Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs

Autores
Pérez-Iglesias, Juan Manuel; Natale, Guillermo S.; Brodeur, Celine Marie; Larramendy, Marcelo L.
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Imazethapyr, a post-emergent herbicide used in worldwide soybean and corn crops, induces genetic and biochemical alterations in aquatic vertebrates. This study examined the relationship between biomarkers at different organization levels and imazethapyr real-life route exposure in Boana pulchella adults. Frogs were exposed to imazethapyr-based formulation Pivot® H (10.59%) at concentrations representing possible acute routes: field runoff (S1:10 mg.L−1), exposure after direct foliar application (S2:100 mg.L−1) and during direct foliar application (S3:1000 mg.L−1). Post-exposure, endpoints levels were evaluated: organism alterations, biochemical activities and cytogenetic assays. Forty-eight hours post-exposure, antioxidant enzymes decrease, micronuclei induction and DNA damage were observed in all scenarios, while cholinesterase activity increase and body condition reduction were observed in frog-exposed to S3. Ninety-six hours postexposure, frogs showed glutathione-S-transferase inhibition in S1, micronuclei induction in S2 and S3, and DNA-damage increase in S3. Herbicides routes of exposures in real-life could indicate that authorized applications have a risk to amphibian populations.
Instituto de Recursos Biológicos
Fil: Pérez Iglesias, J.M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina.
Fil: Natale, G.S. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brodeur, Celine Marie Julie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Larramendy, Marcelo L. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Ecotoxicology 32 (2) : 1-12 (Published: 16 March 2023)
Materia
Rana
Fitotoxicidad
Phytotoxicity
Frogs
Pesticides
Plaguicidas
Biomarkers Integrative Responses
Dermal Exposure
Imazethapyr
Phisiological Stress
Respuestas Integradoras de Biomarcadores
Exposición Dérmica
Estrés Fisiológico
Boana pulchella
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/14340
Acceder
id INTADig_3421ddbca2a73971f065a6853d0af3de
oai_identifier_str oai:localhost:20.500.12123/14340
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult FrogsPérez-Iglesias, Juan ManuelNatale, Guillermo S.Brodeur, Celine MarieLarramendy, Marcelo L.RanaFitotoxicidadPhytotoxicityFrogsPesticidesPlaguicidasBiomarkers Integrative ResponsesDermal ExposureImazethapyrPhisiological StressRespuestas Integradoras de BiomarcadoresExposición DérmicaEstrés FisiológicoBoana pulchellaImazethapyr, a post-emergent herbicide used in worldwide soybean and corn crops, induces genetic and biochemical alterations in aquatic vertebrates. This study examined the relationship between biomarkers at different organization levels and imazethapyr real-life route exposure in Boana pulchella adults. Frogs were exposed to imazethapyr-based formulation Pivot® H (10.59%) at concentrations representing possible acute routes: field runoff (S1:10 mg.L−1), exposure after direct foliar application (S2:100 mg.L−1) and during direct foliar application (S3:1000 mg.L−1). Post-exposure, endpoints levels were evaluated: organism alterations, biochemical activities and cytogenetic assays. Forty-eight hours post-exposure, antioxidant enzymes decrease, micronuclei induction and DNA damage were observed in all scenarios, while cholinesterase activity increase and body condition reduction were observed in frog-exposed to S3. Ninety-six hours postexposure, frogs showed glutathione-S-transferase inhibition in S1, micronuclei induction in S2 and S3, and DNA-damage increase in S3. Herbicides routes of exposures in real-life could indicate that authorized applications have a risk to amphibian populations.Instituto de Recursos BiológicosFil: Pérez Iglesias, J.M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina.Fil: Natale, G.S. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brodeur, Celine Marie Julie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Larramendy, Marcelo L. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2023-03-28T17:29:38Z2023-03-28T17:29:38Z2023-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14340https://link.springer.com/article/10.1007/s10646-023-02639-61573-30170963-9292https://doi.org/10.1007/s10646-023-02639-6Ecotoxicology 32 (2) : 1-12 (Published: 16 March 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E2-I054-001/2019-PE-E2-I054-001/AR./Gestión sostenible de fitosanitariosinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:46Zoai:localhost:20.500.12123/14340instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:47.175INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs
title Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs
spellingShingle Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs
Pérez-Iglesias, Juan Manuel
Rana
Fitotoxicidad
Phytotoxicity
Frogs
Pesticides
Plaguicidas
Biomarkers Integrative Responses
Dermal Exposure
Imazethapyr
Phisiological Stress
Respuestas Integradoras de Biomarcadores
Exposición Dérmica
Estrés Fisiológico
Boana pulchella
title_short Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs
title_full Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs
title_fullStr Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs
title_full_unstemmed Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs
title_sort Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs
dc.creator.none.fl_str_mv Pérez-Iglesias, Juan Manuel
Natale, Guillermo S.
Brodeur, Celine Marie
Larramendy, Marcelo L.
author Pérez-Iglesias, Juan Manuel
author_facet Pérez-Iglesias, Juan Manuel
Natale, Guillermo S.
Brodeur, Celine Marie
Larramendy, Marcelo L.
author_role author
author2 Natale, Guillermo S.
Brodeur, Celine Marie
Larramendy, Marcelo L.
author2_role author
author
author
dc.subject.none.fl_str_mv Rana
Fitotoxicidad
Phytotoxicity
Frogs
Pesticides
Plaguicidas
Biomarkers Integrative Responses
Dermal Exposure
Imazethapyr
Phisiological Stress
Respuestas Integradoras de Biomarcadores
Exposición Dérmica
Estrés Fisiológico
Boana pulchella
topic Rana
Fitotoxicidad
Phytotoxicity
Frogs
Pesticides
Plaguicidas
Biomarkers Integrative Responses
Dermal Exposure
Imazethapyr
Phisiological Stress
Respuestas Integradoras de Biomarcadores
Exposición Dérmica
Estrés Fisiológico
Boana pulchella
dc.description.none.fl_txt_mv Imazethapyr, a post-emergent herbicide used in worldwide soybean and corn crops, induces genetic and biochemical alterations in aquatic vertebrates. This study examined the relationship between biomarkers at different organization levels and imazethapyr real-life route exposure in Boana pulchella adults. Frogs were exposed to imazethapyr-based formulation Pivot® H (10.59%) at concentrations representing possible acute routes: field runoff (S1:10 mg.L−1), exposure after direct foliar application (S2:100 mg.L−1) and during direct foliar application (S3:1000 mg.L−1). Post-exposure, endpoints levels were evaluated: organism alterations, biochemical activities and cytogenetic assays. Forty-eight hours post-exposure, antioxidant enzymes decrease, micronuclei induction and DNA damage were observed in all scenarios, while cholinesterase activity increase and body condition reduction were observed in frog-exposed to S3. Ninety-six hours postexposure, frogs showed glutathione-S-transferase inhibition in S1, micronuclei induction in S2 and S3, and DNA-damage increase in S3. Herbicides routes of exposures in real-life could indicate that authorized applications have a risk to amphibian populations.
Instituto de Recursos Biológicos
Fil: Pérez Iglesias, J.M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina.
Fil: Natale, G.S. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brodeur, Celine Marie Julie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Larramendy, Marcelo L. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Imazethapyr, a post-emergent herbicide used in worldwide soybean and corn crops, induces genetic and biochemical alterations in aquatic vertebrates. This study examined the relationship between biomarkers at different organization levels and imazethapyr real-life route exposure in Boana pulchella adults. Frogs were exposed to imazethapyr-based formulation Pivot® H (10.59%) at concentrations representing possible acute routes: field runoff (S1:10 mg.L−1), exposure after direct foliar application (S2:100 mg.L−1) and during direct foliar application (S3:1000 mg.L−1). Post-exposure, endpoints levels were evaluated: organism alterations, biochemical activities and cytogenetic assays. Forty-eight hours post-exposure, antioxidant enzymes decrease, micronuclei induction and DNA damage were observed in all scenarios, while cholinesterase activity increase and body condition reduction were observed in frog-exposed to S3. Ninety-six hours postexposure, frogs showed glutathione-S-transferase inhibition in S1, micronuclei induction in S2 and S3, and DNA-damage increase in S3. Herbicides routes of exposures in real-life could indicate that authorized applications have a risk to amphibian populations.
publishDate 2023
dc.date.none.fl_str_mv 2023-03-28T17:29:38Z
2023-03-28T17:29:38Z
2023-03-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14340
https://link.springer.com/article/10.1007/s10646-023-02639-6
1573-3017
0963-9292
https://doi.org/10.1007/s10646-023-02639-6
url http://hdl.handle.net/20.500.12123/14340
https://link.springer.com/article/10.1007/s10646-023-02639-6
https://doi.org/10.1007/s10646-023-02639-6
identifier_str_mv 1573-3017
0963-9292
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E2-I054-001/2019-PE-E2-I054-001/AR./Gestión sostenible de fitosanitarios
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Ecotoxicology 32 (2) : 1-12 (Published: 16 March 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341409607122944
score 12.623145

Publicaciones similares