Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
- Autores
- Bonell, Martha Lucrecia; Arguissain, Gustavo Gabriel; Crepy, Maria Andrea; Fernandez, Paula Del Carmen; Heinz, Ruth Amelia
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina.
EEA Concepción del Uruguay
Fil: Bonell, Martha Lucrecia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Arguissain, Gustavo Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Crepy, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Euphytica 216 : article number: 128 (2020)
- Materia
-
Arroz
Tolerancia al Frío
Fenotipos
Loci de Rasgos Cuantitativos
Argentina
Rice
Cold Tolerance
Phenotypes
Quantitative Trait Loci
QTL (quantitative trait loci)
Genes Candidatos - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/7629
Ver los metadatos del registro completo
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Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate riceBonell, Martha LucreciaArguissain, Gustavo GabrielCrepy, Maria AndreaFernandez, Paula Del CarmenHeinz, Ruth AmeliaArrozTolerancia al FríoFenotiposLoci de Rasgos CuantitativosArgentinaRiceCold TolerancePhenotypesQuantitative Trait LociQTL (quantitative trait loci)Genes CandidatosIn Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina.EEA Concepción del UruguayFil: Bonell, Martha Lucrecia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Arguissain, Gustavo Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Crepy, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2020-07-28T17:15:57Z2020-07-28T17:15:57Z2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7629https://link.springer.com/article/10.1007/s10681-020-02661-30014-23361573-5060https://doi.org/10.1007/s10681-020-02661-3Euphytica 216 : article number: 128 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:17:20Zoai:localhost:20.500.12123/7629instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:20.896INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
| title |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
| spellingShingle |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice Bonell, Martha Lucrecia Arroz Tolerancia al Frío Fenotipos Loci de Rasgos Cuantitativos Argentina Rice Cold Tolerance Phenotypes Quantitative Trait Loci QTL (quantitative trait loci) Genes Candidatos |
| title_short |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
| title_full |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
| title_fullStr |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
| title_full_unstemmed |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
| title_sort |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
| dc.creator.none.fl_str_mv |
Bonell, Martha Lucrecia Arguissain, Gustavo Gabriel Crepy, Maria Andrea Fernandez, Paula Del Carmen Heinz, Ruth Amelia |
| author |
Bonell, Martha Lucrecia |
| author_facet |
Bonell, Martha Lucrecia Arguissain, Gustavo Gabriel Crepy, Maria Andrea Fernandez, Paula Del Carmen Heinz, Ruth Amelia |
| author_role |
author |
| author2 |
Arguissain, Gustavo Gabriel Crepy, Maria Andrea Fernandez, Paula Del Carmen Heinz, Ruth Amelia |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
Arroz Tolerancia al Frío Fenotipos Loci de Rasgos Cuantitativos Argentina Rice Cold Tolerance Phenotypes Quantitative Trait Loci QTL (quantitative trait loci) Genes Candidatos |
| topic |
Arroz Tolerancia al Frío Fenotipos Loci de Rasgos Cuantitativos Argentina Rice Cold Tolerance Phenotypes Quantitative Trait Loci QTL (quantitative trait loci) Genes Candidatos |
| dc.description.none.fl_txt_mv |
In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina. EEA Concepción del Uruguay Fil: Bonell, Martha Lucrecia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Arguissain, Gustavo Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Crepy, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| description |
In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-07-28T17:15:57Z 2020-07-28T17:15:57Z 2020-07 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/20.500.12123/7629 https://link.springer.com/article/10.1007/s10681-020-02661-3 0014-2336 1573-5060 https://doi.org/10.1007/s10681-020-02661-3 |
| url |
http://hdl.handle.net/20.500.12123/7629 https://link.springer.com/article/10.1007/s10681-020-02661-3 https://doi.org/10.1007/s10681-020-02661-3 |
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0014-2336 1573-5060 |
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eng |
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eng |
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application/pdf |
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Springer |
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Springer |
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Euphytica 216 : article number: 128 (2020) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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