Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia

Autores
Ágredo-Campos, Ángela Sofía; Camussone, Cecilia; Akineden, Ömer; Fernández-Silva, Jorge A.; Ramírez-Vásquez, Nicolás F.
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Staphylococcus aureus is recognized for causing contagious mastitis in cattle. Elimination of the organism in affected animals is challenging due to its potential antibiotic resistance mechanisms and virulence factors. The aim of the study was the pheno- and genotypic epidemiological characterization of S. aureus isolated from bulk tank milk samples obtained from three municipalities in the northern region of Antioquia. Twenty-one S. aureus isolates from 150 bulk tank milk samples were characterized by evaluating antimicrobial susceptibility to 17 antibiotics using the VITEK 2, and identifying several genes encoding for virulence factors, enterotoxins, and the agr groups by conventional PCR. The clonal relationship between the isolates was assessed using macrorestriction fragment analysis (MRFA) of chromosomal DNA by pulsed-field gel electrophoresis (PFGE). Most of the isolates showed susceptibility to the tested antibiotics; 19% exhibited resistance to tetracycline and all isolates showed beta-lactamases. Molecular typing revealed that 76% carried the agrI and cap5 genes. Seven isolates harbored genes coding for staphylococcal enterotoxins (SE), with seh being the most identified gene. Furthermore, MRFA demonstrated high heterogeneity among the isolates, resulting in the assignment of 18 different MRFA patterns. These results indicate phenotypic resistance to tetracycline and beta-lactams, the presence of genes encoding for staphylococcal enterotoxins, and high heterogeneity among S. aureus isolates from bulk tank milk.
EEA Rafaela
Fil: Ágredo-Campos, Ángela Sofía. Universidad de Antioquia. Facultad de Ciencias Agrarias. Escuela de Medicina Veterinaria. Grupo Centauro; Colombia
Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Akineden, Ömer. Justus-Liebig-University Giessen. Institute of Veterinary Food Science. Dairy Sciences; Alemania
Fil: Fernández-Silva, Jorge A. Universidad de Antioquia. Facultad de Ciencias Agrarias. Escuela de Medicina Veterinaria. Grupo Centauro; Colombia
Fil: Ramírez-Vásquez, Nicolás F. Universidad de Antioquia. Facultad de Ciencias Agrarias. Escuela de Medicina Veterinaria. Grupo Centauro; Colombia
Fuente
International Dairy Journal 160 : 106088. (January 2025)
Materia
Staphylococcus aureus
Epidemiology
Milk
Phenology
Bovine Mastitis
Epidemiología
Leche
Fenología
Mastítis Bovina
Colombia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21541
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oai_identifier_str oai:localhost:20.500.12123/21541
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in ColombiaÁgredo-Campos, Ángela SofíaCamussone, CeciliaAkineden, ÖmerFernández-Silva, Jorge A.Ramírez-Vásquez, Nicolás F.Staphylococcus aureusEpidemiologyMilkPhenologyBovine MastitisEpidemiologíaLecheFenologíaMastítis BovinaColombiaStaphylococcus aureus is recognized for causing contagious mastitis in cattle. Elimination of the organism in affected animals is challenging due to its potential antibiotic resistance mechanisms and virulence factors. The aim of the study was the pheno- and genotypic epidemiological characterization of S. aureus isolated from bulk tank milk samples obtained from three municipalities in the northern region of Antioquia. Twenty-one S. aureus isolates from 150 bulk tank milk samples were characterized by evaluating antimicrobial susceptibility to 17 antibiotics using the VITEK 2, and identifying several genes encoding for virulence factors, enterotoxins, and the agr groups by conventional PCR. The clonal relationship between the isolates was assessed using macrorestriction fragment analysis (MRFA) of chromosomal DNA by pulsed-field gel electrophoresis (PFGE). Most of the isolates showed susceptibility to the tested antibiotics; 19% exhibited resistance to tetracycline and all isolates showed beta-lactamases. Molecular typing revealed that 76% carried the agrI and cap5 genes. Seven isolates harbored genes coding for staphylococcal enterotoxins (SE), with seh being the most identified gene. Furthermore, MRFA demonstrated high heterogeneity among the isolates, resulting in the assignment of 18 different MRFA patterns. These results indicate phenotypic resistance to tetracycline and beta-lactams, the presence of genes encoding for staphylococcal enterotoxins, and high heterogeneity among S. aureus isolates from bulk tank milk.EEA RafaelaFil: Ágredo-Campos, Ángela Sofía. Universidad de Antioquia. Facultad de Ciencias Agrarias. Escuela de Medicina Veterinaria. Grupo Centauro; ColombiaFil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Akineden, Ömer. Justus-Liebig-University Giessen. Institute of Veterinary Food Science. Dairy Sciences; AlemaniaFil: Fernández-Silva, Jorge A. Universidad de Antioquia. Facultad de Ciencias Agrarias. Escuela de Medicina Veterinaria. Grupo Centauro; ColombiaFil: Ramírez-Vásquez, Nicolás F. Universidad de Antioquia. Facultad de Ciencias Agrarias. Escuela de Medicina Veterinaria. Grupo Centauro; ColombiaElsevier2025-03-05T13:55:50Z2025-03-05T13:55:50Z2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21541https://www.sciencedirect.com/science/article/pii/S09586946240020850958-69461879-0143https://doi.org/10.1016/j.idairyj.2024.106088International Dairy Journal 160 : 106088. (January 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:57Zoai:localhost:20.500.12123/21541instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:58.041INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
title Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
spellingShingle Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
Ágredo-Campos, Ángela Sofía
Staphylococcus aureus
Epidemiology
Milk
Phenology
Bovine Mastitis
Epidemiología
Leche
Fenología
Mastítis Bovina
Colombia
title_short Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
title_full Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
title_fullStr Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
title_full_unstemmed Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
title_sort Pheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombia
dc.creator.none.fl_str_mv Ágredo-Campos, Ángela Sofía
Camussone, Cecilia
Akineden, Ömer
Fernández-Silva, Jorge A.
Ramírez-Vásquez, Nicolás F.
author Ágredo-Campos, Ángela Sofía
author_facet Ágredo-Campos, Ángela Sofía
Camussone, Cecilia
Akineden, Ömer
Fernández-Silva, Jorge A.
Ramírez-Vásquez, Nicolás F.
author_role author
author2 Camussone, Cecilia
Akineden, Ömer
Fernández-Silva, Jorge A.
Ramírez-Vásquez, Nicolás F.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Staphylococcus aureus
Epidemiology
Milk
Phenology
Bovine Mastitis
Epidemiología
Leche
Fenología
Mastítis Bovina
Colombia
topic Staphylococcus aureus
Epidemiology
Milk
Phenology
Bovine Mastitis
Epidemiología
Leche
Fenología
Mastítis Bovina
Colombia
dc.description.none.fl_txt_mv Staphylococcus aureus is recognized for causing contagious mastitis in cattle. Elimination of the organism in affected animals is challenging due to its potential antibiotic resistance mechanisms and virulence factors. The aim of the study was the pheno- and genotypic epidemiological characterization of S. aureus isolated from bulk tank milk samples obtained from three municipalities in the northern region of Antioquia. Twenty-one S. aureus isolates from 150 bulk tank milk samples were characterized by evaluating antimicrobial susceptibility to 17 antibiotics using the VITEK 2, and identifying several genes encoding for virulence factors, enterotoxins, and the agr groups by conventional PCR. The clonal relationship between the isolates was assessed using macrorestriction fragment analysis (MRFA) of chromosomal DNA by pulsed-field gel electrophoresis (PFGE). Most of the isolates showed susceptibility to the tested antibiotics; 19% exhibited resistance to tetracycline and all isolates showed beta-lactamases. Molecular typing revealed that 76% carried the agrI and cap5 genes. Seven isolates harbored genes coding for staphylococcal enterotoxins (SE), with seh being the most identified gene. Furthermore, MRFA demonstrated high heterogeneity among the isolates, resulting in the assignment of 18 different MRFA patterns. These results indicate phenotypic resistance to tetracycline and beta-lactams, the presence of genes encoding for staphylococcal enterotoxins, and high heterogeneity among S. aureus isolates from bulk tank milk.
EEA Rafaela
Fil: Ágredo-Campos, Ángela Sofía. Universidad de Antioquia. Facultad de Ciencias Agrarias. Escuela de Medicina Veterinaria. Grupo Centauro; Colombia
Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Akineden, Ömer. Justus-Liebig-University Giessen. Institute of Veterinary Food Science. Dairy Sciences; Alemania
Fil: Fernández-Silva, Jorge A. Universidad de Antioquia. Facultad de Ciencias Agrarias. Escuela de Medicina Veterinaria. Grupo Centauro; Colombia
Fil: Ramírez-Vásquez, Nicolás F. Universidad de Antioquia. Facultad de Ciencias Agrarias. Escuela de Medicina Veterinaria. Grupo Centauro; Colombia
description Staphylococcus aureus is recognized for causing contagious mastitis in cattle. Elimination of the organism in affected animals is challenging due to its potential antibiotic resistance mechanisms and virulence factors. The aim of the study was the pheno- and genotypic epidemiological characterization of S. aureus isolated from bulk tank milk samples obtained from three municipalities in the northern region of Antioquia. Twenty-one S. aureus isolates from 150 bulk tank milk samples were characterized by evaluating antimicrobial susceptibility to 17 antibiotics using the VITEK 2, and identifying several genes encoding for virulence factors, enterotoxins, and the agr groups by conventional PCR. The clonal relationship between the isolates was assessed using macrorestriction fragment analysis (MRFA) of chromosomal DNA by pulsed-field gel electrophoresis (PFGE). Most of the isolates showed susceptibility to the tested antibiotics; 19% exhibited resistance to tetracycline and all isolates showed beta-lactamases. Molecular typing revealed that 76% carried the agrI and cap5 genes. Seven isolates harbored genes coding for staphylococcal enterotoxins (SE), with seh being the most identified gene. Furthermore, MRFA demonstrated high heterogeneity among the isolates, resulting in the assignment of 18 different MRFA patterns. These results indicate phenotypic resistance to tetracycline and beta-lactams, the presence of genes encoding for staphylococcal enterotoxins, and high heterogeneity among S. aureus isolates from bulk tank milk.
publishDate 2025
dc.date.none.fl_str_mv 2025-03-05T13:55:50Z
2025-03-05T13:55:50Z
2025-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21541
https://www.sciencedirect.com/science/article/pii/S0958694624002085
0958-6946
1879-0143
https://doi.org/10.1016/j.idairyj.2024.106088
url http://hdl.handle.net/20.500.12123/21541
https://www.sciencedirect.com/science/article/pii/S0958694624002085
https://doi.org/10.1016/j.idairyj.2024.106088
identifier_str_mv 0958-6946
1879-0143
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv International Dairy Journal 160 : 106088. (January 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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