Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑...

Autores
Moyano, Roberto Damián; Imperiale, Belen Rocio; Romero, Magalí; Santangelo, María De La Paz; Alvarado Pinedo, Maria Fiorella; Travería, Gabriel; Romano, Maria Isabel
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Paratuberculosis is an enteric disease caused by Mycobacterium avium sp. paratuberculosis (MAP) that affects mainly ruminant producing losses to the livestock industry. Many molecular epidemiological methods have been used to discriminate MAP isolates. Method: The aim of this study was to describe the genetic diversity of the Argentinean MAP isolates using a combination of two molecular systems, the mycobacterial interspersed repetitive unit–variable number tandem repeat (MIRU-VNTR) (“automated and “non-automated”) and the multi-locus short-sequence repeat (MLSSR) system. Results: Thirty-two isolates were identified as MAP of C type by IS900 polymerase chain reaction (PCA) and IS1311 PCA-restriction enzyme analysis. The main patterns found by both MIRU-VNTR systems were INMV1 (54.5%), INMV2 (24.2%) and INMV11 (9.1%). The INMV5, INMV8 and INMV16 were represented with one isolate each (3.0%). Only 4 MIRU-VNTR loci were polymorphic. Conclusion: Those isolates sharing the same INMV patterns were analyzed by MLSSR, being locus 2 the most polymorphic one showing isolates with 9, 10, 11, and more than 11 “G” repeats. Besides, the global discriminatory power among isolates could be increased using both techniques. Based on these results, a short version of the “automated” MIRU-VNTR could be used as a screening tool to group isolates genetically related and subsequently perform the SSR using locus 2 on those isolates sharing the same INMV pattern.
Instituto de Biotecnología
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Imperiale, Belen Rocio. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX); Argentina
Fil: Imperiale, Belen Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Magalí. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria (CEDIVE); Argentina
Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Santangelo, María De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarado Pinedo, Maria Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria (CEDIVE); Argentina
Fil: Travería, Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria (CEDIVE); Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
International Journal of Mycobacteriology 10 (1) : 51-59 (Marzo 2021)
Materia
Mycobacterium avium subsp. paratuberculosis
Infección por Mycobacterium avium
Variación Genética
Loci de Rasgos Cuantitativos
Aislamiento Genético
Mycobacterium avium Infections
Genetic Variation
Quantitative Trait Loci
Genetic Isolation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9081
Acceder
id INTADig_336e7ccd908b2fbf96c726a6ec327956
oai_identifier_str oai:localhost:20.500.12123/9081
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolatesMoyano, Roberto DamiánImperiale, Belen RocioRomero, MagalíSantangelo, María De La PazAlvarado Pinedo, Maria FiorellaTravería, GabrielRomano, Maria IsabelMycobacterium avium subsp. paratuberculosisInfección por Mycobacterium aviumVariación GenéticaLoci de Rasgos CuantitativosAislamiento GenéticoMycobacterium avium InfectionsGenetic VariationQuantitative Trait LociGenetic IsolationBackground: Paratuberculosis is an enteric disease caused by Mycobacterium avium sp. paratuberculosis (MAP) that affects mainly ruminant producing losses to the livestock industry. Many molecular epidemiological methods have been used to discriminate MAP isolates. Method: The aim of this study was to describe the genetic diversity of the Argentinean MAP isolates using a combination of two molecular systems, the mycobacterial interspersed repetitive unit–variable number tandem repeat (MIRU-VNTR) (“automated and “non-automated”) and the multi-locus short-sequence repeat (MLSSR) system. Results: Thirty-two isolates were identified as MAP of C type by IS900 polymerase chain reaction (PCA) and IS1311 PCA-restriction enzyme analysis. The main patterns found by both MIRU-VNTR systems were INMV1 (54.5%), INMV2 (24.2%) and INMV11 (9.1%). The INMV5, INMV8 and INMV16 were represented with one isolate each (3.0%). Only 4 MIRU-VNTR loci were polymorphic. Conclusion: Those isolates sharing the same INMV patterns were analyzed by MLSSR, being locus 2 the most polymorphic one showing isolates with 9, 10, 11, and more than 11 “G” repeats. Besides, the global discriminatory power among isolates could be increased using both techniques. Based on these results, a short version of the “automated” MIRU-VNTR could be used as a screening tool to group isolates genetically related and subsequently perform the SSR using locus 2 on those isolates sharing the same INMV pattern.Instituto de BiotecnologíaFil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Imperiale, Belen Rocio. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX); ArgentinaFil: Imperiale, Belen Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romero, Magalí. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria (CEDIVE); ArgentinaFil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Santangelo, María De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alvarado Pinedo, Maria Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria (CEDIVE); ArgentinaFil: Travería, Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria (CEDIVE); ArgentinaFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWolters Kluwer Medknow2021-04-14T10:45:47Z2021-04-14T10:45:47Z2021-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9081https://www.ijmyco.org/article.asp?issn=2212-5531;year=2021;volume=10;issue=1;spage=51;epage=59;aulast=Moyano2212-5531https://doi.org/10.4103/ijmy.ijmy_229_20International Journal of Mycobacteriology 10 (1) : 51-59 (Marzo 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:48:49Zoai:localhost:20.500.12123/9081instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:50.188INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates
title Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates
spellingShingle Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates
Moyano, Roberto Damián
Mycobacterium avium subsp. paratuberculosis
Infección por Mycobacterium avium
Variación Genética
Loci de Rasgos Cuantitativos
Aislamiento Genético
Mycobacterium avium Infections
Genetic Variation
Quantitative Trait Loci
Genetic Isolation
title_short Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates
title_full Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates
title_fullStr Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates
title_full_unstemmed Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates
title_sort Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates
dc.creator.none.fl_str_mv Moyano, Roberto Damián
Imperiale, Belen Rocio
Romero, Magalí
Santangelo, María De La Paz
Alvarado Pinedo, Maria Fiorella
Travería, Gabriel
Romano, Maria Isabel
author Moyano, Roberto Damián
author_facet Moyano, Roberto Damián
Imperiale, Belen Rocio
Romero, Magalí
Santangelo, María De La Paz
Alvarado Pinedo, Maria Fiorella
Travería, Gabriel
Romano, Maria Isabel
author_role author
author2 Imperiale, Belen Rocio
Romero, Magalí
Santangelo, María De La Paz
Alvarado Pinedo, Maria Fiorella
Travería, Gabriel
Romano, Maria Isabel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Mycobacterium avium subsp. paratuberculosis
Infección por Mycobacterium avium
Variación Genética
Loci de Rasgos Cuantitativos
Aislamiento Genético
Mycobacterium avium Infections
Genetic Variation
Quantitative Trait Loci
Genetic Isolation
topic Mycobacterium avium subsp. paratuberculosis
Infección por Mycobacterium avium
Variación Genética
Loci de Rasgos Cuantitativos
Aislamiento Genético
Mycobacterium avium Infections
Genetic Variation
Quantitative Trait Loci
Genetic Isolation
dc.description.none.fl_txt_mv Background: Paratuberculosis is an enteric disease caused by Mycobacterium avium sp. paratuberculosis (MAP) that affects mainly ruminant producing losses to the livestock industry. Many molecular epidemiological methods have been used to discriminate MAP isolates. Method: The aim of this study was to describe the genetic diversity of the Argentinean MAP isolates using a combination of two molecular systems, the mycobacterial interspersed repetitive unit–variable number tandem repeat (MIRU-VNTR) (“automated and “non-automated”) and the multi-locus short-sequence repeat (MLSSR) system. Results: Thirty-two isolates were identified as MAP of C type by IS900 polymerase chain reaction (PCA) and IS1311 PCA-restriction enzyme analysis. The main patterns found by both MIRU-VNTR systems were INMV1 (54.5%), INMV2 (24.2%) and INMV11 (9.1%). The INMV5, INMV8 and INMV16 were represented with one isolate each (3.0%). Only 4 MIRU-VNTR loci were polymorphic. Conclusion: Those isolates sharing the same INMV patterns were analyzed by MLSSR, being locus 2 the most polymorphic one showing isolates with 9, 10, 11, and more than 11 “G” repeats. Besides, the global discriminatory power among isolates could be increased using both techniques. Based on these results, a short version of the “automated” MIRU-VNTR could be used as a screening tool to group isolates genetically related and subsequently perform the SSR using locus 2 on those isolates sharing the same INMV pattern.
Instituto de Biotecnología
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Imperiale, Belen Rocio. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX); Argentina
Fil: Imperiale, Belen Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Magalí. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria (CEDIVE); Argentina
Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Santangelo, María De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarado Pinedo, Maria Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria (CEDIVE); Argentina
Fil: Travería, Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria (CEDIVE); Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Background: Paratuberculosis is an enteric disease caused by Mycobacterium avium sp. paratuberculosis (MAP) that affects mainly ruminant producing losses to the livestock industry. Many molecular epidemiological methods have been used to discriminate MAP isolates. Method: The aim of this study was to describe the genetic diversity of the Argentinean MAP isolates using a combination of two molecular systems, the mycobacterial interspersed repetitive unit–variable number tandem repeat (MIRU-VNTR) (“automated and “non-automated”) and the multi-locus short-sequence repeat (MLSSR) system. Results: Thirty-two isolates were identified as MAP of C type by IS900 polymerase chain reaction (PCA) and IS1311 PCA-restriction enzyme analysis. The main patterns found by both MIRU-VNTR systems were INMV1 (54.5%), INMV2 (24.2%) and INMV11 (9.1%). The INMV5, INMV8 and INMV16 were represented with one isolate each (3.0%). Only 4 MIRU-VNTR loci were polymorphic. Conclusion: Those isolates sharing the same INMV patterns were analyzed by MLSSR, being locus 2 the most polymorphic one showing isolates with 9, 10, 11, and more than 11 “G” repeats. Besides, the global discriminatory power among isolates could be increased using both techniques. Based on these results, a short version of the “automated” MIRU-VNTR could be used as a screening tool to group isolates genetically related and subsequently perform the SSR using locus 2 on those isolates sharing the same INMV pattern.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-14T10:45:47Z
2021-04-14T10:45:47Z
2021-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9081
https://www.ijmyco.org/article.asp?issn=2212-5531;year=2021;volume=10;issue=1;spage=51;epage=59;aulast=Moyano
2212-5531
https://doi.org/10.4103/ijmy.ijmy_229_20
url http://hdl.handle.net/20.500.12123/9081
https://www.ijmyco.org/article.asp?issn=2212-5531;year=2021;volume=10;issue=1;spage=51;epage=59;aulast=Moyano
https://doi.org/10.4103/ijmy.ijmy_229_20
identifier_str_mv 2212-5531
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wolters Kluwer Medknow
publisher.none.fl_str_mv Wolters Kluwer Medknow
dc.source.none.fl_str_mv International Journal of Mycobacteriology 10 (1) : 51-59 (Marzo 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341386305667072
score 12.623145

Publicaciones similares