Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis

Autores
Mon, María Laura; Viale, Mariana; Baschetti, Guido; Alvarado Pinedo, Fiorella; Gioffre, Andrea; Travería, Gabriel Eduardo; Willemsen, Peter; Bakker, Douwe; Romano, María Isabel
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to evaluate a wide panel of antigens of Mycobacterium avium subsp. paratuberculosis (MAP) to select candidates for the diagnosis of paratuberculosis (PTB). A total of 54 recombinant proteins were spotted onto nitrocellulose membranes and exposed to sera from animals with PTB (n=25), healthy animals (n=10), and animals experimentally infected with M. bovis (n=8). This initial screening allowed us to select seven antigens: MAP 2513, MAP 1693, MAP 2020, MAP 0038, MAP 1272, MAP 0209c, and MAP 0210c, which reacted with sera from animals with PTB and showed little cross-reactivity with sera from healthy animals and animals experimentally infected with M. bovis. The second step was to evaluate the antigen cocktail of these seven antigens by ELISA. For this evaluation, we used sera from animals with PTB (n=25), healthy animals (n=26), and animals experimentally infected with M. bovis (n=17). Using ELISA, the cocktail of the seven selected MAP antigens reacted with sera from 18 of the 25 animals with PTB and did not exhibit cross-reactivity with healthy animals and only low reactivity with animals with bovine tuberculosis. The combined application of these antigens could form part of a test which may help in the diagnosis of PTB.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Mycobacterium avium
Bacterias
Paratuberculosis
Antígenos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/3.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/29177

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network_name_str SEDICI (UNLP)
spelling Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosisMon, María LauraViale, MarianaBaschetti, GuidoAlvarado Pinedo, FiorellaGioffre, AndreaTravería, Gabriel EduardoWillemsen, PeterBakker, DouweRomano, María IsabelCiencias VeterinariasMycobacterium aviumBacteriasParatuberculosisAntígenosThe aim of this study was to evaluate a wide panel of antigens of Mycobacterium avium subsp. paratuberculosis (MAP) to select candidates for the diagnosis of paratuberculosis (PTB). A total of 54 recombinant proteins were spotted onto nitrocellulose membranes and exposed to sera from animals with PTB (n=25), healthy animals (n=10), and animals experimentally infected with M. bovis (n=8). This initial screening allowed us to select seven antigens: MAP 2513, MAP 1693, MAP 2020, MAP 0038, MAP 1272, MAP 0209c, and MAP 0210c, which reacted with sera from animals with PTB and showed little cross-reactivity with sera from healthy animals and animals experimentally infected with M. bovis. The second step was to evaluate the antigen cocktail of these seven antigens by ELISA. For this evaluation, we used sera from animals with PTB (n=25), healthy animals (n=26), and animals experimentally infected with M. bovis (n=17). Using ELISA, the cocktail of the seven selected MAP antigens reacted with sera from 18 of the 25 animals with PTB and did not exhibit cross-reactivity with healthy animals and only low reactivity with animals with bovine tuberculosis. The combined application of these antigens could form part of a test which may help in the diagnosis of PTB.Facultad de Ciencias Veterinarias2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/29177enginfo:eu-repo/semantics/altIdentifier/url/http://www.hindawi.com/journals/vmi/2012/860362/info:eu-repo/semantics/altIdentifier/issn/2042-0048info:eu-repo/semantics/altIdentifier/doi/10.1155/2012/860362info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/Creative Commons Attribution 3.0 Unported (CC BY 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:29:59Zoai:sedici.unlp.edu.ar:10915/29177Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:29:59.734SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis
title Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis
spellingShingle Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis
Mon, María Laura
Ciencias Veterinarias
Mycobacterium avium
Bacterias
Paratuberculosis
Antígenos
title_short Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis
title_full Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis
title_fullStr Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis
title_full_unstemmed Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis
title_sort Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis
dc.creator.none.fl_str_mv Mon, María Laura
Viale, Mariana
Baschetti, Guido
Alvarado Pinedo, Fiorella
Gioffre, Andrea
Travería, Gabriel Eduardo
Willemsen, Peter
Bakker, Douwe
Romano, María Isabel
author Mon, María Laura
author_facet Mon, María Laura
Viale, Mariana
Baschetti, Guido
Alvarado Pinedo, Fiorella
Gioffre, Andrea
Travería, Gabriel Eduardo
Willemsen, Peter
Bakker, Douwe
Romano, María Isabel
author_role author
author2 Viale, Mariana
Baschetti, Guido
Alvarado Pinedo, Fiorella
Gioffre, Andrea
Travería, Gabriel Eduardo
Willemsen, Peter
Bakker, Douwe
Romano, María Isabel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Mycobacterium avium
Bacterias
Paratuberculosis
Antígenos
topic Ciencias Veterinarias
Mycobacterium avium
Bacterias
Paratuberculosis
Antígenos
dc.description.none.fl_txt_mv The aim of this study was to evaluate a wide panel of antigens of Mycobacterium avium subsp. paratuberculosis (MAP) to select candidates for the diagnosis of paratuberculosis (PTB). A total of 54 recombinant proteins were spotted onto nitrocellulose membranes and exposed to sera from animals with PTB (n=25), healthy animals (n=10), and animals experimentally infected with M. bovis (n=8). This initial screening allowed us to select seven antigens: MAP 2513, MAP 1693, MAP 2020, MAP 0038, MAP 1272, MAP 0209c, and MAP 0210c, which reacted with sera from animals with PTB and showed little cross-reactivity with sera from healthy animals and animals experimentally infected with M. bovis. The second step was to evaluate the antigen cocktail of these seven antigens by ELISA. For this evaluation, we used sera from animals with PTB (n=25), healthy animals (n=26), and animals experimentally infected with M. bovis (n=17). Using ELISA, the cocktail of the seven selected MAP antigens reacted with sera from 18 of the 25 animals with PTB and did not exhibit cross-reactivity with healthy animals and only low reactivity with animals with bovine tuberculosis. The combined application of these antigens could form part of a test which may help in the diagnosis of PTB.
Facultad de Ciencias Veterinarias
description The aim of this study was to evaluate a wide panel of antigens of Mycobacterium avium subsp. paratuberculosis (MAP) to select candidates for the diagnosis of paratuberculosis (PTB). A total of 54 recombinant proteins were spotted onto nitrocellulose membranes and exposed to sera from animals with PTB (n=25), healthy animals (n=10), and animals experimentally infected with M. bovis (n=8). This initial screening allowed us to select seven antigens: MAP 2513, MAP 1693, MAP 2020, MAP 0038, MAP 1272, MAP 0209c, and MAP 0210c, which reacted with sera from animals with PTB and showed little cross-reactivity with sera from healthy animals and animals experimentally infected with M. bovis. The second step was to evaluate the antigen cocktail of these seven antigens by ELISA. For this evaluation, we used sera from animals with PTB (n=25), healthy animals (n=26), and animals experimentally infected with M. bovis (n=17). Using ELISA, the cocktail of the seven selected MAP antigens reacted with sera from 18 of the 25 animals with PTB and did not exhibit cross-reactivity with healthy animals and only low reactivity with animals with bovine tuberculosis. The combined application of these antigens could form part of a test which may help in the diagnosis of PTB.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/29177
url http://sedici.unlp.edu.ar/handle/10915/29177
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.hindawi.com/journals/vmi/2012/860362/
info:eu-repo/semantics/altIdentifier/issn/2042-0048
info:eu-repo/semantics/altIdentifier/doi/10.1155/2012/860362
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/
Creative Commons Attribution 3.0 Unported (CC BY 3.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/
Creative Commons Attribution 3.0 Unported (CC BY 3.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
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instname_str Universidad Nacional de La Plata
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institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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