Structural analysis of “annual ryegrass” (Lolium multiflorum Lam.) leaves under alkaline stress conditions

Autores
Gonzalez, Ana J.; Lagraña, Aldana A.; Acuña, Mariela Luciana; Pistorale, Susana M.; Larraburu, Ezequiel E.
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Halomorphic soils, widespread in many regions globally, pose significant agricultural challenges due to their high salinity and sodicity. These conditions create unfavorable environments for crop cultivation, especially forage species. In this context, the demand for sodic- and salt-tolerant plants is rising as aridity intensifies worldwide. Lolium multiflorum Lam. (annual ryegrass), a cold-season forage grass, is recognized for its high nutritional value, palatability, and potential uses in medicinal applications and bioethanol production. The anatomical adaptations of ryegrass under sodic stress remain insufficiently explored. This study aimed to investigate the effect of sodic soils on the leaf anatomy of L. multiflorum, focusing on micrometric parameters in the epidermis and cross-sections of leaves. The experiment was conducted with 35 genotypes of L. multiflorum grown in controlled conditions: one group in typical argiudol soils without restrictions and another in halomorphic soils with high pH (9.4) and electrical conductivity (3.74 dS/m). Leaf samples were collected and analyzed using light microscopy. Measurements included leaf thickness, mesophyll cell dimensions, epidermal thickness, stomatal size, and bulliform cell parameters. Results showed significant anatomical changes in plants grown in sodic soils. Notable findings included increased dimensions of bulliform and sheath cells linked to improved water conservation and stress adaptation. Conversely, the size of mesophyll and stomatal cells decreased, which aided in minimizing water loss while maintaining stomatal density and index, thereby ensuring sufficient gas exchange. These changes highlight the adaptive strategies of L. multiflorum to tolerate sodic conditions. This research contributes to understanding the morpho-anatomical adaptations that enhance plant resilience in challenging environments. Identifying anatomical traits associated with stress tolerance lays the groundwork for selecting and breeding genotypes suited to halomorphic soils. These insights are critical for developing sustainable agricultural practices and ensuring forage productivity in regions affected by soil salinity and sodicity. Ultimately, this study underscores the potential of L. multiflorum as a promising species for cultivation in degraded soils, offering a sustainable solution to the global challenge of increasing aridity and environmental stress.
EEA Pergamino
Fil: Gonzalez, Ana J. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
Fil: Lagraña, Aldana A. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
Fil: Acuña, Mariela Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Mejoramiento genético de especies forrajeras; Argentina
Fil: Acuña, Mariela Luciana. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y Ambientales; Argentina
Fil: Pistorale, Susana M. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
Fil: Pistorale, Susana M. Universidad Nacional del Noroeste de la provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y Ambientales; Argentina
Fil: Larraburu, Ezequiel E. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
Fil: Larraburu, Ezequiel E. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
South African Journal of Botany 191: 319-323. (April 2026)
Materia
Forrajes
Gramineas
Gramíneas Forrajeras
Anatomía de la Planta
Suelo Alcalino
Forage
Grasses
Lolium multiflorum
Poaceae
Feed Grasses
Plant Anatomy
Alkaline Soils
Osmotic Stress
Estrés Osmótico
Raigrás Anual
Salt Stress
Leaf Anatomy
Estrés Salino
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25642
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oai_identifier_str oai:localhost:20.500.12123/25642
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Structural analysis of “annual ryegrass” (Lolium multiflorum Lam.) leaves under alkaline stress conditionsGonzalez, Ana J.Lagraña, Aldana A.Acuña, Mariela LucianaPistorale, Susana M.Larraburu, Ezequiel E.ForrajesGramineasGramíneas ForrajerasAnatomía de la PlantaSuelo AlcalinoForageGrassesLolium multiflorumPoaceaeFeed GrassesPlant AnatomyAlkaline SoilsOsmotic StressEstrés OsmóticoRaigrás AnualSalt StressLeaf AnatomyEstrés SalinoHalomorphic soils, widespread in many regions globally, pose significant agricultural challenges due to their high salinity and sodicity. These conditions create unfavorable environments for crop cultivation, especially forage species. In this context, the demand for sodic- and salt-tolerant plants is rising as aridity intensifies worldwide. Lolium multiflorum Lam. (annual ryegrass), a cold-season forage grass, is recognized for its high nutritional value, palatability, and potential uses in medicinal applications and bioethanol production. The anatomical adaptations of ryegrass under sodic stress remain insufficiently explored. This study aimed to investigate the effect of sodic soils on the leaf anatomy of L. multiflorum, focusing on micrometric parameters in the epidermis and cross-sections of leaves. The experiment was conducted with 35 genotypes of L. multiflorum grown in controlled conditions: one group in typical argiudol soils without restrictions and another in halomorphic soils with high pH (9.4) and electrical conductivity (3.74 dS/m). Leaf samples were collected and analyzed using light microscopy. Measurements included leaf thickness, mesophyll cell dimensions, epidermal thickness, stomatal size, and bulliform cell parameters. Results showed significant anatomical changes in plants grown in sodic soils. Notable findings included increased dimensions of bulliform and sheath cells linked to improved water conservation and stress adaptation. Conversely, the size of mesophyll and stomatal cells decreased, which aided in minimizing water loss while maintaining stomatal density and index, thereby ensuring sufficient gas exchange. These changes highlight the adaptive strategies of L. multiflorum to tolerate sodic conditions. This research contributes to understanding the morpho-anatomical adaptations that enhance plant resilience in challenging environments. Identifying anatomical traits associated with stress tolerance lays the groundwork for selecting and breeding genotypes suited to halomorphic soils. These insights are critical for developing sustainable agricultural practices and ensuring forage productivity in regions affected by soil salinity and sodicity. Ultimately, this study underscores the potential of L. multiflorum as a promising species for cultivation in degraded soils, offering a sustainable solution to the global challenge of increasing aridity and environmental stress.EEA PergaminoFil: Gonzalez, Ana J. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Cultivo de Tejidos Vegetales; ArgentinaFil: Lagraña, Aldana A. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Cultivo de Tejidos Vegetales; ArgentinaFil: Acuña, Mariela Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Mejoramiento genético de especies forrajeras; ArgentinaFil: Acuña, Mariela Luciana. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y Ambientales; ArgentinaFil: Pistorale, Susana M. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Cultivo de Tejidos Vegetales; ArgentinaFil: Pistorale, Susana M. Universidad Nacional del Noroeste de la provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y Ambientales; ArgentinaFil: Larraburu, Ezequiel E. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Cultivo de Tejidos Vegetales; ArgentinaFil: Larraburu, Ezequiel E. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2026-03-31T11:56:59Z2026-03-31T11:56:59Z2026-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25642https://www.sciencedirect.com/science/article/abs/pii/S02546299260008030254-62991727-9321https://doi.org/10.1016/j.sajb.2026.02.021South African Journal of Botany 191: 319-323. (April 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-05-07T11:53:05Zoai:localhost:20.500.12123/25642instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-05-07 11:53:06.722INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Structural analysis of “annual ryegrass” (Lolium multiflorum Lam.) leaves under alkaline stress conditions
title Structural analysis of “annual ryegrass” (Lolium multiflorum Lam.) leaves under alkaline stress conditions
spellingShingle Structural analysis of “annual ryegrass” (Lolium multiflorum Lam.) leaves under alkaline stress conditions
Gonzalez, Ana J.
Forrajes
Gramineas
Gramíneas Forrajeras
Anatomía de la Planta
Suelo Alcalino
Forage
Grasses
Lolium multiflorum
Poaceae
Feed Grasses
Plant Anatomy
Alkaline Soils
Osmotic Stress
Estrés Osmótico
Raigrás Anual
Salt Stress
Leaf Anatomy
Estrés Salino
title_short Structural analysis of “annual ryegrass” (Lolium multiflorum Lam.) leaves under alkaline stress conditions
title_full Structural analysis of “annual ryegrass” (Lolium multiflorum Lam.) leaves under alkaline stress conditions
title_fullStr Structural analysis of “annual ryegrass” (Lolium multiflorum Lam.) leaves under alkaline stress conditions
title_full_unstemmed Structural analysis of “annual ryegrass” (Lolium multiflorum Lam.) leaves under alkaline stress conditions
title_sort Structural analysis of “annual ryegrass” (Lolium multiflorum Lam.) leaves under alkaline stress conditions
dc.creator.none.fl_str_mv Gonzalez, Ana J.
Lagraña, Aldana A.
Acuña, Mariela Luciana
Pistorale, Susana M.
Larraburu, Ezequiel E.
author Gonzalez, Ana J.
author_facet Gonzalez, Ana J.
Lagraña, Aldana A.
Acuña, Mariela Luciana
Pistorale, Susana M.
Larraburu, Ezequiel E.
author_role author
author2 Lagraña, Aldana A.
Acuña, Mariela Luciana
Pistorale, Susana M.
Larraburu, Ezequiel E.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Forrajes
Gramineas
Gramíneas Forrajeras
Anatomía de la Planta
Suelo Alcalino
Forage
Grasses
Lolium multiflorum
Poaceae
Feed Grasses
Plant Anatomy
Alkaline Soils
Osmotic Stress
Estrés Osmótico
Raigrás Anual
Salt Stress
Leaf Anatomy
Estrés Salino
topic Forrajes
Gramineas
Gramíneas Forrajeras
Anatomía de la Planta
Suelo Alcalino
Forage
Grasses
Lolium multiflorum
Poaceae
Feed Grasses
Plant Anatomy
Alkaline Soils
Osmotic Stress
Estrés Osmótico
Raigrás Anual
Salt Stress
Leaf Anatomy
Estrés Salino
dc.description.none.fl_txt_mv Halomorphic soils, widespread in many regions globally, pose significant agricultural challenges due to their high salinity and sodicity. These conditions create unfavorable environments for crop cultivation, especially forage species. In this context, the demand for sodic- and salt-tolerant plants is rising as aridity intensifies worldwide. Lolium multiflorum Lam. (annual ryegrass), a cold-season forage grass, is recognized for its high nutritional value, palatability, and potential uses in medicinal applications and bioethanol production. The anatomical adaptations of ryegrass under sodic stress remain insufficiently explored. This study aimed to investigate the effect of sodic soils on the leaf anatomy of L. multiflorum, focusing on micrometric parameters in the epidermis and cross-sections of leaves. The experiment was conducted with 35 genotypes of L. multiflorum grown in controlled conditions: one group in typical argiudol soils without restrictions and another in halomorphic soils with high pH (9.4) and electrical conductivity (3.74 dS/m). Leaf samples were collected and analyzed using light microscopy. Measurements included leaf thickness, mesophyll cell dimensions, epidermal thickness, stomatal size, and bulliform cell parameters. Results showed significant anatomical changes in plants grown in sodic soils. Notable findings included increased dimensions of bulliform and sheath cells linked to improved water conservation and stress adaptation. Conversely, the size of mesophyll and stomatal cells decreased, which aided in minimizing water loss while maintaining stomatal density and index, thereby ensuring sufficient gas exchange. These changes highlight the adaptive strategies of L. multiflorum to tolerate sodic conditions. This research contributes to understanding the morpho-anatomical adaptations that enhance plant resilience in challenging environments. Identifying anatomical traits associated with stress tolerance lays the groundwork for selecting and breeding genotypes suited to halomorphic soils. These insights are critical for developing sustainable agricultural practices and ensuring forage productivity in regions affected by soil salinity and sodicity. Ultimately, this study underscores the potential of L. multiflorum as a promising species for cultivation in degraded soils, offering a sustainable solution to the global challenge of increasing aridity and environmental stress.
EEA Pergamino
Fil: Gonzalez, Ana J. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
Fil: Lagraña, Aldana A. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
Fil: Acuña, Mariela Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Mejoramiento genético de especies forrajeras; Argentina
Fil: Acuña, Mariela Luciana. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y Ambientales; Argentina
Fil: Pistorale, Susana M. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
Fil: Pistorale, Susana M. Universidad Nacional del Noroeste de la provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y Ambientales; Argentina
Fil: Larraburu, Ezequiel E. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Cultivo de Tejidos Vegetales; Argentina
Fil: Larraburu, Ezequiel E. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Halomorphic soils, widespread in many regions globally, pose significant agricultural challenges due to their high salinity and sodicity. These conditions create unfavorable environments for crop cultivation, especially forage species. In this context, the demand for sodic- and salt-tolerant plants is rising as aridity intensifies worldwide. Lolium multiflorum Lam. (annual ryegrass), a cold-season forage grass, is recognized for its high nutritional value, palatability, and potential uses in medicinal applications and bioethanol production. The anatomical adaptations of ryegrass under sodic stress remain insufficiently explored. This study aimed to investigate the effect of sodic soils on the leaf anatomy of L. multiflorum, focusing on micrometric parameters in the epidermis and cross-sections of leaves. The experiment was conducted with 35 genotypes of L. multiflorum grown in controlled conditions: one group in typical argiudol soils without restrictions and another in halomorphic soils with high pH (9.4) and electrical conductivity (3.74 dS/m). Leaf samples were collected and analyzed using light microscopy. Measurements included leaf thickness, mesophyll cell dimensions, epidermal thickness, stomatal size, and bulliform cell parameters. Results showed significant anatomical changes in plants grown in sodic soils. Notable findings included increased dimensions of bulliform and sheath cells linked to improved water conservation and stress adaptation. Conversely, the size of mesophyll and stomatal cells decreased, which aided in minimizing water loss while maintaining stomatal density and index, thereby ensuring sufficient gas exchange. These changes highlight the adaptive strategies of L. multiflorum to tolerate sodic conditions. This research contributes to understanding the morpho-anatomical adaptations that enhance plant resilience in challenging environments. Identifying anatomical traits associated with stress tolerance lays the groundwork for selecting and breeding genotypes suited to halomorphic soils. These insights are critical for developing sustainable agricultural practices and ensuring forage productivity in regions affected by soil salinity and sodicity. Ultimately, this study underscores the potential of L. multiflorum as a promising species for cultivation in degraded soils, offering a sustainable solution to the global challenge of increasing aridity and environmental stress.
publishDate 2026
dc.date.none.fl_str_mv 2026-03-31T11:56:59Z
2026-03-31T11:56:59Z
2026-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25642
https://www.sciencedirect.com/science/article/abs/pii/S0254629926000803
0254-6299
1727-9321
https://doi.org/10.1016/j.sajb.2026.02.021
url http://hdl.handle.net/20.500.12123/25642
https://www.sciencedirect.com/science/article/abs/pii/S0254629926000803
https://doi.org/10.1016/j.sajb.2026.02.021
identifier_str_mv 0254-6299
1727-9321
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv South African Journal of Botany 191: 319-323. (April 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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