Evaluating the impact of the biocontrol agent Trichoderma harzianum ITEM 3636 on indigenous microbial communities from field soils

Autores
Ganuza, Mauricio Roque; Pastor, Nicolás A.; Boccolini, Monica Fabiola; Erazo, Jessica Gabriela; Palacios, S.; Oddino, Claudio; Reynoso, María Marta; Rovera, Marisa; Torres, Adriana Mabel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Aim: to investigate the impact of inoculating peanut seeds with the biocontrol agent Trichoderma harzianum ITEM 3636 on the structure of bacterial and fungal communities from agricultural soils. Methods and Results: PCR‐Denaturing gradient gel electrophoresis (PCR‐DGGE) and next‐generation sequencing (NGS) of amplicons (or marker gene amplification metagenomics) were performed to investigate potential changes in the structure of microbial communities from fields located in a peanut‐producing area in the province of Córdoba, Argentina. Fields had history of peanut smut (caused by Thecaphora frezii) incidence. The Shannon indexes (H’), which estimate diversity, obtained from the PCR‐DGGE assays did not show significant differences neither for bacterial nor for fungal communities between control and inoculation treatments. On the other hand, the number of Operational Taxonomic Units (OTUs) obtained after NGS was similar between all the analyzed samples. Moreover, results of alpha and beta diversity showed that there were no significant variations between the relative abundances of the most representative bacterial and fungal phyla and genera, in both fields. Conclusions: T. harzianum ITEM 3636 decreases the incidence and severity of agriculturally relevant diseases without causing significant changes in the microbial communities of agricultural soils.
EEA Marcos Juárez
Fil: Ganuza, Mauricio Roque. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Pastor, Nicolás A. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Boccolini, Monica Fabiola. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Erazo, Jessica Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Palacios, S. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Oddino, C. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Reynoso, María Marta. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Rovera, Marisa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Torres, Adriana Mabel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fuente
Journal of Applied Microbiology First published: 01 November 2018
Materia
Trichoderma harzianum
Hongos
Suelo
Control Biológico
PCR
Cacahuete
Fungi
Soil
Biological Control
Groundnuts
Córdoba
Maní
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3936
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oai_identifier_str oai:localhost:20.500.12123/3936
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network_name_str INTA Digital (INTA)
spelling Evaluating the impact of the biocontrol agent Trichoderma harzianum ITEM 3636 on indigenous microbial communities from field soilsGanuza, Mauricio RoquePastor, Nicolás A.Boccolini, Monica FabiolaErazo, Jessica GabrielaPalacios, S.Oddino, ClaudioReynoso, María MartaRovera, MarisaTorres, Adriana MabelTrichoderma harzianumHongosSueloControl BiológicoPCRCacahueteFungiSoilBiological ControlGroundnutsCórdobaManíAim: to investigate the impact of inoculating peanut seeds with the biocontrol agent Trichoderma harzianum ITEM 3636 on the structure of bacterial and fungal communities from agricultural soils. Methods and Results: PCR‐Denaturing gradient gel electrophoresis (PCR‐DGGE) and next‐generation sequencing (NGS) of amplicons (or marker gene amplification metagenomics) were performed to investigate potential changes in the structure of microbial communities from fields located in a peanut‐producing area in the province of Córdoba, Argentina. Fields had history of peanut smut (caused by Thecaphora frezii) incidence. The Shannon indexes (H’), which estimate diversity, obtained from the PCR‐DGGE assays did not show significant differences neither for bacterial nor for fungal communities between control and inoculation treatments. On the other hand, the number of Operational Taxonomic Units (OTUs) obtained after NGS was similar between all the analyzed samples. Moreover, results of alpha and beta diversity showed that there were no significant variations between the relative abundances of the most representative bacterial and fungal phyla and genera, in both fields. Conclusions: T. harzianum ITEM 3636 decreases the incidence and severity of agriculturally relevant diseases without causing significant changes in the microbial communities of agricultural soils.EEA Marcos JuárezFil: Ganuza, Mauricio Roque. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Pastor, Nicolás A. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Boccolini, Monica Fabiola. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Erazo, Jessica Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Palacios, S. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Oddino, C. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Reynoso, María Marta. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Rovera, Marisa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Torres, Adriana Mabel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaWileyinfo:eu-repo/date/embargoEnd/2019-11-212018-11-21T13:28:05Z2018-11-21T13:28:05Z2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3936https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.141471364-50721365-2672https://doi.org/10.1111/jam.14147Journal of Applied Microbiology First published: 01 November 2018reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:42Zoai:localhost:20.500.12123/3936instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:42.655INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Evaluating the impact of the biocontrol agent Trichoderma harzianum ITEM 3636 on indigenous microbial communities from field soils
title Evaluating the impact of the biocontrol agent Trichoderma harzianum ITEM 3636 on indigenous microbial communities from field soils
spellingShingle Evaluating the impact of the biocontrol agent Trichoderma harzianum ITEM 3636 on indigenous microbial communities from field soils
Ganuza, Mauricio Roque
Trichoderma harzianum
Hongos
Suelo
Control Biológico
PCR
Cacahuete
Fungi
Soil
Biological Control
Groundnuts
Córdoba
Maní
title_short Evaluating the impact of the biocontrol agent Trichoderma harzianum ITEM 3636 on indigenous microbial communities from field soils
title_full Evaluating the impact of the biocontrol agent Trichoderma harzianum ITEM 3636 on indigenous microbial communities from field soils
title_fullStr Evaluating the impact of the biocontrol agent Trichoderma harzianum ITEM 3636 on indigenous microbial communities from field soils
title_full_unstemmed Evaluating the impact of the biocontrol agent Trichoderma harzianum ITEM 3636 on indigenous microbial communities from field soils
title_sort Evaluating the impact of the biocontrol agent Trichoderma harzianum ITEM 3636 on indigenous microbial communities from field soils
dc.creator.none.fl_str_mv Ganuza, Mauricio Roque
Pastor, Nicolás A.
Boccolini, Monica Fabiola
Erazo, Jessica Gabriela
Palacios, S.
Oddino, Claudio
Reynoso, María Marta
Rovera, Marisa
Torres, Adriana Mabel
author Ganuza, Mauricio Roque
author_facet Ganuza, Mauricio Roque
Pastor, Nicolás A.
Boccolini, Monica Fabiola
Erazo, Jessica Gabriela
Palacios, S.
Oddino, Claudio
Reynoso, María Marta
Rovera, Marisa
Torres, Adriana Mabel
author_role author
author2 Pastor, Nicolás A.
Boccolini, Monica Fabiola
Erazo, Jessica Gabriela
Palacios, S.
Oddino, Claudio
Reynoso, María Marta
Rovera, Marisa
Torres, Adriana Mabel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trichoderma harzianum
Hongos
Suelo
Control Biológico
PCR
Cacahuete
Fungi
Soil
Biological Control
Groundnuts
Córdoba
Maní
topic Trichoderma harzianum
Hongos
Suelo
Control Biológico
PCR
Cacahuete
Fungi
Soil
Biological Control
Groundnuts
Córdoba
Maní
dc.description.none.fl_txt_mv Aim: to investigate the impact of inoculating peanut seeds with the biocontrol agent Trichoderma harzianum ITEM 3636 on the structure of bacterial and fungal communities from agricultural soils. Methods and Results: PCR‐Denaturing gradient gel electrophoresis (PCR‐DGGE) and next‐generation sequencing (NGS) of amplicons (or marker gene amplification metagenomics) were performed to investigate potential changes in the structure of microbial communities from fields located in a peanut‐producing area in the province of Córdoba, Argentina. Fields had history of peanut smut (caused by Thecaphora frezii) incidence. The Shannon indexes (H’), which estimate diversity, obtained from the PCR‐DGGE assays did not show significant differences neither for bacterial nor for fungal communities between control and inoculation treatments. On the other hand, the number of Operational Taxonomic Units (OTUs) obtained after NGS was similar between all the analyzed samples. Moreover, results of alpha and beta diversity showed that there were no significant variations between the relative abundances of the most representative bacterial and fungal phyla and genera, in both fields. Conclusions: T. harzianum ITEM 3636 decreases the incidence and severity of agriculturally relevant diseases without causing significant changes in the microbial communities of agricultural soils.
EEA Marcos Juárez
Fil: Ganuza, Mauricio Roque. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Pastor, Nicolás A. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Boccolini, Monica Fabiola. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Erazo, Jessica Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Palacios, S. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Oddino, C. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Reynoso, María Marta. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Rovera, Marisa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Torres, Adriana Mabel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico‐Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
description Aim: to investigate the impact of inoculating peanut seeds with the biocontrol agent Trichoderma harzianum ITEM 3636 on the structure of bacterial and fungal communities from agricultural soils. Methods and Results: PCR‐Denaturing gradient gel electrophoresis (PCR‐DGGE) and next‐generation sequencing (NGS) of amplicons (or marker gene amplification metagenomics) were performed to investigate potential changes in the structure of microbial communities from fields located in a peanut‐producing area in the province of Córdoba, Argentina. Fields had history of peanut smut (caused by Thecaphora frezii) incidence. The Shannon indexes (H’), which estimate diversity, obtained from the PCR‐DGGE assays did not show significant differences neither for bacterial nor for fungal communities between control and inoculation treatments. On the other hand, the number of Operational Taxonomic Units (OTUs) obtained after NGS was similar between all the analyzed samples. Moreover, results of alpha and beta diversity showed that there were no significant variations between the relative abundances of the most representative bacterial and fungal phyla and genera, in both fields. Conclusions: T. harzianum ITEM 3636 decreases the incidence and severity of agriculturally relevant diseases without causing significant changes in the microbial communities of agricultural soils.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-21T13:28:05Z
2018-11-21T13:28:05Z
2018-11
info:eu-repo/date/embargoEnd/2019-11-21
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3936
https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.14147
1364-5072
1365-2672
https://doi.org/10.1111/jam.14147
url http://hdl.handle.net/20.500.12123/3936
https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.14147
https://doi.org/10.1111/jam.14147
identifier_str_mv 1364-5072
1365-2672
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Journal of Applied Microbiology First published: 01 November 2018
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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