A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana×G. scrobiculata)

Autores
González Roca, Lucía; Iannicelli, Jesica; Coviella, Andrea; Bugallo, Verónica; Bologna, Paula; Pitta Alvarez, Sandra Irene; Escandón, Alejandro Salvio
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The genus Glandularia (Verbenaceae) has more than 50 species and it holds great ornamental potential due to its colorful flowers, long flowering period and low water requirements. Since the floriculture market avidly seeks novelties, increasing the diversity within the genus would increase its commercial value. Some of the traits that should be improved in these species are their architecture and the size and colors of the flowers and leaves. In this sense, obtaining polyploid individuals is an interesting strategy to achieve this objective. In this paper a hybrid of Glandularia peruviana×Glandularia scrobiculata was cultured under in vitro conditions. Different plant growth regulators and their combinations were tested to obtain appropriate multiplication rates. The best results were obtained with the combination of 6.6μM thidiazuron/0.03μM α-naphthalenacetic acid, with a multiplication rate of 19 shoots per explant. The plantlets were first rooted and then acclimatized by transferal to an 8.0cm diameter pot containing Growing mix®, and maintenance inside a humidity chamber. For poliploydization, the explants were exposed to colchicine in concentrations of 0.001% and 0.01% for 24 and 48h. The 40 recovered plants were characterized according to their DNA content. There were 21 diploids, 14 solid tetraploids, 1 solid octoploid, 3 chimera tetraploids and 1 chimera octoploid. Phenotypically, the size of the flowers, inflorescences, pollen grains and stomata were significantly larger in polyploid individuals. Surprisingly, stem diameter, leaf size and color intensity of the leaves and flowers were not significantly different between diploid and tetraploid individuals. Due to the size of the inflorescences, the tetraploid individuals are a promising starting material for a Glandularia breeding program.
Fil: González Roca, Lucía. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
Fil: Iannicelli, Jesica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Coviella, Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
Fil: Bugallo, Verónica. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Bologna, Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
Fil: Pitta Alvarez, Sandra Irene. Universidad Argentina de la Empresa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escandón, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Materia
Breeding
Ornamental Plants
Plant Biotechnology
Tissue Culture
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37698
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/37698
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana×G. scrobiculata)González Roca, LucíaIannicelli, JesicaCoviella, AndreaBugallo, VerónicaBologna, PaulaPitta Alvarez, Sandra IreneEscandón, Alejandro SalvioBreedingOrnamental PlantsPlant BiotechnologyTissue Culturehttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The genus Glandularia (Verbenaceae) has more than 50 species and it holds great ornamental potential due to its colorful flowers, long flowering period and low water requirements. Since the floriculture market avidly seeks novelties, increasing the diversity within the genus would increase its commercial value. Some of the traits that should be improved in these species are their architecture and the size and colors of the flowers and leaves. In this sense, obtaining polyploid individuals is an interesting strategy to achieve this objective. In this paper a hybrid of Glandularia peruviana×Glandularia scrobiculata was cultured under in vitro conditions. Different plant growth regulators and their combinations were tested to obtain appropriate multiplication rates. The best results were obtained with the combination of 6.6μM thidiazuron/0.03μM α-naphthalenacetic acid, with a multiplication rate of 19 shoots per explant. The plantlets were first rooted and then acclimatized by transferal to an 8.0cm diameter pot containing Growing mix®, and maintenance inside a humidity chamber. For poliploydization, the explants were exposed to colchicine in concentrations of 0.001% and 0.01% for 24 and 48h. The 40 recovered plants were characterized according to their DNA content. There were 21 diploids, 14 solid tetraploids, 1 solid octoploid, 3 chimera tetraploids and 1 chimera octoploid. Phenotypically, the size of the flowers, inflorescences, pollen grains and stomata were significantly larger in polyploid individuals. Surprisingly, stem diameter, leaf size and color intensity of the leaves and flowers were not significantly different between diploid and tetraploid individuals. Due to the size of the inflorescences, the tetraploid individuals are a promising starting material for a Glandularia breeding program.Fil: González Roca, Lucía. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; ArgentinaFil: Iannicelli, Jesica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Coviella, Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; ArgentinaFil: Bugallo, Verónica. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Bologna, Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; ArgentinaFil: Pitta Alvarez, Sandra Irene. Universidad Argentina de la Empresa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Escandón, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaElsevier Science2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37698González Roca, Lucía; Iannicelli, Jesica; Coviella, Andrea; Bugallo, Verónica; Bologna, Paula; et al.; A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana×G. scrobiculata); Elsevier Science; Scientia Horticulturae; 184; 3-2015; 46-540304-4238CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2014.12.032info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304423814007055info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:37Zoai:ri.conicet.gov.ar:11336/37698instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:37.588CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana×G. scrobiculata)
title A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana×G. scrobiculata)
spellingShingle A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana×G. scrobiculata)
González Roca, Lucía
Breeding
Ornamental Plants
Plant Biotechnology
Tissue Culture
title_short A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana×G. scrobiculata)
title_full A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana×G. scrobiculata)
title_fullStr A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana×G. scrobiculata)
title_full_unstemmed A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana×G. scrobiculata)
title_sort A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana×G. scrobiculata)
dc.creator.none.fl_str_mv González Roca, Lucía
Iannicelli, Jesica
Coviella, Andrea
Bugallo, Verónica
Bologna, Paula
Pitta Alvarez, Sandra Irene
Escandón, Alejandro Salvio
author González Roca, Lucía
author_facet González Roca, Lucía
Iannicelli, Jesica
Coviella, Andrea
Bugallo, Verónica
Bologna, Paula
Pitta Alvarez, Sandra Irene
Escandón, Alejandro Salvio
author_role author
author2 Iannicelli, Jesica
Coviella, Andrea
Bugallo, Verónica
Bologna, Paula
Pitta Alvarez, Sandra Irene
Escandón, Alejandro Salvio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Breeding
Ornamental Plants
Plant Biotechnology
Tissue Culture
topic Breeding
Ornamental Plants
Plant Biotechnology
Tissue Culture
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The genus Glandularia (Verbenaceae) has more than 50 species and it holds great ornamental potential due to its colorful flowers, long flowering period and low water requirements. Since the floriculture market avidly seeks novelties, increasing the diversity within the genus would increase its commercial value. Some of the traits that should be improved in these species are their architecture and the size and colors of the flowers and leaves. In this sense, obtaining polyploid individuals is an interesting strategy to achieve this objective. In this paper a hybrid of Glandularia peruviana×Glandularia scrobiculata was cultured under in vitro conditions. Different plant growth regulators and their combinations were tested to obtain appropriate multiplication rates. The best results were obtained with the combination of 6.6μM thidiazuron/0.03μM α-naphthalenacetic acid, with a multiplication rate of 19 shoots per explant. The plantlets were first rooted and then acclimatized by transferal to an 8.0cm diameter pot containing Growing mix®, and maintenance inside a humidity chamber. For poliploydization, the explants were exposed to colchicine in concentrations of 0.001% and 0.01% for 24 and 48h. The 40 recovered plants were characterized according to their DNA content. There were 21 diploids, 14 solid tetraploids, 1 solid octoploid, 3 chimera tetraploids and 1 chimera octoploid. Phenotypically, the size of the flowers, inflorescences, pollen grains and stomata were significantly larger in polyploid individuals. Surprisingly, stem diameter, leaf size and color intensity of the leaves and flowers were not significantly different between diploid and tetraploid individuals. Due to the size of the inflorescences, the tetraploid individuals are a promising starting material for a Glandularia breeding program.
Fil: González Roca, Lucía. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
Fil: Iannicelli, Jesica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Coviella, Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
Fil: Bugallo, Verónica. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Bologna, Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
Fil: Pitta Alvarez, Sandra Irene. Universidad Argentina de la Empresa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escandón, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
description The genus Glandularia (Verbenaceae) has more than 50 species and it holds great ornamental potential due to its colorful flowers, long flowering period and low water requirements. Since the floriculture market avidly seeks novelties, increasing the diversity within the genus would increase its commercial value. Some of the traits that should be improved in these species are their architecture and the size and colors of the flowers and leaves. In this sense, obtaining polyploid individuals is an interesting strategy to achieve this objective. In this paper a hybrid of Glandularia peruviana×Glandularia scrobiculata was cultured under in vitro conditions. Different plant growth regulators and their combinations were tested to obtain appropriate multiplication rates. The best results were obtained with the combination of 6.6μM thidiazuron/0.03μM α-naphthalenacetic acid, with a multiplication rate of 19 shoots per explant. The plantlets were first rooted and then acclimatized by transferal to an 8.0cm diameter pot containing Growing mix®, and maintenance inside a humidity chamber. For poliploydization, the explants were exposed to colchicine in concentrations of 0.001% and 0.01% for 24 and 48h. The 40 recovered plants were characterized according to their DNA content. There were 21 diploids, 14 solid tetraploids, 1 solid octoploid, 3 chimera tetraploids and 1 chimera octoploid. Phenotypically, the size of the flowers, inflorescences, pollen grains and stomata were significantly larger in polyploid individuals. Surprisingly, stem diameter, leaf size and color intensity of the leaves and flowers were not significantly different between diploid and tetraploid individuals. Due to the size of the inflorescences, the tetraploid individuals are a promising starting material for a Glandularia breeding program.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37698
González Roca, Lucía; Iannicelli, Jesica; Coviella, Andrea; Bugallo, Verónica; Bologna, Paula; et al.; A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana×G. scrobiculata); Elsevier Science; Scientia Horticulturae; 184; 3-2015; 46-54
0304-4238
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37698
identifier_str_mv González Roca, Lucía; Iannicelli, Jesica; Coviella, Andrea; Bugallo, Verónica; Bologna, Paula; et al.; A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana×G. scrobiculata); Elsevier Science; Scientia Horticulturae; 184; 3-2015; 46-54
0304-4238
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2014.12.032
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304423814007055
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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