Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae)

Autores
Rolandi, Carmen; Roca-Acevedo, Gonzalo; Schilman, Pablo Ernesto; Germano, Monica Daniela
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Triatoma infestans (Klug, 1834), the main vector of Chagas disease in Latin America, is regularly controlled by spraying the pyrethroid deltamethrin, to which some populations have developed resistance. The three main mechanisms of resistance are 1) metabolic resistance by overexpression or increased activity of detoxifying enzymes, 2) target site mutations, and 3) cuticle thickening/modification. We use open-flow respirometry to measure real-time H2O loss rate (V˙ H2O) and CO2 production rate (V˙ CO2), on nymphs from susceptible and resistant populations before and after exposure to the insecticide to understand the underlying mechanisms of resistance in live insects. Lack of differences in V˙ H2O between populations suggested that cuticular thickness/composition is not acting as a relevant resistance mechanism. Similarly, there was no difference in resting V˙ CO2, suggesting a trade-off between resistance mechanisms and other physiological processes. The increment in V˙ CO2 after application of deltamethrin was similar in both populations, which suggested that while enhanced enzymatic detoxification may play a role in resistance expression in this population, the main mechanism involved should be a passive one such as target site mutations. Open-flow respirometry provided useful evidence for evaluating the mechanisms involved in deltamethrin resistance. Using this technique could improve efficiency of scientific research in the area of insecticide resistance management, leading to a faster decision making and hence improved control results.
Estación Experimental Agropecuaria Bariloche
Fil: Rolandi, Carmen. Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Ecofisiología de Insectos; Argentina
Fil: Roca-Acevedo, Gonzalo. Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones de Plagas e Insecticidas; Argentina
Fil: Schilman, Pablo Ernesto. Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Ecofisiología de Insectos; Argentina
Fil: Germano, Monica Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Campo Forestal General San Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fuente
Journal of Medical Entomology : art: tjaa099 (Junio 2020)
Materia
Triatoma Infestans
Enfermedad de Chagas
Insecta
Resistencia a los Insecticidas
Chagas' disease
Insecticide Resistance
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7860
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oai_identifier_str oai:localhost:20.500.12123/7860
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spelling Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae)Rolandi, CarmenRoca-Acevedo, GonzaloSchilman, Pablo ErnestoGermano, Monica DanielaTriatoma InfestansEnfermedad de ChagasInsectaResistencia a los InsecticidasChagas' diseaseInsecticide ResistanceTriatoma infestans (Klug, 1834), the main vector of Chagas disease in Latin America, is regularly controlled by spraying the pyrethroid deltamethrin, to which some populations have developed resistance. The three main mechanisms of resistance are 1) metabolic resistance by overexpression or increased activity of detoxifying enzymes, 2) target site mutations, and 3) cuticle thickening/modification. We use open-flow respirometry to measure real-time H2O loss rate (V˙ H2O) and CO2 production rate (V˙ CO2), on nymphs from susceptible and resistant populations before and after exposure to the insecticide to understand the underlying mechanisms of resistance in live insects. Lack of differences in V˙ H2O between populations suggested that cuticular thickness/composition is not acting as a relevant resistance mechanism. Similarly, there was no difference in resting V˙ CO2, suggesting a trade-off between resistance mechanisms and other physiological processes. The increment in V˙ CO2 after application of deltamethrin was similar in both populations, which suggested that while enhanced enzymatic detoxification may play a role in resistance expression in this population, the main mechanism involved should be a passive one such as target site mutations. Open-flow respirometry provided useful evidence for evaluating the mechanisms involved in deltamethrin resistance. Using this technique could improve efficiency of scientific research in the area of insecticide resistance management, leading to a faster decision making and hence improved control results.Estación Experimental Agropecuaria BarilocheFil: Rolandi, Carmen. Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Ecofisiología de Insectos; ArgentinaFil: Roca-Acevedo, Gonzalo. Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones de Plagas e Insecticidas; ArgentinaFil: Schilman, Pablo Ernesto. Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Ecofisiología de Insectos; ArgentinaFil: Germano, Monica Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Campo Forestal General San Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaEntomological Society of America2020-09-10T11:27:50Z2020-09-10T11:27:50Z2020-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7860https://academic.oup.com/jme/advance-article-abstract/doi/10.1093/jme/tjaa099/58589311938-29280022-2585https://doi.org/10.1093/jme/tjaa099Journal of Medical Entomology : art: tjaa099 (Junio 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:52Zoai:localhost:20.500.12123/7860instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:52.483INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae)
title Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae)
spellingShingle Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae)
Rolandi, Carmen
Triatoma Infestans
Enfermedad de Chagas
Insecta
Resistencia a los Insecticidas
Chagas' disease
Insecticide Resistance
title_short Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae)
title_full Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae)
title_fullStr Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae)
title_full_unstemmed Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae)
title_sort Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae)
dc.creator.none.fl_str_mv Rolandi, Carmen
Roca-Acevedo, Gonzalo
Schilman, Pablo Ernesto
Germano, Monica Daniela
author Rolandi, Carmen
author_facet Rolandi, Carmen
Roca-Acevedo, Gonzalo
Schilman, Pablo Ernesto
Germano, Monica Daniela
author_role author
author2 Roca-Acevedo, Gonzalo
Schilman, Pablo Ernesto
Germano, Monica Daniela
author2_role author
author
author
dc.subject.none.fl_str_mv Triatoma Infestans
Enfermedad de Chagas
Insecta
Resistencia a los Insecticidas
Chagas' disease
Insecticide Resistance
topic Triatoma Infestans
Enfermedad de Chagas
Insecta
Resistencia a los Insecticidas
Chagas' disease
Insecticide Resistance
dc.description.none.fl_txt_mv Triatoma infestans (Klug, 1834), the main vector of Chagas disease in Latin America, is regularly controlled by spraying the pyrethroid deltamethrin, to which some populations have developed resistance. The three main mechanisms of resistance are 1) metabolic resistance by overexpression or increased activity of detoxifying enzymes, 2) target site mutations, and 3) cuticle thickening/modification. We use open-flow respirometry to measure real-time H2O loss rate (V˙ H2O) and CO2 production rate (V˙ CO2), on nymphs from susceptible and resistant populations before and after exposure to the insecticide to understand the underlying mechanisms of resistance in live insects. Lack of differences in V˙ H2O between populations suggested that cuticular thickness/composition is not acting as a relevant resistance mechanism. Similarly, there was no difference in resting V˙ CO2, suggesting a trade-off between resistance mechanisms and other physiological processes. The increment in V˙ CO2 after application of deltamethrin was similar in both populations, which suggested that while enhanced enzymatic detoxification may play a role in resistance expression in this population, the main mechanism involved should be a passive one such as target site mutations. Open-flow respirometry provided useful evidence for evaluating the mechanisms involved in deltamethrin resistance. Using this technique could improve efficiency of scientific research in the area of insecticide resistance management, leading to a faster decision making and hence improved control results.
Estación Experimental Agropecuaria Bariloche
Fil: Rolandi, Carmen. Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Ecofisiología de Insectos; Argentina
Fil: Roca-Acevedo, Gonzalo. Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones de Plagas e Insecticidas; Argentina
Fil: Schilman, Pablo Ernesto. Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Ecofisiología de Insectos; Argentina
Fil: Germano, Monica Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Campo Forestal General San Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
description Triatoma infestans (Klug, 1834), the main vector of Chagas disease in Latin America, is regularly controlled by spraying the pyrethroid deltamethrin, to which some populations have developed resistance. The three main mechanisms of resistance are 1) metabolic resistance by overexpression or increased activity of detoxifying enzymes, 2) target site mutations, and 3) cuticle thickening/modification. We use open-flow respirometry to measure real-time H2O loss rate (V˙ H2O) and CO2 production rate (V˙ CO2), on nymphs from susceptible and resistant populations before and after exposure to the insecticide to understand the underlying mechanisms of resistance in live insects. Lack of differences in V˙ H2O between populations suggested that cuticular thickness/composition is not acting as a relevant resistance mechanism. Similarly, there was no difference in resting V˙ CO2, suggesting a trade-off between resistance mechanisms and other physiological processes. The increment in V˙ CO2 after application of deltamethrin was similar in both populations, which suggested that while enhanced enzymatic detoxification may play a role in resistance expression in this population, the main mechanism involved should be a passive one such as target site mutations. Open-flow respirometry provided useful evidence for evaluating the mechanisms involved in deltamethrin resistance. Using this technique could improve efficiency of scientific research in the area of insecticide resistance management, leading to a faster decision making and hence improved control results.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-10T11:27:50Z
2020-09-10T11:27:50Z
2020-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7860
https://academic.oup.com/jme/advance-article-abstract/doi/10.1093/jme/tjaa099/5858931
1938-2928
0022-2585
https://doi.org/10.1093/jme/tjaa099
url http://hdl.handle.net/20.500.12123/7860
https://academic.oup.com/jme/advance-article-abstract/doi/10.1093/jme/tjaa099/5858931
https://doi.org/10.1093/jme/tjaa099
identifier_str_mv 1938-2928
0022-2585
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Entomological Society of America
publisher.none.fl_str_mv Entomological Society of America
dc.source.none.fl_str_mv Journal of Medical Entomology : art: tjaa099 (Junio 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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