Transgenic expression of tobacco mosaic virus capsid and movement proteins modulate plant basal defense and biotic stress responses in Nicotiana tabacum

Autores
Conti, Gabriela; Rodriguez, Maria Cecilia; Manacorda, Carlos Augusto; Asurmendi, Sebastian
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant viruses cause metabolic and physiological changes associated with symptomatic disease phenotypes. Symptoms involve direct and indirect effects, which result in disruption of host physiology. We used transgenic tobacco expressing a variant of Tobacco mosaic virus (TMV) coat protein (CPT42W) or movement protein (MP), and a hybrid line (MP×CPT42W) that coexpresses both proteins, to study the plant response to individual viral proteins. Findings employing microarray analysis of MP×CPT42W plants and silenced mp×cpT42W* controls revealed that altered transcripts were mostly downregulated, suggesting a persistent shut-off due to MP×CPT42W expression. Next, we showed that MP triggered reactive oxygen species (ROS) accumulation, reduction of total ascorbate, and expression of ROS scavenging genes. These effects were enhanced when both proteins were coexpressed. MP and MP×CPT42W plants showed increased levels of salicylic acid (SA) and SA-responsive gene expression. Furthermore, these effects were partially reproduced in Nicotiana benthamiana when GMP1 transcript was silenced. CPT42W seems to be playing a negative role in the defense response by reducing the expression of PR-1 and RDR-1. MP and MP×CPT42W transgenic expression promoted a recoverylike phenotype in TMV RNA infections and enhanced susceptibility to Pseudomonas syringae and Sclerotinia sclerotiorum. The individual effects of viral proteins may reflect the ability of a virus to balance its own virulence.
Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Molecular plant-microbe interactions 25 (10) : 1370–1384. (2012)
Materia
Nicotiana Tabacum
Tabaco
Proteínas Virales
Estrés Biótico
Virus de las Plantas
ARN
RNA
Plant Viruses
Tobacco
Tobacco Mosaic Virus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2181
Acceder
id INTADig_21024ffa6d3158ab5cc000aab679c0cb
oai_identifier_str oai:localhost:20.500.12123/2181
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Transgenic expression of tobacco mosaic virus capsid and movement proteins modulate plant basal defense and biotic stress responses in Nicotiana tabacumConti, GabrielaRodriguez, Maria CeciliaManacorda, Carlos AugustoAsurmendi, SebastianNicotiana TabacumTabacoProteínas ViralesEstrés BióticoVirus de las PlantasARNRNAPlant VirusesTobaccoTobacco Mosaic VirusPlant viruses cause metabolic and physiological changes associated with symptomatic disease phenotypes. Symptoms involve direct and indirect effects, which result in disruption of host physiology. We used transgenic tobacco expressing a variant of Tobacco mosaic virus (TMV) coat protein (CPT42W) or movement protein (MP), and a hybrid line (MP×CPT42W) that coexpresses both proteins, to study the plant response to individual viral proteins. Findings employing microarray analysis of MP×CPT42W plants and silenced mp×cpT42W* controls revealed that altered transcripts were mostly downregulated, suggesting a persistent shut-off due to MP×CPT42W expression. Next, we showed that MP triggered reactive oxygen species (ROS) accumulation, reduction of total ascorbate, and expression of ROS scavenging genes. These effects were enhanced when both proteins were coexpressed. MP and MP×CPT42W plants showed increased levels of salicylic acid (SA) and SA-responsive gene expression. Furthermore, these effects were partially reproduced in Nicotiana benthamiana when GMP1 transcript was silenced. CPT42W seems to be playing a negative role in the defense response by reducing the expression of PR-1 and RDR-1. MP and MP×CPT42W transgenic expression promoted a recoverylike phenotype in TMV RNA infections and enhanced susceptibility to Pseudomonas syringae and Sclerotinia sclerotiorum. The individual effects of viral proteins may reflect the ability of a virus to balance its own virulence.Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rodriguez, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2018-04-05T16:36:04Z2018-04-05T16:36:04Z2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2181https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI-03-12-0075-R0894-02821943-7706 (online version)https://doi.org/10.1094/MPMI-03-12-0075-RMolecular plant-microbe interactions 25 (10) : 1370–1384. (2012)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:08Zoai:localhost:20.500.12123/2181instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:08.889INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Transgenic expression of tobacco mosaic virus capsid and movement proteins modulate plant basal defense and biotic stress responses in Nicotiana tabacum
title Transgenic expression of tobacco mosaic virus capsid and movement proteins modulate plant basal defense and biotic stress responses in Nicotiana tabacum
spellingShingle Transgenic expression of tobacco mosaic virus capsid and movement proteins modulate plant basal defense and biotic stress responses in Nicotiana tabacum
Conti, Gabriela
Nicotiana Tabacum
Tabaco
Proteínas Virales
Estrés Biótico
Virus de las Plantas
ARN
RNA
Plant Viruses
Tobacco
Tobacco Mosaic Virus
title_short Transgenic expression of tobacco mosaic virus capsid and movement proteins modulate plant basal defense and biotic stress responses in Nicotiana tabacum
title_full Transgenic expression of tobacco mosaic virus capsid and movement proteins modulate plant basal defense and biotic stress responses in Nicotiana tabacum
title_fullStr Transgenic expression of tobacco mosaic virus capsid and movement proteins modulate plant basal defense and biotic stress responses in Nicotiana tabacum
title_full_unstemmed Transgenic expression of tobacco mosaic virus capsid and movement proteins modulate plant basal defense and biotic stress responses in Nicotiana tabacum
title_sort Transgenic expression of tobacco mosaic virus capsid and movement proteins modulate plant basal defense and biotic stress responses in Nicotiana tabacum
dc.creator.none.fl_str_mv Conti, Gabriela
Rodriguez, Maria Cecilia
Manacorda, Carlos Augusto
Asurmendi, Sebastian
author Conti, Gabriela
author_facet Conti, Gabriela
Rodriguez, Maria Cecilia
Manacorda, Carlos Augusto
Asurmendi, Sebastian
author_role author
author2 Rodriguez, Maria Cecilia
Manacorda, Carlos Augusto
Asurmendi, Sebastian
author2_role author
author
author
dc.subject.none.fl_str_mv Nicotiana Tabacum
Tabaco
Proteínas Virales
Estrés Biótico
Virus de las Plantas
ARN
RNA
Plant Viruses
Tobacco
Tobacco Mosaic Virus
topic Nicotiana Tabacum
Tabaco
Proteínas Virales
Estrés Biótico
Virus de las Plantas
ARN
RNA
Plant Viruses
Tobacco
Tobacco Mosaic Virus
dc.description.none.fl_txt_mv Plant viruses cause metabolic and physiological changes associated with symptomatic disease phenotypes. Symptoms involve direct and indirect effects, which result in disruption of host physiology. We used transgenic tobacco expressing a variant of Tobacco mosaic virus (TMV) coat protein (CPT42W) or movement protein (MP), and a hybrid line (MP×CPT42W) that coexpresses both proteins, to study the plant response to individual viral proteins. Findings employing microarray analysis of MP×CPT42W plants and silenced mp×cpT42W* controls revealed that altered transcripts were mostly downregulated, suggesting a persistent shut-off due to MP×CPT42W expression. Next, we showed that MP triggered reactive oxygen species (ROS) accumulation, reduction of total ascorbate, and expression of ROS scavenging genes. These effects were enhanced when both proteins were coexpressed. MP and MP×CPT42W plants showed increased levels of salicylic acid (SA) and SA-responsive gene expression. Furthermore, these effects were partially reproduced in Nicotiana benthamiana when GMP1 transcript was silenced. CPT42W seems to be playing a negative role in the defense response by reducing the expression of PR-1 and RDR-1. MP and MP×CPT42W transgenic expression promoted a recoverylike phenotype in TMV RNA infections and enhanced susceptibility to Pseudomonas syringae and Sclerotinia sclerotiorum. The individual effects of viral proteins may reflect the ability of a virus to balance its own virulence.
Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Plant viruses cause metabolic and physiological changes associated with symptomatic disease phenotypes. Symptoms involve direct and indirect effects, which result in disruption of host physiology. We used transgenic tobacco expressing a variant of Tobacco mosaic virus (TMV) coat protein (CPT42W) or movement protein (MP), and a hybrid line (MP×CPT42W) that coexpresses both proteins, to study the plant response to individual viral proteins. Findings employing microarray analysis of MP×CPT42W plants and silenced mp×cpT42W* controls revealed that altered transcripts were mostly downregulated, suggesting a persistent shut-off due to MP×CPT42W expression. Next, we showed that MP triggered reactive oxygen species (ROS) accumulation, reduction of total ascorbate, and expression of ROS scavenging genes. These effects were enhanced when both proteins were coexpressed. MP and MP×CPT42W plants showed increased levels of salicylic acid (SA) and SA-responsive gene expression. Furthermore, these effects were partially reproduced in Nicotiana benthamiana when GMP1 transcript was silenced. CPT42W seems to be playing a negative role in the defense response by reducing the expression of PR-1 and RDR-1. MP and MP×CPT42W transgenic expression promoted a recoverylike phenotype in TMV RNA infections and enhanced susceptibility to Pseudomonas syringae and Sclerotinia sclerotiorum. The individual effects of viral proteins may reflect the ability of a virus to balance its own virulence.
publishDate 2012
dc.date.none.fl_str_mv 2012
2018-04-05T16:36:04Z
2018-04-05T16:36:04Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2181
https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI-03-12-0075-R
0894-0282
1943-7706 (online version)
https://doi.org/10.1094/MPMI-03-12-0075-R
url http://hdl.handle.net/20.500.12123/2181
https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI-03-12-0075-R
https://doi.org/10.1094/MPMI-03-12-0075-R
identifier_str_mv 0894-0282
1943-7706 (online version)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Molecular plant-microbe interactions 25 (10) : 1370–1384. (2012)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1846143499601182720
score 12.712165

Publicaciones similares