Overexpressed proteins in hypervirulent clade 8 and clade 6 strains of Escherichia coli O157:H7 compared to E. coli O157:H7 EDL933 clade 3 strain

Autores
Amigo, Natalia; Qi, Zhang; Amadio, Ariel; Qunjie, Zhang; Marques da Silva, Wanderson; Baiyuan, Cui; Zhongjian, Chen; Larzabal, Mariano; Cataldi, Angel Adrian; Jinlong, Bei
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Escherichia coli O157:H7 is responsible for severe diarrhea and hemolytic uremic syndrome (HUS), and predominantly affects children under 5 years. The major virulence traits are Shiga toxins, necessary to develop HUS and the Type III Secretion System (T3SS) through which bacteria translocate effector proteins directly into the host cell. By SNPs typing, E. coli O157:H7 was separated into nine different clades. Clade 8 and clade 6 strains were more frequently associated with severe disease and HUS. In this study, we aimed to identify differentially expressed proteins in two strains of E. coli O157:H7 (clade 8 and clade 6), obtained from cattle and compared them with the well characterized reference EDL933 strain (clade 3). Clade 8 and clade 6 strains show enhanced pathogenicity in a mouse model and virulence-related properties. Proteins were extracted and analyzed using the TMT-6plex labeling strategy associated with two dimensional liquid chromatography and mass spectrometry in tandem. We detected 2241 proteins in the cell extract and 1787 proteins in the culture supernatants. Attention was focused on the proteins related to virulence, overexpressed in clade 6 and 8 strains compared to EDL933 strain. The proteins relevant overexpressed in clade 8 strain were the curli protein CsgC, a transcriptional activator (PchE), phage proteins, Stx2, FlgM and FlgD, a dienelactone hydrolase, CheW and CheY, and the SPATE protease EspP. For clade 6 strain, a high overexpression of phage proteins was detected, mostly from Stx2 encoding phage, including Stx2, flagellin and the protease TagA, EDL933_p0016, dienelactone hydrolase, and Haemolysin A, amongst others with unknown function. Some of these proteins were analyzed by RT-qPCR to corroborate the proteomic data. Clade 6 and clade 8 strains showed enhanced transcription of 10 out of 12 genes compared to EDL933. These results may provide new insights in E. coli O157:H7 mechanisms of pathogenesis.
Inst. de Biotecnología
Fil: Amigo, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Qi, Zhang. Guangdong ‘Academy of Agricultural Sciences. AGRO-Biological Gene Research Center; China
Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Qunjie, Zhang. Guangdong ‘Academy of Agricultural Sciences. AGRO-Biological Gene Research Center; China
Fil: Marques da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Zhongjian, Chen. Guangdong ‘Academy of Agricultural Sciences. AGRO-Biological Gene Research Center; China
Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Jinlong, Bei. Guangdong ‘Academy of Agricultural Sciences. AGRO-Biological Gene Research Center; China
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
PLoS ONE 11 (11) : e016688 (2016)
Materia
Genética
Enfermedades de los Animales
Escherichia Coli
Genetics
Animal Diseases
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1115
Acceder
id INTADig_676ebaf969238571a889a5ad4c8e17bd
oai_identifier_str oai:localhost:20.500.12123/1115
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Overexpressed proteins in hypervirulent clade 8 and clade 6 strains of Escherichia coli O157:H7 compared to E. coli O157:H7 EDL933 clade 3 strainAmigo, NataliaQi, ZhangAmadio, ArielQunjie, ZhangMarques da Silva, WandersonBaiyuan, CuiZhongjian, ChenLarzabal, MarianoCataldi, Angel AdrianJinlong, BeiGenéticaEnfermedades de los AnimalesEscherichia ColiGeneticsAnimal DiseasesEscherichia coli O157:H7 is responsible for severe diarrhea and hemolytic uremic syndrome (HUS), and predominantly affects children under 5 years. The major virulence traits are Shiga toxins, necessary to develop HUS and the Type III Secretion System (T3SS) through which bacteria translocate effector proteins directly into the host cell. By SNPs typing, E. coli O157:H7 was separated into nine different clades. Clade 8 and clade 6 strains were more frequently associated with severe disease and HUS. In this study, we aimed to identify differentially expressed proteins in two strains of E. coli O157:H7 (clade 8 and clade 6), obtained from cattle and compared them with the well characterized reference EDL933 strain (clade 3). Clade 8 and clade 6 strains show enhanced pathogenicity in a mouse model and virulence-related properties. Proteins were extracted and analyzed using the TMT-6plex labeling strategy associated with two dimensional liquid chromatography and mass spectrometry in tandem. We detected 2241 proteins in the cell extract and 1787 proteins in the culture supernatants. Attention was focused on the proteins related to virulence, overexpressed in clade 6 and 8 strains compared to EDL933 strain. The proteins relevant overexpressed in clade 8 strain were the curli protein CsgC, a transcriptional activator (PchE), phage proteins, Stx2, FlgM and FlgD, a dienelactone hydrolase, CheW and CheY, and the SPATE protease EspP. For clade 6 strain, a high overexpression of phage proteins was detected, mostly from Stx2 encoding phage, including Stx2, flagellin and the protease TagA, EDL933_p0016, dienelactone hydrolase, and Haemolysin A, amongst others with unknown function. Some of these proteins were analyzed by RT-qPCR to corroborate the proteomic data. Clade 6 and clade 8 strains showed enhanced transcription of 10 out of 12 genes compared to EDL933. These results may provide new insights in E. coli O157:H7 mechanisms of pathogenesis.Inst. de BiotecnologíaFil: Amigo, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Qi, Zhang. Guangdong ‘Academy of Agricultural Sciences. AGRO-Biological Gene Research Center; ChinaFil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Qunjie, Zhang. Guangdong ‘Academy of Agricultural Sciences. AGRO-Biological Gene Research Center; ChinaFil: Marques da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Zhongjian, Chen. Guangdong ‘Academy of Agricultural Sciences. AGRO-Biological Gene Research Center; ChinaFil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Jinlong, Bei. Guangdong ‘Academy of Agricultural Sciences. AGRO-Biological Gene Research Center; ChinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina2017-09-04T13:41:40Z2017-09-04T13:41:40Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1115http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0166883Amigo N, Zhang Q, Amadio A, Zhang Q, Silva WM, Cui B, et al. (2016) Overexpressed Proteins in Hypervirulent Clade 8 and Clade 6 Strains of Escherichia coli O157:H7 Compared to E. coli O157:H7 EDL933 Clade 3 Strain. PLoS ONE 11(11): e01668831932-6203 (Online)https://doi.org/10.1371/journal.pone.0166883PLoS ONE 11 (11) : e016688 (2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:28:59Zoai:localhost:20.500.12123/1115instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:00.344INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Overexpressed proteins in hypervirulent clade 8 and clade 6 strains of Escherichia coli O157:H7 compared to E. coli O157:H7 EDL933 clade 3 strain
title Overexpressed proteins in hypervirulent clade 8 and clade 6 strains of Escherichia coli O157:H7 compared to E. coli O157:H7 EDL933 clade 3 strain
spellingShingle Overexpressed proteins in hypervirulent clade 8 and clade 6 strains of Escherichia coli O157:H7 compared to E. coli O157:H7 EDL933 clade 3 strain
Amigo, Natalia
Genética
Enfermedades de los Animales
Escherichia Coli
Genetics
Animal Diseases
title_short Overexpressed proteins in hypervirulent clade 8 and clade 6 strains of Escherichia coli O157:H7 compared to E. coli O157:H7 EDL933 clade 3 strain
title_full Overexpressed proteins in hypervirulent clade 8 and clade 6 strains of Escherichia coli O157:H7 compared to E. coli O157:H7 EDL933 clade 3 strain
title_fullStr Overexpressed proteins in hypervirulent clade 8 and clade 6 strains of Escherichia coli O157:H7 compared to E. coli O157:H7 EDL933 clade 3 strain
title_full_unstemmed Overexpressed proteins in hypervirulent clade 8 and clade 6 strains of Escherichia coli O157:H7 compared to E. coli O157:H7 EDL933 clade 3 strain
title_sort Overexpressed proteins in hypervirulent clade 8 and clade 6 strains of Escherichia coli O157:H7 compared to E. coli O157:H7 EDL933 clade 3 strain
dc.creator.none.fl_str_mv Amigo, Natalia
Qi, Zhang
Amadio, Ariel
Qunjie, Zhang
Marques da Silva, Wanderson
Baiyuan, Cui
Zhongjian, Chen
Larzabal, Mariano
Cataldi, Angel Adrian
Jinlong, Bei
author Amigo, Natalia
author_facet Amigo, Natalia
Qi, Zhang
Amadio, Ariel
Qunjie, Zhang
Marques da Silva, Wanderson
Baiyuan, Cui
Zhongjian, Chen
Larzabal, Mariano
Cataldi, Angel Adrian
Jinlong, Bei
author_role author
author2 Qi, Zhang
Amadio, Ariel
Qunjie, Zhang
Marques da Silva, Wanderson
Baiyuan, Cui
Zhongjian, Chen
Larzabal, Mariano
Cataldi, Angel Adrian
Jinlong, Bei
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Genética
Enfermedades de los Animales
Escherichia Coli
Genetics
Animal Diseases
topic Genética
Enfermedades de los Animales
Escherichia Coli
Genetics
Animal Diseases
dc.description.none.fl_txt_mv Escherichia coli O157:H7 is responsible for severe diarrhea and hemolytic uremic syndrome (HUS), and predominantly affects children under 5 years. The major virulence traits are Shiga toxins, necessary to develop HUS and the Type III Secretion System (T3SS) through which bacteria translocate effector proteins directly into the host cell. By SNPs typing, E. coli O157:H7 was separated into nine different clades. Clade 8 and clade 6 strains were more frequently associated with severe disease and HUS. In this study, we aimed to identify differentially expressed proteins in two strains of E. coli O157:H7 (clade 8 and clade 6), obtained from cattle and compared them with the well characterized reference EDL933 strain (clade 3). Clade 8 and clade 6 strains show enhanced pathogenicity in a mouse model and virulence-related properties. Proteins were extracted and analyzed using the TMT-6plex labeling strategy associated with two dimensional liquid chromatography and mass spectrometry in tandem. We detected 2241 proteins in the cell extract and 1787 proteins in the culture supernatants. Attention was focused on the proteins related to virulence, overexpressed in clade 6 and 8 strains compared to EDL933 strain. The proteins relevant overexpressed in clade 8 strain were the curli protein CsgC, a transcriptional activator (PchE), phage proteins, Stx2, FlgM and FlgD, a dienelactone hydrolase, CheW and CheY, and the SPATE protease EspP. For clade 6 strain, a high overexpression of phage proteins was detected, mostly from Stx2 encoding phage, including Stx2, flagellin and the protease TagA, EDL933_p0016, dienelactone hydrolase, and Haemolysin A, amongst others with unknown function. Some of these proteins were analyzed by RT-qPCR to corroborate the proteomic data. Clade 6 and clade 8 strains showed enhanced transcription of 10 out of 12 genes compared to EDL933. These results may provide new insights in E. coli O157:H7 mechanisms of pathogenesis.
Inst. de Biotecnología
Fil: Amigo, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Qi, Zhang. Guangdong ‘Academy of Agricultural Sciences. AGRO-Biological Gene Research Center; China
Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Qunjie, Zhang. Guangdong ‘Academy of Agricultural Sciences. AGRO-Biological Gene Research Center; China
Fil: Marques da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Zhongjian, Chen. Guangdong ‘Academy of Agricultural Sciences. AGRO-Biological Gene Research Center; China
Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Jinlong, Bei. Guangdong ‘Academy of Agricultural Sciences. AGRO-Biological Gene Research Center; China
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description Escherichia coli O157:H7 is responsible for severe diarrhea and hemolytic uremic syndrome (HUS), and predominantly affects children under 5 years. The major virulence traits are Shiga toxins, necessary to develop HUS and the Type III Secretion System (T3SS) through which bacteria translocate effector proteins directly into the host cell. By SNPs typing, E. coli O157:H7 was separated into nine different clades. Clade 8 and clade 6 strains were more frequently associated with severe disease and HUS. In this study, we aimed to identify differentially expressed proteins in two strains of E. coli O157:H7 (clade 8 and clade 6), obtained from cattle and compared them with the well characterized reference EDL933 strain (clade 3). Clade 8 and clade 6 strains show enhanced pathogenicity in a mouse model and virulence-related properties. Proteins were extracted and analyzed using the TMT-6plex labeling strategy associated with two dimensional liquid chromatography and mass spectrometry in tandem. We detected 2241 proteins in the cell extract and 1787 proteins in the culture supernatants. Attention was focused on the proteins related to virulence, overexpressed in clade 6 and 8 strains compared to EDL933 strain. The proteins relevant overexpressed in clade 8 strain were the curli protein CsgC, a transcriptional activator (PchE), phage proteins, Stx2, FlgM and FlgD, a dienelactone hydrolase, CheW and CheY, and the SPATE protease EspP. For clade 6 strain, a high overexpression of phage proteins was detected, mostly from Stx2 encoding phage, including Stx2, flagellin and the protease TagA, EDL933_p0016, dienelactone hydrolase, and Haemolysin A, amongst others with unknown function. Some of these proteins were analyzed by RT-qPCR to corroborate the proteomic data. Clade 6 and clade 8 strains showed enhanced transcription of 10 out of 12 genes compared to EDL933. These results may provide new insights in E. coli O157:H7 mechanisms of pathogenesis.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-09-04T13:41:40Z
2017-09-04T13:41:40Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1115
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0166883
Amigo N, Zhang Q, Amadio A, Zhang Q, Silva WM, Cui B, et al. (2016) Overexpressed Proteins in Hypervirulent Clade 8 and Clade 6 Strains of Escherichia coli O157:H7 Compared to E. coli O157:H7 EDL933 Clade 3 Strain. PLoS ONE 11(11): e0166883
1932-6203 (Online)
https://doi.org/10.1371/journal.pone.0166883
url http://hdl.handle.net/20.500.12123/1115
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0166883
https://doi.org/10.1371/journal.pone.0166883
identifier_str_mv Amigo N, Zhang Q, Amadio A, Zhang Q, Silva WM, Cui B, et al. (2016) Overexpressed Proteins in Hypervirulent Clade 8 and Clade 6 Strains of Escherichia coli O157:H7 Compared to E. coli O157:H7 EDL933 Clade 3 Strain. PLoS ONE 11(11): e0166883
1932-6203 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv PLoS ONE 11 (11) : e016688 (2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1846143494996885504
score 12.712165

Publicaciones similares