Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina
- Autores
- Del Castillo, Lourdes; D’Astek, Beatriz A.; Miliwebsky, Elizabeth; Carbonari, Claudia Carolina; Palladino, Pablo Martin; Deza, Natalia; Chinen, Isabel; Manfredi, Eduardo; Leotta, Gerardo A.; Masana, Marcelo Oscar; Rivas, Marta
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Shiga toxin–producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI– pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n = 122), BD (n = 69), and NBD (n = 30) cases, and healthy carriers (n = 5), and from bovines (n = 54) in the abattoirs. The stx-genotyping showed that stx2/stx2c(vh-a) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx2 (20.8%) in human and stx2c(vh-a) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx2/stx2c(vh-a) (12 humans and one bovine), PT4-AREXH01.0543-stx2/stx2c(vh-a) (one human and four bovines), PT2-AREXH01.0076-stx2/stx2c(vh-a) (one human and four bovines), PT49-AREXH01.0175-stx2/stx2c(vh-a) (seven humans and one bovine), and PT49- AREXH01.0022-stx2/stx2c(vh-a) (seven humans and one bovine). No correlation was found among the stx-genotypes, the phage type, and the clinical symptoms.
Fil: Del Castillo, Lourdes L. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.
Fil: D’Astek, Beatriz A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Miliwebsky, Elizabeth. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Carbonari, Claudia Carolina. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Palladino, Pablo Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.
Fil: Deza, Natalia. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Chinen, Isabel. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Manfredi, Eduardo. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.
Fil: Leotta, Gerardo A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Masana, Marcelo Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.
Fil: Rivas, Marta. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. - Fuente
- Foodborne Pathogens and Disease 9 (5) : 457-464 (2012)
- Materia
-
Escherichia coli
Ganado Bovino
Cattle
Animal Diseases
Identification
Enfermedades de los Animales
Identificación
Argentina
Escherichia coli O157:H7
Strains Isolated - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/5063
Ver los metadatos del registro completo
id |
INTADig_26b787bb34e6825faf4ffd2abe08353c |
---|---|
oai_identifier_str |
oai:localhost:20.500.12123/5063 |
network_acronym_str |
INTADig |
repository_id_str |
l |
network_name_str |
INTA Digital (INTA) |
spelling |
Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in ArgentinaDel Castillo, LourdesD’Astek, Beatriz A.Miliwebsky, ElizabethCarbonari, Claudia CarolinaPalladino, Pablo MartinDeza, NataliaChinen, IsabelManfredi, EduardoLeotta, Gerardo A.Masana, Marcelo OscarRivas, MartaEscherichia coliGanado BovinoCattleAnimal DiseasesIdentificationEnfermedades de los AnimalesIdentificaciónArgentinaEscherichia coli O157:H7Strains IsolatedShiga toxin–producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI– pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n = 122), BD (n = 69), and NBD (n = 30) cases, and healthy carriers (n = 5), and from bovines (n = 54) in the abattoirs. The stx-genotyping showed that stx2/stx2c(vh-a) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx2 (20.8%) in human and stx2c(vh-a) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx2/stx2c(vh-a) (12 humans and one bovine), PT4-AREXH01.0543-stx2/stx2c(vh-a) (one human and four bovines), PT2-AREXH01.0076-stx2/stx2c(vh-a) (one human and four bovines), PT49-AREXH01.0175-stx2/stx2c(vh-a) (seven humans and one bovine), and PT49- AREXH01.0022-stx2/stx2c(vh-a) (seven humans and one bovine). No correlation was found among the stx-genotypes, the phage type, and the clinical symptoms.Fil: Del Castillo, Lourdes L. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.Fil: D’Astek, Beatriz A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Fil: Miliwebsky, Elizabeth. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Fil: Carbonari, Claudia Carolina. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Fil: Palladino, Pablo Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.Fil: Deza, Natalia. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Fil: Chinen, Isabel. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Fil: Manfredi, Eduardo. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Fil: Leotta, Gerardo A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Masana, Marcelo Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina.Fil: Rivas, Marta. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina.Mary Ann Liebert, Inc.2019-05-08T11:05:38Z2019-05-08T11:05:38Z2012-05-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.liebertpub.com/doi/abs/10.1089/fpd.2011.1062http://hdl.handle.net/20.500.12123/50631535-31411556-7125http://doi.org/10.1089/fpd.2011.1062Foodborne Pathogens and Disease 9 (5) : 457-464 (2012)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:39Zoai:localhost:20.500.12123/5063instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:40.114INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina |
title |
Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina |
spellingShingle |
Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina Del Castillo, Lourdes Escherichia coli Ganado Bovino Cattle Animal Diseases Identification Enfermedades de los Animales Identificación Argentina Escherichia coli O157:H7 Strains Isolated |
title_short |
Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina |
title_full |
Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina |
title_fullStr |
Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina |
title_full_unstemmed |
Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina |
title_sort |
Subtyping of Escherichia coli O157:H7 Strains Isolated from Human Infections and Healthy Cattle in Argentina |
dc.creator.none.fl_str_mv |
Del Castillo, Lourdes D’Astek, Beatriz A. Miliwebsky, Elizabeth Carbonari, Claudia Carolina Palladino, Pablo Martin Deza, Natalia Chinen, Isabel Manfredi, Eduardo Leotta, Gerardo A. Masana, Marcelo Oscar Rivas, Marta |
author |
Del Castillo, Lourdes |
author_facet |
Del Castillo, Lourdes D’Astek, Beatriz A. Miliwebsky, Elizabeth Carbonari, Claudia Carolina Palladino, Pablo Martin Deza, Natalia Chinen, Isabel Manfredi, Eduardo Leotta, Gerardo A. Masana, Marcelo Oscar Rivas, Marta |
author_role |
author |
author2 |
D’Astek, Beatriz A. Miliwebsky, Elizabeth Carbonari, Claudia Carolina Palladino, Pablo Martin Deza, Natalia Chinen, Isabel Manfredi, Eduardo Leotta, Gerardo A. Masana, Marcelo Oscar Rivas, Marta |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Escherichia coli Ganado Bovino Cattle Animal Diseases Identification Enfermedades de los Animales Identificación Argentina Escherichia coli O157:H7 Strains Isolated |
topic |
Escherichia coli Ganado Bovino Cattle Animal Diseases Identification Enfermedades de los Animales Identificación Argentina Escherichia coli O157:H7 Strains Isolated |
dc.description.none.fl_txt_mv |
Shiga toxin–producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI– pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n = 122), BD (n = 69), and NBD (n = 30) cases, and healthy carriers (n = 5), and from bovines (n = 54) in the abattoirs. The stx-genotyping showed that stx2/stx2c(vh-a) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx2 (20.8%) in human and stx2c(vh-a) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx2/stx2c(vh-a) (12 humans and one bovine), PT4-AREXH01.0543-stx2/stx2c(vh-a) (one human and four bovines), PT2-AREXH01.0076-stx2/stx2c(vh-a) (one human and four bovines), PT49-AREXH01.0175-stx2/stx2c(vh-a) (seven humans and one bovine), and PT49- AREXH01.0022-stx2/stx2c(vh-a) (seven humans and one bovine). No correlation was found among the stx-genotypes, the phage type, and the clinical symptoms. Fil: Del Castillo, Lourdes L. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Fil: D’Astek, Beatriz A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Fil: Miliwebsky, Elizabeth. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Fil: Carbonari, Claudia Carolina. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Fil: Palladino, Pablo Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Fil: Deza, Natalia. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Fil: Chinen, Isabel. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Fil: Manfredi, Eduardo. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Fil: Leotta, Gerardo A. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Masana, Marcelo Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Fil: Rivas, Marta. Instituto Nacional de Enfermedades Infecciosas-ANLIS ‘‘Dr. Carlos G. Malbrán’’. Servicio Fisiopatogenia; Argentina. |
description |
Shiga toxin–producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI– pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n = 122), BD (n = 69), and NBD (n = 30) cases, and healthy carriers (n = 5), and from bovines (n = 54) in the abattoirs. The stx-genotyping showed that stx2/stx2c(vh-a) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx2 (20.8%) in human and stx2c(vh-a) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx2/stx2c(vh-a) (12 humans and one bovine), PT4-AREXH01.0543-stx2/stx2c(vh-a) (one human and four bovines), PT2-AREXH01.0076-stx2/stx2c(vh-a) (one human and four bovines), PT49-AREXH01.0175-stx2/stx2c(vh-a) (seven humans and one bovine), and PT49- AREXH01.0022-stx2/stx2c(vh-a) (seven humans and one bovine). No correlation was found among the stx-genotypes, the phage type, and the clinical symptoms. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-05-02 2019-05-08T11:05:38Z 2019-05-08T11:05:38Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://www.liebertpub.com/doi/abs/10.1089/fpd.2011.1062 http://hdl.handle.net/20.500.12123/5063 1535-3141 1556-7125 http://doi.org/10.1089/fpd.2011.1062 |
url |
https://www.liebertpub.com/doi/abs/10.1089/fpd.2011.1062 http://hdl.handle.net/20.500.12123/5063 http://doi.org/10.1089/fpd.2011.1062 |
identifier_str_mv |
1535-3141 1556-7125 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert, Inc. |
publisher.none.fl_str_mv |
Mary Ann Liebert, Inc. |
dc.source.none.fl_str_mv |
Foodborne Pathogens and Disease 9 (5) : 457-464 (2012) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
_version_ |
1844619133484793856 |
score |
12.559606 |