Multiple Level Effects of Imazethapyr on Leptodactylus latinasus (Anura) Adult Frogs

Autores
Pérez-Iglesias, Juan Manuel; Fanali, L.Z.; Franco-Belussi, L.; Natale, Guillermo Sebastián; De Oliveira, C.; Brodeur, Celine Marie; Larramendy, Marcelo L.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Imazethapyr is an herbicide that is used in a variety of crops worldwide, including soybean and corn. The aim of the present study was to evaluate the biomarkers responses of adult Leptodactylus latinasus exposed to the formulation Pivot® H (10.59% imazethapyr) in the laboratory at concentrations and under conditions that simulate two potential field exposure scenarios: an immersion in field runoff (Scenario 1: 10 mg/L) and a direct exposure to the droplets emitted by spray noozles (Scenario 2: 1000 mg/L). In both scenarios, the experimental procedure involved completely immersing the frogs over a period of 15 s. Different endpoints were evaluated at several ecotoxicological levels 48 and 96 h after the herbicide exposure. These included individual (biometric indices and behavior alterations), histological (liver pigments and lesions), biochemical (catalase, glutathione system and cholinesterase activities) and genotoxic effects (micronuclei induction and nuclear abnormalities). Forty-eight hours after imazethapyr exposure, frogs submitted to Scenario 1 presented an inhibition of liver glutathione-S-transferase activity, whereas histological alterations and increased hepatic cholinesterase levels were observed in frogs exposed under Scenario 2. Ninety-six hours after exposure to the imazethapyr formulation, frogs from the Scenario 1 treatment presented a decrease in liver melanin and hemosiderin, increased hepatic catalase activity and micronuclei induction. For their part, frogs exposed to Scenario 2 presented a decrease in the hepatosomatic index, an increase in liver alterations, melanin reduction and micronuclei induction. The multivariate analysis enables correlations to be made between biomarkers of different organizational level in exposed anurans. Our result indicates that real exposure to imazethapyr formulations under field conditions may pose a risk to Leptodactylus latinasus populations living in the agroecosystems.
Instituto de Recursos Biológicos
Fil: Pérez-Iglesias, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fil: Pérez-Iglesias, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fil: Pérez-Iglesias, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Pérez-Iglesias, Juan Manuel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Pérez-Iglesias, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; Argentina
Fil: Fanali, L.Z. Universidade Estadual Paulista. Departamento de Biologia; Brasil
Fil: Franco-Belussi, L. Universidade Estadual Paulista. Departamento de Biologia; Brasil
Fil: Franco-Belussi, L. Universidade Federal de Mato Grosso do Sul. Instituto de Biociências (INBIO). Laboratorio de Patologia Experimental (LAPEx); Brasil
Fil: Natale, Guillermo S. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente; Argentina
Fil: Natale, Guillermo S. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fil: De Oliveira, C. Universidade Estadual Paulista. Departamento de Biologia; Brasil
Fil: Brodeur, Celine Marie Julie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Bilológicos; Argentina
Fil: Brodeur, Celine Marie Julie. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Larramendy, Marcelo L. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Larramendy, Marcelo L. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
Fuente
Archives of Environmental Contamination and Toxicology (Published: 18 August 2021)
Materia
Herbicidas
Leptodactylidae
Rana
Agroecosistemas
Herbicides
Frogs
Agroecosystems
Anura
Imazethapyr
Leptodactylus latinasus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10369
Acceder
id INTADig_f09d2a3cc89abd254e388e720e89b426
oai_identifier_str oai:localhost:20.500.12123/10369
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Multiple Level Effects of Imazethapyr on Leptodactylus latinasus (Anura) Adult FrogsPérez-Iglesias, Juan ManuelFanali, L.Z.Franco-Belussi, L.Natale, Guillermo SebastiánDe Oliveira, C.Brodeur, Celine MarieLarramendy, Marcelo L.HerbicidasLeptodactylidaeRanaAgroecosistemasHerbicidesFrogsAgroecosystemsAnuraImazethapyrLeptodactylus latinasusImazethapyr is an herbicide that is used in a variety of crops worldwide, including soybean and corn. The aim of the present study was to evaluate the biomarkers responses of adult Leptodactylus latinasus exposed to the formulation Pivot® H (10.59% imazethapyr) in the laboratory at concentrations and under conditions that simulate two potential field exposure scenarios: an immersion in field runoff (Scenario 1: 10 mg/L) and a direct exposure to the droplets emitted by spray noozles (Scenario 2: 1000 mg/L). In both scenarios, the experimental procedure involved completely immersing the frogs over a period of 15 s. Different endpoints were evaluated at several ecotoxicological levels 48 and 96 h after the herbicide exposure. These included individual (biometric indices and behavior alterations), histological (liver pigments and lesions), biochemical (catalase, glutathione system and cholinesterase activities) and genotoxic effects (micronuclei induction and nuclear abnormalities). Forty-eight hours after imazethapyr exposure, frogs submitted to Scenario 1 presented an inhibition of liver glutathione-S-transferase activity, whereas histological alterations and increased hepatic cholinesterase levels were observed in frogs exposed under Scenario 2. Ninety-six hours after exposure to the imazethapyr formulation, frogs from the Scenario 1 treatment presented a decrease in liver melanin and hemosiderin, increased hepatic catalase activity and micronuclei induction. For their part, frogs exposed to Scenario 2 presented a decrease in the hepatosomatic index, an increase in liver alterations, melanin reduction and micronuclei induction. The multivariate analysis enables correlations to be made between biomarkers of different organizational level in exposed anurans. Our result indicates that real exposure to imazethapyr formulations under field conditions may pose a risk to Leptodactylus latinasus populations living in the agroecosystems.Instituto de Recursos BiológicosFil: Pérez-Iglesias, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente (CIM); ArgentinaFil: Pérez-Iglesias, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); ArgentinaFil: Pérez-Iglesias, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaFil: Pérez-Iglesias, Juan Manuel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Pérez-Iglesias, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; ArgentinaFil: Fanali, L.Z. Universidade Estadual Paulista. Departamento de Biologia; BrasilFil: Franco-Belussi, L. Universidade Estadual Paulista. Departamento de Biologia; BrasilFil: Franco-Belussi, L. Universidade Federal de Mato Grosso do Sul. Instituto de Biociências (INBIO). Laboratorio de Patologia Experimental (LAPEx); BrasilFil: Natale, Guillermo S. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente; ArgentinaFil: Natale, Guillermo S. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); ArgentinaFil: De Oliveira, C. Universidade Estadual Paulista. Departamento de Biologia; BrasilFil: Brodeur, Celine Marie Julie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Bilológicos; ArgentinaFil: Brodeur, Celine Marie Julie. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Larramendy, Marcelo L. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaFil: Larramendy, Marcelo L. Consejo Nacional de Investigaciones Científicas y Técnicas, ArgentinaSpringer2021-09-27T14:57:21Z2021-09-27T14:57:21Z2021-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10369https://link.springer.com/article/10.1007/s00244-021-00880-w0090-43411432-0703https://doi.org/10.1007/s00244-021-00880-wArchives of Environmental Contamination and Toxicology (Published: 18 August 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:49:07Zoai:localhost:20.500.12123/10369instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:07.964INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Multiple Level Effects of Imazethapyr on Leptodactylus latinasus (Anura) Adult Frogs
title Multiple Level Effects of Imazethapyr on Leptodactylus latinasus (Anura) Adult Frogs
spellingShingle Multiple Level Effects of Imazethapyr on Leptodactylus latinasus (Anura) Adult Frogs
Pérez-Iglesias, Juan Manuel
Herbicidas
Leptodactylidae
Rana
Agroecosistemas
Herbicides
Frogs
Agroecosystems
Anura
Imazethapyr
Leptodactylus latinasus
title_short Multiple Level Effects of Imazethapyr on Leptodactylus latinasus (Anura) Adult Frogs
title_full Multiple Level Effects of Imazethapyr on Leptodactylus latinasus (Anura) Adult Frogs
title_fullStr Multiple Level Effects of Imazethapyr on Leptodactylus latinasus (Anura) Adult Frogs
title_full_unstemmed Multiple Level Effects of Imazethapyr on Leptodactylus latinasus (Anura) Adult Frogs
title_sort Multiple Level Effects of Imazethapyr on Leptodactylus latinasus (Anura) Adult Frogs
dc.creator.none.fl_str_mv Pérez-Iglesias, Juan Manuel
Fanali, L.Z.
Franco-Belussi, L.
Natale, Guillermo Sebastián
De Oliveira, C.
Brodeur, Celine Marie
Larramendy, Marcelo L.
author Pérez-Iglesias, Juan Manuel
author_facet Pérez-Iglesias, Juan Manuel
Fanali, L.Z.
Franco-Belussi, L.
Natale, Guillermo Sebastián
De Oliveira, C.
Brodeur, Celine Marie
Larramendy, Marcelo L.
author_role author
author2 Fanali, L.Z.
Franco-Belussi, L.
Natale, Guillermo Sebastián
De Oliveira, C.
Brodeur, Celine Marie
Larramendy, Marcelo L.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Herbicidas
Leptodactylidae
Rana
Agroecosistemas
Herbicides
Frogs
Agroecosystems
Anura
Imazethapyr
Leptodactylus latinasus
topic Herbicidas
Leptodactylidae
Rana
Agroecosistemas
Herbicides
Frogs
Agroecosystems
Anura
Imazethapyr
Leptodactylus latinasus
dc.description.none.fl_txt_mv Imazethapyr is an herbicide that is used in a variety of crops worldwide, including soybean and corn. The aim of the present study was to evaluate the biomarkers responses of adult Leptodactylus latinasus exposed to the formulation Pivot® H (10.59% imazethapyr) in the laboratory at concentrations and under conditions that simulate two potential field exposure scenarios: an immersion in field runoff (Scenario 1: 10 mg/L) and a direct exposure to the droplets emitted by spray noozles (Scenario 2: 1000 mg/L). In both scenarios, the experimental procedure involved completely immersing the frogs over a period of 15 s. Different endpoints were evaluated at several ecotoxicological levels 48 and 96 h after the herbicide exposure. These included individual (biometric indices and behavior alterations), histological (liver pigments and lesions), biochemical (catalase, glutathione system and cholinesterase activities) and genotoxic effects (micronuclei induction and nuclear abnormalities). Forty-eight hours after imazethapyr exposure, frogs submitted to Scenario 1 presented an inhibition of liver glutathione-S-transferase activity, whereas histological alterations and increased hepatic cholinesterase levels were observed in frogs exposed under Scenario 2. Ninety-six hours after exposure to the imazethapyr formulation, frogs from the Scenario 1 treatment presented a decrease in liver melanin and hemosiderin, increased hepatic catalase activity and micronuclei induction. For their part, frogs exposed to Scenario 2 presented a decrease in the hepatosomatic index, an increase in liver alterations, melanin reduction and micronuclei induction. The multivariate analysis enables correlations to be made between biomarkers of different organizational level in exposed anurans. Our result indicates that real exposure to imazethapyr formulations under field conditions may pose a risk to Leptodactylus latinasus populations living in the agroecosystems.
Instituto de Recursos Biológicos
Fil: Pérez-Iglesias, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fil: Pérez-Iglesias, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fil: Pérez-Iglesias, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Pérez-Iglesias, Juan Manuel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Pérez-Iglesias, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; Argentina
Fil: Fanali, L.Z. Universidade Estadual Paulista. Departamento de Biologia; Brasil
Fil: Franco-Belussi, L. Universidade Estadual Paulista. Departamento de Biologia; Brasil
Fil: Franco-Belussi, L. Universidade Federal de Mato Grosso do Sul. Instituto de Biociências (INBIO). Laboratorio de Patologia Experimental (LAPEx); Brasil
Fil: Natale, Guillermo S. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente; Argentina
Fil: Natale, Guillermo S. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fil: De Oliveira, C. Universidade Estadual Paulista. Departamento de Biologia; Brasil
Fil: Brodeur, Celine Marie Julie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Bilológicos; Argentina
Fil: Brodeur, Celine Marie Julie. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Larramendy, Marcelo L. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Larramendy, Marcelo L. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
description Imazethapyr is an herbicide that is used in a variety of crops worldwide, including soybean and corn. The aim of the present study was to evaluate the biomarkers responses of adult Leptodactylus latinasus exposed to the formulation Pivot® H (10.59% imazethapyr) in the laboratory at concentrations and under conditions that simulate two potential field exposure scenarios: an immersion in field runoff (Scenario 1: 10 mg/L) and a direct exposure to the droplets emitted by spray noozles (Scenario 2: 1000 mg/L). In both scenarios, the experimental procedure involved completely immersing the frogs over a period of 15 s. Different endpoints were evaluated at several ecotoxicological levels 48 and 96 h after the herbicide exposure. These included individual (biometric indices and behavior alterations), histological (liver pigments and lesions), biochemical (catalase, glutathione system and cholinesterase activities) and genotoxic effects (micronuclei induction and nuclear abnormalities). Forty-eight hours after imazethapyr exposure, frogs submitted to Scenario 1 presented an inhibition of liver glutathione-S-transferase activity, whereas histological alterations and increased hepatic cholinesterase levels were observed in frogs exposed under Scenario 2. Ninety-six hours after exposure to the imazethapyr formulation, frogs from the Scenario 1 treatment presented a decrease in liver melanin and hemosiderin, increased hepatic catalase activity and micronuclei induction. For their part, frogs exposed to Scenario 2 presented a decrease in the hepatosomatic index, an increase in liver alterations, melanin reduction and micronuclei induction. The multivariate analysis enables correlations to be made between biomarkers of different organizational level in exposed anurans. Our result indicates that real exposure to imazethapyr formulations under field conditions may pose a risk to Leptodactylus latinasus populations living in the agroecosystems.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-27T14:57:21Z
2021-09-27T14:57:21Z
2021-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10369
https://link.springer.com/article/10.1007/s00244-021-00880-w
0090-4341
1432-0703
https://doi.org/10.1007/s00244-021-00880-w
url http://hdl.handle.net/20.500.12123/10369
https://link.springer.com/article/10.1007/s00244-021-00880-w
https://doi.org/10.1007/s00244-021-00880-w
identifier_str_mv 0090-4341
1432-0703
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Archives of Environmental Contamination and Toxicology (Published: 18 August 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341391031599104
score 12.623145

Publicaciones similares