Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina

Autores
Crociara, Clara Sonia; Valetti, Lucio; Bernanrdi Lima, Nelson; Iglesias, Juliana; Pastor, Silvina
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ascochyta blight is one of the most devastating foliar diseases of chickpea. The causal agent, Ascochyta rabiei, is a heterothallic ascomycete and its sexual reproduction depends on the proximate presence of both mating types, MAT1-1 and MAT1-2. Since its first detection in Argentina in 2011, very few studies have been carried out and information on local isolates remains unknown. In this work, 12 isolates were obtained from the northern, central and southern regions of Córdoba province, Argentina. First, isolates were tentatively identified as A. rabiei based on their colony and conidial characteristics. Identity was confirmed by phylogenetic analysis of the ITS, β-tubulin and D1/D2 loci. Morphology, cultural characteristics and pathogenic variability of the isolates were compared. Likewise, 27 crosses were made to demonstrate possible sexual reproduction between the strains. The isolates showed differences in morphology (shape, texture, conidial size, colony colour, growth rate) and aggressiveness from a susceptible cultivar. All crosses were fertile and produced pseudothecia, asci and ascospores. This work is the first in Latin America to characterize isolates of A. rabiei morphologically and molecularly, examine their pathogenic variability and demonstrate the sexual reproduction between isolates carrying different mating-type idiomorphs. New levels of aggressiveness in A. rabiei or adaptive changes like resistance to fungicides or breakdown of host resistance are a distinct possibility in the future.
EEA Pergamino
Fil: Crociara, Clara Sonia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Crociara, Clara Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Bernardi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bernardi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Iglesias, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Departamento de Maíz; Argentina
Fil: Iglesias, Juliana. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Agrarias, Naturales y Ambientales; Argentina
Fil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Pastor, Silvina Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fuente
Journal of Phytopathology 170 (1) : 1-12. (January 2022)
Materia
Enfermedades de las Plantas
Ascochyta rabiei
Tizón
Garbanzo
Enfermedades Foliares
Fitopatología
Plant Diseases
Blight
Chickpeas
Foliar Diseases
Plant Pathology
Didymella rabiei
Pathogenic Variability
Mating types
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11131
Acceder
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oai_identifier_str oai:localhost:20.500.12123/11131
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in ArgentinaCrociara, Clara SoniaValetti, LucioBernanrdi Lima, NelsonIglesias, JulianaPastor, SilvinaEnfermedades de las PlantasAscochyta rabieiTizónGarbanzoEnfermedades FoliaresFitopatologíaPlant DiseasesBlightChickpeasFoliar DiseasesPlant PathologyDidymella rabieiPathogenic VariabilityMating typesAscochyta blight is one of the most devastating foliar diseases of chickpea. The causal agent, Ascochyta rabiei, is a heterothallic ascomycete and its sexual reproduction depends on the proximate presence of both mating types, MAT1-1 and MAT1-2. Since its first detection in Argentina in 2011, very few studies have been carried out and information on local isolates remains unknown. In this work, 12 isolates were obtained from the northern, central and southern regions of Córdoba province, Argentina. First, isolates were tentatively identified as A. rabiei based on their colony and conidial characteristics. Identity was confirmed by phylogenetic analysis of the ITS, β-tubulin and D1/D2 loci. Morphology, cultural characteristics and pathogenic variability of the isolates were compared. Likewise, 27 crosses were made to demonstrate possible sexual reproduction between the strains. The isolates showed differences in morphology (shape, texture, conidial size, colony colour, growth rate) and aggressiveness from a susceptible cultivar. All crosses were fertile and produced pseudothecia, asci and ascospores. This work is the first in Latin America to characterize isolates of A. rabiei morphologically and molecularly, examine their pathogenic variability and demonstrate the sexual reproduction between isolates carrying different mating-type idiomorphs. New levels of aggressiveness in A. rabiei or adaptive changes like resistance to fungicides or breakdown of host resistance are a distinct possibility in the future.EEA PergaminoFil: Crociara, Clara Sonia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Crociara, Clara Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Bernardi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Bernardi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Iglesias, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Departamento de Maíz; ArgentinaFil: Iglesias, Juliana. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Agrarias, Naturales y Ambientales; ArgentinaFil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Pastor, Silvina Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaWiley2022-02-14T10:43:31Z2022-02-14T10:43:31Z2022-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11131https://onlinelibrary.wiley.com/doi/10.1111/jph.130731439-0434 (online)https://doi.org/10.1111/jph.13073Journal of Phytopathology 170 (1) : 1-12. (January 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E6-I132-001/2019-PE-E6-I132-001/AR./Desarrollo de legumbres adaptadas a los territorios y diferenciados por calidad comercial, nutricional e industrial.info:eu-repograntAgreement/INTA/2019-PD-E4-I090-001/2019-PD-E4-I090-001/AR./Análisis de patosistemas en cultivos agrícolas y especies forestales. Caracterización de sus componentesinfo:eu-repo/semantics/restrictedAccess2025-11-06T09:41:06Zoai:localhost:20.500.12123/11131instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-06 09:41:07.12INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina
title Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina
spellingShingle Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina
Crociara, Clara Sonia
Enfermedades de las Plantas
Ascochyta rabiei
Tizón
Garbanzo
Enfermedades Foliares
Fitopatología
Plant Diseases
Blight
Chickpeas
Foliar Diseases
Plant Pathology
Didymella rabiei
Pathogenic Variability
Mating types
title_short Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina
title_full Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina
title_fullStr Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina
title_full_unstemmed Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina
title_sort Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina
dc.creator.none.fl_str_mv Crociara, Clara Sonia
Valetti, Lucio
Bernanrdi Lima, Nelson
Iglesias, Juliana
Pastor, Silvina
author Crociara, Clara Sonia
author_facet Crociara, Clara Sonia
Valetti, Lucio
Bernanrdi Lima, Nelson
Iglesias, Juliana
Pastor, Silvina
author_role author
author2 Valetti, Lucio
Bernanrdi Lima, Nelson
Iglesias, Juliana
Pastor, Silvina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de las Plantas
Ascochyta rabiei
Tizón
Garbanzo
Enfermedades Foliares
Fitopatología
Plant Diseases
Blight
Chickpeas
Foliar Diseases
Plant Pathology
Didymella rabiei
Pathogenic Variability
Mating types
topic Enfermedades de las Plantas
Ascochyta rabiei
Tizón
Garbanzo
Enfermedades Foliares
Fitopatología
Plant Diseases
Blight
Chickpeas
Foliar Diseases
Plant Pathology
Didymella rabiei
Pathogenic Variability
Mating types
dc.description.none.fl_txt_mv Ascochyta blight is one of the most devastating foliar diseases of chickpea. The causal agent, Ascochyta rabiei, is a heterothallic ascomycete and its sexual reproduction depends on the proximate presence of both mating types, MAT1-1 and MAT1-2. Since its first detection in Argentina in 2011, very few studies have been carried out and information on local isolates remains unknown. In this work, 12 isolates were obtained from the northern, central and southern regions of Córdoba province, Argentina. First, isolates were tentatively identified as A. rabiei based on their colony and conidial characteristics. Identity was confirmed by phylogenetic analysis of the ITS, β-tubulin and D1/D2 loci. Morphology, cultural characteristics and pathogenic variability of the isolates were compared. Likewise, 27 crosses were made to demonstrate possible sexual reproduction between the strains. The isolates showed differences in morphology (shape, texture, conidial size, colony colour, growth rate) and aggressiveness from a susceptible cultivar. All crosses were fertile and produced pseudothecia, asci and ascospores. This work is the first in Latin America to characterize isolates of A. rabiei morphologically and molecularly, examine their pathogenic variability and demonstrate the sexual reproduction between isolates carrying different mating-type idiomorphs. New levels of aggressiveness in A. rabiei or adaptive changes like resistance to fungicides or breakdown of host resistance are a distinct possibility in the future.
EEA Pergamino
Fil: Crociara, Clara Sonia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Crociara, Clara Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Bernardi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bernardi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Iglesias, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Departamento de Maíz; Argentina
Fil: Iglesias, Juliana. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Agrarias, Naturales y Ambientales; Argentina
Fil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Pastor, Silvina Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
description Ascochyta blight is one of the most devastating foliar diseases of chickpea. The causal agent, Ascochyta rabiei, is a heterothallic ascomycete and its sexual reproduction depends on the proximate presence of both mating types, MAT1-1 and MAT1-2. Since its first detection in Argentina in 2011, very few studies have been carried out and information on local isolates remains unknown. In this work, 12 isolates were obtained from the northern, central and southern regions of Córdoba province, Argentina. First, isolates were tentatively identified as A. rabiei based on their colony and conidial characteristics. Identity was confirmed by phylogenetic analysis of the ITS, β-tubulin and D1/D2 loci. Morphology, cultural characteristics and pathogenic variability of the isolates were compared. Likewise, 27 crosses were made to demonstrate possible sexual reproduction between the strains. The isolates showed differences in morphology (shape, texture, conidial size, colony colour, growth rate) and aggressiveness from a susceptible cultivar. All crosses were fertile and produced pseudothecia, asci and ascospores. This work is the first in Latin America to characterize isolates of A. rabiei morphologically and molecularly, examine their pathogenic variability and demonstrate the sexual reproduction between isolates carrying different mating-type idiomorphs. New levels of aggressiveness in A. rabiei or adaptive changes like resistance to fungicides or breakdown of host resistance are a distinct possibility in the future.
publishDate 2022
dc.date.none.fl_str_mv 2022-02-14T10:43:31Z
2022-02-14T10:43:31Z
2022-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11131
https://onlinelibrary.wiley.com/doi/10.1111/jph.13073
1439-0434 (online)
https://doi.org/10.1111/jph.13073
url http://hdl.handle.net/20.500.12123/11131
https://onlinelibrary.wiley.com/doi/10.1111/jph.13073
https://doi.org/10.1111/jph.13073
identifier_str_mv 1439-0434 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E6-I132-001/2019-PE-E6-I132-001/AR./Desarrollo de legumbres adaptadas a los territorios y diferenciados por calidad comercial, nutricional e industrial.
info:eu-repograntAgreement/INTA/2019-PD-E4-I090-001/2019-PD-E4-I090-001/AR./Análisis de patosistemas en cultivos agrícolas y especies forestales. Caracterización de sus componentes
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Journal of Phytopathology 170 (1) : 1-12. (January 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.082534

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