such as surfactin, iturin, fengycin, kurstatin and polymyxin, leading us to conclude that they are the main responsible for the antagonistic effect observed and that the nature of lipopeptides synthesized by B14 is influenced by target fungal strain.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
2018-10-29T13:34:33Z
2018-10-29T13:34:33Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S1049964417301329
http://hdl.handle.net/20.500.12123/3729
1049-9644
https://doi.org/10.1016/j.biocontrol.2017.06.008
url https://www.sciencedirect.com/science/article/pii/S1049964417301329
http://hdl.handle.net/20.500.12123/3729
https://doi.org/10.1016/j.biocontrol.2017.06.008
identifier_str_mv 1049-9644
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Biological Control 113 : 1-8 (October 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.558318

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