Modeling tobacco mosaic virus proliferation in protoplasts

Autores
Merchuk, Jose C.; Sánchez Mirón, Asterio; Asurmendi, Sebastian; Shacham, Mordechai
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The tobacco mosaic virus (TMV) is one of the most studied viruses. It is frequently used as a model in the research of virus-host interactions. The interest in understanding the mechanism of its proliferation stems basically from the field of agriculture, due to the detrimental effect this virus has on several crops. In addition to this direct application, virus-mediated protein expression systems, which are well established for the synthesis of foreign proteins in animal cell cultures, are now being applied to plants and plant cells by means of plant viral vectors. The use of transformed roots for the propagation of viral vectors has also been proposed. This work presents a mechanistic model describing the transient process of TMV multiplication in a protoplast (a wall-deprived cell). It aims to be a mathematical tool able to simulate the transient behavior of the main molecular pools taking part in the process, which will be useful for exploring, understanding and predicting the dynamics of a host-virus system. The variables considered are the pools of the main molecules taking part in the viral replication process. The basic balance equations for the cellular pools are presented and a satisfactory fit of the model to the experimental data is shown. The presented model is a necessary step toward the formulation of a basic mechanistic model for the systemic propagation of the virus in a plant tissue. It may be extended in many directions as to the optimization of a system for the production of a foreign protein, to the simulation of manipulation of the virus-cell interaction by external factors, to the mechanism of gene silencing or to the prediction of co-infection dynamics.
Fil: Merchuk, Jose C. Ben-Gurion University of the Negev; Israel
Fil: Sánchez-Mirón, Asterio. Universidad de Almería; España
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Shacham, Mordechai. Ben-Gurion University of the Negev; Israel
Fuente
International journal of biology and biomedical engineering 10 : 202-210. (2016)
Materia
Virus de las Plantas
Nicotiana Tabacum
Tabaco
Modelos de Simulación
Virosis
Viroses
Viruses
Simulation Models
Tobacco
Plant Viruses
Virus del Mosaico del Tabaco
Virus Propagation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2161

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oai_identifier_str oai:localhost:20.500.12123/2161
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spelling Modeling tobacco mosaic virus proliferation in protoplastsMerchuk, Jose C.Sánchez Mirón, AsterioAsurmendi, SebastianShacham, MordechaiVirus de las PlantasNicotiana TabacumTabacoModelos de SimulaciónVirosisVirosesVirusesSimulation ModelsTobaccoPlant VirusesVirus del Mosaico del TabacoVirus PropagationThe tobacco mosaic virus (TMV) is one of the most studied viruses. It is frequently used as a model in the research of virus-host interactions. The interest in understanding the mechanism of its proliferation stems basically from the field of agriculture, due to the detrimental effect this virus has on several crops. In addition to this direct application, virus-mediated protein expression systems, which are well established for the synthesis of foreign proteins in animal cell cultures, are now being applied to plants and plant cells by means of plant viral vectors. The use of transformed roots for the propagation of viral vectors has also been proposed. This work presents a mechanistic model describing the transient process of TMV multiplication in a protoplast (a wall-deprived cell). It aims to be a mathematical tool able to simulate the transient behavior of the main molecular pools taking part in the process, which will be useful for exploring, understanding and predicting the dynamics of a host-virus system. The variables considered are the pools of the main molecules taking part in the viral replication process. The basic balance equations for the cellular pools are presented and a satisfactory fit of the model to the experimental data is shown. The presented model is a necessary step toward the formulation of a basic mechanistic model for the systemic propagation of the virus in a plant tissue. It may be extended in many directions as to the optimization of a system for the production of a foreign protein, to the simulation of manipulation of the virus-cell interaction by external factors, to the mechanism of gene silencing or to the prediction of co-infection dynamics.Fil: Merchuk, Jose C. Ben-Gurion University of the Negev; IsraelFil: Sánchez-Mirón, Asterio. Universidad de Almería; EspañaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Shacham, Mordechai. Ben-Gurion University of the Negev; Israel2018-04-04T13:07:50Z2018-04-04T13:07:50Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2161http://www.naun.org/main/NAUN/bio/2016/a462010-066.pdf1998-4510International journal of biology and biomedical engineering 10 : 202-210. (2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:17Zoai:localhost:20.500.12123/2161instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:17.35INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Modeling tobacco mosaic virus proliferation in protoplasts
title Modeling tobacco mosaic virus proliferation in protoplasts
spellingShingle Modeling tobacco mosaic virus proliferation in protoplasts
Merchuk, Jose C.
Virus de las Plantas
Nicotiana Tabacum
Tabaco
Modelos de Simulación
Virosis
Viroses
Viruses
Simulation Models
Tobacco
Plant Viruses
Virus del Mosaico del Tabaco
Virus Propagation
title_short Modeling tobacco mosaic virus proliferation in protoplasts
title_full Modeling tobacco mosaic virus proliferation in protoplasts
title_fullStr Modeling tobacco mosaic virus proliferation in protoplasts
title_full_unstemmed Modeling tobacco mosaic virus proliferation in protoplasts
title_sort Modeling tobacco mosaic virus proliferation in protoplasts
dc.creator.none.fl_str_mv Merchuk, Jose C.
Sánchez Mirón, Asterio
Asurmendi, Sebastian
Shacham, Mordechai
author Merchuk, Jose C.
author_facet Merchuk, Jose C.
Sánchez Mirón, Asterio
Asurmendi, Sebastian
Shacham, Mordechai
author_role author
author2 Sánchez Mirón, Asterio
Asurmendi, Sebastian
Shacham, Mordechai
author2_role author
author
author
dc.subject.none.fl_str_mv Virus de las Plantas
Nicotiana Tabacum
Tabaco
Modelos de Simulación
Virosis
Viroses
Viruses
Simulation Models
Tobacco
Plant Viruses
Virus del Mosaico del Tabaco
Virus Propagation
topic Virus de las Plantas
Nicotiana Tabacum
Tabaco
Modelos de Simulación
Virosis
Viroses
Viruses
Simulation Models
Tobacco
Plant Viruses
Virus del Mosaico del Tabaco
Virus Propagation
dc.description.none.fl_txt_mv The tobacco mosaic virus (TMV) is one of the most studied viruses. It is frequently used as a model in the research of virus-host interactions. The interest in understanding the mechanism of its proliferation stems basically from the field of agriculture, due to the detrimental effect this virus has on several crops. In addition to this direct application, virus-mediated protein expression systems, which are well established for the synthesis of foreign proteins in animal cell cultures, are now being applied to plants and plant cells by means of plant viral vectors. The use of transformed roots for the propagation of viral vectors has also been proposed. This work presents a mechanistic model describing the transient process of TMV multiplication in a protoplast (a wall-deprived cell). It aims to be a mathematical tool able to simulate the transient behavior of the main molecular pools taking part in the process, which will be useful for exploring, understanding and predicting the dynamics of a host-virus system. The variables considered are the pools of the main molecules taking part in the viral replication process. The basic balance equations for the cellular pools are presented and a satisfactory fit of the model to the experimental data is shown. The presented model is a necessary step toward the formulation of a basic mechanistic model for the systemic propagation of the virus in a plant tissue. It may be extended in many directions as to the optimization of a system for the production of a foreign protein, to the simulation of manipulation of the virus-cell interaction by external factors, to the mechanism of gene silencing or to the prediction of co-infection dynamics.
Fil: Merchuk, Jose C. Ben-Gurion University of the Negev; Israel
Fil: Sánchez-Mirón, Asterio. Universidad de Almería; España
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Shacham, Mordechai. Ben-Gurion University of the Negev; Israel
description The tobacco mosaic virus (TMV) is one of the most studied viruses. It is frequently used as a model in the research of virus-host interactions. The interest in understanding the mechanism of its proliferation stems basically from the field of agriculture, due to the detrimental effect this virus has on several crops. In addition to this direct application, virus-mediated protein expression systems, which are well established for the synthesis of foreign proteins in animal cell cultures, are now being applied to plants and plant cells by means of plant viral vectors. The use of transformed roots for the propagation of viral vectors has also been proposed. This work presents a mechanistic model describing the transient process of TMV multiplication in a protoplast (a wall-deprived cell). It aims to be a mathematical tool able to simulate the transient behavior of the main molecular pools taking part in the process, which will be useful for exploring, understanding and predicting the dynamics of a host-virus system. The variables considered are the pools of the main molecules taking part in the viral replication process. The basic balance equations for the cellular pools are presented and a satisfactory fit of the model to the experimental data is shown. The presented model is a necessary step toward the formulation of a basic mechanistic model for the systemic propagation of the virus in a plant tissue. It may be extended in many directions as to the optimization of a system for the production of a foreign protein, to the simulation of manipulation of the virus-cell interaction by external factors, to the mechanism of gene silencing or to the prediction of co-infection dynamics.
publishDate 2016
dc.date.none.fl_str_mv 2016
2018-04-04T13:07:50Z
2018-04-04T13:07:50Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2161
http://www.naun.org/main/NAUN/bio/2016/a462010-066.pdf
1998-4510
url http://hdl.handle.net/20.500.12123/2161
http://www.naun.org/main/NAUN/bio/2016/a462010-066.pdf
identifier_str_mv 1998-4510
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv International journal of biology and biomedical engineering 10 : 202-210. (2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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