Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds

Autores
Nunes Nesi, Adriano; Alseekh, Saleh; Oliveira Silva, Franklin Magnum de; Omranian, Nooshin; Lichtenstein, Gabriel; Mirnezhad, Mohammad; Romero González, Roman R.; Carrari, Fernando
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Nunes Nesi, Adriano.Universidade Federal de Viçosa. Departamento de Biologia Vegetal. Viçosa, Minas Gerais, Brazil.
Fil: Alseekh, Saleh. Max - Planck- Institute of Molecular Plant Physiology. Potsdam, Germany.
Fil: Oliveira Silva, Franklin Magnum de. Universidade Federal de Viçosa. Departamento de Biologia Vegetal. Viçosa, Minas Gerais, Brazil.
Fil: Omranian, Nooshin. Max - Planck- Institute of Molecular Plant Physiology. Potsdam, Germany.
Fil: Lichtenstein, Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Castelar, Buenos Aires, Argentina.
Fil: Mirnezhad, Mohammad. Leiden University. Plant Ecology, Institute of Biology. The Netherlands.
Fil: Romero González, Roman R. Leiden University. Plant Ecology. Institute of Biology. The Netherlands.
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Castelar, Buenos Aires, Argentina.
Introduction To date, most studies of natural variation and metabolite quantitative trait loci (mQTL) in tomato have focused on fruit metabolism, leaving aside the identification of genomic regions involved in the regulation of leaf metabolism. Objective This study was conducted to identify leaf mQTL in tomato and to assess the association of leaf metabolites and physiological traits with the metabolite levels from other tissues. Methods The analysis of components of leaf metabolism was performed by phenotypying 76 tomato ILs with chromosome segments of the wild species Solanum pennellii in the genetic background of a cultivated tomato (S. lycopersicum) variety M82. The plants were cultivated in two different environments in independent years and samples were harvested from mature leaves of non-flowering plants at the middle of the light period. The non-targeted metabolite profiling was obtained by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). With the data set obtained in this study and already published metabolomics data from seed and fruit, we performed QTL mapping, heritability and correlation analyses. Results Changes in metabolite contents were evident in the ILs that are potentially important with respect to stress responses and plant physiology. By analyzing the obtained data, we identified 42 positive and 76 negative mQTL involved in carbon and nitrogen metabolism. Conclusions Overall, these findings allowed the identification of S. lycopersicum genome regions involved in the regulation of leaf primary carbon and nitrogen metabolism, as well as the association of leaf metabolites with metabolites from seeds and fruits.
grafs., tbls.
Fuente
Metabolomics
Vol.15, no.46
1-13
https://link.springer.com
Materia
METABOLITE QTL
TOMATO
LEAF METABOLISM
METABOLITE NETWORK
Nivel de accesibilidad
acceso abierto
Condiciones de uso
acceso abierto
Repositorio
FAUBA Digital (UBA-FAUBA)
Institución
Universidad de Buenos Aires. Facultad de Agronomía
OAI Identificador
snrd:2019nunesnesi

id FAUBA_5454ac988f8974976138c3bfda583391
oai_identifier_str snrd:2019nunesnesi
network_acronym_str FAUBA
repository_id_str 2729
network_name_str FAUBA Digital (UBA-FAUBA)
spelling Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seedsNunes Nesi, AdrianoAlseekh, SalehOliveira Silva, Franklin Magnum deOmranian, NooshinLichtenstein, GabrielMirnezhad, MohammadRomero González, Roman R.Carrari, FernandoMETABOLITE QTLTOMATOLEAF METABOLISMMETABOLITE NETWORKFil: Nunes Nesi, Adriano.Universidade Federal de Viçosa. Departamento de Biologia Vegetal. Viçosa, Minas Gerais, Brazil.Fil: Alseekh, Saleh. Max - Planck- Institute of Molecular Plant Physiology. Potsdam, Germany.Fil: Oliveira Silva, Franklin Magnum de. Universidade Federal de Viçosa. Departamento de Biologia Vegetal. Viçosa, Minas Gerais, Brazil.Fil: Omranian, Nooshin. Max - Planck- Institute of Molecular Plant Physiology. Potsdam, Germany.Fil: Lichtenstein, Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Castelar, Buenos Aires, Argentina.Fil: Mirnezhad, Mohammad. Leiden University. Plant Ecology, Institute of Biology. The Netherlands.Fil: Romero González, Roman R. Leiden University. Plant Ecology. Institute of Biology. The Netherlands.Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Castelar, Buenos Aires, Argentina.Introduction To date, most studies of natural variation and metabolite quantitative trait loci (mQTL) in tomato have focused on fruit metabolism, leaving aside the identification of genomic regions involved in the regulation of leaf metabolism. Objective This study was conducted to identify leaf mQTL in tomato and to assess the association of leaf metabolites and physiological traits with the metabolite levels from other tissues. Methods The analysis of components of leaf metabolism was performed by phenotypying 76 tomato ILs with chromosome segments of the wild species Solanum pennellii in the genetic background of a cultivated tomato (S. lycopersicum) variety M82. The plants were cultivated in two different environments in independent years and samples were harvested from mature leaves of non-flowering plants at the middle of the light period. The non-targeted metabolite profiling was obtained by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). With the data set obtained in this study and already published metabolomics data from seed and fruit, we performed QTL mapping, heritability and correlation analyses. Results Changes in metabolite contents were evident in the ILs that are potentially important with respect to stress responses and plant physiology. By analyzing the obtained data, we identified 42 positive and 76 negative mQTL involved in carbon and nitrogen metabolism. Conclusions Overall, these findings allowed the identification of S. lycopersicum genome regions involved in the regulation of leaf primary carbon and nitrogen metabolism, as well as the association of leaf metabolites with metabolites from seeds and fruits.grafs., tbls.2019articleinfo:eu-repo/semantics/articlepublishedVersioninfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfdoi:10.1007/s11306-019-1503-8issn:1573-3882http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2019nunesnesiMetabolomicsVol.15, no.461-13https://link.springer.comreponame:FAUBA Digital (UBA-FAUBA)instname:Universidad de Buenos Aires. Facultad de Agronomíaenginfo:eu-repo/semantics/openAccessopenAccesshttp://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section42025-09-04T09:44:38Zsnrd:2019nunesnesiinstacron:UBA-FAUBAInstitucionalhttp://ri.agro.uba.ar/Universidad públicaNo correspondehttp://ri.agro.uba.ar/greenstone3/oaiserver?verb=ListSetsmartino@agro.uba.ar;berasa@agro.uba.ar ArgentinaNo correspondeNo correspondeNo correspondeopendoar:27292025-09-04 09:44:39.369FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomíafalse
dc.title.none.fl_str_mv Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
title Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
spellingShingle Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
Nunes Nesi, Adriano
METABOLITE QTL
TOMATO
LEAF METABOLISM
METABOLITE NETWORK
title_short Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
title_full Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
title_fullStr Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
title_full_unstemmed Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
title_sort Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
dc.creator.none.fl_str_mv Nunes Nesi, Adriano
Alseekh, Saleh
Oliveira Silva, Franklin Magnum de
Omranian, Nooshin
Lichtenstein, Gabriel
Mirnezhad, Mohammad
Romero González, Roman R.
Carrari, Fernando
author Nunes Nesi, Adriano
author_facet Nunes Nesi, Adriano
Alseekh, Saleh
Oliveira Silva, Franklin Magnum de
Omranian, Nooshin
Lichtenstein, Gabriel
Mirnezhad, Mohammad
Romero González, Roman R.
Carrari, Fernando
author_role author
author2 Alseekh, Saleh
Oliveira Silva, Franklin Magnum de
Omranian, Nooshin
Lichtenstein, Gabriel
Mirnezhad, Mohammad
Romero González, Roman R.
Carrari, Fernando
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv METABOLITE QTL
TOMATO
LEAF METABOLISM
METABOLITE NETWORK
topic METABOLITE QTL
TOMATO
LEAF METABOLISM
METABOLITE NETWORK
dc.description.none.fl_txt_mv Fil: Nunes Nesi, Adriano.Universidade Federal de Viçosa. Departamento de Biologia Vegetal. Viçosa, Minas Gerais, Brazil.
Fil: Alseekh, Saleh. Max - Planck- Institute of Molecular Plant Physiology. Potsdam, Germany.
Fil: Oliveira Silva, Franklin Magnum de. Universidade Federal de Viçosa. Departamento de Biologia Vegetal. Viçosa, Minas Gerais, Brazil.
Fil: Omranian, Nooshin. Max - Planck- Institute of Molecular Plant Physiology. Potsdam, Germany.
Fil: Lichtenstein, Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Castelar, Buenos Aires, Argentina.
Fil: Mirnezhad, Mohammad. Leiden University. Plant Ecology, Institute of Biology. The Netherlands.
Fil: Romero González, Roman R. Leiden University. Plant Ecology. Institute of Biology. The Netherlands.
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Castelar, Buenos Aires, Argentina.
Introduction To date, most studies of natural variation and metabolite quantitative trait loci (mQTL) in tomato have focused on fruit metabolism, leaving aside the identification of genomic regions involved in the regulation of leaf metabolism. Objective This study was conducted to identify leaf mQTL in tomato and to assess the association of leaf metabolites and physiological traits with the metabolite levels from other tissues. Methods The analysis of components of leaf metabolism was performed by phenotypying 76 tomato ILs with chromosome segments of the wild species Solanum pennellii in the genetic background of a cultivated tomato (S. lycopersicum) variety M82. The plants were cultivated in two different environments in independent years and samples were harvested from mature leaves of non-flowering plants at the middle of the light period. The non-targeted metabolite profiling was obtained by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). With the data set obtained in this study and already published metabolomics data from seed and fruit, we performed QTL mapping, heritability and correlation analyses. Results Changes in metabolite contents were evident in the ILs that are potentially important with respect to stress responses and plant physiology. By analyzing the obtained data, we identified 42 positive and 76 negative mQTL involved in carbon and nitrogen metabolism. Conclusions Overall, these findings allowed the identification of S. lycopersicum genome regions involved in the regulation of leaf primary carbon and nitrogen metabolism, as well as the association of leaf metabolites with metabolites from seeds and fruits.
grafs., tbls.
description Fil: Nunes Nesi, Adriano.Universidade Federal de Viçosa. Departamento de Biologia Vegetal. Viçosa, Minas Gerais, Brazil.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv article
info:eu-repo/semantics/article
publishedVersion
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv doi:10.1007/s11306-019-1503-8
issn:1573-3882
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2019nunesnesi
identifier_str_mv doi:10.1007/s11306-019-1503-8
issn:1573-3882
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2019nunesnesi
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
eu_rights_str_mv openAccess
rights_invalid_str_mv openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Metabolomics
Vol.15, no.46
1-13
https://link.springer.com
reponame:FAUBA Digital (UBA-FAUBA)
instname:Universidad de Buenos Aires. Facultad de Agronomía
reponame_str FAUBA Digital (UBA-FAUBA)
collection FAUBA Digital (UBA-FAUBA)
instname_str Universidad de Buenos Aires. Facultad de Agronomía
repository.name.fl_str_mv FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomía
repository.mail.fl_str_mv martino@agro.uba.ar;berasa@agro.uba.ar
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