Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroids

Autores
Traverso, Lucila María; Latorre Estivalis, Jose Manuel; Rocha Fernandes, Gabriel da; Fronza, Georgina; Lobbia, Patricia Alejandra; Mougabure Cueto, Gastón Adolfo; Ons, Sheila
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background Triatoma infestans is the main vector of Chagas disease in the Southern Cone. The resistance to pyrethroid insecticides developed by populations of this species impairs the effectiveness of vector control campaigns in wide regions of Argentina. The study of the global transcriptomic response to pyrethroid insecticides is important to deepen the knowledge about detoxification in triatomines. Methodology and findings We used RNA-Seq to explore the early transcriptomic response after intoxication with deltamethrin in a population of T. infestans which presents low resistance to pyrethroids. We were able to assemble a complete transcriptome of this vector and found evidence of differentially expressed genes belonging to diverse families such as chemosensory and odorantbinding proteins, ABC transporters and heat-shock proteins. Moreover, genes related to transcription and translation, energetic metabolism and cuticle rearrangements were also modulated. Finally, we characterized the repertoire of previously uncharacterized detoxification-related gene families in T. infestans and Rhodnius prolixus. Conclusions and significance Our work contributes to the understanding of the detoxification response in vectors of Chagas disease. Given the absence of an annotated genome from T. infestans, the analysis presented here constitutes a resource for molecular and physiological studies in this species. The results increase the knowledge on detoxification processes in vectors of Chagas disease, and provide relevant information to explore undescribed potential insecticide resistance mechanisms in populations of these insects.
Fil: Traverso, Lucila María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. (conicet - Unlp). Grupo Vinculado Laboratorio de Neurobiologia de Insectos (g.v. Al Cenexa); Argentina
Fil: Latorre Estivalis, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio del Grupo de Estudio de Insectos Sociales; Argentina
Fil: Rocha Fernandes, Gabriel da. Instituto René Rachou; Brasil
Fil: Fronza, Georgina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Instituto de Investigaciones e Ingeniería Ambiental. Laboratorio de Ecología de Enfermedades Transmitidas por Vectores; Argentina
Fil: Lobbia, Patricia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ministerio de Salud. Dirección de Enfermedades Transmisibles por Vectores. Centro de Referencia de Vectores; Argentina
Fil: Mougabure Cueto, Gastón Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ministerio de Salud. Dirección de Enfermedades Transmisibles por Vectores. Centro de Referencia de Vectores; Argentina
Fil: Ons, Sheila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. (conicet - Unlp). Grupo Vinculado Laboratorio de Neurobiologia de Insectos (g.v. Al Cenexa); Argentina
Materia
TRIATOMA INFESTANS
INSECTICIDE
TRANSCRIPTOMICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189397
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroidsTraverso, Lucila MaríaLatorre Estivalis, Jose ManuelRocha Fernandes, Gabriel daFronza, GeorginaLobbia, Patricia AlejandraMougabure Cueto, Gastón AdolfoOns, SheilaTRIATOMA INFESTANSINSECTICIDETRANSCRIPTOMICShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background Triatoma infestans is the main vector of Chagas disease in the Southern Cone. The resistance to pyrethroid insecticides developed by populations of this species impairs the effectiveness of vector control campaigns in wide regions of Argentina. The study of the global transcriptomic response to pyrethroid insecticides is important to deepen the knowledge about detoxification in triatomines. Methodology and findings We used RNA-Seq to explore the early transcriptomic response after intoxication with deltamethrin in a population of T. infestans which presents low resistance to pyrethroids. We were able to assemble a complete transcriptome of this vector and found evidence of differentially expressed genes belonging to diverse families such as chemosensory and odorantbinding proteins, ABC transporters and heat-shock proteins. Moreover, genes related to transcription and translation, energetic metabolism and cuticle rearrangements were also modulated. Finally, we characterized the repertoire of previously uncharacterized detoxification-related gene families in T. infestans and Rhodnius prolixus. Conclusions and significance Our work contributes to the understanding of the detoxification response in vectors of Chagas disease. Given the absence of an annotated genome from T. infestans, the analysis presented here constitutes a resource for molecular and physiological studies in this species. The results increase the knowledge on detoxification processes in vectors of Chagas disease, and provide relevant information to explore undescribed potential insecticide resistance mechanisms in populations of these insects.Fil: Traverso, Lucila María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. (conicet - Unlp). Grupo Vinculado Laboratorio de Neurobiologia de Insectos (g.v. Al Cenexa); ArgentinaFil: Latorre Estivalis, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio del Grupo de Estudio de Insectos Sociales; ArgentinaFil: Rocha Fernandes, Gabriel da. Instituto René Rachou; BrasilFil: Fronza, Georgina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Instituto de Investigaciones e Ingeniería Ambiental. Laboratorio de Ecología de Enfermedades Transmitidas por Vectores; ArgentinaFil: Lobbia, Patricia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ministerio de Salud. Dirección de Enfermedades Transmisibles por Vectores. Centro de Referencia de Vectores; ArgentinaFil: Mougabure Cueto, Gastón Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ministerio de Salud. Dirección de Enfermedades Transmisibles por Vectores. Centro de Referencia de Vectores; ArgentinaFil: Ons, Sheila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. (conicet - Unlp). Grupo Vinculado Laboratorio de Neurobiologia de Insectos (g.v. Al Cenexa); ArgentinaPublic Library of Science2022-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189397Traverso, Lucila María; Latorre Estivalis, Jose Manuel; Rocha Fernandes, Gabriel da; Fronza, Georgina; Lobbia, Patricia Alejandra; et al.; Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroids; Public Library of Science; Neglected Tropical Diseases; 16; 6; 6-2022; 1-271935-2735CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0010060info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0010060info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:35Zoai:ri.conicet.gov.ar:11336/189397instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:36.051CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroids
title Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroids
spellingShingle Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroids
Traverso, Lucila María
TRIATOMA INFESTANS
INSECTICIDE
TRANSCRIPTOMICS
title_short Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroids
title_full Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroids
title_fullStr Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroids
title_full_unstemmed Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroids
title_sort Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroids
dc.creator.none.fl_str_mv Traverso, Lucila María
Latorre Estivalis, Jose Manuel
Rocha Fernandes, Gabriel da
Fronza, Georgina
Lobbia, Patricia Alejandra
Mougabure Cueto, Gastón Adolfo
Ons, Sheila
author Traverso, Lucila María
author_facet Traverso, Lucila María
Latorre Estivalis, Jose Manuel
Rocha Fernandes, Gabriel da
Fronza, Georgina
Lobbia, Patricia Alejandra
Mougabure Cueto, Gastón Adolfo
Ons, Sheila
author_role author
author2 Latorre Estivalis, Jose Manuel
Rocha Fernandes, Gabriel da
Fronza, Georgina
Lobbia, Patricia Alejandra
Mougabure Cueto, Gastón Adolfo
Ons, Sheila
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv TRIATOMA INFESTANS
INSECTICIDE
TRANSCRIPTOMICS
topic TRIATOMA INFESTANS
INSECTICIDE
TRANSCRIPTOMICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background Triatoma infestans is the main vector of Chagas disease in the Southern Cone. The resistance to pyrethroid insecticides developed by populations of this species impairs the effectiveness of vector control campaigns in wide regions of Argentina. The study of the global transcriptomic response to pyrethroid insecticides is important to deepen the knowledge about detoxification in triatomines. Methodology and findings We used RNA-Seq to explore the early transcriptomic response after intoxication with deltamethrin in a population of T. infestans which presents low resistance to pyrethroids. We were able to assemble a complete transcriptome of this vector and found evidence of differentially expressed genes belonging to diverse families such as chemosensory and odorantbinding proteins, ABC transporters and heat-shock proteins. Moreover, genes related to transcription and translation, energetic metabolism and cuticle rearrangements were also modulated. Finally, we characterized the repertoire of previously uncharacterized detoxification-related gene families in T. infestans and Rhodnius prolixus. Conclusions and significance Our work contributes to the understanding of the detoxification response in vectors of Chagas disease. Given the absence of an annotated genome from T. infestans, the analysis presented here constitutes a resource for molecular and physiological studies in this species. The results increase the knowledge on detoxification processes in vectors of Chagas disease, and provide relevant information to explore undescribed potential insecticide resistance mechanisms in populations of these insects.
Fil: Traverso, Lucila María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. (conicet - Unlp). Grupo Vinculado Laboratorio de Neurobiologia de Insectos (g.v. Al Cenexa); Argentina
Fil: Latorre Estivalis, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio del Grupo de Estudio de Insectos Sociales; Argentina
Fil: Rocha Fernandes, Gabriel da. Instituto René Rachou; Brasil
Fil: Fronza, Georgina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Instituto de Investigaciones e Ingeniería Ambiental. Laboratorio de Ecología de Enfermedades Transmitidas por Vectores; Argentina
Fil: Lobbia, Patricia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ministerio de Salud. Dirección de Enfermedades Transmisibles por Vectores. Centro de Referencia de Vectores; Argentina
Fil: Mougabure Cueto, Gastón Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ministerio de Salud. Dirección de Enfermedades Transmisibles por Vectores. Centro de Referencia de Vectores; Argentina
Fil: Ons, Sheila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. (conicet - Unlp). Grupo Vinculado Laboratorio de Neurobiologia de Insectos (g.v. Al Cenexa); Argentina
description Background Triatoma infestans is the main vector of Chagas disease in the Southern Cone. The resistance to pyrethroid insecticides developed by populations of this species impairs the effectiveness of vector control campaigns in wide regions of Argentina. The study of the global transcriptomic response to pyrethroid insecticides is important to deepen the knowledge about detoxification in triatomines. Methodology and findings We used RNA-Seq to explore the early transcriptomic response after intoxication with deltamethrin in a population of T. infestans which presents low resistance to pyrethroids. We were able to assemble a complete transcriptome of this vector and found evidence of differentially expressed genes belonging to diverse families such as chemosensory and odorantbinding proteins, ABC transporters and heat-shock proteins. Moreover, genes related to transcription and translation, energetic metabolism and cuticle rearrangements were also modulated. Finally, we characterized the repertoire of previously uncharacterized detoxification-related gene families in T. infestans and Rhodnius prolixus. Conclusions and significance Our work contributes to the understanding of the detoxification response in vectors of Chagas disease. Given the absence of an annotated genome from T. infestans, the analysis presented here constitutes a resource for molecular and physiological studies in this species. The results increase the knowledge on detoxification processes in vectors of Chagas disease, and provide relevant information to explore undescribed potential insecticide resistance mechanisms in populations of these insects.
publishDate 2022
dc.date.none.fl_str_mv 2022-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189397
Traverso, Lucila María; Latorre Estivalis, Jose Manuel; Rocha Fernandes, Gabriel da; Fronza, Georgina; Lobbia, Patricia Alejandra; et al.; Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroids; Public Library of Science; Neglected Tropical Diseases; 16; 6; 6-2022; 1-27
1935-2735
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189397
identifier_str_mv Traverso, Lucila María; Latorre Estivalis, Jose Manuel; Rocha Fernandes, Gabriel da; Fronza, Georgina; Lobbia, Patricia Alejandra; et al.; Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroids; Public Library of Science; Neglected Tropical Diseases; 16; 6; 6-2022; 1-27
1935-2735
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0010060
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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