Isolation and characterization of a Bacillus thurigiensis strain with potential for epizootics in Plodia interpunctella

Autores
Guaraz, Agustin Bosio; Ortiz, Leila Melina; Nieves, Eliana; Salas, Augusto; Sauka, Diego Hernan
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Póster y resumen
Bacillus thuringiensis is a gram-positive, spore-forming bacterium renowned for its production of pesticidal proteins, which make it highly effective against a wide range of insect pests. These proteins, primarily Cry and Vip, specifically target the gut epithelium of susceptible insects, causing feeding cessation and eventual death. As a result, B. thuringiensis has become a cornerstone of biological pest control, particularly in integrated pest management programs. Although widely used, B. thuringiensis is generally considered an opportunistic entomopathogen due to the rarity of natural epizootics. However, certain strains have been linked to significant pest mortality in specific environments. This study focused on isolating and characterizing B. thuringiensis strain INTA L404-1, which was associated with high mortality in an artificially reared population of Plodia interpunctella (Lepidoptera: Pyralidae), a significant pest of stored grains. By investigating its genetic profile, the phenotypic characteristics of its crystals, and its insecticidal properties, we aim to evaluate its potential as an effective biocontrol agent.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Guaraz, Agustin Bosio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Ortiz, Leila Melina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Nieves, Eliana. Universidad Nacional de La Plata (UNLP). Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Nieves, Eliana. Comisión de Investigaciones Científicas (CIC). Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Nieves, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Salas, Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Salas, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fuente
4th International Electronic Conference on Agronomy, 2 al 5 de diciembre de 2024 (virtual)
Materia
Aislamiento
Caracterización
Cepas
Epizootia
Bacillus thuringiensis
Plodia interpunctella
Control Biológico
Isolation
Characterization
Strains
Epizootics
Biological Control
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Isolation and characterization of a Bacillus thurigiensis strain with potential for epizootics in Plodia interpunctellaGuaraz, Agustin BosioOrtiz, Leila MelinaNieves, ElianaSalas, AugustoSauka, Diego HernanAislamientoCaracterizaciónCepasEpizootiaBacillus thuringiensisPlodia interpunctellaControl BiológicoIsolationCharacterizationStrainsEpizooticsBiological ControlPóster y resumenBacillus thuringiensis is a gram-positive, spore-forming bacterium renowned for its production of pesticidal proteins, which make it highly effective against a wide range of insect pests. These proteins, primarily Cry and Vip, specifically target the gut epithelium of susceptible insects, causing feeding cessation and eventual death. As a result, B. thuringiensis has become a cornerstone of biological pest control, particularly in integrated pest management programs. Although widely used, B. thuringiensis is generally considered an opportunistic entomopathogen due to the rarity of natural epizootics. However, certain strains have been linked to significant pest mortality in specific environments. This study focused on isolating and characterizing B. thuringiensis strain INTA L404-1, which was associated with high mortality in an artificially reared population of Plodia interpunctella (Lepidoptera: Pyralidae), a significant pest of stored grains. By investigating its genetic profile, the phenotypic characteristics of its crystals, and its insecticidal properties, we aim to evaluate its potential as an effective biocontrol agent.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Guaraz, Agustin Bosio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Ortiz, Leila Melina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Nieves, Eliana. Universidad Nacional de La Plata (UNLP). Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Nieves, Eliana. Comisión de Investigaciones Científicas (CIC). Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Nieves, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Salas, Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Salas, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaMultidisciplinary Digital Publishing Institute (MDPI)2025-01-31T16:37:39Z2025-01-31T16:37:39Z2024-12-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/211164th International Electronic Conference on Agronomy, 2 al 5 de diciembre de 2024 (virtual)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:53Zoai:localhost:20.500.12123/21116instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:53.456INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Isolation and characterization of a Bacillus thurigiensis strain with potential for epizootics in Plodia interpunctella
title Isolation and characterization of a Bacillus thurigiensis strain with potential for epizootics in Plodia interpunctella
spellingShingle Isolation and characterization of a Bacillus thurigiensis strain with potential for epizootics in Plodia interpunctella
Guaraz, Agustin Bosio
Aislamiento
Caracterización
Cepas
Epizootia
Bacillus thuringiensis
Plodia interpunctella
Control Biológico
Isolation
Characterization
Strains
Epizootics
Biological Control
title_short Isolation and characterization of a Bacillus thurigiensis strain with potential for epizootics in Plodia interpunctella
title_full Isolation and characterization of a Bacillus thurigiensis strain with potential for epizootics in Plodia interpunctella
title_fullStr Isolation and characterization of a Bacillus thurigiensis strain with potential for epizootics in Plodia interpunctella
title_full_unstemmed Isolation and characterization of a Bacillus thurigiensis strain with potential for epizootics in Plodia interpunctella
title_sort Isolation and characterization of a Bacillus thurigiensis strain with potential for epizootics in Plodia interpunctella
dc.creator.none.fl_str_mv Guaraz, Agustin Bosio
Ortiz, Leila Melina
Nieves, Eliana
Salas, Augusto
Sauka, Diego Hernan
author Guaraz, Agustin Bosio
author_facet Guaraz, Agustin Bosio
Ortiz, Leila Melina
Nieves, Eliana
Salas, Augusto
Sauka, Diego Hernan
author_role author
author2 Ortiz, Leila Melina
Nieves, Eliana
Salas, Augusto
Sauka, Diego Hernan
author2_role author
author
author
author
dc.subject.none.fl_str_mv Aislamiento
Caracterización
Cepas
Epizootia
Bacillus thuringiensis
Plodia interpunctella
Control Biológico
Isolation
Characterization
Strains
Epizootics
Biological Control
topic Aislamiento
Caracterización
Cepas
Epizootia
Bacillus thuringiensis
Plodia interpunctella
Control Biológico
Isolation
Characterization
Strains
Epizootics
Biological Control
dc.description.none.fl_txt_mv Póster y resumen
Bacillus thuringiensis is a gram-positive, spore-forming bacterium renowned for its production of pesticidal proteins, which make it highly effective against a wide range of insect pests. These proteins, primarily Cry and Vip, specifically target the gut epithelium of susceptible insects, causing feeding cessation and eventual death. As a result, B. thuringiensis has become a cornerstone of biological pest control, particularly in integrated pest management programs. Although widely used, B. thuringiensis is generally considered an opportunistic entomopathogen due to the rarity of natural epizootics. However, certain strains have been linked to significant pest mortality in specific environments. This study focused on isolating and characterizing B. thuringiensis strain INTA L404-1, which was associated with high mortality in an artificially reared population of Plodia interpunctella (Lepidoptera: Pyralidae), a significant pest of stored grains. By investigating its genetic profile, the phenotypic characteristics of its crystals, and its insecticidal properties, we aim to evaluate its potential as an effective biocontrol agent.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Guaraz, Agustin Bosio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Ortiz, Leila Melina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Nieves, Eliana. Universidad Nacional de La Plata (UNLP). Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Nieves, Eliana. Comisión de Investigaciones Científicas (CIC). Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Nieves, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Salas, Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Salas, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
description Póster y resumen
publishDate 2024
dc.date.none.fl_str_mv 2024-12-05
2025-01-31T16:37:39Z
2025-01-31T16:37:39Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21116
url http://hdl.handle.net/20.500.12123/21116
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv 4th International Electronic Conference on Agronomy, 2 al 5 de diciembre de 2024 (virtual)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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