Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples

Autores
Saraullo, Vanina Rosa; Hamer, Micaela; Esteban, Micaela; Sanchez, Maria Cristina; Brihuega, Bibiana Felicitas; Martinez, Mara Leila
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leptospirosis is a significant infectious disease affecting livestock. Microscopic agglutination test (MAT) is used as the gold standard; however, the inclusion of molecular tools (PCR) could improve the accuracy of diagnosis. We evaluated the combination of MAT and a LigB-PCR in bovine serum to detect positive cases in animals clinically suspected of leptospirosis. 127 samples were classified by MAT (cut-off 1:100). Of 45 negative samples negative by MAT, 42.2 % were positive by PCR. In addition, the number of confirmed cases of bovine leptospirosis based on clinical suspicion and MAT and/or PCR increased the diagnostic rate to 79.5 %. LigB-PCR can detect traces of leptospiral DNA in serum with negative or low antibody titers and could be used as a complementary tool to MAT.
Instituto de Patobiología
Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina
Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Mara Leila. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina
Fuente
Research in Veterinary Science 187 : 105602 (May 2025)
Materia
PCR
Leptospirosis
Diagnosis
Agglutination Tests
Microscopy
Bovinae
Immunoenzyme Techniques
Diagnóstico
Reacción de Aglutinación
Microscopia
Técnica Inmunoenzimática
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21952
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oai_identifier_str oai:localhost:20.500.12123/21952
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samplesSaraullo, Vanina RosaHamer, MicaelaEsteban, MicaelaSanchez, Maria CristinaBrihuega, Bibiana FelicitasMartinez, Mara LeilaPCRLeptospirosisDiagnosisAgglutination TestsMicroscopyBovinaeImmunoenzyme TechniquesDiagnósticoReacción de AglutinaciónMicroscopiaTécnica InmunoenzimáticaLeptospirosis is a significant infectious disease affecting livestock. Microscopic agglutination test (MAT) is used as the gold standard; however, the inclusion of molecular tools (PCR) could improve the accuracy of diagnosis. We evaluated the combination of MAT and a LigB-PCR in bovine serum to detect positive cases in animals clinically suspected of leptospirosis. 127 samples were classified by MAT (cut-off 1:100). Of 45 negative samples negative by MAT, 42.2 % were positive by PCR. In addition, the number of confirmed cases of bovine leptospirosis based on clinical suspicion and MAT and/or PCR increased the diagnostic rate to 79.5 %. LigB-PCR can detect traces of leptospiral DNA in serum with negative or low antibody titers and could be used as a complementary tool to MAT.Instituto de PatobiologíaFil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; ArgentinaFil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martinez, Mara Leila. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; ArgentinaElsevier2025-04-08T12:46:54Z2025-04-08T12:46:54Z2025-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21952https://www.sciencedirect.com/science/article/abs/pii/S00345288250007611532-2661https://doi.org/10.1016/j.rvsc.2025.105602Research in Veterinary Science 187 : 105602 (May 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:51:00Zoai:localhost:20.500.12123/21952instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:51:00.552INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples
title Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples
spellingShingle Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples
Saraullo, Vanina Rosa
PCR
Leptospirosis
Diagnosis
Agglutination Tests
Microscopy
Bovinae
Immunoenzyme Techniques
Diagnóstico
Reacción de Aglutinación
Microscopia
Técnica Inmunoenzimática
title_short Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples
title_full Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples
title_fullStr Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples
title_full_unstemmed Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples
title_sort Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples
dc.creator.none.fl_str_mv Saraullo, Vanina Rosa
Hamer, Micaela
Esteban, Micaela
Sanchez, Maria Cristina
Brihuega, Bibiana Felicitas
Martinez, Mara Leila
author Saraullo, Vanina Rosa
author_facet Saraullo, Vanina Rosa
Hamer, Micaela
Esteban, Micaela
Sanchez, Maria Cristina
Brihuega, Bibiana Felicitas
Martinez, Mara Leila
author_role author
author2 Hamer, Micaela
Esteban, Micaela
Sanchez, Maria Cristina
Brihuega, Bibiana Felicitas
Martinez, Mara Leila
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv PCR
Leptospirosis
Diagnosis
Agglutination Tests
Microscopy
Bovinae
Immunoenzyme Techniques
Diagnóstico
Reacción de Aglutinación
Microscopia
Técnica Inmunoenzimática
topic PCR
Leptospirosis
Diagnosis
Agglutination Tests
Microscopy
Bovinae
Immunoenzyme Techniques
Diagnóstico
Reacción de Aglutinación
Microscopia
Técnica Inmunoenzimática
dc.description.none.fl_txt_mv Leptospirosis is a significant infectious disease affecting livestock. Microscopic agglutination test (MAT) is used as the gold standard; however, the inclusion of molecular tools (PCR) could improve the accuracy of diagnosis. We evaluated the combination of MAT and a LigB-PCR in bovine serum to detect positive cases in animals clinically suspected of leptospirosis. 127 samples were classified by MAT (cut-off 1:100). Of 45 negative samples negative by MAT, 42.2 % were positive by PCR. In addition, the number of confirmed cases of bovine leptospirosis based on clinical suspicion and MAT and/or PCR increased the diagnostic rate to 79.5 %. LigB-PCR can detect traces of leptospiral DNA in serum with negative or low antibody titers and could be used as a complementary tool to MAT.
Instituto de Patobiología
Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina
Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Mara Leila. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina
description Leptospirosis is a significant infectious disease affecting livestock. Microscopic agglutination test (MAT) is used as the gold standard; however, the inclusion of molecular tools (PCR) could improve the accuracy of diagnosis. We evaluated the combination of MAT and a LigB-PCR in bovine serum to detect positive cases in animals clinically suspected of leptospirosis. 127 samples were classified by MAT (cut-off 1:100). Of 45 negative samples negative by MAT, 42.2 % were positive by PCR. In addition, the number of confirmed cases of bovine leptospirosis based on clinical suspicion and MAT and/or PCR increased the diagnostic rate to 79.5 %. LigB-PCR can detect traces of leptospiral DNA in serum with negative or low antibody titers and could be used as a complementary tool to MAT.
publishDate 2025
dc.date.none.fl_str_mv 2025-04-08T12:46:54Z
2025-04-08T12:46:54Z
2025-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21952
https://www.sciencedirect.com/science/article/abs/pii/S0034528825000761
1532-2661
https://doi.org/10.1016/j.rvsc.2025.105602
url http://hdl.handle.net/20.500.12123/21952
https://www.sciencedirect.com/science/article/abs/pii/S0034528825000761
https://doi.org/10.1016/j.rvsc.2025.105602
identifier_str_mv 1532-2661
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Research in Veterinary Science 187 : 105602 (May 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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