Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens

Autores
Agudelo Romero, Patricia; Fortes, Ana Margarida; Suárez, Trinidad; Lascano, Hernan Ramiro; Saavedra Borelli, Laura Lucía
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Phosphatidylinositol 3-phosphate (PtdIns3P) is a signaling phospholipid, which regulates several aspects of plant growth and development, as well as responses to biotic and abiotic stresses. The mechanistic insights underlying PtdIns3P mode of action, specifcally through efector proteins have been partially explored in plants, with main focus on Arabidopsis thaliana. In this study, we searched for genes coding for PtdIns3P-binding proteins such as FYVE and PHOX domaincontaining sequences from diferent photosynthetic organisms to gather evolutionary insights on these phosphoinositide binding domains, followed by an in silico characterization of the FYVE and PHOX gene families in the moss Physcomitrella patens. Phylogenetic analysis showed that PpFYVE proteins can be grouped in 7 subclasses, with an additional subclass whose FYVE domain was lost during evolution to higher plants. On the other hand, PpPHOX proteins are classifed into 5 subclasses. Expression analyses based on RNAseq data together with the analysis of cis-acting regulatory elements and transcription factor (TF) binding sites in promoter regions suggest the importance of these proteins in regulating stress responses but mainly developmental processes in P. patens. The results provide valuable information and robust candidate genes for future functional analysis aiming to further explore the role of this signaling pathway mainly during growth and development of tip growing cells and during the transition from 2 to 3D growth. These studies would identify ancestral regulatory players undertaken during plant evolution.
Fil: Agudelo Romero, Patricia. University Of Western Australia. Faculty Of Science. Centre Of Excellence In Plant Energy Biology.; Australia
Fil: Fortes, Ana Margarida. Universidade de Lisboa; Portugal
Fil: Suárez, Trinidad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; Argentina
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Estudios Agropecuarios.; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; Argentina
Fil: Saavedra Borelli, Laura Lucía. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Materia
BRYOPHYTES
PHOSPHATIDYLINOSITOL 3-PHOSPHATE
PHOSPHATIDYLINOSITOL 3,5 -BIPHOSPHATE
PHOSPHOINOSITIDES
VESICLE TRAFCKING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/112612
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patensAgudelo Romero, PatriciaFortes, Ana MargaridaSuárez, TrinidadLascano, Hernan RamiroSaavedra Borelli, Laura LucíaBRYOPHYTESPHOSPHATIDYLINOSITOL 3-PHOSPHATEPHOSPHATIDYLINOSITOL 3,5 -BIPHOSPHATEPHOSPHOINOSITIDESVESICLE TRAFCKINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Phosphatidylinositol 3-phosphate (PtdIns3P) is a signaling phospholipid, which regulates several aspects of plant growth and development, as well as responses to biotic and abiotic stresses. The mechanistic insights underlying PtdIns3P mode of action, specifcally through efector proteins have been partially explored in plants, with main focus on Arabidopsis thaliana. In this study, we searched for genes coding for PtdIns3P-binding proteins such as FYVE and PHOX domaincontaining sequences from diferent photosynthetic organisms to gather evolutionary insights on these phosphoinositide binding domains, followed by an in silico characterization of the FYVE and PHOX gene families in the moss Physcomitrella patens. Phylogenetic analysis showed that PpFYVE proteins can be grouped in 7 subclasses, with an additional subclass whose FYVE domain was lost during evolution to higher plants. On the other hand, PpPHOX proteins are classifed into 5 subclasses. Expression analyses based on RNAseq data together with the analysis of cis-acting regulatory elements and transcription factor (TF) binding sites in promoter regions suggest the importance of these proteins in regulating stress responses but mainly developmental processes in P. patens. The results provide valuable information and robust candidate genes for future functional analysis aiming to further explore the role of this signaling pathway mainly during growth and development of tip growing cells and during the transition from 2 to 3D growth. These studies would identify ancestral regulatory players undertaken during plant evolution.Fil: Agudelo Romero, Patricia. University Of Western Australia. Faculty Of Science. Centre Of Excellence In Plant Energy Biology.; AustraliaFil: Fortes, Ana Margarida. Universidade de Lisboa; PortugalFil: Suárez, Trinidad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; ArgentinaFil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Estudios Agropecuarios.; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; ArgentinaFil: Saavedra Borelli, Laura Lucía. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaSpringer2020-02-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/112612Agudelo Romero, Patricia; Fortes, Ana Margarida; Suárez, Trinidad; Lascano, Hernan Ramiro; Saavedra Borelli, Laura Lucía; Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens; Springer; Planta; 251; 62; 10-2-2020; 1-200032-09351432-2048CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00425-020-03354-winfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00425-020-03354-winfo:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/765594v1info:eu-repo/semantics/altIdentifier/url/https://repositorio.inta.gob.ar/xmlui/handle/20.500.12123/7067info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:35Zoai:ri.conicet.gov.ar:11336/112612instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:35.357CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
title Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
spellingShingle Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
Agudelo Romero, Patricia
BRYOPHYTES
PHOSPHATIDYLINOSITOL 3-PHOSPHATE
PHOSPHATIDYLINOSITOL 3,5 -BIPHOSPHATE
PHOSPHOINOSITIDES
VESICLE TRAFCKING
title_short Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
title_full Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
title_fullStr Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
title_full_unstemmed Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
title_sort Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
dc.creator.none.fl_str_mv Agudelo Romero, Patricia
Fortes, Ana Margarida
Suárez, Trinidad
Lascano, Hernan Ramiro
Saavedra Borelli, Laura Lucía
author Agudelo Romero, Patricia
author_facet Agudelo Romero, Patricia
Fortes, Ana Margarida
Suárez, Trinidad
Lascano, Hernan Ramiro
Saavedra Borelli, Laura Lucía
author_role author
author2 Fortes, Ana Margarida
Suárez, Trinidad
Lascano, Hernan Ramiro
Saavedra Borelli, Laura Lucía
author2_role author
author
author
author
dc.subject.none.fl_str_mv BRYOPHYTES
PHOSPHATIDYLINOSITOL 3-PHOSPHATE
PHOSPHATIDYLINOSITOL 3,5 -BIPHOSPHATE
PHOSPHOINOSITIDES
VESICLE TRAFCKING
topic BRYOPHYTES
PHOSPHATIDYLINOSITOL 3-PHOSPHATE
PHOSPHATIDYLINOSITOL 3,5 -BIPHOSPHATE
PHOSPHOINOSITIDES
VESICLE TRAFCKING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Phosphatidylinositol 3-phosphate (PtdIns3P) is a signaling phospholipid, which regulates several aspects of plant growth and development, as well as responses to biotic and abiotic stresses. The mechanistic insights underlying PtdIns3P mode of action, specifcally through efector proteins have been partially explored in plants, with main focus on Arabidopsis thaliana. In this study, we searched for genes coding for PtdIns3P-binding proteins such as FYVE and PHOX domaincontaining sequences from diferent photosynthetic organisms to gather evolutionary insights on these phosphoinositide binding domains, followed by an in silico characterization of the FYVE and PHOX gene families in the moss Physcomitrella patens. Phylogenetic analysis showed that PpFYVE proteins can be grouped in 7 subclasses, with an additional subclass whose FYVE domain was lost during evolution to higher plants. On the other hand, PpPHOX proteins are classifed into 5 subclasses. Expression analyses based on RNAseq data together with the analysis of cis-acting regulatory elements and transcription factor (TF) binding sites in promoter regions suggest the importance of these proteins in regulating stress responses but mainly developmental processes in P. patens. The results provide valuable information and robust candidate genes for future functional analysis aiming to further explore the role of this signaling pathway mainly during growth and development of tip growing cells and during the transition from 2 to 3D growth. These studies would identify ancestral regulatory players undertaken during plant evolution.
Fil: Agudelo Romero, Patricia. University Of Western Australia. Faculty Of Science. Centre Of Excellence In Plant Energy Biology.; Australia
Fil: Fortes, Ana Margarida. Universidade de Lisboa; Portugal
Fil: Suárez, Trinidad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; Argentina
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Estudios Agropecuarios.; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; Argentina
Fil: Saavedra Borelli, Laura Lucía. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Fisiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
description Phosphatidylinositol 3-phosphate (PtdIns3P) is a signaling phospholipid, which regulates several aspects of plant growth and development, as well as responses to biotic and abiotic stresses. The mechanistic insights underlying PtdIns3P mode of action, specifcally through efector proteins have been partially explored in plants, with main focus on Arabidopsis thaliana. In this study, we searched for genes coding for PtdIns3P-binding proteins such as FYVE and PHOX domaincontaining sequences from diferent photosynthetic organisms to gather evolutionary insights on these phosphoinositide binding domains, followed by an in silico characterization of the FYVE and PHOX gene families in the moss Physcomitrella patens. Phylogenetic analysis showed that PpFYVE proteins can be grouped in 7 subclasses, with an additional subclass whose FYVE domain was lost during evolution to higher plants. On the other hand, PpPHOX proteins are classifed into 5 subclasses. Expression analyses based on RNAseq data together with the analysis of cis-acting regulatory elements and transcription factor (TF) binding sites in promoter regions suggest the importance of these proteins in regulating stress responses but mainly developmental processes in P. patens. The results provide valuable information and robust candidate genes for future functional analysis aiming to further explore the role of this signaling pathway mainly during growth and development of tip growing cells and during the transition from 2 to 3D growth. These studies would identify ancestral regulatory players undertaken during plant evolution.
publishDate 2020
dc.date.none.fl_str_mv 2020-02-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/112612
Agudelo Romero, Patricia; Fortes, Ana Margarida; Suárez, Trinidad; Lascano, Hernan Ramiro; Saavedra Borelli, Laura Lucía; Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens; Springer; Planta; 251; 62; 10-2-2020; 1-20
0032-0935
1432-2048
CONICET Digital
CONICET
url http://hdl.handle.net/11336/112612
identifier_str_mv Agudelo Romero, Patricia; Fortes, Ana Margarida; Suárez, Trinidad; Lascano, Hernan Ramiro; Saavedra Borelli, Laura Lucía; Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens; Springer; Planta; 251; 62; 10-2-2020; 1-20
0032-0935
1432-2048
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00425-020-03354-w
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00425-020-03354-w
info:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/765594v1
info:eu-repo/semantics/altIdentifier/url/https://repositorio.inta.gob.ar/xmlui/handle/20.500.12123/7067
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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