Early-stage findings in an experimental calf model infected with Argentinean isolates of Mycobacterium avium subsp. Paratuberculosis

Autores
Ingratta, Giselle Gabriela; Stempler, Ana; Fernández, Bárbara; Colavecchia, Silvia Beatriz; Jolly, Ana; Minatel, Leonardo; Paolicchi, Fernando; Mundo, Silvia Leonor
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mycobacterium avium subsp. paratuberculosis (MAP) is an important pathogen that causes granulomatous enteritis known as Johne’s disease or paratuberculosis (PTB). In this study an experimental model of calves infected with Argentinean isolates of MAP for 180 days was used to provide more data of the early PTB stages. Calves were challenged by oral route with MAP strain IS900-RFLPA (MA; n = 3), MAP strain IS900-RFLPC (MC; n = 2) or mock infected (MI; n = 2), and response to infection was evaluated through peripheral cytokine expression, MAP tissue distribution and histopathological early-stage findings. Specific and varied levels of IFN-γ were only detected at 80 days post-infection in infected calves. These data indicate that specific IFN-γ is not a useful indicator for early detection of MAP infection in our calf model. At 110 days post-infection, TNF-α expression was higher than IL-10 in 4 of the 5 infected animals and a significant decrease of TNF-α expression was detected in infected vs. non-infected calves. All calves challenged were identified as infected by mesenteric lymph node tissue culture and real time IS900 PCR. In addition, for lymph nodes samples, the agreement between these techniques was almost perfect (κ = 0.86). Colonization of tissues and levels of tissue infection varied between individuals. Evidence of early MAP dissemination to extraintestinal tissues such as the liver was detected by culture in one animal (MAP strain IS900-RFLPA). In both groups microgranulomatous lesions were observed predominantly in the lymph nodes, with giant cells present only in the MA group. In summary, the findings described herein may indicate that local MAP strains induced specific immune responses with particularities that could suggest differences in their biological behavior. Further studies should be carried out in order to obtain an in-depth understanding of the influence of MAP strains in host-pathogen interactions and the outcome of disease.
EEA Balcarce
Fil: Ingratta, Giselle Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Stempler, Ana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Fernández, Bárbara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Fernández, Bárbara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Colavecchia, Silvia Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Jolly, Ana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Minatel, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Paolicchi, Fernando Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Paolicchi, Fernando Alberto. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Mundo, Silvia Leonor. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Mundo, Silvia Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fuente
Veterinary Immunology and Immunopathology 259 : 110595 (May 2023)
Materia
Ternero
Infección Experimental
Histología Animal
Mapa
Calves
Experimental Infection
Animal Histology
Maps
Mycobacterium Avium Subsp Paratuberculosis
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/16013
Acceder
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oai_identifier_str oai:localhost:20.500.12123/16013
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Early-stage findings in an experimental calf model infected with Argentinean isolates of Mycobacterium avium subsp. ParatuberculosisIngratta, Giselle GabrielaStempler, AnaFernández, BárbaraColavecchia, Silvia BeatrizJolly, AnaMinatel, LeonardoPaolicchi, FernandoMundo, Silvia LeonorTerneroInfección ExperimentalHistología AnimalMapaCalvesExperimental InfectionAnimal HistologyMapsMycobacterium Avium Subsp ParatuberculosisMycobacterium avium subsp. paratuberculosis (MAP) is an important pathogen that causes granulomatous enteritis known as Johne’s disease or paratuberculosis (PTB). In this study an experimental model of calves infected with Argentinean isolates of MAP for 180 days was used to provide more data of the early PTB stages. Calves were challenged by oral route with MAP strain IS900-RFLPA (MA; n = 3), MAP strain IS900-RFLPC (MC; n = 2) or mock infected (MI; n = 2), and response to infection was evaluated through peripheral cytokine expression, MAP tissue distribution and histopathological early-stage findings. Specific and varied levels of IFN-γ were only detected at 80 days post-infection in infected calves. These data indicate that specific IFN-γ is not a useful indicator for early detection of MAP infection in our calf model. At 110 days post-infection, TNF-α expression was higher than IL-10 in 4 of the 5 infected animals and a significant decrease of TNF-α expression was detected in infected vs. non-infected calves. All calves challenged were identified as infected by mesenteric lymph node tissue culture and real time IS900 PCR. In addition, for lymph nodes samples, the agreement between these techniques was almost perfect (κ = 0.86). Colonization of tissues and levels of tissue infection varied between individuals. Evidence of early MAP dissemination to extraintestinal tissues such as the liver was detected by culture in one animal (MAP strain IS900-RFLPA). In both groups microgranulomatous lesions were observed predominantly in the lymph nodes, with giant cells present only in the MA group. In summary, the findings described herein may indicate that local MAP strains induced specific immune responses with particularities that could suggest differences in their biological behavior. Further studies should be carried out in order to obtain an in-depth understanding of the influence of MAP strains in host-pathogen interactions and the outcome of disease.EEA BalcarceFil: Ingratta, Giselle Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.Fil: Stempler, Ana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.Fil: Fernández, Bárbara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.Fil: Fernández, Bárbara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Colavecchia, Silvia Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.Fil: Jolly, Ana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.Fil: Minatel, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.Fil: Paolicchi, Fernando Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Paolicchi, Fernando Alberto. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Mundo, Silvia Leonor. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.Fil: Mundo, Silvia Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Elsevier2023-11-27T10:44:49Z2023-11-27T10:44:49Z2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/16013https://www.sciencedirect.com/science/article/pii/S01652427230004911873-2534 (Online)0165-2427 (Print)https://doi.org/10.1016/j.vetimm.2023.110595Veterinary Immunology and Immunopathology 259 : 110595 (May 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E5-I103-001, Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud públicainfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:13Zoai:localhost:20.500.12123/16013instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:14.008INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Early-stage findings in an experimental calf model infected with Argentinean isolates of Mycobacterium avium subsp. Paratuberculosis
title Early-stage findings in an experimental calf model infected with Argentinean isolates of Mycobacterium avium subsp. Paratuberculosis
spellingShingle Early-stage findings in an experimental calf model infected with Argentinean isolates of Mycobacterium avium subsp. Paratuberculosis
Ingratta, Giselle Gabriela
Ternero
Infección Experimental
Histología Animal
Mapa
Calves
Experimental Infection
Animal Histology
Maps
Mycobacterium Avium Subsp Paratuberculosis
title_short Early-stage findings in an experimental calf model infected with Argentinean isolates of Mycobacterium avium subsp. Paratuberculosis
title_full Early-stage findings in an experimental calf model infected with Argentinean isolates of Mycobacterium avium subsp. Paratuberculosis
title_fullStr Early-stage findings in an experimental calf model infected with Argentinean isolates of Mycobacterium avium subsp. Paratuberculosis
title_full_unstemmed Early-stage findings in an experimental calf model infected with Argentinean isolates of Mycobacterium avium subsp. Paratuberculosis
title_sort Early-stage findings in an experimental calf model infected with Argentinean isolates of Mycobacterium avium subsp. Paratuberculosis
dc.creator.none.fl_str_mv Ingratta, Giselle Gabriela
Stempler, Ana
Fernández, Bárbara
Colavecchia, Silvia Beatriz
Jolly, Ana
Minatel, Leonardo
Paolicchi, Fernando
Mundo, Silvia Leonor
author Ingratta, Giselle Gabriela
author_facet Ingratta, Giselle Gabriela
Stempler, Ana
Fernández, Bárbara
Colavecchia, Silvia Beatriz
Jolly, Ana
Minatel, Leonardo
Paolicchi, Fernando
Mundo, Silvia Leonor
author_role author
author2 Stempler, Ana
Fernández, Bárbara
Colavecchia, Silvia Beatriz
Jolly, Ana
Minatel, Leonardo
Paolicchi, Fernando
Mundo, Silvia Leonor
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ternero
Infección Experimental
Histología Animal
Mapa
Calves
Experimental Infection
Animal Histology
Maps
Mycobacterium Avium Subsp Paratuberculosis
topic Ternero
Infección Experimental
Histología Animal
Mapa
Calves
Experimental Infection
Animal Histology
Maps
Mycobacterium Avium Subsp Paratuberculosis
dc.description.none.fl_txt_mv Mycobacterium avium subsp. paratuberculosis (MAP) is an important pathogen that causes granulomatous enteritis known as Johne’s disease or paratuberculosis (PTB). In this study an experimental model of calves infected with Argentinean isolates of MAP for 180 days was used to provide more data of the early PTB stages. Calves were challenged by oral route with MAP strain IS900-RFLPA (MA; n = 3), MAP strain IS900-RFLPC (MC; n = 2) or mock infected (MI; n = 2), and response to infection was evaluated through peripheral cytokine expression, MAP tissue distribution and histopathological early-stage findings. Specific and varied levels of IFN-γ were only detected at 80 days post-infection in infected calves. These data indicate that specific IFN-γ is not a useful indicator for early detection of MAP infection in our calf model. At 110 days post-infection, TNF-α expression was higher than IL-10 in 4 of the 5 infected animals and a significant decrease of TNF-α expression was detected in infected vs. non-infected calves. All calves challenged were identified as infected by mesenteric lymph node tissue culture and real time IS900 PCR. In addition, for lymph nodes samples, the agreement between these techniques was almost perfect (κ = 0.86). Colonization of tissues and levels of tissue infection varied between individuals. Evidence of early MAP dissemination to extraintestinal tissues such as the liver was detected by culture in one animal (MAP strain IS900-RFLPA). In both groups microgranulomatous lesions were observed predominantly in the lymph nodes, with giant cells present only in the MA group. In summary, the findings described herein may indicate that local MAP strains induced specific immune responses with particularities that could suggest differences in their biological behavior. Further studies should be carried out in order to obtain an in-depth understanding of the influence of MAP strains in host-pathogen interactions and the outcome of disease.
EEA Balcarce
Fil: Ingratta, Giselle Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Stempler, Ana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Fernández, Bárbara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Fernández, Bárbara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Colavecchia, Silvia Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Jolly, Ana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Minatel, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Paolicchi, Fernando Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Paolicchi, Fernando Alberto. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Mundo, Silvia Leonor. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Mundo, Silvia Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
description Mycobacterium avium subsp. paratuberculosis (MAP) is an important pathogen that causes granulomatous enteritis known as Johne’s disease or paratuberculosis (PTB). In this study an experimental model of calves infected with Argentinean isolates of MAP for 180 days was used to provide more data of the early PTB stages. Calves were challenged by oral route with MAP strain IS900-RFLPA (MA; n = 3), MAP strain IS900-RFLPC (MC; n = 2) or mock infected (MI; n = 2), and response to infection was evaluated through peripheral cytokine expression, MAP tissue distribution and histopathological early-stage findings. Specific and varied levels of IFN-γ were only detected at 80 days post-infection in infected calves. These data indicate that specific IFN-γ is not a useful indicator for early detection of MAP infection in our calf model. At 110 days post-infection, TNF-α expression was higher than IL-10 in 4 of the 5 infected animals and a significant decrease of TNF-α expression was detected in infected vs. non-infected calves. All calves challenged were identified as infected by mesenteric lymph node tissue culture and real time IS900 PCR. In addition, for lymph nodes samples, the agreement between these techniques was almost perfect (κ = 0.86). Colonization of tissues and levels of tissue infection varied between individuals. Evidence of early MAP dissemination to extraintestinal tissues such as the liver was detected by culture in one animal (MAP strain IS900-RFLPA). In both groups microgranulomatous lesions were observed predominantly in the lymph nodes, with giant cells present only in the MA group. In summary, the findings described herein may indicate that local MAP strains induced specific immune responses with particularities that could suggest differences in their biological behavior. Further studies should be carried out in order to obtain an in-depth understanding of the influence of MAP strains in host-pathogen interactions and the outcome of disease.
publishDate 2023
dc.date.none.fl_str_mv 2023-11-27T10:44:49Z
2023-11-27T10:44:49Z
2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/16013
https://www.sciencedirect.com/science/article/pii/S0165242723000491
1873-2534 (Online)
0165-2427 (Print)
https://doi.org/10.1016/j.vetimm.2023.110595
url http://hdl.handle.net/20.500.12123/16013
https://www.sciencedirect.com/science/article/pii/S0165242723000491
https://doi.org/10.1016/j.vetimm.2023.110595
identifier_str_mv 1873-2534 (Online)
0165-2427 (Print)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E5-I103-001, Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud pública
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Immunology and Immunopathology 259 : 110595 (May 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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