Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes

Autores
Bazzini, Ariel Alejandro; Manacorda, Carlos Augusto; Tohge, Takayuki; Conti, Gabriela; Rodriguez, Maria Cecilia; Nunes Nesi, Adriano; Villanueva, Sofía; Fernie, Alisdair R.; Carrari, Fernando; Asurmendi, Sebastian
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in signaling and defense that orchestrate and execute the response in plant-pathogen interactions. We used a systemic approach by applying both liquid and gas chromatography coupled to mass spectrometry to determine the relative level of metabolites across the viral infection, together with a miRs profiling using a micro-array based procedure. Systemic changes in metabolites were characterized by a biphasic response after infection. The first phase, detected at one dpi, evidenced the action of a systemic signal since no virus was detected systemically. Several of the metabolites increased at this stage were hormone-related. miRs profiling after infection also revealed a biphasic alteration, showing miRs alteration at 5 dpi where no virus was detected systemically and a late phase correlating with virus accumulation. Correlation analyses revealed a massive increase in the density of correlation networks after infection indicating a complex reprogramming of the regulatory pathways, either in response to the plant defense mechanism or to the virus infection itself. Our data propose the involvement of a systemic signaling on early miRs alteration.
Fil: Bazzini, Ariel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Tohge, Takayuki. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania
Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Rodriguez, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nunes Nesi, Adriano. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania
Fil: Villanueva, Sofía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Fernie, Alisdair R. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
PLoS ONE 6 (12) :e28466. (December 2011)
Materia
RNA
Metabolismo
Virus de las Plantas
Nicotiana Tabacum
Tabaco
Cromatografía Gas-líquido
Espectrometría de Masas
Mass Spectrometry
Gas Liquid Chromatography
Tobacco
Plant Viruses
Virus del Mosaico del Tabaco
miRNA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2168
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2168
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changesBazzini, Ariel AlejandroManacorda, Carlos AugustoTohge, TakayukiConti, GabrielaRodriguez, Maria CeciliaNunes Nesi, AdrianoVillanueva, SofíaFernie, Alisdair R.Carrari, FernandoAsurmendi, SebastianRNAMetabolismoVirus de las PlantasNicotiana TabacumTabacoCromatografía Gas-líquidoEspectrometría de MasasMass SpectrometryGas Liquid ChromatographyTobaccoPlant VirusesVirus del Mosaico del TabacomiRNAPlant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in signaling and defense that orchestrate and execute the response in plant-pathogen interactions. We used a systemic approach by applying both liquid and gas chromatography coupled to mass spectrometry to determine the relative level of metabolites across the viral infection, together with a miRs profiling using a micro-array based procedure. Systemic changes in metabolites were characterized by a biphasic response after infection. The first phase, detected at one dpi, evidenced the action of a systemic signal since no virus was detected systemically. Several of the metabolites increased at this stage were hormone-related. miRs profiling after infection also revealed a biphasic alteration, showing miRs alteration at 5 dpi where no virus was detected systemically and a late phase correlating with virus accumulation. Correlation analyses revealed a massive increase in the density of correlation networks after infection indicating a complex reprogramming of the regulatory pathways, either in response to the plant defense mechanism or to the virus infection itself. Our data propose the involvement of a systemic signaling on early miRs alteration.Fil: Bazzini, Ariel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Tohge, Takayuki. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; AlemaniaFil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Rodriguez, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nunes Nesi, Adriano. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; AlemaniaFil: Villanueva, Sofía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Fernie, Alisdair R. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; AlemaniaFil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina2018-04-04T18:21:36Z2018-04-04T18:21:36Z2011-12-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2168https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236191/1932-6203PLoS ONE 6 (12) :e28466. (December 2011)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:17Zoai:localhost:20.500.12123/2168instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:17.372INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes
title Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes
spellingShingle Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes
Bazzini, Ariel Alejandro
RNA
Metabolismo
Virus de las Plantas
Nicotiana Tabacum
Tabaco
Cromatografía Gas-líquido
Espectrometría de Masas
Mass Spectrometry
Gas Liquid Chromatography
Tobacco
Plant Viruses
Virus del Mosaico del Tabaco
miRNA
title_short Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes
title_full Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes
title_fullStr Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes
title_full_unstemmed Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes
title_sort Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes
dc.creator.none.fl_str_mv Bazzini, Ariel Alejandro
Manacorda, Carlos Augusto
Tohge, Takayuki
Conti, Gabriela
Rodriguez, Maria Cecilia
Nunes Nesi, Adriano
Villanueva, Sofía
Fernie, Alisdair R.
Carrari, Fernando
Asurmendi, Sebastian
author Bazzini, Ariel Alejandro
author_facet Bazzini, Ariel Alejandro
Manacorda, Carlos Augusto
Tohge, Takayuki
Conti, Gabriela
Rodriguez, Maria Cecilia
Nunes Nesi, Adriano
Villanueva, Sofía
Fernie, Alisdair R.
Carrari, Fernando
Asurmendi, Sebastian
author_role author
author2 Manacorda, Carlos Augusto
Tohge, Takayuki
Conti, Gabriela
Rodriguez, Maria Cecilia
Nunes Nesi, Adriano
Villanueva, Sofía
Fernie, Alisdair R.
Carrari, Fernando
Asurmendi, Sebastian
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv RNA
Metabolismo
Virus de las Plantas
Nicotiana Tabacum
Tabaco
Cromatografía Gas-líquido
Espectrometría de Masas
Mass Spectrometry
Gas Liquid Chromatography
Tobacco
Plant Viruses
Virus del Mosaico del Tabaco
miRNA
topic RNA
Metabolismo
Virus de las Plantas
Nicotiana Tabacum
Tabaco
Cromatografía Gas-líquido
Espectrometría de Masas
Mass Spectrometry
Gas Liquid Chromatography
Tobacco
Plant Viruses
Virus del Mosaico del Tabaco
miRNA
dc.description.none.fl_txt_mv Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in signaling and defense that orchestrate and execute the response in plant-pathogen interactions. We used a systemic approach by applying both liquid and gas chromatography coupled to mass spectrometry to determine the relative level of metabolites across the viral infection, together with a miRs profiling using a micro-array based procedure. Systemic changes in metabolites were characterized by a biphasic response after infection. The first phase, detected at one dpi, evidenced the action of a systemic signal since no virus was detected systemically. Several of the metabolites increased at this stage were hormone-related. miRs profiling after infection also revealed a biphasic alteration, showing miRs alteration at 5 dpi where no virus was detected systemically and a late phase correlating with virus accumulation. Correlation analyses revealed a massive increase in the density of correlation networks after infection indicating a complex reprogramming of the regulatory pathways, either in response to the plant defense mechanism or to the virus infection itself. Our data propose the involvement of a systemic signaling on early miRs alteration.
Fil: Bazzini, Ariel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Tohge, Takayuki. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania
Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Rodriguez, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nunes Nesi, Adriano. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania
Fil: Villanueva, Sofía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Fernie, Alisdair R. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in signaling and defense that orchestrate and execute the response in plant-pathogen interactions. We used a systemic approach by applying both liquid and gas chromatography coupled to mass spectrometry to determine the relative level of metabolites across the viral infection, together with a miRs profiling using a micro-array based procedure. Systemic changes in metabolites were characterized by a biphasic response after infection. The first phase, detected at one dpi, evidenced the action of a systemic signal since no virus was detected systemically. Several of the metabolites increased at this stage were hormone-related. miRs profiling after infection also revealed a biphasic alteration, showing miRs alteration at 5 dpi where no virus was detected systemically and a late phase correlating with virus accumulation. Correlation analyses revealed a massive increase in the density of correlation networks after infection indicating a complex reprogramming of the regulatory pathways, either in response to the plant defense mechanism or to the virus infection itself. Our data propose the involvement of a systemic signaling on early miRs alteration.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-12
2018-04-04T18:21:36Z
2018-04-04T18:21:36Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2168
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236191/
1932-6203
url http://hdl.handle.net/20.500.12123/2168
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236191/
identifier_str_mv 1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv PLoS ONE 6 (12) :e28466. (December 2011)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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