Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina

Autores
Del Castillo, Lourdes; D’Astek, Beatriz A.; Miliwebsky, Elizabeth; Carbonari, Claudia Carolina; Palladino, Pablo Martin; Deza, Natalia; Chinen, Isabel; Manfredi, Eduardo; Leotta, Gerardo A.; Masana, Marcelo Oscar; Rivas, Marta
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Shiga toxin–producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI– pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n = 122), BD (n = 69), and NBD (n = 30) cases, and healthy carriers (n = 5), and from bovines (n = 54) in the abattoirs. The stx-genotyping showed that stx2/stx2c(vh-a) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx2 (20.8%) in human and stx2c(vh-a) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx2/stx2c(vh-a) (12 humans and one bovine), PT4-AREXH01.0543-stx2/stx2c(vh-a) (one human and four bovines), PT2-AREXH01.0076-stx2/stx2c(vh-a) (one human and four bovines), PT49-AREXH01.0175-stx2/stx2c(vh-a) (seven humans and one bovine), and PT49- AREXH01.0022-stx2/stx2c(vh-a) (seven humans and one bovine). No correlation was found among the stx-genotypes, the phage type, and the clinical symptoms.
Fil: Del Castillo, Lourdes L. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.
Fil: D’Astek, Beatriz A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Miliwebsky, Elizabeth. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Carbonari, Claudia Carolina. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Palladino, Pablo Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.
Fil: Deza, Natalia. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Chinen, Isabel. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Manfredi, Eduardo. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Leotta, Gerardo A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Masana, Marcelo Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.
Fil: Rivas, Marta. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fuente
Foodborne Pathogens and Disease 9 (5) : 457-464 (2012)
Materia
Escherichia coli
Ganado Bovino
Cattle
Animal Diseases
Identification
Enfermedades de los Animales
Identificación
Argentina
Escherichia coli O157:H7
Strains Isolated
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5063
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5063
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in ArgentinaDel Castillo, LourdesD’Astek, Beatriz A.Miliwebsky, ElizabethCarbonari, Claudia CarolinaPalladino, Pablo MartinDeza, NataliaChinen, IsabelManfredi, EduardoLeotta, Gerardo A.Masana, Marcelo OscarRivas, MartaEscherichia coliGanado BovinoCattleAnimal DiseasesIdentificationEnfermedades de los AnimalesIdentificaciónArgentinaEscherichia coli O157:H7Strains IsolatedShiga toxin–producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI– pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n = 122), BD (n = 69), and NBD (n = 30) cases, and healthy carriers (n = 5), and from bovines (n = 54) in the abattoirs. The stx-genotyping showed that stx2/stx2c(vh-a) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx2 (20.8%) in human and stx2c(vh-a) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx2/stx2c(vh-a) (12 humans and one bovine), PT4-AREXH01.0543-stx2/stx2c(vh-a) (one human and four bovines), PT2-AREXH01.0076-stx2/stx2c(vh-a) (one human and four bovines), PT49-AREXH01.0175-stx2/stx2c(vh-a) (seven humans and one bovine), and PT49- AREXH01.0022-stx2/stx2c(vh-a) (seven humans and one bovine). No correlation was found among the stx-genotypes, the phage type, and the clinical symptoms.Fil: Del Castillo, Lourdes L. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.Fil: D’Astek, Beatriz A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Fil: Miliwebsky, Elizabeth. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Fil: Carbonari, Claudia Carolina. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Fil: Palladino, Pablo Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.Fil: Deza, Natalia. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Fil: Chinen, Isabel. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Fil: Manfredi, Eduardo. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Fil: Leotta, Gerardo A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Masana, Marcelo Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.Fil: Rivas, Marta. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Mary Ann Liebert, Inc.2019-05-08T11:05:38Z2019-05-08T11:05:38Z2012-05-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.liebertpub.com/doi/abs/10.1089/fpd.2011.1062http://hdl.handle.net/20.500.12123/50631535-31411556-7125http://doi.org/10.1089/fpd.2011.1062Foodborne Pathogens and Disease 9 (5) : 457-464 (2012)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:16:56Zoai:localhost:20.500.12123/5063instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:57.133INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina
title Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina
spellingShingle Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina
Del Castillo, Lourdes
Escherichia coli
Ganado Bovino
Cattle
Animal Diseases
Identification
Enfermedades de los Animales
Identificación
Argentina
Escherichia coli O157:H7
Strains Isolated
title_short Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina
title_full Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina
title_fullStr Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina
title_full_unstemmed Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina
title_sort Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina
dc.creator.none.fl_str_mv Del Castillo, Lourdes
D’Astek, Beatriz A.
Miliwebsky, Elizabeth
Carbonari, Claudia Carolina
Palladino, Pablo Martin
Deza, Natalia
Chinen, Isabel
Manfredi, Eduardo
Leotta, Gerardo A.
Masana, Marcelo Oscar
Rivas, Marta
author Del Castillo, Lourdes
author_facet Del Castillo, Lourdes
D’Astek, Beatriz A.
Miliwebsky, Elizabeth
Carbonari, Claudia Carolina
Palladino, Pablo Martin
Deza, Natalia
Chinen, Isabel
Manfredi, Eduardo
Leotta, Gerardo A.
Masana, Marcelo Oscar
Rivas, Marta
author_role author
author2 D’Astek, Beatriz A.
Miliwebsky, Elizabeth
Carbonari, Claudia Carolina
Palladino, Pablo Martin
Deza, Natalia
Chinen, Isabel
Manfredi, Eduardo
Leotta, Gerardo A.
Masana, Marcelo Oscar
Rivas, Marta
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Escherichia coli
Ganado Bovino
Cattle
Animal Diseases
Identification
Enfermedades de los Animales
Identificación
Argentina
Escherichia coli O157:H7
Strains Isolated
topic Escherichia coli
Ganado Bovino
Cattle
Animal Diseases
Identification
Enfermedades de los Animales
Identificación
Argentina
Escherichia coli O157:H7
Strains Isolated
dc.description.none.fl_txt_mv Shiga toxin–producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI– pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n = 122), BD (n = 69), and NBD (n = 30) cases, and healthy carriers (n = 5), and from bovines (n = 54) in the abattoirs. The stx-genotyping showed that stx2/stx2c(vh-a) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx2 (20.8%) in human and stx2c(vh-a) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx2/stx2c(vh-a) (12 humans and one bovine), PT4-AREXH01.0543-stx2/stx2c(vh-a) (one human and four bovines), PT2-AREXH01.0076-stx2/stx2c(vh-a) (one human and four bovines), PT49-AREXH01.0175-stx2/stx2c(vh-a) (seven humans and one bovine), and PT49- AREXH01.0022-stx2/stx2c(vh-a) (seven humans and one bovine). No correlation was found among the stx-genotypes, the phage type, and the clinical symptoms.
Fil: Del Castillo, Lourdes L. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.
Fil: D’Astek, Beatriz A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Miliwebsky, Elizabeth. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Carbonari, Claudia Carolina. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Palladino, Pablo Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.
Fil: Deza, Natalia. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Chinen, Isabel. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Manfredi, Eduardo. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Leotta, Gerardo A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Masana, Marcelo Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.
Fil: Rivas, Marta. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
description Shiga toxin–producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI– pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n = 122), BD (n = 69), and NBD (n = 30) cases, and healthy carriers (n = 5), and from bovines (n = 54) in the abattoirs. The stx-genotyping showed that stx2/stx2c(vh-a) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx2 (20.8%) in human and stx2c(vh-a) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx2/stx2c(vh-a) (12 humans and one bovine), PT4-AREXH01.0543-stx2/stx2c(vh-a) (one human and four bovines), PT2-AREXH01.0076-stx2/stx2c(vh-a) (one human and four bovines), PT49-AREXH01.0175-stx2/stx2c(vh-a) (seven humans and one bovine), and PT49- AREXH01.0022-stx2/stx2c(vh-a) (seven humans and one bovine). No correlation was found among the stx-genotypes, the phage type, and the clinical symptoms.
publishDate 2012
dc.date.none.fl_str_mv 2012-05-02
2019-05-08T11:05:38Z
2019-05-08T11:05:38Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.liebertpub.com/doi/abs/10.1089/fpd.2011.1062
http://hdl.handle.net/20.500.12123/5063
1535-3141
1556-7125
http://doi.org/10.1089/fpd.2011.1062
url https://www.liebertpub.com/doi/abs/10.1089/fpd.2011.1062
http://hdl.handle.net/20.500.12123/5063
http://doi.org/10.1089/fpd.2011.1062
identifier_str_mv 1535-3141
1556-7125
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert, Inc.
publisher.none.fl_str_mv Mary Ann Liebert, Inc.
dc.source.none.fl_str_mv Foodborne Pathogens and Disease 9 (5) : 457-464 (2012)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
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instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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