First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina

Autores
Olguin Perglione, Cecilia; Politzki, Romina Paula; Alvarez, Irene; Ruiz, Vanesa
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The novel Equine Parvovirus-Hepatitis (EqPV-H) was first identified in the serum and liver of a horse that died of equine serum hepatitis, also known as Theiler's disease. Several reports in recent years strongly suggest that EqPV-H is the etiologic agent of Theiler's disease. Brazil is the only South American country where infection with this virus has been reported. This study investigated the presence of EqPV-H DNA in horse serum pools (n=51), commercial horse serum batches (n=5) and individual serum samples from donor horses (n=175) from Argentina. All serum samples were analyzed by quantitative polymerase chain reaction (qPCR) and samples with positive or indeterminate results were further analyzed by NS1 nested-PCR for phylogenetic studies. None of the serum pools was positive by qPCR but 9/51 pools were indeterminate (one or both test sample’s Ct values were higher than the limit of detection). The NS1 nested-PCR detected the EqPV-H DNA in 8 of these indeterminate samples (15.7 % of serum pools). Three of the commercial horse serum batches (60 %) contained EqPV-H DNA, detected either by qPCR and/or nested-PCR. From the 175 individual horse serum samples, three (1.71 %) were positive for EqPV-H by both techniques. The genetic analysis of the 12 partial NS1 sequences obtained showed that the local isolates were similar to EqPV-H sequences from Germany and China. This study provides the first evidence of the presence of EqPV-H in horses and in horse sera commercially available in Argentina and emphasizes the importance of controlling the biosecurity of commercial equine sera as well as any other blood-derived biological products of equine origin.
Instituto de Virología
Fil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina
Fil: Olguin Perglione, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina
Fil: Politzki, Romina Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina
Fil: Politzki, Romina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina
Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina
Fil: Alvarez, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina
Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina
Fil: Ruiz, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina
Fuente
Veterinary journal 306 : 106204. (22 July 2024)
Materia
Horses
Animal Diseases
Phylogeny
Caballo
Enfermedades de los Animales
Parvoviridae
Hepatitis
Filogenia
Argentina
Equine Parvovirus-Hepatitis
Serum Pools
Parvovirus Equino Hepatitis
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/18692
Acceder
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oai_identifier_str oai:localhost:20.500.12123/18692
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling First report of Equine Parvovirus-Hepatitis (EqPV-H) in ArgentinaOlguin Perglione, CeciliaPolitzki, Romina PaulaAlvarez, IreneRuiz, VanesaHorsesAnimal DiseasesPhylogenyCaballoEnfermedades de los AnimalesParvoviridaeHepatitisFilogeniaArgentinaEquine Parvovirus-HepatitisSerum PoolsParvovirus Equino HepatitisThe novel Equine Parvovirus-Hepatitis (EqPV-H) was first identified in the serum and liver of a horse that died of equine serum hepatitis, also known as Theiler's disease. Several reports in recent years strongly suggest that EqPV-H is the etiologic agent of Theiler's disease. Brazil is the only South American country where infection with this virus has been reported. This study investigated the presence of EqPV-H DNA in horse serum pools (n=51), commercial horse serum batches (n=5) and individual serum samples from donor horses (n=175) from Argentina. All serum samples were analyzed by quantitative polymerase chain reaction (qPCR) and samples with positive or indeterminate results were further analyzed by NS1 nested-PCR for phylogenetic studies. None of the serum pools was positive by qPCR but 9/51 pools were indeterminate (one or both test sample’s Ct values were higher than the limit of detection). The NS1 nested-PCR detected the EqPV-H DNA in 8 of these indeterminate samples (15.7 % of serum pools). Three of the commercial horse serum batches (60 %) contained EqPV-H DNA, detected either by qPCR and/or nested-PCR. From the 175 individual horse serum samples, three (1.71 %) were positive for EqPV-H by both techniques. The genetic analysis of the 12 partial NS1 sequences obtained showed that the local isolates were similar to EqPV-H sequences from Germany and China. This study provides the first evidence of the presence of EqPV-H in horses and in horse sera commercially available in Argentina and emphasizes the importance of controlling the biosecurity of commercial equine sera as well as any other blood-derived biological products of equine origin.Instituto de VirologíaFil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); ArgentinaFil: Olguin Perglione, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); ArgentinaFil: Politzki, Romina Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); ArgentinaFil: Politzki, Romina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); ArgentinaFil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); ArgentinaFil: Alvarez, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); ArgentinaFil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); ArgentinaFil: Ruiz, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); ArgentinaElsevier2024-07-29T10:38:21Z2024-07-29T10:38:21Z2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/18692https://www.sciencedirect.com/science/article/abs/pii/S10900233240014361090-0233https://doi.org/10.1016/j.tvjl.2024.106204Veterinary journal 306 : 106204. (22 July 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/reference/hdl/20.500.12123/18696info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una SaludArgentina .......... (nation) (World, South America)7006477info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-11T10:25:14Zoai:localhost:20.500.12123/18692instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:25:14.491INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina
title First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina
spellingShingle First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina
Olguin Perglione, Cecilia
Horses
Animal Diseases
Phylogeny
Caballo
Enfermedades de los Animales
Parvoviridae
Hepatitis
Filogenia
Argentina
Equine Parvovirus-Hepatitis
Serum Pools
Parvovirus Equino Hepatitis
title_short First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina
title_full First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina
title_fullStr First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina
title_full_unstemmed First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina
title_sort First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina
dc.creator.none.fl_str_mv Olguin Perglione, Cecilia
Politzki, Romina Paula
Alvarez, Irene
Ruiz, Vanesa
author Olguin Perglione, Cecilia
author_facet Olguin Perglione, Cecilia
Politzki, Romina Paula
Alvarez, Irene
Ruiz, Vanesa
author_role author
author2 Politzki, Romina Paula
Alvarez, Irene
Ruiz, Vanesa
author2_role author
author
author
dc.subject.none.fl_str_mv Horses
Animal Diseases
Phylogeny
Caballo
Enfermedades de los Animales
Parvoviridae
Hepatitis
Filogenia
Argentina
Equine Parvovirus-Hepatitis
Serum Pools
Parvovirus Equino Hepatitis
topic Horses
Animal Diseases
Phylogeny
Caballo
Enfermedades de los Animales
Parvoviridae
Hepatitis
Filogenia
Argentina
Equine Parvovirus-Hepatitis
Serum Pools
Parvovirus Equino Hepatitis
dc.description.none.fl_txt_mv The novel Equine Parvovirus-Hepatitis (EqPV-H) was first identified in the serum and liver of a horse that died of equine serum hepatitis, also known as Theiler's disease. Several reports in recent years strongly suggest that EqPV-H is the etiologic agent of Theiler's disease. Brazil is the only South American country where infection with this virus has been reported. This study investigated the presence of EqPV-H DNA in horse serum pools (n=51), commercial horse serum batches (n=5) and individual serum samples from donor horses (n=175) from Argentina. All serum samples were analyzed by quantitative polymerase chain reaction (qPCR) and samples with positive or indeterminate results were further analyzed by NS1 nested-PCR for phylogenetic studies. None of the serum pools was positive by qPCR but 9/51 pools were indeterminate (one or both test sample’s Ct values were higher than the limit of detection). The NS1 nested-PCR detected the EqPV-H DNA in 8 of these indeterminate samples (15.7 % of serum pools). Three of the commercial horse serum batches (60 %) contained EqPV-H DNA, detected either by qPCR and/or nested-PCR. From the 175 individual horse serum samples, three (1.71 %) were positive for EqPV-H by both techniques. The genetic analysis of the 12 partial NS1 sequences obtained showed that the local isolates were similar to EqPV-H sequences from Germany and China. This study provides the first evidence of the presence of EqPV-H in horses and in horse sera commercially available in Argentina and emphasizes the importance of controlling the biosecurity of commercial equine sera as well as any other blood-derived biological products of equine origin.
Instituto de Virología
Fil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina
Fil: Olguin Perglione, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina
Fil: Politzki, Romina Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina
Fil: Politzki, Romina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina
Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina
Fil: Alvarez, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina
Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina
Fil: Ruiz, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina
description The novel Equine Parvovirus-Hepatitis (EqPV-H) was first identified in the serum and liver of a horse that died of equine serum hepatitis, also known as Theiler's disease. Several reports in recent years strongly suggest that EqPV-H is the etiologic agent of Theiler's disease. Brazil is the only South American country where infection with this virus has been reported. This study investigated the presence of EqPV-H DNA in horse serum pools (n=51), commercial horse serum batches (n=5) and individual serum samples from donor horses (n=175) from Argentina. All serum samples were analyzed by quantitative polymerase chain reaction (qPCR) and samples with positive or indeterminate results were further analyzed by NS1 nested-PCR for phylogenetic studies. None of the serum pools was positive by qPCR but 9/51 pools were indeterminate (one or both test sample’s Ct values were higher than the limit of detection). The NS1 nested-PCR detected the EqPV-H DNA in 8 of these indeterminate samples (15.7 % of serum pools). Three of the commercial horse serum batches (60 %) contained EqPV-H DNA, detected either by qPCR and/or nested-PCR. From the 175 individual horse serum samples, three (1.71 %) were positive for EqPV-H by both techniques. The genetic analysis of the 12 partial NS1 sequences obtained showed that the local isolates were similar to EqPV-H sequences from Germany and China. This study provides the first evidence of the presence of EqPV-H in horses and in horse sera commercially available in Argentina and emphasizes the importance of controlling the biosecurity of commercial equine sera as well as any other blood-derived biological products of equine origin.
publishDate 2024
dc.date.none.fl_str_mv 2024-07-29T10:38:21Z
2024-07-29T10:38:21Z
2024-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/18692
https://www.sciencedirect.com/science/article/abs/pii/S1090023324001436
1090-0233
https://doi.org/10.1016/j.tvjl.2024.106204
url http://hdl.handle.net/20.500.12123/18692
https://www.sciencedirect.com/science/article/abs/pii/S1090023324001436
https://doi.org/10.1016/j.tvjl.2024.106204
identifier_str_mv 1090-0233
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/reference/hdl/20.500.12123/18696
info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina .......... (nation) (World, South America)
7006477
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary journal 306 : 106204. (22 July 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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