Seropositivity to Sarcocystis infection of llamas correlates with breeding practices

Autores
Romero, Sandra Raquel; Carletti, Tamara; Decker Franco, Cecilia; Moré, Gastón; Schnittger, Leonhard; Florin-Christensen, Mónica
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Production of llama (Lama glama) meat in rural communities of the Andean regions is largely affected by Sarcocystis spp. infection. Macroscopic cysts develop in muscles as a consequence of S. aucheniae parasitism, often resulting in meat downgrade or condemnation. Llama meat production is informal in Argentina but has broad perspectives for improvement, and would significantly benefit from the development of standardized control methodologies. This work analyzes whether the presence of anti-Sarcocystis spp. antibodies in llamas is influenced by factors such as geographic region and/or herd management practices. To this aim, an indirect ELISA was set up based on a ~ 23 kDa soluble immunogenic protein fraction (Sa23), isolated from S. aucheniae macrocysts (Sa23-iELISA). Serum samples (n = 507) were collected from llamas bred under three different conditions: (i) with no sanitation controls and in the presence of pastoral dogs by small producers of different localities of the Argentine Puna (Group I, n = 237); (ii) with sanitation controls and no pastoral dogs, in fenced fields of an experimental agricultural station in the Argentine Puna (Group II, n = 167); and (iii) with sanitation controls and no pastoral dogs in fenced fields of farms of the humid Pampas (Group III, n = 103). Results of the Sa23-iELISA were expressed as percentages of positivity with respect to a reference Sarcocystis-positive serum. Notably, the percentage of sera that fell above the cut-off (31.5% positivity) in group (i) was significantly higher (p < 0.001) than those of groups (ii) and (iii) (50% vs 23% and 26%, respectively). These results indicate that herd management practices constitute a critical risk factor for sarcocystiosis in llamas. Differences in these practices include feeding of dogs with raw Sarcocystis-infected llama meat, with the consequent maintenance of the parasite life cycle by the contamination of pastures and water with fecal-derived infective oocysts/sporocysts. Additionally, the itinerancy of llama herds in search for pastures and water sources possibly exposes animals to a higher number of infective foci. On the other hand, percentages of seropositive llamas kept under controlled conditions in the Puna or the humid Pampas were not significantly different, suggesting that climate, altitude, and/or pasture characteristics do not influence Sarcocystis-infection. Male gender and older age of llamas were found to be propensity factors for sarcocystiosis in llamas bred in La Puna under controlled conditions. Availability of diagnostic tools, as well as increased knowledge on the parasite and its epidemiology, will allow the design of control strategies for SAC sarcocystiosis.
IPAF NOA
Fil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación y Desarrollo Tecnológico para la Agricultura Familiar Región NOA; Argentina
Fil: Carletti, Tamara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Veterinary parasitology: regional studies and reports 10 : 65-70. (December 2017)
Materia
Sarcocystis
Llama
Mejoramiento Animal
Infección
Llamas
Animal Breeding
Infection
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1950

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network_name_str INTA Digital (INTA)
spelling Seropositivity to Sarcocystis infection of llamas correlates with breeding practicesRomero, Sandra RaquelCarletti, TamaraDecker Franco, CeciliaMoré, GastónSchnittger, LeonhardFlorin-Christensen, MónicaSarcocystisLlamaMejoramiento AnimalInfecciónLlamasAnimal BreedingInfectionProduction of llama (Lama glama) meat in rural communities of the Andean regions is largely affected by Sarcocystis spp. infection. Macroscopic cysts develop in muscles as a consequence of S. aucheniae parasitism, often resulting in meat downgrade or condemnation. Llama meat production is informal in Argentina but has broad perspectives for improvement, and would significantly benefit from the development of standardized control methodologies. This work analyzes whether the presence of anti-Sarcocystis spp. antibodies in llamas is influenced by factors such as geographic region and/or herd management practices. To this aim, an indirect ELISA was set up based on a ~ 23 kDa soluble immunogenic protein fraction (Sa23), isolated from S. aucheniae macrocysts (Sa23-iELISA). Serum samples (n = 507) were collected from llamas bred under three different conditions: (i) with no sanitation controls and in the presence of pastoral dogs by small producers of different localities of the Argentine Puna (Group I, n = 237); (ii) with sanitation controls and no pastoral dogs, in fenced fields of an experimental agricultural station in the Argentine Puna (Group II, n = 167); and (iii) with sanitation controls and no pastoral dogs in fenced fields of farms of the humid Pampas (Group III, n = 103). Results of the Sa23-iELISA were expressed as percentages of positivity with respect to a reference Sarcocystis-positive serum. Notably, the percentage of sera that fell above the cut-off (31.5% positivity) in group (i) was significantly higher (p < 0.001) than those of groups (ii) and (iii) (50% vs 23% and 26%, respectively). These results indicate that herd management practices constitute a critical risk factor for sarcocystiosis in llamas. Differences in these practices include feeding of dogs with raw Sarcocystis-infected llama meat, with the consequent maintenance of the parasite life cycle by the contamination of pastures and water with fecal-derived infective oocysts/sporocysts. Additionally, the itinerancy of llama herds in search for pastures and water sources possibly exposes animals to a higher number of infective foci. On the other hand, percentages of seropositive llamas kept under controlled conditions in the Puna or the humid Pampas were not significantly different, suggesting that climate, altitude, and/or pasture characteristics do not influence Sarcocystis-infection. Male gender and older age of llamas were found to be propensity factors for sarcocystiosis in llamas bred in La Puna under controlled conditions. Availability of diagnostic tools, as well as increased knowledge on the parasite and its epidemiology, will allow the design of control strategies for SAC sarcocystiosis.IPAF NOAFil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación y Desarrollo Tecnológico para la Agricultura Familiar Región NOA; ArgentinaFil: Carletti, Tamara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2018-03-02T18:20:32Z2018-03-02T18:20:32Z2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1950https://www.sciencedirect.com/science/article/pii/S24059390163024282405-9390https://doi.org/10.1016/j.vprsr.2017.08.006Veterinary parasitology: regional studies and reports 10 : 65-70. (December 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:15Zoai:localhost:20.500.12123/1950instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:15.732INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Seropositivity to Sarcocystis infection of llamas correlates with breeding practices
title Seropositivity to Sarcocystis infection of llamas correlates with breeding practices
spellingShingle Seropositivity to Sarcocystis infection of llamas correlates with breeding practices
Romero, Sandra Raquel
Sarcocystis
Llama
Mejoramiento Animal
Infección
Llamas
Animal Breeding
Infection
title_short Seropositivity to Sarcocystis infection of llamas correlates with breeding practices
title_full Seropositivity to Sarcocystis infection of llamas correlates with breeding practices
title_fullStr Seropositivity to Sarcocystis infection of llamas correlates with breeding practices
title_full_unstemmed Seropositivity to Sarcocystis infection of llamas correlates with breeding practices
title_sort Seropositivity to Sarcocystis infection of llamas correlates with breeding practices
dc.creator.none.fl_str_mv Romero, Sandra Raquel
Carletti, Tamara
Decker Franco, Cecilia
Moré, Gastón
Schnittger, Leonhard
Florin-Christensen, Mónica
author Romero, Sandra Raquel
author_facet Romero, Sandra Raquel
Carletti, Tamara
Decker Franco, Cecilia
Moré, Gastón
Schnittger, Leonhard
Florin-Christensen, Mónica
author_role author
author2 Carletti, Tamara
Decker Franco, Cecilia
Moré, Gastón
Schnittger, Leonhard
Florin-Christensen, Mónica
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Sarcocystis
Llama
Mejoramiento Animal
Infección
Llamas
Animal Breeding
Infection
topic Sarcocystis
Llama
Mejoramiento Animal
Infección
Llamas
Animal Breeding
Infection
dc.description.none.fl_txt_mv Production of llama (Lama glama) meat in rural communities of the Andean regions is largely affected by Sarcocystis spp. infection. Macroscopic cysts develop in muscles as a consequence of S. aucheniae parasitism, often resulting in meat downgrade or condemnation. Llama meat production is informal in Argentina but has broad perspectives for improvement, and would significantly benefit from the development of standardized control methodologies. This work analyzes whether the presence of anti-Sarcocystis spp. antibodies in llamas is influenced by factors such as geographic region and/or herd management practices. To this aim, an indirect ELISA was set up based on a ~ 23 kDa soluble immunogenic protein fraction (Sa23), isolated from S. aucheniae macrocysts (Sa23-iELISA). Serum samples (n = 507) were collected from llamas bred under three different conditions: (i) with no sanitation controls and in the presence of pastoral dogs by small producers of different localities of the Argentine Puna (Group I, n = 237); (ii) with sanitation controls and no pastoral dogs, in fenced fields of an experimental agricultural station in the Argentine Puna (Group II, n = 167); and (iii) with sanitation controls and no pastoral dogs in fenced fields of farms of the humid Pampas (Group III, n = 103). Results of the Sa23-iELISA were expressed as percentages of positivity with respect to a reference Sarcocystis-positive serum. Notably, the percentage of sera that fell above the cut-off (31.5% positivity) in group (i) was significantly higher (p < 0.001) than those of groups (ii) and (iii) (50% vs 23% and 26%, respectively). These results indicate that herd management practices constitute a critical risk factor for sarcocystiosis in llamas. Differences in these practices include feeding of dogs with raw Sarcocystis-infected llama meat, with the consequent maintenance of the parasite life cycle by the contamination of pastures and water with fecal-derived infective oocysts/sporocysts. Additionally, the itinerancy of llama herds in search for pastures and water sources possibly exposes animals to a higher number of infective foci. On the other hand, percentages of seropositive llamas kept under controlled conditions in the Puna or the humid Pampas were not significantly different, suggesting that climate, altitude, and/or pasture characteristics do not influence Sarcocystis-infection. Male gender and older age of llamas were found to be propensity factors for sarcocystiosis in llamas bred in La Puna under controlled conditions. Availability of diagnostic tools, as well as increased knowledge on the parasite and its epidemiology, will allow the design of control strategies for SAC sarcocystiosis.
IPAF NOA
Fil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación y Desarrollo Tecnológico para la Agricultura Familiar Región NOA; Argentina
Fil: Carletti, Tamara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Production of llama (Lama glama) meat in rural communities of the Andean regions is largely affected by Sarcocystis spp. infection. Macroscopic cysts develop in muscles as a consequence of S. aucheniae parasitism, often resulting in meat downgrade or condemnation. Llama meat production is informal in Argentina but has broad perspectives for improvement, and would significantly benefit from the development of standardized control methodologies. This work analyzes whether the presence of anti-Sarcocystis spp. antibodies in llamas is influenced by factors such as geographic region and/or herd management practices. To this aim, an indirect ELISA was set up based on a ~ 23 kDa soluble immunogenic protein fraction (Sa23), isolated from S. aucheniae macrocysts (Sa23-iELISA). Serum samples (n = 507) were collected from llamas bred under three different conditions: (i) with no sanitation controls and in the presence of pastoral dogs by small producers of different localities of the Argentine Puna (Group I, n = 237); (ii) with sanitation controls and no pastoral dogs, in fenced fields of an experimental agricultural station in the Argentine Puna (Group II, n = 167); and (iii) with sanitation controls and no pastoral dogs in fenced fields of farms of the humid Pampas (Group III, n = 103). Results of the Sa23-iELISA were expressed as percentages of positivity with respect to a reference Sarcocystis-positive serum. Notably, the percentage of sera that fell above the cut-off (31.5% positivity) in group (i) was significantly higher (p < 0.001) than those of groups (ii) and (iii) (50% vs 23% and 26%, respectively). These results indicate that herd management practices constitute a critical risk factor for sarcocystiosis in llamas. Differences in these practices include feeding of dogs with raw Sarcocystis-infected llama meat, with the consequent maintenance of the parasite life cycle by the contamination of pastures and water with fecal-derived infective oocysts/sporocysts. Additionally, the itinerancy of llama herds in search for pastures and water sources possibly exposes animals to a higher number of infective foci. On the other hand, percentages of seropositive llamas kept under controlled conditions in the Puna or the humid Pampas were not significantly different, suggesting that climate, altitude, and/or pasture characteristics do not influence Sarcocystis-infection. Male gender and older age of llamas were found to be propensity factors for sarcocystiosis in llamas bred in La Puna under controlled conditions. Availability of diagnostic tools, as well as increased knowledge on the parasite and its epidemiology, will allow the design of control strategies for SAC sarcocystiosis.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
2018-03-02T18:20:32Z
2018-03-02T18:20:32Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1950
https://www.sciencedirect.com/science/article/pii/S2405939016302428
2405-9390
https://doi.org/10.1016/j.vprsr.2017.08.006
url http://hdl.handle.net/20.500.12123/1950
https://www.sciencedirect.com/science/article/pii/S2405939016302428
https://doi.org/10.1016/j.vprsr.2017.08.006
identifier_str_mv 2405-9390
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Veterinary parasitology: regional studies and reports 10 : 65-70. (December 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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