Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds

Autores
Nunes Nesi, Adriano; Alseekh, Saleh; de Oliveira Silva, Franklin Magnum; Omranian, Nooshin; Lichtenstein, Gabriel; Mirnezhad, Mohammad; Romero González, Roman R.; Sabio y Garcia, Julia Veronica; Conte, Mariana; Leiss, Kirsten A.; Klinkhamer, Peter G. L.; Nikoloski, Zoran; Carrari, Fernando Oscar; Fernie, Alisdair R.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: To date, most studies of natural variation and metabolite quantitative trait loci (mQTL) in tomato have focused on fruit metabolism, leaving aside the identification of genomic regions involved in the regulation of leaf metabolism. Objective: This study was conducted to identify leaf mQTL in tomato and to assess the association of leaf metabolites and physiological traits with the metabolite levels from other tissues. Methods: The analysis of components of leaf metabolism was performed by phenotypying 76 tomato ILs with chromosome segments of the wild species Solanum pennellii in the genetic background of a cultivated tomato (S. lycopersicum) variety M82. The plants were cultivated in two different environments in independent years and samples were harvested from mature leaves of non-flowering plants at the middle of the light period. The non-targeted metabolite profiling was obtained by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). With the data set obtained in this study and already published metabolomics data from seed and fruit, we performed QTL mapping, heritability and correlation analyses. Results: Changes in metabolite contents were evident in the ILs that are potentially important with respect to stress responses and plant physiology. By analyzing the obtained data, we identified 42 positive and 76 negative mQTL involved in carbon and nitrogen metabolism. Conclusions: Overall, these findings allowed the identification of S. lycopersicum genome regions involved in the regulation of leaf primary carbon and nitrogen metabolism, as well as the association of leaf metabolites with metabolites from seeds and fruits.
Fil: Nunes Nesi, Adriano. Max Planck Institute Of Molecular Plant Physiology; Alemania. Universidade Federal de Viçosa.; Brasil
Fil: Alseekh, Saleh. Center Of Plant Systems Biology And Biotechnology; Bulgaria. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: de Oliveira Silva, Franklin Magnum. Universidade Federal de Viçosa.; Brasil
Fil: Omranian, Nooshin. Max Planck Institute Of Molecular Plant Physiology; Alemania. Center Of Plant Systems Biology And Biotechnology; Bulgaria
Fil: Lichtenstein, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Mirnezhad, Mohammad. Leiden University; Países Bajos
Fil: Romero González, Roman R.. Leiden University; Países Bajos
Fil: Sabio y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Conte, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Leiss, Kirsten A.. Leiden University; Países Bajos
Fil: Klinkhamer, Peter G. L.. Leiden University; Países Bajos
Fil: Nikoloski, Zoran. University of Potsdam; Alemania. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Fernie, Alisdair R.. Max Planck Institute of Molecular Plant Physiology; Alemania. Center of Plant System Biology and Biotechnology; Bulgaria
Materia
LEAF METABOLISM
METABOLITE NETWORK
METABOLITE QTL
TOMATO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/133396
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/133396
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seedsNunes Nesi, AdrianoAlseekh, Salehde Oliveira Silva, Franklin MagnumOmranian, NooshinLichtenstein, GabrielMirnezhad, MohammadRomero González, Roman R.Sabio y Garcia, Julia VeronicaConte, MarianaLeiss, Kirsten A.Klinkhamer, Peter G. L.Nikoloski, ZoranCarrari, Fernando OscarFernie, Alisdair R.LEAF METABOLISMMETABOLITE NETWORKMETABOLITE QTLTOMATOhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Introduction: To date, most studies of natural variation and metabolite quantitative trait loci (mQTL) in tomato have focused on fruit metabolism, leaving aside the identification of genomic regions involved in the regulation of leaf metabolism. Objective: This study was conducted to identify leaf mQTL in tomato and to assess the association of leaf metabolites and physiological traits with the metabolite levels from other tissues. Methods: The analysis of components of leaf metabolism was performed by phenotypying 76 tomato ILs with chromosome segments of the wild species Solanum pennellii in the genetic background of a cultivated tomato (S. lycopersicum) variety M82. The plants were cultivated in two different environments in independent years and samples were harvested from mature leaves of non-flowering plants at the middle of the light period. The non-targeted metabolite profiling was obtained by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). With the data set obtained in this study and already published metabolomics data from seed and fruit, we performed QTL mapping, heritability and correlation analyses. Results: Changes in metabolite contents were evident in the ILs that are potentially important with respect to stress responses and plant physiology. By analyzing the obtained data, we identified 42 positive and 76 negative mQTL involved in carbon and nitrogen metabolism. Conclusions: Overall, these findings allowed the identification of S. lycopersicum genome regions involved in the regulation of leaf primary carbon and nitrogen metabolism, as well as the association of leaf metabolites with metabolites from seeds and fruits.Fil: Nunes Nesi, Adriano. Max Planck Institute Of Molecular Plant Physiology; Alemania. Universidade Federal de Viçosa.; BrasilFil: Alseekh, Saleh. Center Of Plant Systems Biology And Biotechnology; Bulgaria. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: de Oliveira Silva, Franklin Magnum. Universidade Federal de Viçosa.; BrasilFil: Omranian, Nooshin. Max Planck Institute Of Molecular Plant Physiology; Alemania. Center Of Plant Systems Biology And Biotechnology; BulgariaFil: Lichtenstein, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Mirnezhad, Mohammad. Leiden University; Países BajosFil: Romero González, Roman R.. Leiden University; Países BajosFil: Sabio y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Conte, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Leiss, Kirsten A.. Leiden University; Países BajosFil: Klinkhamer, Peter G. L.. Leiden University; Países BajosFil: Nikoloski, Zoran. University of Potsdam; Alemania. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Fernie, Alisdair R.. Max Planck Institute of Molecular Plant Physiology; Alemania. Center of Plant System Biology and Biotechnology; BulgariaSpringer2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/133396Nunes Nesi, Adriano; Alseekh, Saleh; de Oliveira Silva, Franklin Magnum; Omranian, Nooshin; Lichtenstein, Gabriel; et al.; Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds; Springer; Metabolomics; 15; 4; 4-2019; 1-131573-3882CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11306-019-1503-8info:eu-repo/semantics/altIdentifier/doi/10.1007/s11306-019-1503-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:23Zoai:ri.conicet.gov.ar:11336/133396instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:23.512CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
title Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
spellingShingle Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
Nunes Nesi, Adriano
LEAF METABOLISM
METABOLITE NETWORK
METABOLITE QTL
TOMATO
title_short Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
title_full Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
title_fullStr Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
title_full_unstemmed Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
title_sort Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
dc.creator.none.fl_str_mv Nunes Nesi, Adriano
Alseekh, Saleh
de Oliveira Silva, Franklin Magnum
Omranian, Nooshin
Lichtenstein, Gabriel
Mirnezhad, Mohammad
Romero González, Roman R.
Sabio y Garcia, Julia Veronica
Conte, Mariana
Leiss, Kirsten A.
Klinkhamer, Peter G. L.
Nikoloski, Zoran
Carrari, Fernando Oscar
Fernie, Alisdair R.
author Nunes Nesi, Adriano
author_facet Nunes Nesi, Adriano
Alseekh, Saleh
de Oliveira Silva, Franklin Magnum
Omranian, Nooshin
Lichtenstein, Gabriel
Mirnezhad, Mohammad
Romero González, Roman R.
Sabio y Garcia, Julia Veronica
Conte, Mariana
Leiss, Kirsten A.
Klinkhamer, Peter G. L.
Nikoloski, Zoran
Carrari, Fernando Oscar
Fernie, Alisdair R.
author_role author
author2 Alseekh, Saleh
de Oliveira Silva, Franklin Magnum
Omranian, Nooshin
Lichtenstein, Gabriel
Mirnezhad, Mohammad
Romero González, Roman R.
Sabio y Garcia, Julia Veronica
Conte, Mariana
Leiss, Kirsten A.
Klinkhamer, Peter G. L.
Nikoloski, Zoran
Carrari, Fernando Oscar
Fernie, Alisdair R.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LEAF METABOLISM
METABOLITE NETWORK
METABOLITE QTL
TOMATO
topic LEAF METABOLISM
METABOLITE NETWORK
METABOLITE QTL
TOMATO
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Introduction: To date, most studies of natural variation and metabolite quantitative trait loci (mQTL) in tomato have focused on fruit metabolism, leaving aside the identification of genomic regions involved in the regulation of leaf metabolism. Objective: This study was conducted to identify leaf mQTL in tomato and to assess the association of leaf metabolites and physiological traits with the metabolite levels from other tissues. Methods: The analysis of components of leaf metabolism was performed by phenotypying 76 tomato ILs with chromosome segments of the wild species Solanum pennellii in the genetic background of a cultivated tomato (S. lycopersicum) variety M82. The plants were cultivated in two different environments in independent years and samples were harvested from mature leaves of non-flowering plants at the middle of the light period. The non-targeted metabolite profiling was obtained by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). With the data set obtained in this study and already published metabolomics data from seed and fruit, we performed QTL mapping, heritability and correlation analyses. Results: Changes in metabolite contents were evident in the ILs that are potentially important with respect to stress responses and plant physiology. By analyzing the obtained data, we identified 42 positive and 76 negative mQTL involved in carbon and nitrogen metabolism. Conclusions: Overall, these findings allowed the identification of S. lycopersicum genome regions involved in the regulation of leaf primary carbon and nitrogen metabolism, as well as the association of leaf metabolites with metabolites from seeds and fruits.
Fil: Nunes Nesi, Adriano. Max Planck Institute Of Molecular Plant Physiology; Alemania. Universidade Federal de Viçosa.; Brasil
Fil: Alseekh, Saleh. Center Of Plant Systems Biology And Biotechnology; Bulgaria. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: de Oliveira Silva, Franklin Magnum. Universidade Federal de Viçosa.; Brasil
Fil: Omranian, Nooshin. Max Planck Institute Of Molecular Plant Physiology; Alemania. Center Of Plant Systems Biology And Biotechnology; Bulgaria
Fil: Lichtenstein, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Mirnezhad, Mohammad. Leiden University; Países Bajos
Fil: Romero González, Roman R.. Leiden University; Países Bajos
Fil: Sabio y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Conte, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Leiss, Kirsten A.. Leiden University; Países Bajos
Fil: Klinkhamer, Peter G. L.. Leiden University; Países Bajos
Fil: Nikoloski, Zoran. University of Potsdam; Alemania. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Fernie, Alisdair R.. Max Planck Institute of Molecular Plant Physiology; Alemania. Center of Plant System Biology and Biotechnology; Bulgaria
description Introduction: To date, most studies of natural variation and metabolite quantitative trait loci (mQTL) in tomato have focused on fruit metabolism, leaving aside the identification of genomic regions involved in the regulation of leaf metabolism. Objective: This study was conducted to identify leaf mQTL in tomato and to assess the association of leaf metabolites and physiological traits with the metabolite levels from other tissues. Methods: The analysis of components of leaf metabolism was performed by phenotypying 76 tomato ILs with chromosome segments of the wild species Solanum pennellii in the genetic background of a cultivated tomato (S. lycopersicum) variety M82. The plants were cultivated in two different environments in independent years and samples were harvested from mature leaves of non-flowering plants at the middle of the light period. The non-targeted metabolite profiling was obtained by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). With the data set obtained in this study and already published metabolomics data from seed and fruit, we performed QTL mapping, heritability and correlation analyses. Results: Changes in metabolite contents were evident in the ILs that are potentially important with respect to stress responses and plant physiology. By analyzing the obtained data, we identified 42 positive and 76 negative mQTL involved in carbon and nitrogen metabolism. Conclusions: Overall, these findings allowed the identification of S. lycopersicum genome regions involved in the regulation of leaf primary carbon and nitrogen metabolism, as well as the association of leaf metabolites with metabolites from seeds and fruits.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/133396
Nunes Nesi, Adriano; Alseekh, Saleh; de Oliveira Silva, Franklin Magnum; Omranian, Nooshin; Lichtenstein, Gabriel; et al.; Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds; Springer; Metabolomics; 15; 4; 4-2019; 1-13
1573-3882
CONICET Digital
CONICET
url http://hdl.handle.net/11336/133396
identifier_str_mv Nunes Nesi, Adriano; Alseekh, Saleh; de Oliveira Silva, Franklin Magnum; Omranian, Nooshin; Lichtenstein, Gabriel; et al.; Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds; Springer; Metabolomics; 15; 4; 4-2019; 1-13
1573-3882
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11306-019-1503-8
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11306-019-1503-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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