Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle

Autores
Larzabal, Mariano; Marques Da Silva, Wanderson; Multani, Anmol; Vagnoni, Lucas Emilio; Moore, Prando Dadin; Marin, Maia Solange; Riviere, Nahuel Agustin; Delgado, Fernando Oscar; Vilte, Daniel Alejandro; Romero Victorica, Matias; Tao, Ma; Luo, Le Guan; Talia, Paola Mónica; Cataldi, Angel Adrian; Cobo, Eduardo Rubén
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The zoonotic enterohemorrhagic Escherichia coli (EHEC) O157: H7 bacterium causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS) in humans. Cattle are primary reservoirs and EHEC O157: H7; the bacteria predominately inhabit the colon and recto-anal junctions (RAJ). The early innate immune reactions in the infected gut are critical in the pathogenesis of EHEC O157: H7. In this study, calves orally inoculated with EHEC O157: H7 showed infiltration of neutrophils in the lamina propria of ileum and RAJ at 7 and 14 days post-infection. Infected calves had altered mucin layer and mast cell populations across small and large intestines. There were differential transcription expressions of key bovine β defensins, tracheal antimicrobial peptide (TAP) in the ileum, and lingual antimicrobial peptide (LAP) in RAJ. The main Gram-negative bacterial/LPS signaling Toll-Like receptor 4 (TLR4) was downregulated in RAJ. Intestinal infection with EHEC O157: H7 impacted the gut bacterial communities and influenced the relative abundance of Negativibacillus and Erysipelotrichaceae in mucosa-associated bacteria in the rectum. Thus, innate immunity in the gut of calves showed unique characteristics during infection with EHEC O157: H7, which occurred in the absence of major clinical manifestations but denoted an active immunological niche.
Instituto de Biotecnología
Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular, Argentina
Fil: Marques Da Silva, Wanderson. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Multani, Anmol. University of Calgary. Faculty Veterinary Medicine. Production Animal Health; Canadá
Fil: Vagnoni, Lucas Emilio. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Vagnoni, Lucas Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marin, Maia Solange. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Marin, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Riviere, Nahuel Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Riviere, Nahuel Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Delgado, Fernando Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Vilte, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero Victorica, Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Romero Victorica, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tao, Ma. University of Alberta. Department of Agricultural, Food and Nutritional Science; Canadá
Fil: Tao, Ma. Chinese Academy of Agricultural Sciences. Feed Research Institute/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs; China
Fil: Luo, Le Guan. University of Alberta. Department of Agricultural, Food and Nutritional Science; Canadá
Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Talia, Paola Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cobo, Eduardo Rubén. University of Calgary. Faculty of Veterinary Medicine. Production Animal Health; Canadá
Fuente
Scientific Reports 10 : Article number: 21535 (2020)
Materia
Ganado Bovino
Escherichia coli
Inmunología
Enfermedades de los Animales
Enfermedades Intestinales
Cattle
Immunology
Animal Diseases
Intestinal Diseases
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10877
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10877
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network_name_str INTA Digital (INTA)
spelling Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattleLarzabal, MarianoMarques Da Silva, WandersonMultani, AnmolVagnoni, Lucas EmilioMoore, Prando DadinMarin, Maia SolangeRiviere, Nahuel AgustinDelgado, Fernando OscarVilte, Daniel AlejandroRomero Victorica, MatiasTao, MaLuo, Le GuanTalia, Paola MónicaCataldi, Angel AdrianCobo, Eduardo RubénGanado BovinoEscherichia coliInmunologíaEnfermedades de los AnimalesEnfermedades IntestinalesCattleImmunologyAnimal DiseasesIntestinal DiseasesThe zoonotic enterohemorrhagic Escherichia coli (EHEC) O157: H7 bacterium causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS) in humans. Cattle are primary reservoirs and EHEC O157: H7; the bacteria predominately inhabit the colon and recto-anal junctions (RAJ). The early innate immune reactions in the infected gut are critical in the pathogenesis of EHEC O157: H7. In this study, calves orally inoculated with EHEC O157: H7 showed infiltration of neutrophils in the lamina propria of ileum and RAJ at 7 and 14 days post-infection. Infected calves had altered mucin layer and mast cell populations across small and large intestines. There were differential transcription expressions of key bovine β defensins, tracheal antimicrobial peptide (TAP) in the ileum, and lingual antimicrobial peptide (LAP) in RAJ. The main Gram-negative bacterial/LPS signaling Toll-Like receptor 4 (TLR4) was downregulated in RAJ. Intestinal infection with EHEC O157: H7 impacted the gut bacterial communities and influenced the relative abundance of Negativibacillus and Erysipelotrichaceae in mucosa-associated bacteria in the rectum. Thus, innate immunity in the gut of calves showed unique characteristics during infection with EHEC O157: H7, which occurred in the absence of major clinical manifestations but denoted an active immunological niche.Instituto de BiotecnologíaFil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular, ArgentinaFil: Marques Da Silva, Wanderson. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Multani, Anmol. University of Calgary. Faculty Veterinary Medicine. Production Animal Health; CanadáFil: Vagnoni, Lucas Emilio. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Vagnoni, Lucas Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marin, Maia Solange. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Marin, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Riviere, Nahuel Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Riviere, Nahuel Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Delgado, Fernando Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; ArgentinaFil: Vilte, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romero Victorica, Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Romero Victorica, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tao, Ma. University of Alberta. Department of Agricultural, Food and Nutritional Science; CanadáFil: Tao, Ma. Chinese Academy of Agricultural Sciences. Feed Research Institute/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs; ChinaFil: Luo, Le Guan. University of Alberta. Department of Agricultural, Food and Nutritional Science; CanadáFil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Talia, Paola Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cobo, Eduardo Rubén. University of Calgary. Faculty of Veterinary Medicine. Production Animal Health; CanadáSpringerNature2021-12-09T18:05:36Z2021-12-09T18:05:36Z2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10877https://www.nature.com/articles/s41598-020-78752-x2045-2322https://doi.org/10.1038/s41598-020-78752-xScientific Reports 10 : Article number: 21535 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:25Zoai:localhost:20.500.12123/10877instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:26.074INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle
title Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle
spellingShingle Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle
Larzabal, Mariano
Ganado Bovino
Escherichia coli
Inmunología
Enfermedades de los Animales
Enfermedades Intestinales
Cattle
Immunology
Animal Diseases
Intestinal Diseases
title_short Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle
title_full Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle
title_fullStr Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle
title_full_unstemmed Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle
title_sort Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle
dc.creator.none.fl_str_mv Larzabal, Mariano
Marques Da Silva, Wanderson
Multani, Anmol
Vagnoni, Lucas Emilio
Moore, Prando Dadin
Marin, Maia Solange
Riviere, Nahuel Agustin
Delgado, Fernando Oscar
Vilte, Daniel Alejandro
Romero Victorica, Matias
Tao, Ma
Luo, Le Guan
Talia, Paola Mónica
Cataldi, Angel Adrian
Cobo, Eduardo Rubén
author Larzabal, Mariano
author_facet Larzabal, Mariano
Marques Da Silva, Wanderson
Multani, Anmol
Vagnoni, Lucas Emilio
Moore, Prando Dadin
Marin, Maia Solange
Riviere, Nahuel Agustin
Delgado, Fernando Oscar
Vilte, Daniel Alejandro
Romero Victorica, Matias
Tao, Ma
Luo, Le Guan
Talia, Paola Mónica
Cataldi, Angel Adrian
Cobo, Eduardo Rubén
author_role author
author2 Marques Da Silva, Wanderson
Multani, Anmol
Vagnoni, Lucas Emilio
Moore, Prando Dadin
Marin, Maia Solange
Riviere, Nahuel Agustin
Delgado, Fernando Oscar
Vilte, Daniel Alejandro
Romero Victorica, Matias
Tao, Ma
Luo, Le Guan
Talia, Paola Mónica
Cataldi, Angel Adrian
Cobo, Eduardo Rubén
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Escherichia coli
Inmunología
Enfermedades de los Animales
Enfermedades Intestinales
Cattle
Immunology
Animal Diseases
Intestinal Diseases
topic Ganado Bovino
Escherichia coli
Inmunología
Enfermedades de los Animales
Enfermedades Intestinales
Cattle
Immunology
Animal Diseases
Intestinal Diseases
dc.description.none.fl_txt_mv The zoonotic enterohemorrhagic Escherichia coli (EHEC) O157: H7 bacterium causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS) in humans. Cattle are primary reservoirs and EHEC O157: H7; the bacteria predominately inhabit the colon and recto-anal junctions (RAJ). The early innate immune reactions in the infected gut are critical in the pathogenesis of EHEC O157: H7. In this study, calves orally inoculated with EHEC O157: H7 showed infiltration of neutrophils in the lamina propria of ileum and RAJ at 7 and 14 days post-infection. Infected calves had altered mucin layer and mast cell populations across small and large intestines. There were differential transcription expressions of key bovine β defensins, tracheal antimicrobial peptide (TAP) in the ileum, and lingual antimicrobial peptide (LAP) in RAJ. The main Gram-negative bacterial/LPS signaling Toll-Like receptor 4 (TLR4) was downregulated in RAJ. Intestinal infection with EHEC O157: H7 impacted the gut bacterial communities and influenced the relative abundance of Negativibacillus and Erysipelotrichaceae in mucosa-associated bacteria in the rectum. Thus, innate immunity in the gut of calves showed unique characteristics during infection with EHEC O157: H7, which occurred in the absence of major clinical manifestations but denoted an active immunological niche.
Instituto de Biotecnología
Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular, Argentina
Fil: Marques Da Silva, Wanderson. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Multani, Anmol. University of Calgary. Faculty Veterinary Medicine. Production Animal Health; Canadá
Fil: Vagnoni, Lucas Emilio. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Vagnoni, Lucas Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marin, Maia Solange. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Marin, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Riviere, Nahuel Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Riviere, Nahuel Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Delgado, Fernando Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Vilte, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero Victorica, Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Romero Victorica, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tao, Ma. University of Alberta. Department of Agricultural, Food and Nutritional Science; Canadá
Fil: Tao, Ma. Chinese Academy of Agricultural Sciences. Feed Research Institute/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs; China
Fil: Luo, Le Guan. University of Alberta. Department of Agricultural, Food and Nutritional Science; Canadá
Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Talia, Paola Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cobo, Eduardo Rubén. University of Calgary. Faculty of Veterinary Medicine. Production Animal Health; Canadá
description The zoonotic enterohemorrhagic Escherichia coli (EHEC) O157: H7 bacterium causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS) in humans. Cattle are primary reservoirs and EHEC O157: H7; the bacteria predominately inhabit the colon and recto-anal junctions (RAJ). The early innate immune reactions in the infected gut are critical in the pathogenesis of EHEC O157: H7. In this study, calves orally inoculated with EHEC O157: H7 showed infiltration of neutrophils in the lamina propria of ileum and RAJ at 7 and 14 days post-infection. Infected calves had altered mucin layer and mast cell populations across small and large intestines. There were differential transcription expressions of key bovine β defensins, tracheal antimicrobial peptide (TAP) in the ileum, and lingual antimicrobial peptide (LAP) in RAJ. The main Gram-negative bacterial/LPS signaling Toll-Like receptor 4 (TLR4) was downregulated in RAJ. Intestinal infection with EHEC O157: H7 impacted the gut bacterial communities and influenced the relative abundance of Negativibacillus and Erysipelotrichaceae in mucosa-associated bacteria in the rectum. Thus, innate immunity in the gut of calves showed unique characteristics during infection with EHEC O157: H7, which occurred in the absence of major clinical manifestations but denoted an active immunological niche.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
2021-12-09T18:05:36Z
2021-12-09T18:05:36Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10877
https://www.nature.com/articles/s41598-020-78752-x
2045-2322
https://doi.org/10.1038/s41598-020-78752-x
url http://hdl.handle.net/20.500.12123/10877
https://www.nature.com/articles/s41598-020-78752-x
https://doi.org/10.1038/s41598-020-78752-x
identifier_str_mv 2045-2322
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv SpringerNature
publisher.none.fl_str_mv SpringerNature
dc.source.none.fl_str_mv Scientific Reports 10 : Article number: 21535 (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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