Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
- Autores
- Nunes Nesi, Adriano; Alseekh, Saleh; Oliveira Silva, Franklin Magnum de; Omranian, Nooshin; Lichtenstein, Gabriel; Mirnezhad, Mohammad; Romero González, Roman R.; Carrari, Fernando
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Nunes Nesi, Adriano.Universidade Federal de Viçosa. Departamento de Biologia Vegetal. Viçosa, Minas Gerais, Brazil.
Fil: Alseekh, Saleh. Max - Planck- Institute of Molecular Plant Physiology. Potsdam, Germany.
Fil: Oliveira Silva, Franklin Magnum de. Universidade Federal de Viçosa. Departamento de Biologia Vegetal. Viçosa, Minas Gerais, Brazil.
Fil: Omranian, Nooshin. Max - Planck- Institute of Molecular Plant Physiology. Potsdam, Germany.
Fil: Lichtenstein, Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Castelar, Buenos Aires, Argentina.
Fil: Mirnezhad, Mohammad. Leiden University. Plant Ecology, Institute of Biology. The Netherlands.
Fil: Romero González, Roman R. Leiden University. Plant Ecology. Institute of Biology. The Netherlands.
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Castelar, Buenos Aires, Argentina.
Introduction To date, most studies of natural variation and metabolite quantitative trait loci (mQTL) in tomato have focused on fruit metabolism, leaving aside the identification of genomic regions involved in the regulation of leaf metabolism. Objective This study was conducted to identify leaf mQTL in tomato and to assess the association of leaf metabolites and physiological traits with the metabolite levels from other tissues. Methods The analysis of components of leaf metabolism was performed by phenotypying 76 tomato ILs with chromosome segments of the wild species Solanum pennellii in the genetic background of a cultivated tomato (S. lycopersicum) variety M82. The plants were cultivated in two different environments in independent years and samples were harvested from mature leaves of non-flowering plants at the middle of the light period. The non-targeted metabolite profiling was obtained by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). With the data set obtained in this study and already published metabolomics data from seed and fruit, we performed QTL mapping, heritability and correlation analyses. Results Changes in metabolite contents were evident in the ILs that are potentially important with respect to stress responses and plant physiology. By analyzing the obtained data, we identified 42 positive and 76 negative mQTL involved in carbon and nitrogen metabolism. Conclusions Overall, these findings allowed the identification of S. lycopersicum genome regions involved in the regulation of leaf primary carbon and nitrogen metabolism, as well as the association of leaf metabolites with metabolites from seeds and fruits.
grafs., tbls. - Fuente
- Metabolomics
Vol.15, no.46
1-13
https://link.springer.com - Materia
-
METABOLITE QTL
TOMATO
LEAF METABOLISM
METABOLITE NETWORK - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- acceso abierto
- Repositorio
- Institución
- Universidad de Buenos Aires. Facultad de Agronomía
- OAI Identificador
- snrd:2019nunesnesi
Ver los metadatos del registro completo
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Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seedsNunes Nesi, AdrianoAlseekh, SalehOliveira Silva, Franklin Magnum deOmranian, NooshinLichtenstein, GabrielMirnezhad, MohammadRomero González, Roman R.Carrari, FernandoMETABOLITE QTLTOMATOLEAF METABOLISMMETABOLITE NETWORKFil: Nunes Nesi, Adriano.Universidade Federal de Viçosa. Departamento de Biologia Vegetal. Viçosa, Minas Gerais, Brazil.Fil: Alseekh, Saleh. Max - Planck- Institute of Molecular Plant Physiology. Potsdam, Germany.Fil: Oliveira Silva, Franklin Magnum de. Universidade Federal de Viçosa. Departamento de Biologia Vegetal. Viçosa, Minas Gerais, Brazil.Fil: Omranian, Nooshin. Max - Planck- Institute of Molecular Plant Physiology. Potsdam, Germany.Fil: Lichtenstein, Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Castelar, Buenos Aires, Argentina.Fil: Mirnezhad, Mohammad. Leiden University. Plant Ecology, Institute of Biology. The Netherlands.Fil: Romero González, Roman R. Leiden University. Plant Ecology. Institute of Biology. The Netherlands.Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Castelar, Buenos Aires, Argentina.Introduction To date, most studies of natural variation and metabolite quantitative trait loci (mQTL) in tomato have focused on fruit metabolism, leaving aside the identification of genomic regions involved in the regulation of leaf metabolism. Objective This study was conducted to identify leaf mQTL in tomato and to assess the association of leaf metabolites and physiological traits with the metabolite levels from other tissues. Methods The analysis of components of leaf metabolism was performed by phenotypying 76 tomato ILs with chromosome segments of the wild species Solanum pennellii in the genetic background of a cultivated tomato (S. lycopersicum) variety M82. The plants were cultivated in two different environments in independent years and samples were harvested from mature leaves of non-flowering plants at the middle of the light period. The non-targeted metabolite profiling was obtained by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). With the data set obtained in this study and already published metabolomics data from seed and fruit, we performed QTL mapping, heritability and correlation analyses. Results Changes in metabolite contents were evident in the ILs that are potentially important with respect to stress responses and plant physiology. By analyzing the obtained data, we identified 42 positive and 76 negative mQTL involved in carbon and nitrogen metabolism. Conclusions Overall, these findings allowed the identification of S. lycopersicum genome regions involved in the regulation of leaf primary carbon and nitrogen metabolism, as well as the association of leaf metabolites with metabolites from seeds and fruits.grafs., tbls.2019articleinfo:eu-repo/semantics/articlepublishedVersioninfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfdoi:10.1007/s11306-019-1503-8issn:1573-3882http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2019nunesnesiMetabolomicsVol.15, no.461-13https://link.springer.comreponame:FAUBA Digital (UBA-FAUBA)instname:Universidad de Buenos Aires. Facultad de Agronomíaenginfo:eu-repo/semantics/openAccessopenAccesshttp://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section42025-09-04T09:44:38Zsnrd:2019nunesnesiinstacron:UBA-FAUBAInstitucionalhttp://ri.agro.uba.ar/Universidad públicaNo correspondehttp://ri.agro.uba.ar/greenstone3/oaiserver?verb=ListSetsmartino@agro.uba.ar;berasa@agro.uba.ar ArgentinaNo correspondeNo correspondeNo correspondeopendoar:27292025-09-04 09:44:39.369FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomíafalse |
dc.title.none.fl_str_mv |
Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds |
title |
Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds |
spellingShingle |
Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds Nunes Nesi, Adriano METABOLITE QTL TOMATO LEAF METABOLISM METABOLITE NETWORK |
title_short |
Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds |
title_full |
Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds |
title_fullStr |
Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds |
title_full_unstemmed |
Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds |
title_sort |
Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds |
dc.creator.none.fl_str_mv |
Nunes Nesi, Adriano Alseekh, Saleh Oliveira Silva, Franklin Magnum de Omranian, Nooshin Lichtenstein, Gabriel Mirnezhad, Mohammad Romero González, Roman R. Carrari, Fernando |
author |
Nunes Nesi, Adriano |
author_facet |
Nunes Nesi, Adriano Alseekh, Saleh Oliveira Silva, Franklin Magnum de Omranian, Nooshin Lichtenstein, Gabriel Mirnezhad, Mohammad Romero González, Roman R. Carrari, Fernando |
author_role |
author |
author2 |
Alseekh, Saleh Oliveira Silva, Franklin Magnum de Omranian, Nooshin Lichtenstein, Gabriel Mirnezhad, Mohammad Romero González, Roman R. Carrari, Fernando |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
METABOLITE QTL TOMATO LEAF METABOLISM METABOLITE NETWORK |
topic |
METABOLITE QTL TOMATO LEAF METABOLISM METABOLITE NETWORK |
dc.description.none.fl_txt_mv |
Fil: Nunes Nesi, Adriano.Universidade Federal de Viçosa. Departamento de Biologia Vegetal. Viçosa, Minas Gerais, Brazil. Fil: Alseekh, Saleh. Max - Planck- Institute of Molecular Plant Physiology. Potsdam, Germany. Fil: Oliveira Silva, Franklin Magnum de. Universidade Federal de Viçosa. Departamento de Biologia Vegetal. Viçosa, Minas Gerais, Brazil. Fil: Omranian, Nooshin. Max - Planck- Institute of Molecular Plant Physiology. Potsdam, Germany. Fil: Lichtenstein, Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Castelar, Buenos Aires, Argentina. Fil: Mirnezhad, Mohammad. Leiden University. Plant Ecology, Institute of Biology. The Netherlands. Fil: Romero González, Roman R. Leiden University. Plant Ecology. Institute of Biology. The Netherlands. Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Castelar, Buenos Aires, Argentina. Introduction To date, most studies of natural variation and metabolite quantitative trait loci (mQTL) in tomato have focused on fruit metabolism, leaving aside the identification of genomic regions involved in the regulation of leaf metabolism. Objective This study was conducted to identify leaf mQTL in tomato and to assess the association of leaf metabolites and physiological traits with the metabolite levels from other tissues. Methods The analysis of components of leaf metabolism was performed by phenotypying 76 tomato ILs with chromosome segments of the wild species Solanum pennellii in the genetic background of a cultivated tomato (S. lycopersicum) variety M82. The plants were cultivated in two different environments in independent years and samples were harvested from mature leaves of non-flowering plants at the middle of the light period. The non-targeted metabolite profiling was obtained by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). With the data set obtained in this study and already published metabolomics data from seed and fruit, we performed QTL mapping, heritability and correlation analyses. Results Changes in metabolite contents were evident in the ILs that are potentially important with respect to stress responses and plant physiology. By analyzing the obtained data, we identified 42 positive and 76 negative mQTL involved in carbon and nitrogen metabolism. Conclusions Overall, these findings allowed the identification of S. lycopersicum genome regions involved in the regulation of leaf primary carbon and nitrogen metabolism, as well as the association of leaf metabolites with metabolites from seeds and fruits. grafs., tbls. |
description |
Fil: Nunes Nesi, Adriano.Universidade Federal de Viçosa. Departamento de Biologia Vegetal. Viçosa, Minas Gerais, Brazil. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.none.fl_str_mv |
article info:eu-repo/semantics/article publishedVersion info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
doi:10.1007/s11306-019-1503-8 issn:1573-3882 http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2019nunesnesi |
identifier_str_mv |
doi:10.1007/s11306-019-1503-8 issn:1573-3882 |
url |
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2019nunesnesi |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Metabolomics Vol.15, no.46 1-13 https://link.springer.com reponame:FAUBA Digital (UBA-FAUBA) instname:Universidad de Buenos Aires. Facultad de Agronomía |
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FAUBA Digital (UBA-FAUBA) |
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FAUBA Digital (UBA-FAUBA) |
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Universidad de Buenos Aires. Facultad de Agronomía |
repository.name.fl_str_mv |
FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomía |
repository.mail.fl_str_mv |
martino@agro.uba.ar;berasa@agro.uba.ar |
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