Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom

Autores
Leiva, Carlos Leónidas; Cangelosi, Adriana; Mariconda, Virginia; Celi Castillo, Ana Beatriz; Joaquim, Patricia Estefania; Geoghegan, Patricia; Fernandez Miyakawa, Mariano Enrique; Chacana, Pablo
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of egg yolk antibodies—IgY technology—represents an alternative to the production of mammalian immunoglobulins and has several advantages regarding animal welfare and lower costs of production. The use of adjuvants to achieve the hyperimmunization of laying hens plays a key role in the success of the production of high levels of the antibodies. In the present work, two different adjuvant systems (Freund’s adjuvants and MontanideTM ISA 71 VG) were compared to produce IgY anti-Bothrops alternatus. For the first immunization, formalin-inactivated Salmonella was added to MontanideTM ISA 71 VG to emulate Freund’s complete adjuvant which includes a mycobacteria antigen. After eight immunizations, IgY produced by using either adjuvant was able to neutralize the lethal activity of the venom in a mouse model, but differences were found regarding the recognition of components of the venom between the two adjuvants tested. Overall, MontanideTM adjuvant used in this work could be a good alternative choice to produce antibodies capable of neutralizing the lethality of complex antigens. This adjuvant is commercially available and used in the formulation of several poultry vaccines and could be used for the IgY technology instead of traditional immunomodulators such as Freund’s adjuvants.
Instituto de Patobiología
Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cangelosi, Adriana. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Mariconda, Virginia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Celi Castillo, Ana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Celi Castillo, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Joaquim, Patricia Estefania. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Joaquim, Patricia Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Geoghegan, Patricia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Fernandez Miyakawa, Mariano Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Applied Microbiology and Biotechnology (Published: 01 February 2023)
Materia
Egg Yolk
Adjuvants
Mass Spectrometry
Antibodies
Neutralization
Yema de Huevo
Coadyuvantes
Espectrometría de Masas
Anticuerpos
Neutralización
IgY Antibodies
Anticuerpos IgY
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/13956
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oai_identifier_str oai:localhost:20.500.12123/13956
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venomLeiva, Carlos LeónidasCangelosi, AdrianaMariconda, VirginiaCeli Castillo, Ana BeatrizJoaquim, Patricia EstefaniaGeoghegan, PatriciaFernandez Miyakawa, Mariano EnriqueChacana, PabloEgg YolkAdjuvantsMass SpectrometryAntibodiesNeutralizationYema de HuevoCoadyuvantesEspectrometría de MasasAnticuerposNeutralizaciónIgY AntibodiesAnticuerpos IgYThe use of egg yolk antibodies—IgY technology—represents an alternative to the production of mammalian immunoglobulins and has several advantages regarding animal welfare and lower costs of production. The use of adjuvants to achieve the hyperimmunization of laying hens plays a key role in the success of the production of high levels of the antibodies. In the present work, two different adjuvant systems (Freund’s adjuvants and MontanideTM ISA 71 VG) were compared to produce IgY anti-Bothrops alternatus. For the first immunization, formalin-inactivated Salmonella was added to MontanideTM ISA 71 VG to emulate Freund’s complete adjuvant which includes a mycobacteria antigen. After eight immunizations, IgY produced by using either adjuvant was able to neutralize the lethal activity of the venom in a mouse model, but differences were found regarding the recognition of components of the venom between the two adjuvants tested. Overall, MontanideTM adjuvant used in this work could be a good alternative choice to produce antibodies capable of neutralizing the lethality of complex antigens. This adjuvant is commercially available and used in the formulation of several poultry vaccines and could be used for the IgY technology instead of traditional immunomodulators such as Freund’s adjuvants.Instituto de PatobiologíaFil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cangelosi, Adriana. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; ArgentinaFil: Mariconda, Virginia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; ArgentinaFil: Celi Castillo, Ana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Celi Castillo, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Joaquim, Patricia Estefania. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Joaquim, Patricia Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Geoghegan, Patricia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; ArgentinaFil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Fernandez Miyakawa, Mariano Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2023-02-13T14:05:17Z2023-02-13T14:05:17Z2023-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13956https://link.springer.com/article/10.1007/s00253-023-12409-31432-06140175-7598https://doi.org/10.1007/s00253-023-12409-3Applied Microbiology and Biotechnology (Published: 01 February 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:44Zoai:localhost:20.500.12123/13956instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:44.398INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom
title Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom
spellingShingle Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom
Leiva, Carlos Leónidas
Egg Yolk
Adjuvants
Mass Spectrometry
Antibodies
Neutralization
Yema de Huevo
Coadyuvantes
Espectrometría de Masas
Anticuerpos
Neutralización
IgY Antibodies
Anticuerpos IgY
title_short Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom
title_full Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom
title_fullStr Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom
title_full_unstemmed Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom
title_sort Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom
dc.creator.none.fl_str_mv Leiva, Carlos Leónidas
Cangelosi, Adriana
Mariconda, Virginia
Celi Castillo, Ana Beatriz
Joaquim, Patricia Estefania
Geoghegan, Patricia
Fernandez Miyakawa, Mariano Enrique
Chacana, Pablo
author Leiva, Carlos Leónidas
author_facet Leiva, Carlos Leónidas
Cangelosi, Adriana
Mariconda, Virginia
Celi Castillo, Ana Beatriz
Joaquim, Patricia Estefania
Geoghegan, Patricia
Fernandez Miyakawa, Mariano Enrique
Chacana, Pablo
author_role author
author2 Cangelosi, Adriana
Mariconda, Virginia
Celi Castillo, Ana Beatriz
Joaquim, Patricia Estefania
Geoghegan, Patricia
Fernandez Miyakawa, Mariano Enrique
Chacana, Pablo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Egg Yolk
Adjuvants
Mass Spectrometry
Antibodies
Neutralization
Yema de Huevo
Coadyuvantes
Espectrometría de Masas
Anticuerpos
Neutralización
IgY Antibodies
Anticuerpos IgY
topic Egg Yolk
Adjuvants
Mass Spectrometry
Antibodies
Neutralization
Yema de Huevo
Coadyuvantes
Espectrometría de Masas
Anticuerpos
Neutralización
IgY Antibodies
Anticuerpos IgY
dc.description.none.fl_txt_mv The use of egg yolk antibodies—IgY technology—represents an alternative to the production of mammalian immunoglobulins and has several advantages regarding animal welfare and lower costs of production. The use of adjuvants to achieve the hyperimmunization of laying hens plays a key role in the success of the production of high levels of the antibodies. In the present work, two different adjuvant systems (Freund’s adjuvants and MontanideTM ISA 71 VG) were compared to produce IgY anti-Bothrops alternatus. For the first immunization, formalin-inactivated Salmonella was added to MontanideTM ISA 71 VG to emulate Freund’s complete adjuvant which includes a mycobacteria antigen. After eight immunizations, IgY produced by using either adjuvant was able to neutralize the lethal activity of the venom in a mouse model, but differences were found regarding the recognition of components of the venom between the two adjuvants tested. Overall, MontanideTM adjuvant used in this work could be a good alternative choice to produce antibodies capable of neutralizing the lethality of complex antigens. This adjuvant is commercially available and used in the formulation of several poultry vaccines and could be used for the IgY technology instead of traditional immunomodulators such as Freund’s adjuvants.
Instituto de Patobiología
Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cangelosi, Adriana. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Mariconda, Virginia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Celi Castillo, Ana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Celi Castillo, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Joaquim, Patricia Estefania. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Joaquim, Patricia Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Geoghegan, Patricia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina
Fil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Fernandez Miyakawa, Mariano Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The use of egg yolk antibodies—IgY technology—represents an alternative to the production of mammalian immunoglobulins and has several advantages regarding animal welfare and lower costs of production. The use of adjuvants to achieve the hyperimmunization of laying hens plays a key role in the success of the production of high levels of the antibodies. In the present work, two different adjuvant systems (Freund’s adjuvants and MontanideTM ISA 71 VG) were compared to produce IgY anti-Bothrops alternatus. For the first immunization, formalin-inactivated Salmonella was added to MontanideTM ISA 71 VG to emulate Freund’s complete adjuvant which includes a mycobacteria antigen. After eight immunizations, IgY produced by using either adjuvant was able to neutralize the lethal activity of the venom in a mouse model, but differences were found regarding the recognition of components of the venom between the two adjuvants tested. Overall, MontanideTM adjuvant used in this work could be a good alternative choice to produce antibodies capable of neutralizing the lethality of complex antigens. This adjuvant is commercially available and used in the formulation of several poultry vaccines and could be used for the IgY technology instead of traditional immunomodulators such as Freund’s adjuvants.
publishDate 2023
dc.date.none.fl_str_mv 2023-02-13T14:05:17Z
2023-02-13T14:05:17Z
2023-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13956
https://link.springer.com/article/10.1007/s00253-023-12409-3
1432-0614
0175-7598
https://doi.org/10.1007/s00253-023-12409-3
url http://hdl.handle.net/20.500.12123/13956
https://link.springer.com/article/10.1007/s00253-023-12409-3
https://doi.org/10.1007/s00253-023-12409-3
identifier_str_mv 1432-0614
0175-7598
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Applied Microbiology and Biotechnology (Published: 01 February 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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