Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice

Autores
Bonell, Martha Lucrecia; Arguissain, Gustavo Gabriel; Crepy, Maria Andrea; Fernandez, Paula Del Carmen; Heinz, Ruth Amelia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina.
EEA Concepción del Uruguay
Fil: Bonell, Martha Lucrecia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Arguissain, Gustavo Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Crepy, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Euphytica 216 : article number: 128 (2020)
Materia
Arroz
Tolerancia al Frío
Fenotipos
Loci de Rasgos Cuantitativos
Argentina
Rice
Cold Tolerance
Phenotypes
Quantitative Trait Loci
QTL (quantitative trait loci)
Genes Candidatos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7629
Acceder
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oai_identifier_str oai:localhost:20.500.12123/7629
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate riceBonell, Martha LucreciaArguissain, Gustavo GabrielCrepy, Maria AndreaFernandez, Paula Del CarmenHeinz, Ruth AmeliaArrozTolerancia al FríoFenotiposLoci de Rasgos CuantitativosArgentinaRiceCold TolerancePhenotypesQuantitative Trait LociQTL (quantitative trait loci)Genes CandidatosIn Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina.EEA Concepción del UruguayFil: Bonell, Martha Lucrecia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Arguissain, Gustavo Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Crepy, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2020-07-28T17:15:57Z2020-07-28T17:15:57Z2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7629https://link.springer.com/article/10.1007/s10681-020-02661-30014-23361573-5060https://doi.org/10.1007/s10681-020-02661-3Euphytica 216 : article number: 128 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:59Zoai:localhost:20.500.12123/7629instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:00.0INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
title Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
spellingShingle Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
Bonell, Martha Lucrecia
Arroz
Tolerancia al Frío
Fenotipos
Loci de Rasgos Cuantitativos
Argentina
Rice
Cold Tolerance
Phenotypes
Quantitative Trait Loci
QTL (quantitative trait loci)
Genes Candidatos
title_short Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
title_full Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
title_fullStr Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
title_full_unstemmed Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
title_sort Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
dc.creator.none.fl_str_mv Bonell, Martha Lucrecia
Arguissain, Gustavo Gabriel
Crepy, Maria Andrea
Fernandez, Paula Del Carmen
Heinz, Ruth Amelia
author Bonell, Martha Lucrecia
author_facet Bonell, Martha Lucrecia
Arguissain, Gustavo Gabriel
Crepy, Maria Andrea
Fernandez, Paula Del Carmen
Heinz, Ruth Amelia
author_role author
author2 Arguissain, Gustavo Gabriel
Crepy, Maria Andrea
Fernandez, Paula Del Carmen
Heinz, Ruth Amelia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Arroz
Tolerancia al Frío
Fenotipos
Loci de Rasgos Cuantitativos
Argentina
Rice
Cold Tolerance
Phenotypes
Quantitative Trait Loci
QTL (quantitative trait loci)
Genes Candidatos
topic Arroz
Tolerancia al Frío
Fenotipos
Loci de Rasgos Cuantitativos
Argentina
Rice
Cold Tolerance
Phenotypes
Quantitative Trait Loci
QTL (quantitative trait loci)
Genes Candidatos
dc.description.none.fl_txt_mv In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina.
EEA Concepción del Uruguay
Fil: Bonell, Martha Lucrecia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Arguissain, Gustavo Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Crepy, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina.
publishDate 2020
dc.date.none.fl_str_mv 2020-07-28T17:15:57Z
2020-07-28T17:15:57Z
2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7629
https://link.springer.com/article/10.1007/s10681-020-02661-3
0014-2336
1573-5060
https://doi.org/10.1007/s10681-020-02661-3
url http://hdl.handle.net/20.500.12123/7629
https://link.springer.com/article/10.1007/s10681-020-02661-3
https://doi.org/10.1007/s10681-020-02661-3
identifier_str_mv 0014-2336
1573-5060
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Euphytica 216 : article number: 128 (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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