Equine influenza virus in Asia: phylogeographic pattern and molecular features reveal circulation of an autochthonous lineage

Autores
Miño, Samuel; Mojsiejczuk, Laura; Guo, Wei; Zhang, Haili; Qi, Ting; Du, Cheng; Zhang, Xiang; Wang, Jingfei; Campos, Rodolfo; Wang, Xiaojun
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Equine influenza virus (EIV) causes severe acute respiratory disease in horses. Currently, the strains belonging to the H3N8 subtype are divided into two clades, Florida clade 1 (FC1) and Florida clade 2 (FC2), which emerged in 2002. Both FC1 and FC2 clades were reported in Asian and Middle East countries in the last decade. In this study, we described the evolution, epidemiology, and molecular characteristic of the EIV lineages, with focus on those detected in Asia from 2007 to 2017. The full genome phylogeny showed that FC1 and FC2 constituted separate and divergent lineages, without evidence of reassortment between the clades. While FC1 evolved as a single lineage, FC2 showed a divergent event around 2004 giving rise to two well-supported and coexisting sublineages, European and Asian. Furthermore, two different spread patterns of EIV in Asian countries were identified. The FC1 outbreaks were caused by independent introductions of EIV from the Americas, with the Asian isolates genetically similar to the contemporary American lineages. On the other hand, the FC2 strains detected in Asian mainland countries conformed to an autochthonous monophyletic group with a common ancestor dated in 2006 and showed evidence of an endemic circulation in a local host. Characteristic aminoacidic signature patterns were detected in all viral proteins in both Asian-FC1 and FC2 populations. Several changes were located at the top of the HA1 protein, inside or near antigenic sites. Further studies are needed to assess the potential impact of these antigenic changes in vaccination programs. IMPORTANCE The complex and continuous antigenic evolution of equine influenza viruses (EIVs) remains a major hurdle for vaccine development and the design of effective immunization programs. The present study provides a comprehensive analysis showing the EIV evolutionary dynamics, including the spread and circulation within the Asian continent and its relationship to global EIV populations over a 10-year period. Moreover, we provide a better understanding of EIV molecular evolution in Asian countries and its consequences on the antigenicity. The study underscores the association between the global horse movement and the circulation of EIV in this region. Understanding EIV evolution is imperative in order to mitigate the risk of outbreaks affecting the horse industry and to help with the selection of the viral strains to be included in the formulation of future vaccines.
Instituto de Virología
Fil: Miño, Samuel. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Mojsiejczuk, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología, Biotecnología y Genética. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guo, Wei. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China
Fil: Zhang, Haili. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China
Fil: Qi, Ting. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China
Fil: Du, Cheng. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China
Fil: Zhang, Xiang. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China
Fil: Wang, Jingfei. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China
Fil: Campos, Rodolfo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología, Biotecnología y Genética. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wang, Xiaojun. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China
Fuente
Journal of virology 93 (13) : e00116-19. (Julio 2019)
Materia
Equine Influenzavirus
Evolution
Vaccines
Phylogeny
Virus de la Influenza Equina
Evolución
Influenzavirus
Vacuna
Asia
Filogenia
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Equine influenza virus in Asia: phylogeographic pattern and molecular features reveal circulation of an autochthonous lineageMiño, SamuelMojsiejczuk, LauraGuo, WeiZhang, HailiQi, TingDu, ChengZhang, XiangWang, JingfeiCampos, RodolfoWang, XiaojunEquine InfluenzavirusEvolutionVaccinesPhylogenyVirus de la Influenza EquinaEvoluciónInfluenzavirusVacunaAsiaFilogeniaEquine influenza virus (EIV) causes severe acute respiratory disease in horses. Currently, the strains belonging to the H3N8 subtype are divided into two clades, Florida clade 1 (FC1) and Florida clade 2 (FC2), which emerged in 2002. Both FC1 and FC2 clades were reported in Asian and Middle East countries in the last decade. In this study, we described the evolution, epidemiology, and molecular characteristic of the EIV lineages, with focus on those detected in Asia from 2007 to 2017. The full genome phylogeny showed that FC1 and FC2 constituted separate and divergent lineages, without evidence of reassortment between the clades. While FC1 evolved as a single lineage, FC2 showed a divergent event around 2004 giving rise to two well-supported and coexisting sublineages, European and Asian. Furthermore, two different spread patterns of EIV in Asian countries were identified. The FC1 outbreaks were caused by independent introductions of EIV from the Americas, with the Asian isolates genetically similar to the contemporary American lineages. On the other hand, the FC2 strains detected in Asian mainland countries conformed to an autochthonous monophyletic group with a common ancestor dated in 2006 and showed evidence of an endemic circulation in a local host. Characteristic aminoacidic signature patterns were detected in all viral proteins in both Asian-FC1 and FC2 populations. Several changes were located at the top of the HA1 protein, inside or near antigenic sites. Further studies are needed to assess the potential impact of these antigenic changes in vaccination programs. IMPORTANCE The complex and continuous antigenic evolution of equine influenza viruses (EIVs) remains a major hurdle for vaccine development and the design of effective immunization programs. The present study provides a comprehensive analysis showing the EIV evolutionary dynamics, including the spread and circulation within the Asian continent and its relationship to global EIV populations over a 10-year period. Moreover, we provide a better understanding of EIV molecular evolution in Asian countries and its consequences on the antigenicity. The study underscores the association between the global horse movement and the circulation of EIV in this region. Understanding EIV evolution is imperative in order to mitigate the risk of outbreaks affecting the horse industry and to help with the selection of the viral strains to be included in the formulation of future vaccines.Instituto de VirologíaFil: Miño, Samuel. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Mojsiejczuk, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología, Biotecnología y Genética. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Guo, Wei. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; ChinaFil: Zhang, Haili. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; ChinaFil: Qi, Ting. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; ChinaFil: Du, Cheng. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; ChinaFil: Zhang, Xiang. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; ChinaFil: Wang, Jingfei. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; ChinaFil: Campos, Rodolfo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología, Biotecnología y Genética. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wang, Xiaojun. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; ChinaAmerican Society for Microbiology2020-02-10T15:59:23Z2020-02-10T15:59:23Z2019-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/6711https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580976/0022-538Xhttps://doi.org/10 .1128/JVI.00116-19.Journal of virology 93 (13) : e00116-19. (Julio 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:52Zoai:localhost:20.500.12123/6711instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:52.468INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Equine influenza virus in Asia: phylogeographic pattern and molecular features reveal circulation of an autochthonous lineage
title Equine influenza virus in Asia: phylogeographic pattern and molecular features reveal circulation of an autochthonous lineage
spellingShingle Equine influenza virus in Asia: phylogeographic pattern and molecular features reveal circulation of an autochthonous lineage
Miño, Samuel
Equine Influenzavirus
Evolution
Vaccines
Phylogeny
Virus de la Influenza Equina
Evolución
Influenzavirus
Vacuna
Asia
Filogenia
title_short Equine influenza virus in Asia: phylogeographic pattern and molecular features reveal circulation of an autochthonous lineage
title_full Equine influenza virus in Asia: phylogeographic pattern and molecular features reveal circulation of an autochthonous lineage
title_fullStr Equine influenza virus in Asia: phylogeographic pattern and molecular features reveal circulation of an autochthonous lineage
title_full_unstemmed Equine influenza virus in Asia: phylogeographic pattern and molecular features reveal circulation of an autochthonous lineage
title_sort Equine influenza virus in Asia: phylogeographic pattern and molecular features reveal circulation of an autochthonous lineage
dc.creator.none.fl_str_mv Miño, Samuel
Mojsiejczuk, Laura
Guo, Wei
Zhang, Haili
Qi, Ting
Du, Cheng
Zhang, Xiang
Wang, Jingfei
Campos, Rodolfo
Wang, Xiaojun
author Miño, Samuel
author_facet Miño, Samuel
Mojsiejczuk, Laura
Guo, Wei
Zhang, Haili
Qi, Ting
Du, Cheng
Zhang, Xiang
Wang, Jingfei
Campos, Rodolfo
Wang, Xiaojun
author_role author
author2 Mojsiejczuk, Laura
Guo, Wei
Zhang, Haili
Qi, Ting
Du, Cheng
Zhang, Xiang
Wang, Jingfei
Campos, Rodolfo
Wang, Xiaojun
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Equine Influenzavirus
Evolution
Vaccines
Phylogeny
Virus de la Influenza Equina
Evolución
Influenzavirus
Vacuna
Asia
Filogenia
topic Equine Influenzavirus
Evolution
Vaccines
Phylogeny
Virus de la Influenza Equina
Evolución
Influenzavirus
Vacuna
Asia
Filogenia
dc.description.none.fl_txt_mv Equine influenza virus (EIV) causes severe acute respiratory disease in horses. Currently, the strains belonging to the H3N8 subtype are divided into two clades, Florida clade 1 (FC1) and Florida clade 2 (FC2), which emerged in 2002. Both FC1 and FC2 clades were reported in Asian and Middle East countries in the last decade. In this study, we described the evolution, epidemiology, and molecular characteristic of the EIV lineages, with focus on those detected in Asia from 2007 to 2017. The full genome phylogeny showed that FC1 and FC2 constituted separate and divergent lineages, without evidence of reassortment between the clades. While FC1 evolved as a single lineage, FC2 showed a divergent event around 2004 giving rise to two well-supported and coexisting sublineages, European and Asian. Furthermore, two different spread patterns of EIV in Asian countries were identified. The FC1 outbreaks were caused by independent introductions of EIV from the Americas, with the Asian isolates genetically similar to the contemporary American lineages. On the other hand, the FC2 strains detected in Asian mainland countries conformed to an autochthonous monophyletic group with a common ancestor dated in 2006 and showed evidence of an endemic circulation in a local host. Characteristic aminoacidic signature patterns were detected in all viral proteins in both Asian-FC1 and FC2 populations. Several changes were located at the top of the HA1 protein, inside or near antigenic sites. Further studies are needed to assess the potential impact of these antigenic changes in vaccination programs. IMPORTANCE The complex and continuous antigenic evolution of equine influenza viruses (EIVs) remains a major hurdle for vaccine development and the design of effective immunization programs. The present study provides a comprehensive analysis showing the EIV evolutionary dynamics, including the spread and circulation within the Asian continent and its relationship to global EIV populations over a 10-year period. Moreover, we provide a better understanding of EIV molecular evolution in Asian countries and its consequences on the antigenicity. The study underscores the association between the global horse movement and the circulation of EIV in this region. Understanding EIV evolution is imperative in order to mitigate the risk of outbreaks affecting the horse industry and to help with the selection of the viral strains to be included in the formulation of future vaccines.
Instituto de Virología
Fil: Miño, Samuel. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Mojsiejczuk, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología, Biotecnología y Genética. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guo, Wei. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China
Fil: Zhang, Haili. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China
Fil: Qi, Ting. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China
Fil: Du, Cheng. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China
Fil: Zhang, Xiang. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China
Fil: Wang, Jingfei. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China
Fil: Campos, Rodolfo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología, Biotecnología y Genética. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wang, Xiaojun. Chinese Academy of Agricultural Sciences. Harbin Veterinary Research Institute. National Key Laboratory of Veterinary Biotechnology; China
description Equine influenza virus (EIV) causes severe acute respiratory disease in horses. Currently, the strains belonging to the H3N8 subtype are divided into two clades, Florida clade 1 (FC1) and Florida clade 2 (FC2), which emerged in 2002. Both FC1 and FC2 clades were reported in Asian and Middle East countries in the last decade. In this study, we described the evolution, epidemiology, and molecular characteristic of the EIV lineages, with focus on those detected in Asia from 2007 to 2017. The full genome phylogeny showed that FC1 and FC2 constituted separate and divergent lineages, without evidence of reassortment between the clades. While FC1 evolved as a single lineage, FC2 showed a divergent event around 2004 giving rise to two well-supported and coexisting sublineages, European and Asian. Furthermore, two different spread patterns of EIV in Asian countries were identified. The FC1 outbreaks were caused by independent introductions of EIV from the Americas, with the Asian isolates genetically similar to the contemporary American lineages. On the other hand, the FC2 strains detected in Asian mainland countries conformed to an autochthonous monophyletic group with a common ancestor dated in 2006 and showed evidence of an endemic circulation in a local host. Characteristic aminoacidic signature patterns were detected in all viral proteins in both Asian-FC1 and FC2 populations. Several changes were located at the top of the HA1 protein, inside or near antigenic sites. Further studies are needed to assess the potential impact of these antigenic changes in vaccination programs. IMPORTANCE The complex and continuous antigenic evolution of equine influenza viruses (EIVs) remains a major hurdle for vaccine development and the design of effective immunization programs. The present study provides a comprehensive analysis showing the EIV evolutionary dynamics, including the spread and circulation within the Asian continent and its relationship to global EIV populations over a 10-year period. Moreover, we provide a better understanding of EIV molecular evolution in Asian countries and its consequences on the antigenicity. The study underscores the association between the global horse movement and the circulation of EIV in this region. Understanding EIV evolution is imperative in order to mitigate the risk of outbreaks affecting the horse industry and to help with the selection of the viral strains to be included in the formulation of future vaccines.
publishDate 2019
dc.date.none.fl_str_mv 2019-07
2020-02-10T15:59:23Z
2020-02-10T15:59:23Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/6711
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580976/
0022-538X
https://doi.org/10 .1128/JVI.00116-19.
url http://hdl.handle.net/20.500.12123/6711
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580976/
https://doi.org/10 .1128/JVI.00116-19.
identifier_str_mv 0022-538X
dc.language.none.fl_str_mv eng
language eng
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv Journal of virology 93 (13) : e00116-19. (Julio 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
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