Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbanc...

Autores
Bahl, María Florencia; Salgado Costa, Carolina; Demetrio, Pablo Martín; Mac Loughlin, Tomás Mariano; Arruti, María Emilia; Brodeur, Celine Marie; Natale, Guillermo Sebastián
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Amphibians are the most threatened group of vertebrates because they have certain biological and ecological characteristics that make them sensitive to environmental changes. The aim of this study was to evaluate the health status of field-collected adult frogs of Leptodactylus luctator (Amphibia, Anura) living in sites with different anthropogenic disturbances (florihorticulture, petrochemical industry and sewage discharges) and a reference site without any detectable influence of such activities. To this end, a battery of 21 biomarkers (hematological, biochemical and individual biomarkers) was studied using a multivariate approach that allows us to evaluate the relationship between them and provide information on their usefulness. The frogs at the florihorticulture, petrochemical and sewage discharges sites exhibited several biomarkers far from homeostasis. In addition, we identified 11 of 21 biomarkers that were useful indicators of the health status of the frogs and allowed discrimination between study sites in the following order: lymphocytes (98 %), neutrophils (45 %), hemoglobin (42 %), monocytes (41 %), fat body index (35 %), eosinophils (35 %), hepatosomatic index (33 %), mean corpuscular hemoglobin (32 %), thrombocytes (27 %), catalase in liver (26 %), and GST in liver (26 %). The results suggest that hematological biomarkers contribute the most to site separation, whereas biochemical biomarkers contribute the least. The integral interpretation of the results also allowed us to diagnose the different health status of L. luctator: The frogs from the petrochemical industry were the most negatively affected, followed by the frogs from the sewages discharges and finally the frogs from the florihorticulture and reference sites. This is the first field study with anurans in which so many biomarkers were examined.
Instituto de Recursos Biológicos
Fil: Bahl, M.F. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fil: Salgado Costa, C. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fil: Demetrio, P.M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fil: Mac Loughlin, T.M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fil: Arruti, M.E. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fil: Brodeur, Celine Marie Julie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Natale, G.S. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fuente
Science of The Total Environment 933 : 173174. (July 2024)
Materia
Rana
Anfibio
Contaminación
Medio Ambiente
Frogs
Amphibians
Contamination
Environment
Biomarcadores
Biomarkers
Leptodactylus luctator
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/18404
Acceder
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oai_identifier_str oai:localhost:20.500.12123/18404
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network_name_str INTA Digital (INTA)
spelling Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbancesBahl, María FlorenciaSalgado Costa, CarolinaDemetrio, Pablo MartínMac Loughlin, Tomás MarianoArruti, María EmiliaBrodeur, Celine MarieNatale, Guillermo SebastiánRanaAnfibioContaminaciónMedio AmbienteFrogsAmphibiansContaminationEnvironmentBiomarcadoresBiomarkersLeptodactylus luctatorAmphibians are the most threatened group of vertebrates because they have certain biological and ecological characteristics that make them sensitive to environmental changes. The aim of this study was to evaluate the health status of field-collected adult frogs of Leptodactylus luctator (Amphibia, Anura) living in sites with different anthropogenic disturbances (florihorticulture, petrochemical industry and sewage discharges) and a reference site without any detectable influence of such activities. To this end, a battery of 21 biomarkers (hematological, biochemical and individual biomarkers) was studied using a multivariate approach that allows us to evaluate the relationship between them and provide information on their usefulness. The frogs at the florihorticulture, petrochemical and sewage discharges sites exhibited several biomarkers far from homeostasis. In addition, we identified 11 of 21 biomarkers that were useful indicators of the health status of the frogs and allowed discrimination between study sites in the following order: lymphocytes (98 %), neutrophils (45 %), hemoglobin (42 %), monocytes (41 %), fat body index (35 %), eosinophils (35 %), hepatosomatic index (33 %), mean corpuscular hemoglobin (32 %), thrombocytes (27 %), catalase in liver (26 %), and GST in liver (26 %). The results suggest that hematological biomarkers contribute the most to site separation, whereas biochemical biomarkers contribute the least. The integral interpretation of the results also allowed us to diagnose the different health status of L. luctator: The frogs from the petrochemical industry were the most negatively affected, followed by the frogs from the sewages discharges and finally the frogs from the florihorticulture and reference sites. This is the first field study with anurans in which so many biomarkers were examined.Instituto de Recursos BiológicosFil: Bahl, M.F. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); ArgentinaFil: Salgado Costa, C. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); ArgentinaFil: Demetrio, P.M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); ArgentinaFil: Mac Loughlin, T.M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); ArgentinaFil: Arruti, M.E. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); ArgentinaFil: Brodeur, Celine Marie Julie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Natale, G.S. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); ArgentinaElsevier2024-07-05T13:52:30Z2024-07-05T13:52:30Z2024-07-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/18404https://www.sciencedirect.com/science/article/abs/pii/S00489697240332170048-9697https://doi.org/10.1016/j.scitotenv.2024.173174Science of The Total Environment 933 : 173174. (July 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:29Zoai:localhost:20.500.12123/18404instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:29.957INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbances
title Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbances
spellingShingle Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbances
Bahl, María Florencia
Rana
Anfibio
Contaminación
Medio Ambiente
Frogs
Amphibians
Contamination
Environment
Biomarcadores
Biomarkers
Leptodactylus luctator
title_short Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbances
title_full Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbances
title_fullStr Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbances
title_full_unstemmed Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbances
title_sort Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbances
dc.creator.none.fl_str_mv Bahl, María Florencia
Salgado Costa, Carolina
Demetrio, Pablo Martín
Mac Loughlin, Tomás Mariano
Arruti, María Emilia
Brodeur, Celine Marie
Natale, Guillermo Sebastián
author Bahl, María Florencia
author_facet Bahl, María Florencia
Salgado Costa, Carolina
Demetrio, Pablo Martín
Mac Loughlin, Tomás Mariano
Arruti, María Emilia
Brodeur, Celine Marie
Natale, Guillermo Sebastián
author_role author
author2 Salgado Costa, Carolina
Demetrio, Pablo Martín
Mac Loughlin, Tomás Mariano
Arruti, María Emilia
Brodeur, Celine Marie
Natale, Guillermo Sebastián
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Rana
Anfibio
Contaminación
Medio Ambiente
Frogs
Amphibians
Contamination
Environment
Biomarcadores
Biomarkers
Leptodactylus luctator
topic Rana
Anfibio
Contaminación
Medio Ambiente
Frogs
Amphibians
Contamination
Environment
Biomarcadores
Biomarkers
Leptodactylus luctator
dc.description.none.fl_txt_mv Amphibians are the most threatened group of vertebrates because they have certain biological and ecological characteristics that make them sensitive to environmental changes. The aim of this study was to evaluate the health status of field-collected adult frogs of Leptodactylus luctator (Amphibia, Anura) living in sites with different anthropogenic disturbances (florihorticulture, petrochemical industry and sewage discharges) and a reference site without any detectable influence of such activities. To this end, a battery of 21 biomarkers (hematological, biochemical and individual biomarkers) was studied using a multivariate approach that allows us to evaluate the relationship between them and provide information on their usefulness. The frogs at the florihorticulture, petrochemical and sewage discharges sites exhibited several biomarkers far from homeostasis. In addition, we identified 11 of 21 biomarkers that were useful indicators of the health status of the frogs and allowed discrimination between study sites in the following order: lymphocytes (98 %), neutrophils (45 %), hemoglobin (42 %), monocytes (41 %), fat body index (35 %), eosinophils (35 %), hepatosomatic index (33 %), mean corpuscular hemoglobin (32 %), thrombocytes (27 %), catalase in liver (26 %), and GST in liver (26 %). The results suggest that hematological biomarkers contribute the most to site separation, whereas biochemical biomarkers contribute the least. The integral interpretation of the results also allowed us to diagnose the different health status of L. luctator: The frogs from the petrochemical industry were the most negatively affected, followed by the frogs from the sewages discharges and finally the frogs from the florihorticulture and reference sites. This is the first field study with anurans in which so many biomarkers were examined.
Instituto de Recursos Biológicos
Fil: Bahl, M.F. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fil: Salgado Costa, C. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fil: Demetrio, P.M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fil: Mac Loughlin, T.M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fil: Arruti, M.E. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
Fil: Brodeur, Celine Marie Julie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Natale, G.S. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina
description Amphibians are the most threatened group of vertebrates because they have certain biological and ecological characteristics that make them sensitive to environmental changes. The aim of this study was to evaluate the health status of field-collected adult frogs of Leptodactylus luctator (Amphibia, Anura) living in sites with different anthropogenic disturbances (florihorticulture, petrochemical industry and sewage discharges) and a reference site without any detectable influence of such activities. To this end, a battery of 21 biomarkers (hematological, biochemical and individual biomarkers) was studied using a multivariate approach that allows us to evaluate the relationship between them and provide information on their usefulness. The frogs at the florihorticulture, petrochemical and sewage discharges sites exhibited several biomarkers far from homeostasis. In addition, we identified 11 of 21 biomarkers that were useful indicators of the health status of the frogs and allowed discrimination between study sites in the following order: lymphocytes (98 %), neutrophils (45 %), hemoglobin (42 %), monocytes (41 %), fat body index (35 %), eosinophils (35 %), hepatosomatic index (33 %), mean corpuscular hemoglobin (32 %), thrombocytes (27 %), catalase in liver (26 %), and GST in liver (26 %). The results suggest that hematological biomarkers contribute the most to site separation, whereas biochemical biomarkers contribute the least. The integral interpretation of the results also allowed us to diagnose the different health status of L. luctator: The frogs from the petrochemical industry were the most negatively affected, followed by the frogs from the sewages discharges and finally the frogs from the florihorticulture and reference sites. This is the first field study with anurans in which so many biomarkers were examined.
publishDate 2024
dc.date.none.fl_str_mv 2024-07-05T13:52:30Z
2024-07-05T13:52:30Z
2024-07-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/18404
https://www.sciencedirect.com/science/article/abs/pii/S0048969724033217
0048-9697
https://doi.org/10.1016/j.scitotenv.2024.173174
url http://hdl.handle.net/20.500.12123/18404
https://www.sciencedirect.com/science/article/abs/pii/S0048969724033217
https://doi.org/10.1016/j.scitotenv.2024.173174
identifier_str_mv 0048-9697
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Science of The Total Environment 933 : 173174. (July 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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