A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata)

Autores
Gonzalez Roca, Lucia; Iannicelli, Jesica; Coviella, Maria Andrea; Bugallo, Veronica Lucia; Bologna, Paula; Pitta Alvarez, Sandra Irene; Escandon, Alejandro Salvio
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The genus Glandularia (Verbenaceae) has more than 50 species and it holds great ornamental potential due to its colorful flowers, long flowering period and low water requirements. Since the floriculture market avidly seeks novelties, increasing the diversity within the genus would increase its commercial value. Some of the traits that should be improved in these species are their architecture and the size and colors of the flowers and leaves. In this sense, obtaining polyploid individuals is an interesting strategy to achieve this objective. In this paper a hybrid of Glandularia peruviana × Glandularia scrobiculata was cultured under in vitro conditions. Different plant growth regulators and their combinations were tested to obtain appropriate multiplication rates. The best results were obtained with the combination of 6.6 μM thidiazuron/0.03 μM α-naphthalenacetic acid, with a multiplication rate of 19 shoots per explant. The plantlets were first rooted and then acclimatized by transferal to an 8.0 cm diameter pot containing Growing mix®, and maintenance inside a humidity chamber. For poliploydization, the explants were exposed to colchicine in concentrations of 0.001% and 0.01% for 24 and 48 h. The 40 recovered plants were characterized according to their DNA content. There were 21 diploids, 14 solid tetraploids, 1 solid octoploid, 3 chimera tetraploids and 1 chimera octoploid. Phenotypically, the size of the flowers, inflorescences, pollen grains and stomata were significantly larger in polyploid individuals. Surprisingly, stem diameter, leaf size and color intensity of the leaves and flowers were not significantly different between diploid and tetraploid individuals. Due to the size of the inflorescences, the tetraploid individuals are a promising starting material for a Glandularia breeding program.
Instituto de Floricultura
Fil: Gonzalez Roca, Lucia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Iannicelli, Jesica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Coviella, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Bugallo, Verónica. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Genética; Argentina
Fil: Bologna, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Pitta Alvarez, Sandra Irene. Universidad Argentina de la Empresa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Biotecnología Algal y Vegetal; Argentina
Fil: Escandon, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fuente
Scientia Horticulturae 184 : 46-54 (March 2015)
Materia
Verbenaceae
Experimentación in Vitro
Poliploidia Inducida
Propagación Vegetativa
Hibridación Interespecífica
In Vitro Experimentation
Induced Polyploidy
Vegetative Propagation
Interspecific Hybridization
Glandularia
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2883
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2883
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata)Gonzalez Roca, LuciaIannicelli, JesicaCoviella, Maria AndreaBugallo, Veronica LuciaBologna, PaulaPitta Alvarez, Sandra IreneEscandon, Alejandro SalvioVerbenaceaeExperimentación in VitroPoliploidia InducidaPropagación VegetativaHibridación InterespecíficaIn Vitro ExperimentationInduced PolyploidyVegetative PropagationInterspecific HybridizationGlandulariaThe genus Glandularia (Verbenaceae) has more than 50 species and it holds great ornamental potential due to its colorful flowers, long flowering period and low water requirements. Since the floriculture market avidly seeks novelties, increasing the diversity within the genus would increase its commercial value. Some of the traits that should be improved in these species are their architecture and the size and colors of the flowers and leaves. In this sense, obtaining polyploid individuals is an interesting strategy to achieve this objective. In this paper a hybrid of Glandularia peruviana × Glandularia scrobiculata was cultured under in vitro conditions. Different plant growth regulators and their combinations were tested to obtain appropriate multiplication rates. The best results were obtained with the combination of 6.6 μM thidiazuron/0.03 μM α-naphthalenacetic acid, with a multiplication rate of 19 shoots per explant. The plantlets were first rooted and then acclimatized by transferal to an 8.0 cm diameter pot containing Growing mix®, and maintenance inside a humidity chamber. For poliploydization, the explants were exposed to colchicine in concentrations of 0.001% and 0.01% for 24 and 48 h. The 40 recovered plants were characterized according to their DNA content. There were 21 diploids, 14 solid tetraploids, 1 solid octoploid, 3 chimera tetraploids and 1 chimera octoploid. Phenotypically, the size of the flowers, inflorescences, pollen grains and stomata were significantly larger in polyploid individuals. Surprisingly, stem diameter, leaf size and color intensity of the leaves and flowers were not significantly different between diploid and tetraploid individuals. Due to the size of the inflorescences, the tetraploid individuals are a promising starting material for a Glandularia breeding program.Instituto de FloriculturaFil: Gonzalez Roca, Lucia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; ArgentinaFil: Iannicelli, Jesica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Coviella, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; ArgentinaFil: Bugallo, Verónica. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Genética; ArgentinaFil: Bologna, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; ArgentinaFil: Pitta Alvarez, Sandra Irene. Universidad Argentina de la Empresa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Biotecnología Algal y Vegetal; ArgentinaFil: Escandon, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina2018-07-26T12:55:08Z2018-07-26T12:55:08Z2015-03-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2883https://www.sciencedirect.com/science/article/pii/S03044238140070550304-4238https://doi.org/10.1016/j.scienta.2014.12.032Scientia Horticulturae 184 : 46-54 (March 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:22Zoai:localhost:20.500.12123/2883instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:23.157INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata)
title A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata)
spellingShingle A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata)
Gonzalez Roca, Lucia
Verbenaceae
Experimentación in Vitro
Poliploidia Inducida
Propagación Vegetativa
Hibridación Interespecífica
In Vitro Experimentation
Induced Polyploidy
Vegetative Propagation
Interspecific Hybridization
Glandularia
title_short A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata)
title_full A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata)
title_fullStr A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata)
title_full_unstemmed A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata)
title_sort A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana × G. scrobiculata)
dc.creator.none.fl_str_mv Gonzalez Roca, Lucia
Iannicelli, Jesica
Coviella, Maria Andrea
Bugallo, Veronica Lucia
Bologna, Paula
Pitta Alvarez, Sandra Irene
Escandon, Alejandro Salvio
author Gonzalez Roca, Lucia
author_facet Gonzalez Roca, Lucia
Iannicelli, Jesica
Coviella, Maria Andrea
Bugallo, Veronica Lucia
Bologna, Paula
Pitta Alvarez, Sandra Irene
Escandon, Alejandro Salvio
author_role author
author2 Iannicelli, Jesica
Coviella, Maria Andrea
Bugallo, Veronica Lucia
Bologna, Paula
Pitta Alvarez, Sandra Irene
Escandon, Alejandro Salvio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Verbenaceae
Experimentación in Vitro
Poliploidia Inducida
Propagación Vegetativa
Hibridación Interespecífica
In Vitro Experimentation
Induced Polyploidy
Vegetative Propagation
Interspecific Hybridization
Glandularia
topic Verbenaceae
Experimentación in Vitro
Poliploidia Inducida
Propagación Vegetativa
Hibridación Interespecífica
In Vitro Experimentation
Induced Polyploidy
Vegetative Propagation
Interspecific Hybridization
Glandularia
dc.description.none.fl_txt_mv The genus Glandularia (Verbenaceae) has more than 50 species and it holds great ornamental potential due to its colorful flowers, long flowering period and low water requirements. Since the floriculture market avidly seeks novelties, increasing the diversity within the genus would increase its commercial value. Some of the traits that should be improved in these species are their architecture and the size and colors of the flowers and leaves. In this sense, obtaining polyploid individuals is an interesting strategy to achieve this objective. In this paper a hybrid of Glandularia peruviana × Glandularia scrobiculata was cultured under in vitro conditions. Different plant growth regulators and their combinations were tested to obtain appropriate multiplication rates. The best results were obtained with the combination of 6.6 μM thidiazuron/0.03 μM α-naphthalenacetic acid, with a multiplication rate of 19 shoots per explant. The plantlets were first rooted and then acclimatized by transferal to an 8.0 cm diameter pot containing Growing mix®, and maintenance inside a humidity chamber. For poliploydization, the explants were exposed to colchicine in concentrations of 0.001% and 0.01% for 24 and 48 h. The 40 recovered plants were characterized according to their DNA content. There were 21 diploids, 14 solid tetraploids, 1 solid octoploid, 3 chimera tetraploids and 1 chimera octoploid. Phenotypically, the size of the flowers, inflorescences, pollen grains and stomata were significantly larger in polyploid individuals. Surprisingly, stem diameter, leaf size and color intensity of the leaves and flowers were not significantly different between diploid and tetraploid individuals. Due to the size of the inflorescences, the tetraploid individuals are a promising starting material for a Glandularia breeding program.
Instituto de Floricultura
Fil: Gonzalez Roca, Lucia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Iannicelli, Jesica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Coviella, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Bugallo, Verónica. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Genética; Argentina
Fil: Bologna, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Pitta Alvarez, Sandra Irene. Universidad Argentina de la Empresa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Biotecnología Algal y Vegetal; Argentina
Fil: Escandon, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
description The genus Glandularia (Verbenaceae) has more than 50 species and it holds great ornamental potential due to its colorful flowers, long flowering period and low water requirements. Since the floriculture market avidly seeks novelties, increasing the diversity within the genus would increase its commercial value. Some of the traits that should be improved in these species are their architecture and the size and colors of the flowers and leaves. In this sense, obtaining polyploid individuals is an interesting strategy to achieve this objective. In this paper a hybrid of Glandularia peruviana × Glandularia scrobiculata was cultured under in vitro conditions. Different plant growth regulators and their combinations were tested to obtain appropriate multiplication rates. The best results were obtained with the combination of 6.6 μM thidiazuron/0.03 μM α-naphthalenacetic acid, with a multiplication rate of 19 shoots per explant. The plantlets were first rooted and then acclimatized by transferal to an 8.0 cm diameter pot containing Growing mix®, and maintenance inside a humidity chamber. For poliploydization, the explants were exposed to colchicine in concentrations of 0.001% and 0.01% for 24 and 48 h. The 40 recovered plants were characterized according to their DNA content. There were 21 diploids, 14 solid tetraploids, 1 solid octoploid, 3 chimera tetraploids and 1 chimera octoploid. Phenotypically, the size of the flowers, inflorescences, pollen grains and stomata were significantly larger in polyploid individuals. Surprisingly, stem diameter, leaf size and color intensity of the leaves and flowers were not significantly different between diploid and tetraploid individuals. Due to the size of the inflorescences, the tetraploid individuals are a promising starting material for a Glandularia breeding program.
publishDate 2015
dc.date.none.fl_str_mv 2015-03-05
2018-07-26T12:55:08Z
2018-07-26T12:55:08Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2883
https://www.sciencedirect.com/science/article/pii/S0304423814007055
0304-4238
https://doi.org/10.1016/j.scienta.2014.12.032
url http://hdl.handle.net/20.500.12123/2883
https://www.sciencedirect.com/science/article/pii/S0304423814007055
https://doi.org/10.1016/j.scienta.2014.12.032
identifier_str_mv 0304-4238
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scientia Horticulturae 184 : 46-54 (March 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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