Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012

Autores
Olguin Perglione, Cecilia; Rimondi, Agustina; Gildea, Sarah; Miño, Samuel; Vissani, Maria Aldana; Carossino, Mariano; Cullinane, Ann; Barrandeguy, Maria Edith
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: In 2012, equine influenza (EI) virus was confirmed as the cause of outbreaks of respiratory disease in horses throughout South America. In Uruguay and Argentina, hundreds of vaccinated thoroughbred horses in training and racing facilities were clinically affected. Objective: To characterise the EI viruses detected during the outbreak in Uruguay and Argentina. Methods: Virus was detected in nasopharyngeal swabs by a pan-reactive influenza type A real-time RT-PCR. The nucleotide sequence of the HA1 gene was determined and analysed phylogenetically using mega 5 software. Amino acid sequences alignments were constructed and virus was antigenically characterised with specific ferret antisera. Paired serum samples were tested by haemagglutination inhibition and single radial haemolysis. Results: The diagnosis of EIV was confirmed by real-time RT-PCR, virus isolation and serological testing. The phylogenetic analysis of HA1 gene sequences of 18 EI viruses indicated that all of them belong to clade 1 of the Florida sublineage of the American lineage and are closely related to viruses isolated in the United States in 2012. The HA1 of viruses identified in horses in racing facilities in Maroñas, Uruguay, and in Palermo, Argentina, displayed 100% amino acid sequence identity and were identical to that of a virus isolated in Dubai in 2012, from vaccinated endurance horses recently imported from Uruguay. Conclusions: The surveillance data reported illustrate the international spread of EI viruses and support the recommendations of the OIE expert surveillance panel to include viruses of the Florida sublineage in vaccines.
Fil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rimondi, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Gildea, Sarah. The Irish Equine Centre. Virology Unit; Irlanda
Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Carossino, Mariano. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos
Fil: Cullinane, Ann. The Irish Equine Centre. Virology Unit; Irlanda
Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina
Fuente
Influenza and Other Respiratory Viruses 10 (1) : 37–46 (January 2016)
Materia
Equine Influenzavirus
Animal Diseases
Horses
Epidemiology
Virus de la Influenza Equina
Enfermedades de los Animales
Caballos
Epidemiología
América del Sur
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1222
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oai_identifier_str oai:localhost:20.500.12123/1222
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network_name_str INTA Digital (INTA)
spelling Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012Olguin Perglione, CeciliaRimondi, AgustinaGildea, SarahMiño, SamuelVissani, Maria AldanaCarossino, MarianoCullinane, AnnBarrandeguy, Maria EdithEquine InfluenzavirusAnimal DiseasesHorsesEpidemiologyVirus de la Influenza EquinaEnfermedades de los AnimalesCaballosEpidemiologíaAmérica del SurBackground: In 2012, equine influenza (EI) virus was confirmed as the cause of outbreaks of respiratory disease in horses throughout South America. In Uruguay and Argentina, hundreds of vaccinated thoroughbred horses in training and racing facilities were clinically affected. Objective: To characterise the EI viruses detected during the outbreak in Uruguay and Argentina. Methods: Virus was detected in nasopharyngeal swabs by a pan-reactive influenza type A real-time RT-PCR. The nucleotide sequence of the HA1 gene was determined and analysed phylogenetically using mega 5 software. Amino acid sequences alignments were constructed and virus was antigenically characterised with specific ferret antisera. Paired serum samples were tested by haemagglutination inhibition and single radial haemolysis. Results: The diagnosis of EIV was confirmed by real-time RT-PCR, virus isolation and serological testing. The phylogenetic analysis of HA1 gene sequences of 18 EI viruses indicated that all of them belong to clade 1 of the Florida sublineage of the American lineage and are closely related to viruses isolated in the United States in 2012. The HA1 of viruses identified in horses in racing facilities in Maroñas, Uruguay, and in Palermo, Argentina, displayed 100% amino acid sequence identity and were identical to that of a virus isolated in Dubai in 2012, from vaccinated endurance horses recently imported from Uruguay. Conclusions: The surveillance data reported illustrate the international spread of EI viruses and support the recommendations of the OIE expert surveillance panel to include viruses of the Florida sublineage in vaccines.Fil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Rimondi, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Gildea, Sarah. The Irish Equine Centre. Virology Unit; IrlandaFil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Carossino, Mariano. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados UnidosFil: Cullinane, Ann. The Irish Equine Centre. Virology Unit; IrlandaFil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina2017-09-14T14:53:57Z2017-09-14T14:53:57Z2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1222http://onlinelibrary.wiley.com/doi/10.1111/irv.12349/epdf1750-2659DOI: 10.1111/irv.12349Influenza and Other Respiratory Viruses 10 (1) : 37–46 (January 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaeng2012info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:10Zoai:localhost:20.500.12123/1222instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:10.918INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012
title Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012
spellingShingle Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012
Olguin Perglione, Cecilia
Equine Influenzavirus
Animal Diseases
Horses
Epidemiology
Virus de la Influenza Equina
Enfermedades de los Animales
Caballos
Epidemiología
América del Sur
title_short Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012
title_full Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012
title_fullStr Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012
title_full_unstemmed Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012
title_sort Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012
dc.creator.none.fl_str_mv Olguin Perglione, Cecilia
Rimondi, Agustina
Gildea, Sarah
Miño, Samuel
Vissani, Maria Aldana
Carossino, Mariano
Cullinane, Ann
Barrandeguy, Maria Edith
author Olguin Perglione, Cecilia
author_facet Olguin Perglione, Cecilia
Rimondi, Agustina
Gildea, Sarah
Miño, Samuel
Vissani, Maria Aldana
Carossino, Mariano
Cullinane, Ann
Barrandeguy, Maria Edith
author_role author
author2 Rimondi, Agustina
Gildea, Sarah
Miño, Samuel
Vissani, Maria Aldana
Carossino, Mariano
Cullinane, Ann
Barrandeguy, Maria Edith
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Equine Influenzavirus
Animal Diseases
Horses
Epidemiology
Virus de la Influenza Equina
Enfermedades de los Animales
Caballos
Epidemiología
América del Sur
topic Equine Influenzavirus
Animal Diseases
Horses
Epidemiology
Virus de la Influenza Equina
Enfermedades de los Animales
Caballos
Epidemiología
América del Sur
dc.description.none.fl_txt_mv Background: In 2012, equine influenza (EI) virus was confirmed as the cause of outbreaks of respiratory disease in horses throughout South America. In Uruguay and Argentina, hundreds of vaccinated thoroughbred horses in training and racing facilities were clinically affected. Objective: To characterise the EI viruses detected during the outbreak in Uruguay and Argentina. Methods: Virus was detected in nasopharyngeal swabs by a pan-reactive influenza type A real-time RT-PCR. The nucleotide sequence of the HA1 gene was determined and analysed phylogenetically using mega 5 software. Amino acid sequences alignments were constructed and virus was antigenically characterised with specific ferret antisera. Paired serum samples were tested by haemagglutination inhibition and single radial haemolysis. Results: The diagnosis of EIV was confirmed by real-time RT-PCR, virus isolation and serological testing. The phylogenetic analysis of HA1 gene sequences of 18 EI viruses indicated that all of them belong to clade 1 of the Florida sublineage of the American lineage and are closely related to viruses isolated in the United States in 2012. The HA1 of viruses identified in horses in racing facilities in Maroñas, Uruguay, and in Palermo, Argentina, displayed 100% amino acid sequence identity and were identical to that of a virus isolated in Dubai in 2012, from vaccinated endurance horses recently imported from Uruguay. Conclusions: The surveillance data reported illustrate the international spread of EI viruses and support the recommendations of the OIE expert surveillance panel to include viruses of the Florida sublineage in vaccines.
Fil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rimondi, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Gildea, Sarah. The Irish Equine Centre. Virology Unit; Irlanda
Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Carossino, Mariano. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos
Fil: Cullinane, Ann. The Irish Equine Centre. Virology Unit; Irlanda
Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina
description Background: In 2012, equine influenza (EI) virus was confirmed as the cause of outbreaks of respiratory disease in horses throughout South America. In Uruguay and Argentina, hundreds of vaccinated thoroughbred horses in training and racing facilities were clinically affected. Objective: To characterise the EI viruses detected during the outbreak in Uruguay and Argentina. Methods: Virus was detected in nasopharyngeal swabs by a pan-reactive influenza type A real-time RT-PCR. The nucleotide sequence of the HA1 gene was determined and analysed phylogenetically using mega 5 software. Amino acid sequences alignments were constructed and virus was antigenically characterised with specific ferret antisera. Paired serum samples were tested by haemagglutination inhibition and single radial haemolysis. Results: The diagnosis of EIV was confirmed by real-time RT-PCR, virus isolation and serological testing. The phylogenetic analysis of HA1 gene sequences of 18 EI viruses indicated that all of them belong to clade 1 of the Florida sublineage of the American lineage and are closely related to viruses isolated in the United States in 2012. The HA1 of viruses identified in horses in racing facilities in Maroñas, Uruguay, and in Palermo, Argentina, displayed 100% amino acid sequence identity and were identical to that of a virus isolated in Dubai in 2012, from vaccinated endurance horses recently imported from Uruguay. Conclusions: The surveillance data reported illustrate the international spread of EI viruses and support the recommendations of the OIE expert surveillance panel to include viruses of the Florida sublineage in vaccines.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
2017-09-14T14:53:57Z
2017-09-14T14:53:57Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1222
http://onlinelibrary.wiley.com/doi/10.1111/irv.12349/epdf
1750-2659
DOI: 10.1111/irv.12349
url http://hdl.handle.net/20.500.12123/1222
http://onlinelibrary.wiley.com/doi/10.1111/irv.12349/epdf
identifier_str_mv 1750-2659
DOI: 10.1111/irv.12349
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv 2012
dc.source.none.fl_str_mv Influenza and Other Respiratory Viruses 10 (1) : 37–46 (January 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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