South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages

Autores
Ferreri, Lucas Matias; Carnaccini, Silvia; Olivera, Valeria Soledad; Pereda, Ariel Julian; Rajao, Daniela; Perez, Daniel R.
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction of influenza A viruses (FLUAV) into poultry from waterfowl is frequent, producing economic burden and increasing the probability of human infections. We have previously described the presence of FLUAV in wild birds in Argentina with unique evolutionary trajectories belonging to a South American lineage different from the North American and Eurasian lineages. Adaptability of this South American lineage FLUAV to poultry species is still poorly understood. In the present report, we evaluated the capacity of an H4N2 FLUAV from the South American lineage to adapt to chickens after low number of passages. We found that five mutations were acquired after five passages in 3-days-old chickens. These mutations produced a virus with better infectivity in ex vivo trachea explants but overall lower infection in lung explants. Infection of 3-week-old chickens persisted for a longer period and was detected in more tissues than the parental virus, suggesting adaptation of the H4N2 influenza A virus to chicken.
Instituto de Virología
Fil: Ferreri, Lucas. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
Fil: Ferreri, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Carnaccini, Silvia. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Pereda, Ariel Julian. Instituto Nacional de Tecnología Agropecuaria (INTA). Programa Nacional Salud Animal; Argentina
Fil: Rajao, Daniela. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
Fil: Perez, Daniel R. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
Fuente
Frontiers in Veterinary Science 10 : 1182550. (May 2023)
Materia
Pollo
Virus de la Influenza Aviar
Aves de Corral
Aves Acuáticas
Enfermedades de los Animales
Chickens
Avian Influenzavirus
Poultry
Waterfowl
Animal Diseases
South America
América del Sur
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/14704
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oai_identifier_str oai:localhost:20.500.12123/14704
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spelling South American H4N2 influenza A virus improved replication in chicken trachea after low number of passagesFerreri, Lucas MatiasCarnaccini, SilviaOlivera, Valeria SoledadPereda, Ariel JulianRajao, DanielaPerez, Daniel R.PolloVirus de la Influenza AviarAves de CorralAves AcuáticasEnfermedades de los AnimalesChickensAvian InfluenzavirusPoultryWaterfowlAnimal DiseasesSouth AmericaAmérica del SurIntroduction of influenza A viruses (FLUAV) into poultry from waterfowl is frequent, producing economic burden and increasing the probability of human infections. We have previously described the presence of FLUAV in wild birds in Argentina with unique evolutionary trajectories belonging to a South American lineage different from the North American and Eurasian lineages. Adaptability of this South American lineage FLUAV to poultry species is still poorly understood. In the present report, we evaluated the capacity of an H4N2 FLUAV from the South American lineage to adapt to chickens after low number of passages. We found that five mutations were acquired after five passages in 3-days-old chickens. These mutations produced a virus with better infectivity in ex vivo trachea explants but overall lower infection in lung explants. Infection of 3-week-old chickens persisted for a longer period and was detected in more tissues than the parental virus, suggesting adaptation of the H4N2 influenza A virus to chicken.Instituto de VirologíaFil: Ferreri, Lucas. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados UnidosFil: Ferreri, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Carnaccini, Silvia. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados UnidosFil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Pereda, Ariel Julian. Instituto Nacional de Tecnología Agropecuaria (INTA). Programa Nacional Salud Animal; ArgentinaFil: Rajao, Daniela. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados UnidosFil: Perez, Daniel R. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados UnidosFrontiers Media2023-07-05T18:32:31Z2023-07-05T18:32:31Z2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14704https://www.frontiersin.org/articles/10.3389/fvets.2023.1182550/full2297-1769https://doi.org/10.3389/fvets.2023.1182550Frontiers in Veterinary Science 10 : 1182550. (May 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.info:eu-repograntAgreement/INTA/PNSA-1115056/AR./Enfermedades infecciosas de las aves.South America .......... (continent) (World)1000002info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:00Zoai:localhost:20.500.12123/14704instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:00.702INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages
title South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages
spellingShingle South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages
Ferreri, Lucas Matias
Pollo
Virus de la Influenza Aviar
Aves de Corral
Aves Acuáticas
Enfermedades de los Animales
Chickens
Avian Influenzavirus
Poultry
Waterfowl
Animal Diseases
South America
América del Sur
title_short South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages
title_full South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages
title_fullStr South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages
title_full_unstemmed South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages
title_sort South American H4N2 influenza A virus improved replication in chicken trachea after low number of passages
dc.creator.none.fl_str_mv Ferreri, Lucas Matias
Carnaccini, Silvia
Olivera, Valeria Soledad
Pereda, Ariel Julian
Rajao, Daniela
Perez, Daniel R.
author Ferreri, Lucas Matias
author_facet Ferreri, Lucas Matias
Carnaccini, Silvia
Olivera, Valeria Soledad
Pereda, Ariel Julian
Rajao, Daniela
Perez, Daniel R.
author_role author
author2 Carnaccini, Silvia
Olivera, Valeria Soledad
Pereda, Ariel Julian
Rajao, Daniela
Perez, Daniel R.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Pollo
Virus de la Influenza Aviar
Aves de Corral
Aves Acuáticas
Enfermedades de los Animales
Chickens
Avian Influenzavirus
Poultry
Waterfowl
Animal Diseases
South America
América del Sur
topic Pollo
Virus de la Influenza Aviar
Aves de Corral
Aves Acuáticas
Enfermedades de los Animales
Chickens
Avian Influenzavirus
Poultry
Waterfowl
Animal Diseases
South America
América del Sur
dc.description.none.fl_txt_mv Introduction of influenza A viruses (FLUAV) into poultry from waterfowl is frequent, producing economic burden and increasing the probability of human infections. We have previously described the presence of FLUAV in wild birds in Argentina with unique evolutionary trajectories belonging to a South American lineage different from the North American and Eurasian lineages. Adaptability of this South American lineage FLUAV to poultry species is still poorly understood. In the present report, we evaluated the capacity of an H4N2 FLUAV from the South American lineage to adapt to chickens after low number of passages. We found that five mutations were acquired after five passages in 3-days-old chickens. These mutations produced a virus with better infectivity in ex vivo trachea explants but overall lower infection in lung explants. Infection of 3-week-old chickens persisted for a longer period and was detected in more tissues than the parental virus, suggesting adaptation of the H4N2 influenza A virus to chicken.
Instituto de Virología
Fil: Ferreri, Lucas. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
Fil: Ferreri, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Carnaccini, Silvia. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Pereda, Ariel Julian. Instituto Nacional de Tecnología Agropecuaria (INTA). Programa Nacional Salud Animal; Argentina
Fil: Rajao, Daniela. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
Fil: Perez, Daniel R. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
description Introduction of influenza A viruses (FLUAV) into poultry from waterfowl is frequent, producing economic burden and increasing the probability of human infections. We have previously described the presence of FLUAV in wild birds in Argentina with unique evolutionary trajectories belonging to a South American lineage different from the North American and Eurasian lineages. Adaptability of this South American lineage FLUAV to poultry species is still poorly understood. In the present report, we evaluated the capacity of an H4N2 FLUAV from the South American lineage to adapt to chickens after low number of passages. We found that five mutations were acquired after five passages in 3-days-old chickens. These mutations produced a virus with better infectivity in ex vivo trachea explants but overall lower infection in lung explants. Infection of 3-week-old chickens persisted for a longer period and was detected in more tissues than the parental virus, suggesting adaptation of the H4N2 influenza A virus to chicken.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-05T18:32:31Z
2023-07-05T18:32:31Z
2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14704
https://www.frontiersin.org/articles/10.3389/fvets.2023.1182550/full
2297-1769
https://doi.org/10.3389/fvets.2023.1182550
url http://hdl.handle.net/20.500.12123/14704
https://www.frontiersin.org/articles/10.3389/fvets.2023.1182550/full
https://doi.org/10.3389/fvets.2023.1182550
identifier_str_mv 2297-1769
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.
info:eu-repograntAgreement/INTA/PNSA-1115056/AR./Enfermedades infecciosas de las aves.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv South America .......... (continent) (World)
1000002
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Frontiers in Veterinary Science 10 : 1182550. (May 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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