Immunological findings associated with Argentinean strains of Mycobacterium avium subsp. paratuberculosis in bovine models

Autores
Colavecchia, Silvia Beatriz; Fernández, Bárbara; Jolly, Ana; Minatel, Leonardo; Hajos, Silvia Elvira; Paolicchi, Fernando; Mundo, Silvia Leonor
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of ruminant paratuberculosis. The aim of this study was to evaluate the biological behavior of different Argentinean strains of MAP in two bovine infection models: macrophage (in vitro) and calf (in vivo) through the evaluation of early immune responses at the peripheral and local levels. Two MAP strains (A and C) were selected taking into account the different patterns of TNF-α and IL-10 secretion displayed by infected bovine macrophages in vitro. Two groups of calves were infected with 250 mg of total wet weight live MAP: strain A infected group (MA, n = 3), strain C infected group (MC, n = 2). Another group of animals was mock-infected (MI, n = 3). Infection was confirmed by MAP culture of feces and microscopic observation of granulomatous lesions in the gut tissue. All infected calves showed positive results in the DTH skin test. A significant increase in peripheral CD4CD25+ cells in MC group on day 150 was detected. The specific cellular immune response developed allowed the identification of the infection as early as 30 days in the MA group. However, the percentage of CD8CD25+ cells was significantly increased on day 120 in MC group. Significant differences between groups in proliferation and cellular responses were also detected in ileocecal lymph node samples. In summary, the strains of MAP employed herein induced differential immune responses in peripheral cells, in the proliferative responses and in cell functionality at the local level. Our findings support the hypotheses that the in vitro behavior displayed by macrophages could be a tool to identify differences among MAP strains infecting bovines and that the host-pathogen interactions occurring upon infection are dependent on the strain of MAP involved.
EEA Balcarce
Fil: Colavecchia, Silvia Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina
Fil: Fernández, Bárbara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina
Fil: Jolly, Ana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina
Fil: Minatel, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Patología; Argentina
Fil: Hajos, Silvia Elvira. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Inmunología; Argentina
Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Bacteriología. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Mundo, Silvia Leonor. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina
Fuente
Veterinary Immunology and Immunopathology 176 : 28-33 (August 2016)
Materia
Mycobacterium avium subsp. paratuberculosis
Enfermedades de los Animales
Inmunología
Respuesta Inmunológica
Ternero
Animal Diseases
Immunology
Immune Response
Calves
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4980
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4980
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Immunological findings associated with Argentinean strains of Mycobacterium avium subsp. paratuberculosis in bovine modelsColavecchia, Silvia BeatrizFernández, BárbaraJolly, AnaMinatel, LeonardoHajos, Silvia ElviraPaolicchi, FernandoMundo, Silvia LeonorMycobacterium avium subsp. paratuberculosisEnfermedades de los AnimalesInmunologíaRespuesta InmunológicaTerneroAnimal DiseasesImmunologyImmune ResponseCalvesMycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of ruminant paratuberculosis. The aim of this study was to evaluate the biological behavior of different Argentinean strains of MAP in two bovine infection models: macrophage (in vitro) and calf (in vivo) through the evaluation of early immune responses at the peripheral and local levels. Two MAP strains (A and C) were selected taking into account the different patterns of TNF-α and IL-10 secretion displayed by infected bovine macrophages in vitro. Two groups of calves were infected with 250 mg of total wet weight live MAP: strain A infected group (MA, n = 3), strain C infected group (MC, n = 2). Another group of animals was mock-infected (MI, n = 3). Infection was confirmed by MAP culture of feces and microscopic observation of granulomatous lesions in the gut tissue. All infected calves showed positive results in the DTH skin test. A significant increase in peripheral CD4CD25+ cells in MC group on day 150 was detected. The specific cellular immune response developed allowed the identification of the infection as early as 30 days in the MA group. However, the percentage of CD8CD25+ cells was significantly increased on day 120 in MC group. Significant differences between groups in proliferation and cellular responses were also detected in ileocecal lymph node samples. In summary, the strains of MAP employed herein induced differential immune responses in peripheral cells, in the proliferative responses and in cell functionality at the local level. Our findings support the hypotheses that the in vitro behavior displayed by macrophages could be a tool to identify differences among MAP strains infecting bovines and that the host-pathogen interactions occurring upon infection are dependent on the strain of MAP involved.EEA BalcarceFil: Colavecchia, Silvia Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; ArgentinaFil: Fernández, Bárbara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; ArgentinaFil: Jolly, Ana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; ArgentinaFil: Minatel, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Patología; ArgentinaFil: Hajos, Silvia Elvira. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Inmunología; ArgentinaFil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Bacteriología. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Mundo, Silvia Leonor. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; ArgentinaElsevier2019-04-25T12:46:45Z2019-04-25T12:46:45Z2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0165242716300678http://hdl.handle.net/20.500.12123/49800165-2427https://doi.org/10.1016/j.vetimm.2016.04.010Veterinary Immunology and Immunopathology 176 : 28-33 (August 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:38Zoai:localhost:20.500.12123/4980instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:39.075INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Immunological findings associated with Argentinean strains of Mycobacterium avium subsp. paratuberculosis in bovine models
title Immunological findings associated with Argentinean strains of Mycobacterium avium subsp. paratuberculosis in bovine models
spellingShingle Immunological findings associated with Argentinean strains of Mycobacterium avium subsp. paratuberculosis in bovine models
Colavecchia, Silvia Beatriz
Mycobacterium avium subsp. paratuberculosis
Enfermedades de los Animales
Inmunología
Respuesta Inmunológica
Ternero
Animal Diseases
Immunology
Immune Response
Calves
title_short Immunological findings associated with Argentinean strains of Mycobacterium avium subsp. paratuberculosis in bovine models
title_full Immunological findings associated with Argentinean strains of Mycobacterium avium subsp. paratuberculosis in bovine models
title_fullStr Immunological findings associated with Argentinean strains of Mycobacterium avium subsp. paratuberculosis in bovine models
title_full_unstemmed Immunological findings associated with Argentinean strains of Mycobacterium avium subsp. paratuberculosis in bovine models
title_sort Immunological findings associated with Argentinean strains of Mycobacterium avium subsp. paratuberculosis in bovine models
dc.creator.none.fl_str_mv Colavecchia, Silvia Beatriz
Fernández, Bárbara
Jolly, Ana
Minatel, Leonardo
Hajos, Silvia Elvira
Paolicchi, Fernando
Mundo, Silvia Leonor
author Colavecchia, Silvia Beatriz
author_facet Colavecchia, Silvia Beatriz
Fernández, Bárbara
Jolly, Ana
Minatel, Leonardo
Hajos, Silvia Elvira
Paolicchi, Fernando
Mundo, Silvia Leonor
author_role author
author2 Fernández, Bárbara
Jolly, Ana
Minatel, Leonardo
Hajos, Silvia Elvira
Paolicchi, Fernando
Mundo, Silvia Leonor
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Mycobacterium avium subsp. paratuberculosis
Enfermedades de los Animales
Inmunología
Respuesta Inmunológica
Ternero
Animal Diseases
Immunology
Immune Response
Calves
topic Mycobacterium avium subsp. paratuberculosis
Enfermedades de los Animales
Inmunología
Respuesta Inmunológica
Ternero
Animal Diseases
Immunology
Immune Response
Calves
dc.description.none.fl_txt_mv Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of ruminant paratuberculosis. The aim of this study was to evaluate the biological behavior of different Argentinean strains of MAP in two bovine infection models: macrophage (in vitro) and calf (in vivo) through the evaluation of early immune responses at the peripheral and local levels. Two MAP strains (A and C) were selected taking into account the different patterns of TNF-α and IL-10 secretion displayed by infected bovine macrophages in vitro. Two groups of calves were infected with 250 mg of total wet weight live MAP: strain A infected group (MA, n = 3), strain C infected group (MC, n = 2). Another group of animals was mock-infected (MI, n = 3). Infection was confirmed by MAP culture of feces and microscopic observation of granulomatous lesions in the gut tissue. All infected calves showed positive results in the DTH skin test. A significant increase in peripheral CD4CD25+ cells in MC group on day 150 was detected. The specific cellular immune response developed allowed the identification of the infection as early as 30 days in the MA group. However, the percentage of CD8CD25+ cells was significantly increased on day 120 in MC group. Significant differences between groups in proliferation and cellular responses were also detected in ileocecal lymph node samples. In summary, the strains of MAP employed herein induced differential immune responses in peripheral cells, in the proliferative responses and in cell functionality at the local level. Our findings support the hypotheses that the in vitro behavior displayed by macrophages could be a tool to identify differences among MAP strains infecting bovines and that the host-pathogen interactions occurring upon infection are dependent on the strain of MAP involved.
EEA Balcarce
Fil: Colavecchia, Silvia Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina
Fil: Fernández, Bárbara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina
Fil: Jolly, Ana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina
Fil: Minatel, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Patología; Argentina
Fil: Hajos, Silvia Elvira. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Inmunología; Argentina
Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Bacteriología. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Mundo, Silvia Leonor. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina
description Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of ruminant paratuberculosis. The aim of this study was to evaluate the biological behavior of different Argentinean strains of MAP in two bovine infection models: macrophage (in vitro) and calf (in vivo) through the evaluation of early immune responses at the peripheral and local levels. Two MAP strains (A and C) were selected taking into account the different patterns of TNF-α and IL-10 secretion displayed by infected bovine macrophages in vitro. Two groups of calves were infected with 250 mg of total wet weight live MAP: strain A infected group (MA, n = 3), strain C infected group (MC, n = 2). Another group of animals was mock-infected (MI, n = 3). Infection was confirmed by MAP culture of feces and microscopic observation of granulomatous lesions in the gut tissue. All infected calves showed positive results in the DTH skin test. A significant increase in peripheral CD4CD25+ cells in MC group on day 150 was detected. The specific cellular immune response developed allowed the identification of the infection as early as 30 days in the MA group. However, the percentage of CD8CD25+ cells was significantly increased on day 120 in MC group. Significant differences between groups in proliferation and cellular responses were also detected in ileocecal lymph node samples. In summary, the strains of MAP employed herein induced differential immune responses in peripheral cells, in the proliferative responses and in cell functionality at the local level. Our findings support the hypotheses that the in vitro behavior displayed by macrophages could be a tool to identify differences among MAP strains infecting bovines and that the host-pathogen interactions occurring upon infection are dependent on the strain of MAP involved.
publishDate 2016
dc.date.none.fl_str_mv 2016-08
2019-04-25T12:46:45Z
2019-04-25T12:46:45Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0165242716300678
http://hdl.handle.net/20.500.12123/4980
0165-2427
https://doi.org/10.1016/j.vetimm.2016.04.010
url https://www.sciencedirect.com/science/article/pii/S0165242716300678
http://hdl.handle.net/20.500.12123/4980
https://doi.org/10.1016/j.vetimm.2016.04.010
identifier_str_mv 0165-2427
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Immunology and Immunopathology 176 : 28-33 (August 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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