Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens

Autores
Torres, María Julia; Perez Brandan, Carolina; Sabate, Daniela Constanza; Petroselli, Gabriela; Erra Balsells, Rosa; Audisio, Marcela Carina
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacillus amyloliquefaciens PGPBacCA1 was studied regarding its aptitude to protect common bean seeds from their intrinsic pathogens. Also, the inhibition of different environmental phytopathogenic fungi was tested. Two cultivars of Phaseolus vulgaris L. were evaluated: cv. Nag (black bean) and cv. Alubia (white bean). Aspergillus spp., Penicillium spp. and Fusarium spp. constituted the natural fungal biota of both seeds, whereas white bean and black bean also exhibited Cladosporium spp. and Rhizopus spp., respectively. B. amyloliquefaciens PGPBacCA1 prevented the development of the endophytic fungi of black bean, while only Cladosporium spp. survived in the white variety. Growth chamber assays were carried out and bacilli cells were applied on seeds without affecting neither the vigor nor the germination potential of either type of bean. In addition, B. amyloliquefaciens PGPBacCA1, by dual cultures, was able to inhibit the development of the following phytopathogenic fungi: Sclerotium rolfsii (35%), Sclerotinia sclerotiorum (76.5%), Rhizoctonia solani (73%), Fusarium solani (56.5%), and Penicillium spp. (71.5%). The UV-MALDI TOF MS analysis showed that B. amyloliquefaciens PGPBacCA1 co-produces different homologues of the lipopeptides surfactin, iturin and fengycin in the presence of S. sclerotiorum and F. solani. These compounds were identified as the main responsible for the antagonistic effect. SEM analysis confirmed the antifungal effects of the lipopeptides, which also caused damage to chlamydospores and sclerotia of Fusarium and Sclerotinia, respectively. B. amyloliquefaciens PGPBacCA1 can thus be applied to these bean seeds varieties as a potential bioprotection agent.
EEA Salta
Fil: Torres, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Pérez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Sabate, Daniela Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química; Argentina. Universidad Nacional de Salta. Instituto de Investigación para la Industria Química; Argentina
Fil: Petroselli, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química; Argentina. Universidad Nacional de Salta. Instituto de Investigación para la Industria Química; Argentina
Fuente
Biological Control 105 : 93-99 (February 2017)
Materia
Bacillus
Phaseolus vulgaris
Fríjol (phaseolus)
Fusarium
Sclerotinia sclerotiorum
Péptidos
Kidney Beans
Peptides
Bacillus amyloliquefaciens
Poroto
Fusarium Solani
Lipopeptidos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3674
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3674
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogensTorres, María JuliaPerez Brandan, CarolinaSabate, Daniela ConstanzaPetroselli, GabrielaErra Balsells, RosaAudisio, Marcela CarinaBacillusPhaseolus vulgarisFríjol (phaseolus)FusariumSclerotinia sclerotiorumPéptidosKidney BeansPeptidesBacillus amyloliquefaciensPorotoFusarium SolaniLipopeptidosBacillus amyloliquefaciens PGPBacCA1 was studied regarding its aptitude to protect common bean seeds from their intrinsic pathogens. Also, the inhibition of different environmental phytopathogenic fungi was tested. Two cultivars of Phaseolus vulgaris L. were evaluated: cv. Nag (black bean) and cv. Alubia (white bean). Aspergillus spp., Penicillium spp. and Fusarium spp. constituted the natural fungal biota of both seeds, whereas white bean and black bean also exhibited Cladosporium spp. and Rhizopus spp., respectively. B. amyloliquefaciens PGPBacCA1 prevented the development of the endophytic fungi of black bean, while only Cladosporium spp. survived in the white variety. Growth chamber assays were carried out and bacilli cells were applied on seeds without affecting neither the vigor nor the germination potential of either type of bean. In addition, B. amyloliquefaciens PGPBacCA1, by dual cultures, was able to inhibit the development of the following phytopathogenic fungi: Sclerotium rolfsii (35%), Sclerotinia sclerotiorum (76.5%), Rhizoctonia solani (73%), Fusarium solani (56.5%), and Penicillium spp. (71.5%). The UV-MALDI TOF MS analysis showed that B. amyloliquefaciens PGPBacCA1 co-produces different homologues of the lipopeptides surfactin, iturin and fengycin in the presence of S. sclerotiorum and F. solani. These compounds were identified as the main responsible for the antagonistic effect. SEM analysis confirmed the antifungal effects of the lipopeptides, which also caused damage to chlamydospores and sclerotia of Fusarium and Sclerotinia, respectively. B. amyloliquefaciens PGPBacCA1 can thus be applied to these bean seeds varieties as a potential bioprotection agent.EEA SaltaFil: Torres, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Pérez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Sabate, Daniela Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química; Argentina. Universidad Nacional de Salta. Instituto de Investigación para la Industria Química; ArgentinaFil: Petroselli, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química; Argentina. Universidad Nacional de Salta. Instituto de Investigación para la Industria Química; Argentina2018-10-23T13:50:35Z2018-10-23T13:50:35Z2017-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S1049964416302419http://hdl.handle.net/20.500.12123/36741049-9644https://doi.org/10.1016/j.biocontrol.2016.12.001Biological Control 105 : 93-99 (February 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:37Zoai:localhost:20.500.12123/3674instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:38.277INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens
title Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens
spellingShingle Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens
Torres, María Julia
Bacillus
Phaseolus vulgaris
Fríjol (phaseolus)
Fusarium
Sclerotinia sclerotiorum
Péptidos
Kidney Beans
Peptides
Bacillus amyloliquefaciens
Poroto
Fusarium Solani
Lipopeptidos
title_short Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens
title_full Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens
title_fullStr Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens
title_full_unstemmed Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens
title_sort Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens
dc.creator.none.fl_str_mv Torres, María Julia
Perez Brandan, Carolina
Sabate, Daniela Constanza
Petroselli, Gabriela
Erra Balsells, Rosa
Audisio, Marcela Carina
author Torres, María Julia
author_facet Torres, María Julia
Perez Brandan, Carolina
Sabate, Daniela Constanza
Petroselli, Gabriela
Erra Balsells, Rosa
Audisio, Marcela Carina
author_role author
author2 Perez Brandan, Carolina
Sabate, Daniela Constanza
Petroselli, Gabriela
Erra Balsells, Rosa
Audisio, Marcela Carina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bacillus
Phaseolus vulgaris
Fríjol (phaseolus)
Fusarium
Sclerotinia sclerotiorum
Péptidos
Kidney Beans
Peptides
Bacillus amyloliquefaciens
Poroto
Fusarium Solani
Lipopeptidos
topic Bacillus
Phaseolus vulgaris
Fríjol (phaseolus)
Fusarium
Sclerotinia sclerotiorum
Péptidos
Kidney Beans
Peptides
Bacillus amyloliquefaciens
Poroto
Fusarium Solani
Lipopeptidos
dc.description.none.fl_txt_mv Bacillus amyloliquefaciens PGPBacCA1 was studied regarding its aptitude to protect common bean seeds from their intrinsic pathogens. Also, the inhibition of different environmental phytopathogenic fungi was tested. Two cultivars of Phaseolus vulgaris L. were evaluated: cv. Nag (black bean) and cv. Alubia (white bean). Aspergillus spp., Penicillium spp. and Fusarium spp. constituted the natural fungal biota of both seeds, whereas white bean and black bean also exhibited Cladosporium spp. and Rhizopus spp., respectively. B. amyloliquefaciens PGPBacCA1 prevented the development of the endophytic fungi of black bean, while only Cladosporium spp. survived in the white variety. Growth chamber assays were carried out and bacilli cells were applied on seeds without affecting neither the vigor nor the germination potential of either type of bean. In addition, B. amyloliquefaciens PGPBacCA1, by dual cultures, was able to inhibit the development of the following phytopathogenic fungi: Sclerotium rolfsii (35%), Sclerotinia sclerotiorum (76.5%), Rhizoctonia solani (73%), Fusarium solani (56.5%), and Penicillium spp. (71.5%). The UV-MALDI TOF MS analysis showed that B. amyloliquefaciens PGPBacCA1 co-produces different homologues of the lipopeptides surfactin, iturin and fengycin in the presence of S. sclerotiorum and F. solani. These compounds were identified as the main responsible for the antagonistic effect. SEM analysis confirmed the antifungal effects of the lipopeptides, which also caused damage to chlamydospores and sclerotia of Fusarium and Sclerotinia, respectively. B. amyloliquefaciens PGPBacCA1 can thus be applied to these bean seeds varieties as a potential bioprotection agent.
EEA Salta
Fil: Torres, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Pérez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Sabate, Daniela Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química; Argentina. Universidad Nacional de Salta. Instituto de Investigación para la Industria Química; Argentina
Fil: Petroselli, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química; Argentina. Universidad Nacional de Salta. Instituto de Investigación para la Industria Química; Argentina
description Bacillus amyloliquefaciens PGPBacCA1 was studied regarding its aptitude to protect common bean seeds from their intrinsic pathogens. Also, the inhibition of different environmental phytopathogenic fungi was tested. Two cultivars of Phaseolus vulgaris L. were evaluated: cv. Nag (black bean) and cv. Alubia (white bean). Aspergillus spp., Penicillium spp. and Fusarium spp. constituted the natural fungal biota of both seeds, whereas white bean and black bean also exhibited Cladosporium spp. and Rhizopus spp., respectively. B. amyloliquefaciens PGPBacCA1 prevented the development of the endophytic fungi of black bean, while only Cladosporium spp. survived in the white variety. Growth chamber assays were carried out and bacilli cells were applied on seeds without affecting neither the vigor nor the germination potential of either type of bean. In addition, B. amyloliquefaciens PGPBacCA1, by dual cultures, was able to inhibit the development of the following phytopathogenic fungi: Sclerotium rolfsii (35%), Sclerotinia sclerotiorum (76.5%), Rhizoctonia solani (73%), Fusarium solani (56.5%), and Penicillium spp. (71.5%). The UV-MALDI TOF MS analysis showed that B. amyloliquefaciens PGPBacCA1 co-produces different homologues of the lipopeptides surfactin, iturin and fengycin in the presence of S. sclerotiorum and F. solani. These compounds were identified as the main responsible for the antagonistic effect. SEM analysis confirmed the antifungal effects of the lipopeptides, which also caused damage to chlamydospores and sclerotia of Fusarium and Sclerotinia, respectively. B. amyloliquefaciens PGPBacCA1 can thus be applied to these bean seeds varieties as a potential bioprotection agent.
publishDate 2017
dc.date.none.fl_str_mv 2017-02
2018-10-23T13:50:35Z
2018-10-23T13:50:35Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S1049964416302419
http://hdl.handle.net/20.500.12123/3674
1049-9644
https://doi.org/10.1016/j.biocontrol.2016.12.001
url https://www.sciencedirect.com/science/article/pii/S1049964416302419
http://hdl.handle.net/20.500.12123/3674
https://doi.org/10.1016/j.biocontrol.2016.12.001
identifier_str_mv 1049-9644
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Biological Control 105 : 93-99 (February 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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