Protocol for the fast isolation and identification of insecticidal Bacillus thuringiensis strains from soil

Autores
Palma, Leopoldo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacillus thuringiensis (Bt) is an ubiquitous, Gram-positive and sporulating bacterium that synthesizes insecticidal proteins with specificity against a wide range of insects during sporulation (Cry and Cyt) and vegetative growth (Vip and Sip). These proteins have portrayed Bt as an environment-friendly alternative to chemical insecticides. However, the intensive use of this resource has promoted the evolution of insect resistance to some of the most used Bt strains to date. Continuous efforts are needed to obtain novel strains exhibiting new specificities in order to overcome insect resistance and maintaining the insecticidal potential of this bacterium. In this work, an experimental procedure for the fast isolation and identification of insecticidal Bt strains is described.
Fil: Palma, Leopoldo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Materia
BACILLUS THURINGIENSIS
INSECTICIDAL STRAINS
INSECTICIDAL TOXINS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/44693
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/44693
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Protocol for the fast isolation and identification of insecticidal Bacillus thuringiensis strains from soilPalma, LeopoldoBACILLUS THURINGIENSISINSECTICIDAL STRAINSINSECTICIDAL TOXINShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bacillus thuringiensis (Bt) is an ubiquitous, Gram-positive and sporulating bacterium that synthesizes insecticidal proteins with specificity against a wide range of insects during sporulation (Cry and Cyt) and vegetative growth (Vip and Sip). These proteins have portrayed Bt as an environment-friendly alternative to chemical insecticides. However, the intensive use of this resource has promoted the evolution of insect resistance to some of the most used Bt strains to date. Continuous efforts are needed to obtain novel strains exhibiting new specificities in order to overcome insect resistance and maintaining the insecticidal potential of this bacterium. In this work, an experimental procedure for the fast isolation and identification of insecticidal Bt strains is described.Fil: Palma, Leopoldo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaBiopublisher2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/44693Palma, Leopoldo; Protocol for the fast isolation and identification of insecticidal Bacillus thuringiensis strains from soil; Biopublisher; Bt Research; 6; 7; 10-2015; 1-31925-1939CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://biopublisher.ca/index.php/bt/article/view/2019/1870info:eu-repo/semantics/altIdentifier/doi/10.5376/bt.2015.06.0007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:09Zoai:ri.conicet.gov.ar:11336/44693instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:09.735CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protocol for the fast isolation and identification of insecticidal Bacillus thuringiensis strains from soil
title Protocol for the fast isolation and identification of insecticidal Bacillus thuringiensis strains from soil
spellingShingle Protocol for the fast isolation and identification of insecticidal Bacillus thuringiensis strains from soil
Palma, Leopoldo
BACILLUS THURINGIENSIS
INSECTICIDAL STRAINS
INSECTICIDAL TOXINS
title_short Protocol for the fast isolation and identification of insecticidal Bacillus thuringiensis strains from soil
title_full Protocol for the fast isolation and identification of insecticidal Bacillus thuringiensis strains from soil
title_fullStr Protocol for the fast isolation and identification of insecticidal Bacillus thuringiensis strains from soil
title_full_unstemmed Protocol for the fast isolation and identification of insecticidal Bacillus thuringiensis strains from soil
title_sort Protocol for the fast isolation and identification of insecticidal Bacillus thuringiensis strains from soil
dc.creator.none.fl_str_mv Palma, Leopoldo
author Palma, Leopoldo
author_facet Palma, Leopoldo
author_role author
dc.subject.none.fl_str_mv BACILLUS THURINGIENSIS
INSECTICIDAL STRAINS
INSECTICIDAL TOXINS
topic BACILLUS THURINGIENSIS
INSECTICIDAL STRAINS
INSECTICIDAL TOXINS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bacillus thuringiensis (Bt) is an ubiquitous, Gram-positive and sporulating bacterium that synthesizes insecticidal proteins with specificity against a wide range of insects during sporulation (Cry and Cyt) and vegetative growth (Vip and Sip). These proteins have portrayed Bt as an environment-friendly alternative to chemical insecticides. However, the intensive use of this resource has promoted the evolution of insect resistance to some of the most used Bt strains to date. Continuous efforts are needed to obtain novel strains exhibiting new specificities in order to overcome insect resistance and maintaining the insecticidal potential of this bacterium. In this work, an experimental procedure for the fast isolation and identification of insecticidal Bt strains is described.
Fil: Palma, Leopoldo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
description Bacillus thuringiensis (Bt) is an ubiquitous, Gram-positive and sporulating bacterium that synthesizes insecticidal proteins with specificity against a wide range of insects during sporulation (Cry and Cyt) and vegetative growth (Vip and Sip). These proteins have portrayed Bt as an environment-friendly alternative to chemical insecticides. However, the intensive use of this resource has promoted the evolution of insect resistance to some of the most used Bt strains to date. Continuous efforts are needed to obtain novel strains exhibiting new specificities in order to overcome insect resistance and maintaining the insecticidal potential of this bacterium. In this work, an experimental procedure for the fast isolation and identification of insecticidal Bt strains is described.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/44693
Palma, Leopoldo; Protocol for the fast isolation and identification of insecticidal Bacillus thuringiensis strains from soil; Biopublisher; Bt Research; 6; 7; 10-2015; 1-3
1925-1939
CONICET Digital
CONICET
url http://hdl.handle.net/11336/44693
identifier_str_mv Palma, Leopoldo; Protocol for the fast isolation and identification of insecticidal Bacillus thuringiensis strains from soil; Biopublisher; Bt Research; 6; 7; 10-2015; 1-3
1925-1939
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://biopublisher.ca/index.php/bt/article/view/2019/1870
info:eu-repo/semantics/altIdentifier/doi/10.5376/bt.2015.06.0007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Biopublisher
publisher.none.fl_str_mv Biopublisher
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269679034302464
score 13.13397

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