Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens

Autores
Soria, Mario; Godano, Eduardo Ignacio; Leiva, Leonardo Esteban; Bueno, Dante Javier
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Our study was undertaken to determine the best samples and selective-differential plating media to be used for Salmonella spp. isolation. We also compared hematological and serum biochemical values, Salmonella biovar Gallinarum (SG) detection (isolation and serological test), and inflammatory intestinal response (fecal leukocyte) in laying hens with naturally occurring fowl typhoid outbreaks. Furthermore, we looked for a biomarker of SG infection. Spleen, liver, ovarian follicle content, and bone marrow were found to be the best samples for SG isolation and the agreement between MacConkey-Salmonella Shigella agar was slight to excellent. The laying hens with SG isolation and rapid serum plate agglutination positive results showed a higher percentage of heterophils, heterophil/lymphocyte ratio and total white blood cells, and a lower percentage of lymphocytes than those with negative results. Furthermore, the positive fecal leukocyte samples had a higher percentage of heterophils, gamma-glutamyl transferase, total protein and globulin values than negative samples. Five biomarkers’ cut-offs are proposed to distinguish between laying hens positive and negative to SG isolation.
EEA Concepción del Uruguay
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Soria, Mario Alberto. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, sede Basavilbaso; Argentina
Fil: Godano, Eduardo Ignacio. Tecnovo S.A., Entre Ríos; Argentina
Fil: Godano, Eduardo Ignacio. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Crespo. Instituto Tecnológico Universitario; Argentina
Fil: Leiva, Leonardo Esteban. Grupo Motta, Entre Ríos; Argentina
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, sede Basavilbaso; Argentina
Fuente
Comparative Immunology, Microbiology and Infectious Diseases 101 : 102055. (October 2023)
Materia
Salmonella
Layer Chickens
Animal Diseases
Salmonella gallinarum
Biochemical Analysis
Serology
Gallina Ponedora
Enfermedades de los Animales
Análisis Bioquímico
Serología
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hensSoria, MarioGodano, Eduardo IgnacioLeiva, Leonardo EstebanBueno, Dante JavierSalmonellaLayer ChickensAnimal DiseasesSalmonella gallinarumBiochemical AnalysisSerologyGallina PonedoraEnfermedades de los AnimalesAnálisis BioquímicoSerologíaOur study was undertaken to determine the best samples and selective-differential plating media to be used for Salmonella spp. isolation. We also compared hematological and serum biochemical values, Salmonella biovar Gallinarum (SG) detection (isolation and serological test), and inflammatory intestinal response (fecal leukocyte) in laying hens with naturally occurring fowl typhoid outbreaks. Furthermore, we looked for a biomarker of SG infection. Spleen, liver, ovarian follicle content, and bone marrow were found to be the best samples for SG isolation and the agreement between MacConkey-Salmonella Shigella agar was slight to excellent. The laying hens with SG isolation and rapid serum plate agglutination positive results showed a higher percentage of heterophils, heterophil/lymphocyte ratio and total white blood cells, and a lower percentage of lymphocytes than those with negative results. Furthermore, the positive fecal leukocyte samples had a higher percentage of heterophils, gamma-glutamyl transferase, total protein and globulin values than negative samples. Five biomarkers’ cut-offs are proposed to distinguish between laying hens positive and negative to SG isolation.EEA Concepción del UruguayFil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Soria, Mario Alberto. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, sede Basavilbaso; ArgentinaFil: Godano, Eduardo Ignacio. Tecnovo S.A., Entre Ríos; ArgentinaFil: Godano, Eduardo Ignacio. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Crespo. Instituto Tecnológico Universitario; ArgentinaFil: Leiva, Leonardo Esteban. Grupo Motta, Entre Ríos; ArgentinaFil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, sede Basavilbaso; ArgentinaElsevier2023-09-18T13:45:34Z2023-09-18T13:45:34Z2023-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15245https://www.sciencedirect.com/science/article/abs/pii/S01479571230011331878-16670147-9571https://doi.org/10.1016/j.cimid.2023.102055Comparative Immunology, Microbiology and Infectious Diseases 101 : 102055. (October 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:18:28Zoai:localhost:20.500.12123/15245instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:18:29.093INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens
title Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens
spellingShingle Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens
Soria, Mario
Salmonella
Layer Chickens
Animal Diseases
Salmonella gallinarum
Biochemical Analysis
Serology
Gallina Ponedora
Enfermedades de los Animales
Análisis Bioquímico
Serología
title_short Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens
title_full Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens
title_fullStr Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens
title_full_unstemmed Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens
title_sort Relationship of Salmonella isolation with different serum and inflammatory intestinal parameters in natural fowl typhoid outbreaks from laying hens
dc.creator.none.fl_str_mv Soria, Mario
Godano, Eduardo Ignacio
Leiva, Leonardo Esteban
Bueno, Dante Javier
author Soria, Mario
author_facet Soria, Mario
Godano, Eduardo Ignacio
Leiva, Leonardo Esteban
Bueno, Dante Javier
author_role author
author2 Godano, Eduardo Ignacio
Leiva, Leonardo Esteban
Bueno, Dante Javier
author2_role author
author
author
dc.subject.none.fl_str_mv Salmonella
Layer Chickens
Animal Diseases
Salmonella gallinarum
Biochemical Analysis
Serology
Gallina Ponedora
Enfermedades de los Animales
Análisis Bioquímico
Serología
topic Salmonella
Layer Chickens
Animal Diseases
Salmonella gallinarum
Biochemical Analysis
Serology
Gallina Ponedora
Enfermedades de los Animales
Análisis Bioquímico
Serología
dc.description.none.fl_txt_mv Our study was undertaken to determine the best samples and selective-differential plating media to be used for Salmonella spp. isolation. We also compared hematological and serum biochemical values, Salmonella biovar Gallinarum (SG) detection (isolation and serological test), and inflammatory intestinal response (fecal leukocyte) in laying hens with naturally occurring fowl typhoid outbreaks. Furthermore, we looked for a biomarker of SG infection. Spleen, liver, ovarian follicle content, and bone marrow were found to be the best samples for SG isolation and the agreement between MacConkey-Salmonella Shigella agar was slight to excellent. The laying hens with SG isolation and rapid serum plate agglutination positive results showed a higher percentage of heterophils, heterophil/lymphocyte ratio and total white blood cells, and a lower percentage of lymphocytes than those with negative results. Furthermore, the positive fecal leukocyte samples had a higher percentage of heterophils, gamma-glutamyl transferase, total protein and globulin values than negative samples. Five biomarkers’ cut-offs are proposed to distinguish between laying hens positive and negative to SG isolation.
EEA Concepción del Uruguay
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Soria, Mario Alberto. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, sede Basavilbaso; Argentina
Fil: Godano, Eduardo Ignacio. Tecnovo S.A., Entre Ríos; Argentina
Fil: Godano, Eduardo Ignacio. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Crespo. Instituto Tecnológico Universitario; Argentina
Fil: Leiva, Leonardo Esteban. Grupo Motta, Entre Ríos; Argentina
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, sede Basavilbaso; Argentina
description Our study was undertaken to determine the best samples and selective-differential plating media to be used for Salmonella spp. isolation. We also compared hematological and serum biochemical values, Salmonella biovar Gallinarum (SG) detection (isolation and serological test), and inflammatory intestinal response (fecal leukocyte) in laying hens with naturally occurring fowl typhoid outbreaks. Furthermore, we looked for a biomarker of SG infection. Spleen, liver, ovarian follicle content, and bone marrow were found to be the best samples for SG isolation and the agreement between MacConkey-Salmonella Shigella agar was slight to excellent. The laying hens with SG isolation and rapid serum plate agglutination positive results showed a higher percentage of heterophils, heterophil/lymphocyte ratio and total white blood cells, and a lower percentage of lymphocytes than those with negative results. Furthermore, the positive fecal leukocyte samples had a higher percentage of heterophils, gamma-glutamyl transferase, total protein and globulin values than negative samples. Five biomarkers’ cut-offs are proposed to distinguish between laying hens positive and negative to SG isolation.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-18T13:45:34Z
2023-09-18T13:45:34Z
2023-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15245
https://www.sciencedirect.com/science/article/abs/pii/S0147957123001133
1878-1667
0147-9571
https://doi.org/10.1016/j.cimid.2023.102055
url http://hdl.handle.net/20.500.12123/15245
https://www.sciencedirect.com/science/article/abs/pii/S0147957123001133
https://doi.org/10.1016/j.cimid.2023.102055
identifier_str_mv 1878-1667
0147-9571
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Comparative Immunology, Microbiology and Infectious Diseases 101 : 102055. (October 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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