Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis

Autores
Saraullo, Vanina Rosa; Grune Loffler, Sylvia; Florin-Christensen, Mónica; Watanabe, Olivia; Hamer, Micaela; Martinez, Mara Leila; Brihuega, Bibiana Felicitas
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leptospirosis is the most widespread zoonosis worldwide, and it can cause reproductive failures in livestock, while in humans may vary from a mild fever to multi-organ failure and death. Due to this, in this study, we evaluated the usefulness of the segment encoding LigB C-terminus region, only present in pathogenic as target for a diagnostic PCR. This new PCR yielded a 100 % positivity for pathogenic Leptospira species and no cross-reactivity was found with intermediate or non-pathogenic species, or with other microorganisms, demostrating its high analytical specificity. The estimated analytical sensitivity was higher in serum samples than in blood or urine samples (6−9 × 102 lept/mL and 6−9 × 105 and 6−9 × 106 lept/mL, respectively). Multiple sequence alignment of the target region from different pathogenic Leptospira species confirmed that this gene region is highly conserved among these species, with few single nucleotide polymorphisms. The ligb-ct PCR here developed appears as a useful tool for the molecular diagnosis of leptospirosis.
Instituto de Patobiología
Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Jacobsen, Mónica Ofelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Jacobsen, Mónica Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Watanabe, Olivia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Watanabe, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Comparative Immunology, Microbiology and Infectious Diseases 78 : 101689 (October 2021)
Materia
Leptospirosis
Enfermedades de los Animales
PCR
Diagnóstico
Genética
Animal Diseases
Diagnosis
Genetics
Leptospira
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10776
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oai_identifier_str oai:localhost:20.500.12123/10776
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network_name_str INTA Digital (INTA)
spelling Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosisSaraullo, Vanina RosaGrune Loffler, SylviaFlorin-Christensen, MónicaWatanabe, OliviaHamer, MicaelaMartinez, Mara LeilaBrihuega, Bibiana FelicitasLeptospirosisEnfermedades de los AnimalesPCRDiagnósticoGenéticaAnimal DiseasesDiagnosisGeneticsLeptospiraLeptospirosis is the most widespread zoonosis worldwide, and it can cause reproductive failures in livestock, while in humans may vary from a mild fever to multi-organ failure and death. Due to this, in this study, we evaluated the usefulness of the segment encoding LigB C-terminus region, only present in pathogenic as target for a diagnostic PCR. This new PCR yielded a 100 % positivity for pathogenic Leptospira species and no cross-reactivity was found with intermediate or non-pathogenic species, or with other microorganisms, demostrating its high analytical specificity. The estimated analytical sensitivity was higher in serum samples than in blood or urine samples (6−9 × 102 lept/mL and 6−9 × 105 and 6−9 × 106 lept/mL, respectively). Multiple sequence alignment of the target region from different pathogenic Leptospira species confirmed that this gene region is highly conserved among these species, with few single nucleotide polymorphisms. The ligb-ct PCR here developed appears as a useful tool for the molecular diagnosis of leptospirosis.Instituto de PatobiologíaFil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Jacobsen, Mónica Ofelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Jacobsen, Mónica Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Watanabe, Olivia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Watanabe, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2021-11-15T13:31:05Z2021-11-15T13:31:05Z2021-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10776https://www.sciencedirect.com/science/article/abs/pii/S01479571210008130147-9571https://doi.org/10.1016/j.cimid.2021.101689Comparative Immunology, Microbiology and Infectious Diseases 78 : 101689 (October 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.info:eu-repo/semantics/restrictedAccess2025-11-06T09:41:03Zoai:localhost:20.500.12123/10776instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-06 09:41:03.393INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis
title Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis
spellingShingle Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis
Saraullo, Vanina Rosa
Leptospirosis
Enfermedades de los Animales
PCR
Diagnóstico
Genética
Animal Diseases
Diagnosis
Genetics
Leptospira
title_short Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis
title_full Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis
title_fullStr Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis
title_full_unstemmed Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis
title_sort Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis
dc.creator.none.fl_str_mv Saraullo, Vanina Rosa
Grune Loffler, Sylvia
Florin-Christensen, Mónica
Watanabe, Olivia
Hamer, Micaela
Martinez, Mara Leila
Brihuega, Bibiana Felicitas
author Saraullo, Vanina Rosa
author_facet Saraullo, Vanina Rosa
Grune Loffler, Sylvia
Florin-Christensen, Mónica
Watanabe, Olivia
Hamer, Micaela
Martinez, Mara Leila
Brihuega, Bibiana Felicitas
author_role author
author2 Grune Loffler, Sylvia
Florin-Christensen, Mónica
Watanabe, Olivia
Hamer, Micaela
Martinez, Mara Leila
Brihuega, Bibiana Felicitas
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Leptospirosis
Enfermedades de los Animales
PCR
Diagnóstico
Genética
Animal Diseases
Diagnosis
Genetics
Leptospira
topic Leptospirosis
Enfermedades de los Animales
PCR
Diagnóstico
Genética
Animal Diseases
Diagnosis
Genetics
Leptospira
dc.description.none.fl_txt_mv Leptospirosis is the most widespread zoonosis worldwide, and it can cause reproductive failures in livestock, while in humans may vary from a mild fever to multi-organ failure and death. Due to this, in this study, we evaluated the usefulness of the segment encoding LigB C-terminus region, only present in pathogenic as target for a diagnostic PCR. This new PCR yielded a 100 % positivity for pathogenic Leptospira species and no cross-reactivity was found with intermediate or non-pathogenic species, or with other microorganisms, demostrating its high analytical specificity. The estimated analytical sensitivity was higher in serum samples than in blood or urine samples (6−9 × 102 lept/mL and 6−9 × 105 and 6−9 × 106 lept/mL, respectively). Multiple sequence alignment of the target region from different pathogenic Leptospira species confirmed that this gene region is highly conserved among these species, with few single nucleotide polymorphisms. The ligb-ct PCR here developed appears as a useful tool for the molecular diagnosis of leptospirosis.
Instituto de Patobiología
Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Jacobsen, Mónica Ofelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Jacobsen, Mónica Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Watanabe, Olivia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Watanabe, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Leptospirosis is the most widespread zoonosis worldwide, and it can cause reproductive failures in livestock, while in humans may vary from a mild fever to multi-organ failure and death. Due to this, in this study, we evaluated the usefulness of the segment encoding LigB C-terminus region, only present in pathogenic as target for a diagnostic PCR. This new PCR yielded a 100 % positivity for pathogenic Leptospira species and no cross-reactivity was found with intermediate or non-pathogenic species, or with other microorganisms, demostrating its high analytical specificity. The estimated analytical sensitivity was higher in serum samples than in blood or urine samples (6−9 × 102 lept/mL and 6−9 × 105 and 6−9 × 106 lept/mL, respectively). Multiple sequence alignment of the target region from different pathogenic Leptospira species confirmed that this gene region is highly conserved among these species, with few single nucleotide polymorphisms. The ligb-ct PCR here developed appears as a useful tool for the molecular diagnosis of leptospirosis.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-15T13:31:05Z
2021-11-15T13:31:05Z
2021-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10776
https://www.sciencedirect.com/science/article/abs/pii/S0147957121000813
0147-9571
https://doi.org/10.1016/j.cimid.2021.101689
url http://hdl.handle.net/20.500.12123/10776
https://www.sciencedirect.com/science/article/abs/pii/S0147957121000813
https://doi.org/10.1016/j.cimid.2021.101689
identifier_str_mv 0147-9571
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Comparative Immunology, Microbiology and Infectious Diseases 78 : 101689 (October 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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