Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteria

Autores
Blajman, Jesica; Vinderola, Celso Gabriel; Cuatrin, Alejandra; Lingua, Mariana Soledad; Paez, Roxana Beatriz
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Aims: To investigate the influence of different culture media and fermentation conditions on growth performance and viability of three lactic acid bacteria (LAB) strains with potential as silage inoculants, and to optimize spray‐drying in order to enhance survival to dehydration and storage stability. Methods and Results: In house‐formulated MRS was a suitable low‐cost culture medium for Lactobacillus plantarum Hv75, Pediococcus acidilactici 3903 and L. buchneri B463. Uncontrolled pH biomass production conferred enhanced stability during storage at 4 °C after spray drying. The use of whey protein concentrate 35 (WPC)‐maltodextrin (M) as matrix, inlet temperature of 145‐150 °C and air flow rate of 601 l h−1 was adequate for the production of dehydrated LAB. According to the desirability function, at this optimized condition, moisture content, yield and solubility were predicted to be 3.96%, 73.68%, and 90.36% respectively. Those conditions also showed a decrease of 0.855 log CFU g‐1 after drying, no loss in viability at 4 °C for 6 months and 1 log CFU g‐1 reduction at 25 °C. Conclusions: Stable and economically feasible dehydrated LAB cultures were obtained by using alternative culture media, fermentation under uncontrolled pH, and optimizing spray‐drying process conditions through the desirability function method.
EEA Rafaela
Fil: Blajman, Jesica E. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina
Fil: Cuatrin, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Lingua, Mariana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina
Fil: Paez, Roxana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fuente
Journal of Applied Microbiology (First published:16 June 2020)
Materia
Ensilado
Bacterias Acidolácticas
Medio de Cultivo
Fermentación
Inoculación
Secado por Pulverización
Silage
Lactic Acid Bacteria
Culture Media
Fermentation
Inoculation
Spray Drying
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7473
Acceder
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oai_identifier_str oai:localhost:20.500.12123/7473
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network_name_str INTA Digital (INTA)
spelling Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteriaBlajman, JesicaVinderola, Celso GabrielCuatrin, AlejandraLingua, Mariana SoledadPaez, Roxana BeatrizEnsiladoBacterias AcidolácticasMedio de CultivoFermentaciónInoculaciónSecado por PulverizaciónSilageLactic Acid BacteriaCulture MediaFermentationInoculationSpray DryingAims: To investigate the influence of different culture media and fermentation conditions on growth performance and viability of three lactic acid bacteria (LAB) strains with potential as silage inoculants, and to optimize spray‐drying in order to enhance survival to dehydration and storage stability. Methods and Results: In house‐formulated MRS was a suitable low‐cost culture medium for Lactobacillus plantarum Hv75, Pediococcus acidilactici 3903 and L. buchneri B463. Uncontrolled pH biomass production conferred enhanced stability during storage at 4 °C after spray drying. The use of whey protein concentrate 35 (WPC)‐maltodextrin (M) as matrix, inlet temperature of 145‐150 °C and air flow rate of 601 l h−1 was adequate for the production of dehydrated LAB. According to the desirability function, at this optimized condition, moisture content, yield and solubility were predicted to be 3.96%, 73.68%, and 90.36% respectively. Those conditions also showed a decrease of 0.855 log CFU g‐1 after drying, no loss in viability at 4 °C for 6 months and 1 log CFU g‐1 reduction at 25 °C. Conclusions: Stable and economically feasible dehydrated LAB cultures were obtained by using alternative culture media, fermentation under uncontrolled pH, and optimizing spray‐drying process conditions through the desirability function method.EEA RafaelaFil: Blajman, Jesica E. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; ArgentinaFil: Cuatrin, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Lingua, Mariana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; ArgentinaFil: Paez, Roxana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaWileyinfo:eu-repo/date/embargoEnd/2021-06-252020-06-25T17:57:54Z2020-06-25T17:57:54Z2020-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7473https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/jam.147501364-50721365-2672https://doi.org/10.1111/jam.14750Journal of Applied Microbiology (First published:16 June 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:28Zoai:localhost:20.500.12123/7473instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:30.322INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteria
title Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteria
spellingShingle Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteria
Blajman, Jesica
Ensilado
Bacterias Acidolácticas
Medio de Cultivo
Fermentación
Inoculación
Secado por Pulverización
Silage
Lactic Acid Bacteria
Culture Media
Fermentation
Inoculation
Spray Drying
title_short Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteria
title_full Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteria
title_fullStr Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteria
title_full_unstemmed Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteria
title_sort Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteria
dc.creator.none.fl_str_mv Blajman, Jesica
Vinderola, Celso Gabriel
Cuatrin, Alejandra
Lingua, Mariana Soledad
Paez, Roxana Beatriz
author Blajman, Jesica
author_facet Blajman, Jesica
Vinderola, Celso Gabriel
Cuatrin, Alejandra
Lingua, Mariana Soledad
Paez, Roxana Beatriz
author_role author
author2 Vinderola, Celso Gabriel
Cuatrin, Alejandra
Lingua, Mariana Soledad
Paez, Roxana Beatriz
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ensilado
Bacterias Acidolácticas
Medio de Cultivo
Fermentación
Inoculación
Secado por Pulverización
Silage
Lactic Acid Bacteria
Culture Media
Fermentation
Inoculation
Spray Drying
topic Ensilado
Bacterias Acidolácticas
Medio de Cultivo
Fermentación
Inoculación
Secado por Pulverización
Silage
Lactic Acid Bacteria
Culture Media
Fermentation
Inoculation
Spray Drying
dc.description.none.fl_txt_mv Aims: To investigate the influence of different culture media and fermentation conditions on growth performance and viability of three lactic acid bacteria (LAB) strains with potential as silage inoculants, and to optimize spray‐drying in order to enhance survival to dehydration and storage stability. Methods and Results: In house‐formulated MRS was a suitable low‐cost culture medium for Lactobacillus plantarum Hv75, Pediococcus acidilactici 3903 and L. buchneri B463. Uncontrolled pH biomass production conferred enhanced stability during storage at 4 °C after spray drying. The use of whey protein concentrate 35 (WPC)‐maltodextrin (M) as matrix, inlet temperature of 145‐150 °C and air flow rate of 601 l h−1 was adequate for the production of dehydrated LAB. According to the desirability function, at this optimized condition, moisture content, yield and solubility were predicted to be 3.96%, 73.68%, and 90.36% respectively. Those conditions also showed a decrease of 0.855 log CFU g‐1 after drying, no loss in viability at 4 °C for 6 months and 1 log CFU g‐1 reduction at 25 °C. Conclusions: Stable and economically feasible dehydrated LAB cultures were obtained by using alternative culture media, fermentation under uncontrolled pH, and optimizing spray‐drying process conditions through the desirability function method.
EEA Rafaela
Fil: Blajman, Jesica E. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina
Fil: Cuatrin, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Lingua, Mariana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina
Fil: Paez, Roxana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
description Aims: To investigate the influence of different culture media and fermentation conditions on growth performance and viability of three lactic acid bacteria (LAB) strains with potential as silage inoculants, and to optimize spray‐drying in order to enhance survival to dehydration and storage stability. Methods and Results: In house‐formulated MRS was a suitable low‐cost culture medium for Lactobacillus plantarum Hv75, Pediococcus acidilactici 3903 and L. buchneri B463. Uncontrolled pH biomass production conferred enhanced stability during storage at 4 °C after spray drying. The use of whey protein concentrate 35 (WPC)‐maltodextrin (M) as matrix, inlet temperature of 145‐150 °C and air flow rate of 601 l h−1 was adequate for the production of dehydrated LAB. According to the desirability function, at this optimized condition, moisture content, yield and solubility were predicted to be 3.96%, 73.68%, and 90.36% respectively. Those conditions also showed a decrease of 0.855 log CFU g‐1 after drying, no loss in viability at 4 °C for 6 months and 1 log CFU g‐1 reduction at 25 °C. Conclusions: Stable and economically feasible dehydrated LAB cultures were obtained by using alternative culture media, fermentation under uncontrolled pH, and optimizing spray‐drying process conditions through the desirability function method.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-25T17:57:54Z
2020-06-25T17:57:54Z
2020-06
info:eu-repo/date/embargoEnd/2021-06-25
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7473
https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/jam.14750
1364-5072
1365-2672
https://doi.org/10.1111/jam.14750
url http://hdl.handle.net/20.500.12123/7473
https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/jam.14750
https://doi.org/10.1111/jam.14750
identifier_str_mv 1364-5072
1365-2672
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Journal of Applied Microbiology (First published:16 June 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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