Modeling tobacco mosaic virus proliferation in protoplasts

Autores
Merchuk, José C.; Sánchez Mirón, Asterio; Asurmendi, Sebastian; Shacham, Mordechai
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The tobacco mosaic virus (TMV) is one of the most studied viruses. It is frequently used as a model in the research of virus-host interactions. The interest in understanding the mechanism of its proliferation stems basically from the field of agriculture, due to the detrimental effect this virus has on several crops. In addition to this direct application, virus-mediated protein expression systems, which are well established for the synthesis of foreign proteins in animal cell cultures, are now being applied to plants and plant cells by means of plant viral vectors. The use of transformed roots for the propagation of viral vectors has also been proposed. This work presents a mechanistic model describing the transient process of TMV multiplication in a protoplast (a wall-deprived cell). It aims to be a mathematical tool able to simulate the transient behavior of the main molecular pools taking part in the process, which will be useful for exploring, understanding and predicting the dynamics of a hostvirus system. The variables considered are the pools of the main molecules taking part in the viral replication process. The basic balance equations for the cellular pools are presented and a satisfactory fit of the model to the experimental data is shown. The presented model is a necessary step toward the formulation of a basic mechanistic model for the systemic propagation of the virus in a plant tissue. It may be extended in many directions as to the optimization of a system for the production of a foreign protein, to the simulation of manipulation of the virus-cell interaction by external factors, to the mechanism of gene silencing or to the prediction of co-infection dynamics.
Fil: Merchuk, José C.. Ben Gurion University of the Negev; Israel
Fil: Sánchez Mirón, Asterio. Universidad de Almería.; España
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Shacham, Mordechai. Ben Gurion University of the Negev; Israel
Materia
Tobacco mosaic virus
mechanistic model
simulation
virus propagation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/106626
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Modeling tobacco mosaic virus proliferation in protoplastsMerchuk, José C.Sánchez Mirón, AsterioAsurmendi, SebastianShacham, MordechaiTobacco mosaic virusmechanistic modelsimulationvirus propagationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The tobacco mosaic virus (TMV) is one of the most studied viruses. It is frequently used as a model in the research of virus-host interactions. The interest in understanding the mechanism of its proliferation stems basically from the field of agriculture, due to the detrimental effect this virus has on several crops. In addition to this direct application, virus-mediated protein expression systems, which are well established for the synthesis of foreign proteins in animal cell cultures, are now being applied to plants and plant cells by means of plant viral vectors. The use of transformed roots for the propagation of viral vectors has also been proposed. This work presents a mechanistic model describing the transient process of TMV multiplication in a protoplast (a wall-deprived cell). It aims to be a mathematical tool able to simulate the transient behavior of the main molecular pools taking part in the process, which will be useful for exploring, understanding and predicting the dynamics of a hostvirus system. The variables considered are the pools of the main molecules taking part in the viral replication process. The basic balance equations for the cellular pools are presented and a satisfactory fit of the model to the experimental data is shown. The presented model is a necessary step toward the formulation of a basic mechanistic model for the systemic propagation of the virus in a plant tissue. It may be extended in many directions as to the optimization of a system for the production of a foreign protein, to the simulation of manipulation of the virus-cell interaction by external factors, to the mechanism of gene silencing or to the prediction of co-infection dynamics.Fil: Merchuk, José C.. Ben Gurion University of the Negev; IsraelFil: Sánchez Mirón, Asterio. Universidad de Almería.; EspañaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Shacham, Mordechai. Ben Gurion University of the Negev; IsraelNorth Atlantic University Union2016-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/106626Merchuk, José C.; Sánchez Mirón, Asterio; Asurmendi, Sebastian; Shacham, Mordechai; Modeling tobacco mosaic virus proliferation in protoplasts; North Atlantic University Union; International Journal of Biology and Biomedical Engineering; 10; 6-2016; 202-2101998-4510CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.naun.org/main/NAUN/bio/2016/a462010-066.pdfinfo:eu-repo/semantics/altIdentifier/url/https://repositorio.inta.gob.ar/handle/20.500.12123/2161info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:42:35Zoai:ri.conicet.gov.ar:11336/106626instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:35.879CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modeling tobacco mosaic virus proliferation in protoplasts
title Modeling tobacco mosaic virus proliferation in protoplasts
spellingShingle Modeling tobacco mosaic virus proliferation in protoplasts
Merchuk, José C.
Tobacco mosaic virus
mechanistic model
simulation
virus propagation
title_short Modeling tobacco mosaic virus proliferation in protoplasts
title_full Modeling tobacco mosaic virus proliferation in protoplasts
title_fullStr Modeling tobacco mosaic virus proliferation in protoplasts
title_full_unstemmed Modeling tobacco mosaic virus proliferation in protoplasts
title_sort Modeling tobacco mosaic virus proliferation in protoplasts
dc.creator.none.fl_str_mv Merchuk, José C.
Sánchez Mirón, Asterio
Asurmendi, Sebastian
Shacham, Mordechai
author Merchuk, José C.
author_facet Merchuk, José C.
Sánchez Mirón, Asterio
Asurmendi, Sebastian
Shacham, Mordechai
author_role author
author2 Sánchez Mirón, Asterio
Asurmendi, Sebastian
Shacham, Mordechai
author2_role author
author
author
dc.subject.none.fl_str_mv Tobacco mosaic virus
mechanistic model
simulation
virus propagation
topic Tobacco mosaic virus
mechanistic model
simulation
virus propagation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The tobacco mosaic virus (TMV) is one of the most studied viruses. It is frequently used as a model in the research of virus-host interactions. The interest in understanding the mechanism of its proliferation stems basically from the field of agriculture, due to the detrimental effect this virus has on several crops. In addition to this direct application, virus-mediated protein expression systems, which are well established for the synthesis of foreign proteins in animal cell cultures, are now being applied to plants and plant cells by means of plant viral vectors. The use of transformed roots for the propagation of viral vectors has also been proposed. This work presents a mechanistic model describing the transient process of TMV multiplication in a protoplast (a wall-deprived cell). It aims to be a mathematical tool able to simulate the transient behavior of the main molecular pools taking part in the process, which will be useful for exploring, understanding and predicting the dynamics of a hostvirus system. The variables considered are the pools of the main molecules taking part in the viral replication process. The basic balance equations for the cellular pools are presented and a satisfactory fit of the model to the experimental data is shown. The presented model is a necessary step toward the formulation of a basic mechanistic model for the systemic propagation of the virus in a plant tissue. It may be extended in many directions as to the optimization of a system for the production of a foreign protein, to the simulation of manipulation of the virus-cell interaction by external factors, to the mechanism of gene silencing or to the prediction of co-infection dynamics.
Fil: Merchuk, José C.. Ben Gurion University of the Negev; Israel
Fil: Sánchez Mirón, Asterio. Universidad de Almería.; España
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Shacham, Mordechai. Ben Gurion University of the Negev; Israel
description The tobacco mosaic virus (TMV) is one of the most studied viruses. It is frequently used as a model in the research of virus-host interactions. The interest in understanding the mechanism of its proliferation stems basically from the field of agriculture, due to the detrimental effect this virus has on several crops. In addition to this direct application, virus-mediated protein expression systems, which are well established for the synthesis of foreign proteins in animal cell cultures, are now being applied to plants and plant cells by means of plant viral vectors. The use of transformed roots for the propagation of viral vectors has also been proposed. This work presents a mechanistic model describing the transient process of TMV multiplication in a protoplast (a wall-deprived cell). It aims to be a mathematical tool able to simulate the transient behavior of the main molecular pools taking part in the process, which will be useful for exploring, understanding and predicting the dynamics of a hostvirus system. The variables considered are the pools of the main molecules taking part in the viral replication process. The basic balance equations for the cellular pools are presented and a satisfactory fit of the model to the experimental data is shown. The presented model is a necessary step toward the formulation of a basic mechanistic model for the systemic propagation of the virus in a plant tissue. It may be extended in many directions as to the optimization of a system for the production of a foreign protein, to the simulation of manipulation of the virus-cell interaction by external factors, to the mechanism of gene silencing or to the prediction of co-infection dynamics.
publishDate 2016
dc.date.none.fl_str_mv 2016-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/106626
Merchuk, José C.; Sánchez Mirón, Asterio; Asurmendi, Sebastian; Shacham, Mordechai; Modeling tobacco mosaic virus proliferation in protoplasts; North Atlantic University Union; International Journal of Biology and Biomedical Engineering; 10; 6-2016; 202-210
1998-4510
CONICET Digital
CONICET
url http://hdl.handle.net/11336/106626
identifier_str_mv Merchuk, José C.; Sánchez Mirón, Asterio; Asurmendi, Sebastian; Shacham, Mordechai; Modeling tobacco mosaic virus proliferation in protoplasts; North Atlantic University Union; International Journal of Biology and Biomedical Engineering; 10; 6-2016; 202-210
1998-4510
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.naun.org/main/NAUN/bio/2016/a462010-066.pdf
info:eu-repo/semantics/altIdentifier/url/https://repositorio.inta.gob.ar/handle/20.500.12123/2161
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv North Atlantic University Union
publisher.none.fl_str_mv North Atlantic University Union
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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