In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus.

Autores
Di Ciaccio, Lucía Soledad; Catalano, Alejandro V.; López, Paula G.; Rojas, Dante Emanuel; Cristos, Diego Sebastian; Fortunato, Renee Hersilia; Salvat, Adriana Elisabeth
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aspergillus flavus is a filamentous, saprophytic fungus, whose colonization occurs mainly in cereal grains and oilseeds once arvested. Under certain conditions, it could produce mycotoxins called aflatoxins, known as powerful human liver carcinogens. The aim of the present study was to describe the antifungal activity of extracts of Peltophorum dubium, a species from northern Argentina (Oriental Chaco), against A. flavus. The antifungal activities of di erent collection sites are reported. The extracts exhibited a minimum inhibitory concentration of 125 g/mL, and the di erences between the treatments and the inoculum control were 11 mm of P. dubium A and 10 mm of P. dubium F in colony growth. Moreover, hyphae treated with the extracts stained blue with Evans blue showed alterations in the membrane and/or cell wall, allowing the dye income. Bio-guided fractionation, High Performance Liquid Chromatography diode array ultraviolet/visible (HPLC UV/VIS DAD), and Ultra-High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry (UPLC ESI-MS) analyses were conducted to characterize the extracts and their active fractions. The HPLC UV/VIS DAD analysis allowed the determination of the presence of flavonoids (flavonols and flavones), coumarins, terpenes, and steroids. UPLC ESI/MS analysis of active fractions revealed the presence of Kaempferol, Apigenin, Naringenin, Chrysin and aidzein.
Fil: Di Ciaccio, Lucí­a Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Catalano, Alejandra V. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Farmacognosia; Argentina
Fil: López, Paula G. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Farmacognosia; Argentina
Fil: Rojas, Dante. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina
Fil: Fortunato, Renee Hersilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Salvat, Adriana Elisabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Cristos, Diego Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina
Fuente
Plants 9 (4) : 438 (2020)
Materia
Aspergillus flavus
Flavonoides
Flavonoids
Plant Bioactivity
Evans Blue
Active Extract Characterization
Bioactividad de la Planta
Evans Azul
Caracterización de Extracto Activo
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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oai_identifier_str oai:localhost:20.500.12123/7283
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spelling In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus.Di Ciaccio, Lucía SoledadCatalano, Alejandro V.López, Paula G.Rojas, Dante EmanuelCristos, Diego SebastianFortunato, Renee HersiliaSalvat, Adriana ElisabethAspergillus flavusFlavonoidesFlavonoidsPlant BioactivityEvans BlueActive Extract CharacterizationBioactividad de la PlantaEvans AzulCaracterización de Extracto ActivoAspergillus flavus is a filamentous, saprophytic fungus, whose colonization occurs mainly in cereal grains and oilseeds once arvested. Under certain conditions, it could produce mycotoxins called aflatoxins, known as powerful human liver carcinogens. The aim of the present study was to describe the antifungal activity of extracts of Peltophorum dubium, a species from northern Argentina (Oriental Chaco), against A. flavus. The antifungal activities of di erent collection sites are reported. The extracts exhibited a minimum inhibitory concentration of 125 g/mL, and the di erences between the treatments and the inoculum control were 11 mm of P. dubium A and 10 mm of P. dubium F in colony growth. Moreover, hyphae treated with the extracts stained blue with Evans blue showed alterations in the membrane and/or cell wall, allowing the dye income. Bio-guided fractionation, High Performance Liquid Chromatography diode array ultraviolet/visible (HPLC UV/VIS DAD), and Ultra-High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry (UPLC ESI-MS) analyses were conducted to characterize the extracts and their active fractions. The HPLC UV/VIS DAD analysis allowed the determination of the presence of flavonoids (flavonols and flavones), coumarins, terpenes, and steroids. UPLC ESI/MS analysis of active fractions revealed the presence of Kaempferol, Apigenin, Naringenin, Chrysin and aidzein.Fil: Di Ciaccio, Lucí­a Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Catalano, Alejandra V. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Farmacognosia; ArgentinaFil: López, Paula G. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Farmacognosia; ArgentinaFil: Rojas, Dante. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; ArgentinaFil: Fortunato, Renee Hersilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Salvat, Adriana Elisabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Cristos, Diego Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; ArgentinaMDPI2020-05-21T11:37:33Z2020-05-21T11:37:33Z2020-04-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7283https://www.mdpi.com/2223-747/9/4/4382223-7747https://doi.org/10.3390/plants9040438Plants 9 (4) : 438 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:56Zoai:localhost:20.500.12123/7283instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:57.018INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus.
title In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus.
spellingShingle In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus.
Di Ciaccio, Lucía Soledad
Aspergillus flavus
Flavonoides
Flavonoids
Plant Bioactivity
Evans Blue
Active Extract Characterization
Bioactividad de la Planta
Evans Azul
Caracterización de Extracto Activo
title_short In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus.
title_full In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus.
title_fullStr In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus.
title_full_unstemmed In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus.
title_sort In vitro antifungal activity of Peltophorum dubium (Spreg.) Taub. extracts against Aspergillus flavus.
dc.creator.none.fl_str_mv Di Ciaccio, Lucía Soledad
Catalano, Alejandro V.
López, Paula G.
Rojas, Dante Emanuel
Cristos, Diego Sebastian
Fortunato, Renee Hersilia
Salvat, Adriana Elisabeth
author Di Ciaccio, Lucía Soledad
author_facet Di Ciaccio, Lucía Soledad
Catalano, Alejandro V.
López, Paula G.
Rojas, Dante Emanuel
Cristos, Diego Sebastian
Fortunato, Renee Hersilia
Salvat, Adriana Elisabeth
author_role author
author2 Catalano, Alejandro V.
López, Paula G.
Rojas, Dante Emanuel
Cristos, Diego Sebastian
Fortunato, Renee Hersilia
Salvat, Adriana Elisabeth
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Aspergillus flavus
Flavonoides
Flavonoids
Plant Bioactivity
Evans Blue
Active Extract Characterization
Bioactividad de la Planta
Evans Azul
Caracterización de Extracto Activo
topic Aspergillus flavus
Flavonoides
Flavonoids
Plant Bioactivity
Evans Blue
Active Extract Characterization
Bioactividad de la Planta
Evans Azul
Caracterización de Extracto Activo
dc.description.none.fl_txt_mv Aspergillus flavus is a filamentous, saprophytic fungus, whose colonization occurs mainly in cereal grains and oilseeds once arvested. Under certain conditions, it could produce mycotoxins called aflatoxins, known as powerful human liver carcinogens. The aim of the present study was to describe the antifungal activity of extracts of Peltophorum dubium, a species from northern Argentina (Oriental Chaco), against A. flavus. The antifungal activities of di erent collection sites are reported. The extracts exhibited a minimum inhibitory concentration of 125 g/mL, and the di erences between the treatments and the inoculum control were 11 mm of P. dubium A and 10 mm of P. dubium F in colony growth. Moreover, hyphae treated with the extracts stained blue with Evans blue showed alterations in the membrane and/or cell wall, allowing the dye income. Bio-guided fractionation, High Performance Liquid Chromatography diode array ultraviolet/visible (HPLC UV/VIS DAD), and Ultra-High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry (UPLC ESI-MS) analyses were conducted to characterize the extracts and their active fractions. The HPLC UV/VIS DAD analysis allowed the determination of the presence of flavonoids (flavonols and flavones), coumarins, terpenes, and steroids. UPLC ESI/MS analysis of active fractions revealed the presence of Kaempferol, Apigenin, Naringenin, Chrysin and aidzein.
Fil: Di Ciaccio, Lucí­a Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Catalano, Alejandra V. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Farmacognosia; Argentina
Fil: López, Paula G. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Farmacognosia; Argentina
Fil: Rojas, Dante. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina
Fil: Fortunato, Renee Hersilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Salvat, Adriana Elisabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Cristos, Diego Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina
description Aspergillus flavus is a filamentous, saprophytic fungus, whose colonization occurs mainly in cereal grains and oilseeds once arvested. Under certain conditions, it could produce mycotoxins called aflatoxins, known as powerful human liver carcinogens. The aim of the present study was to describe the antifungal activity of extracts of Peltophorum dubium, a species from northern Argentina (Oriental Chaco), against A. flavus. The antifungal activities of di erent collection sites are reported. The extracts exhibited a minimum inhibitory concentration of 125 g/mL, and the di erences between the treatments and the inoculum control were 11 mm of P. dubium A and 10 mm of P. dubium F in colony growth. Moreover, hyphae treated with the extracts stained blue with Evans blue showed alterations in the membrane and/or cell wall, allowing the dye income. Bio-guided fractionation, High Performance Liquid Chromatography diode array ultraviolet/visible (HPLC UV/VIS DAD), and Ultra-High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry (UPLC ESI-MS) analyses were conducted to characterize the extracts and their active fractions. The HPLC UV/VIS DAD analysis allowed the determination of the presence of flavonoids (flavonols and flavones), coumarins, terpenes, and steroids. UPLC ESI/MS analysis of active fractions revealed the presence of Kaempferol, Apigenin, Naringenin, Chrysin and aidzein.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-21T11:37:33Z
2020-05-21T11:37:33Z
2020-04-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7283
https://www.mdpi.com/2223-747/9/4/438
2223-7747
https://doi.org/10.3390/plants9040438
url http://hdl.handle.net/20.500.12123/7283
https://www.mdpi.com/2223-747/9/4/438
https://doi.org/10.3390/plants9040438
identifier_str_mv 2223-7747
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Plants 9 (4) : 438 (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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