Ecology and evolution of influenza A viruses in wild waterfowl in Argentina reveals novel viral genotypes

Autores
Kreps, Lily; Ortiz Batsche, Lucia; Olivera, Valeria Soledad; Crespo-Bellido, Alvin; Nelson, Martha I.; Perez, Daniel R.; Rimondi, Agustina
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study investigates the genetic diversity of influenza A viruses (IAVs) in wild birds in Argentina prior to the 2023 outbreak of highly pathogenic avian influenza (HPAI) H5N1. Between 2017 and 2019, 2521 samples were collected from 39 bird species, and viral genomes (n = 44) were sequenced from nine duck species, including a newly identified host in South America, the ringed teal (Callonetta leucophrys). We detected five IAV subtypes for the first time in Argentina: H2N1, H3N8, H7N3, H8N4, and H11N9. Additionally, we identified a previously unrecognized South American H8 lineage that diverged from the North American lineage approximately 50 years ago. Phylogenetic analysis revealed a unique genetic profile: while the viruses’ core internal segments were exclusively from the South American lineage, the surface segments (hemagglutinin and neuraminidase) exhibited reassortment between North and South American lineages. This resulted in novel, Argentina-specific genotypes not observed in other countries in the region. The recent arrival of HPAI H5N1 in South America raises serious concerns about potential reassortment with these unique Argentinian strains, which could create new HPAI viruses with unpredictable characteristics. This study highlights the unique evolutionary dynamics of IAVs in Argentina and emphasizes the need for ongoing influenza surveillance in under-studied regions in South America to monitor these evolving viral populations.
Instituto de Virología
Fil: Kreps, Lily. National Institutes of Health. National Library of Medicine. Division of Intramural Research; Estados Unidos
Fil: Ortiz Batsche, Lucia. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
Fil: Ortiz Batsche, Lucia. Emory University. Rollins Research Center. Department of Microbiology & Immunology; Estados Unidos
Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Olivera, Valeria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Crespo-Bellido, Alvin. National Institutes of Health. National Library of Medicine. Division of Intramural Research; Estados Unidos
Fil: Nelson, Martha I. National Institutes of Health. National Library of Medicine. Division of Intramural Research; Estados Unidos
Fil: Perez, Daniel R. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
Fil: Rimondi, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Rimondi, Agustina. Consejo Nacional de Investigaciones Científcas y Técnicas; Argentina
Fil: Rimondi, Agustina. Robert Koch Institute.Influenza and Other Respiratory Viruses. Unit 17; Alemania
Fuente
Virus Evolution 11 (1) : veaf088 (November 2025)
Materia
Ave Acuática de Agua Dulce
Virus de la Influenza Aviar
Argentina
Waterfowl
Genotypes
Influenzavirus
Avian Influenzavirus
Genotipos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25364
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oai_identifier_str oai:localhost:20.500.12123/25364
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spelling Ecology and evolution of influenza A viruses in wild waterfowl in Argentina reveals novel viral genotypesKreps, LilyOrtiz Batsche, LuciaOlivera, Valeria SoledadCrespo-Bellido, AlvinNelson, Martha I.Perez, Daniel R.Rimondi, AgustinaAve Acuática de Agua DulceVirus de la Influenza AviarArgentinaWaterfowlGenotypesInfluenzavirusAvian InfluenzavirusGenotiposThis study investigates the genetic diversity of influenza A viruses (IAVs) in wild birds in Argentina prior to the 2023 outbreak of highly pathogenic avian influenza (HPAI) H5N1. Between 2017 and 2019, 2521 samples were collected from 39 bird species, and viral genomes (n = 44) were sequenced from nine duck species, including a newly identified host in South America, the ringed teal (Callonetta leucophrys). We detected five IAV subtypes for the first time in Argentina: H2N1, H3N8, H7N3, H8N4, and H11N9. Additionally, we identified a previously unrecognized South American H8 lineage that diverged from the North American lineage approximately 50 years ago. Phylogenetic analysis revealed a unique genetic profile: while the viruses’ core internal segments were exclusively from the South American lineage, the surface segments (hemagglutinin and neuraminidase) exhibited reassortment between North and South American lineages. This resulted in novel, Argentina-specific genotypes not observed in other countries in the region. The recent arrival of HPAI H5N1 in South America raises serious concerns about potential reassortment with these unique Argentinian strains, which could create new HPAI viruses with unpredictable characteristics. This study highlights the unique evolutionary dynamics of IAVs in Argentina and emphasizes the need for ongoing influenza surveillance in under-studied regions in South America to monitor these evolving viral populations.Instituto de VirologíaFil: Kreps, Lily. National Institutes of Health. National Library of Medicine. Division of Intramural Research; Estados UnidosFil: Ortiz Batsche, Lucia. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados UnidosFil: Ortiz Batsche, Lucia. Emory University. Rollins Research Center. Department of Microbiology & Immunology; Estados UnidosFil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Olivera, Valeria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Crespo-Bellido, Alvin. National Institutes of Health. National Library of Medicine. Division of Intramural Research; Estados UnidosFil: Nelson, Martha I. National Institutes of Health. National Library of Medicine. Division of Intramural Research; Estados UnidosFil: Perez, Daniel R. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados UnidosFil: Rimondi, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Rimondi, Agustina. Consejo Nacional de Investigaciones Científcas y Técnicas; ArgentinaFil: Rimondi, Agustina. Robert Koch Institute.Influenza and Other Respiratory Viruses. Unit 17; AlemaniaOxford University Press2026-03-05T15:04:36Z2026-03-05T15:04:36Z2025-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25364https://academic.oup.com/ve/article/11/1/veaf088/83158402057-1577https://doi.org/10.1093/ve/veaf088Virus Evolution 11 (1) : veaf088 (November 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNSA/1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.info:eu-repograntAgreement/INTA/PNSA-1115056/AR./Enfermedades infecciosas de las avesinfo:eu-repograntAgreement/INTA/2019-PD-E5-I103-001, Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud públicainfo:eu-repograntAgreement/INTA/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud?info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Saludinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-04-01T11:49:57Zoai:localhost:20.500.12123/25364instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-04-01 11:49:57.857INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Ecology and evolution of influenza A viruses in wild waterfowl in Argentina reveals novel viral genotypes
title Ecology and evolution of influenza A viruses in wild waterfowl in Argentina reveals novel viral genotypes
spellingShingle Ecology and evolution of influenza A viruses in wild waterfowl in Argentina reveals novel viral genotypes
Kreps, Lily
Ave Acuática de Agua Dulce
Virus de la Influenza Aviar
Argentina
Waterfowl
Genotypes
Influenzavirus
Avian Influenzavirus
Genotipos
title_short Ecology and evolution of influenza A viruses in wild waterfowl in Argentina reveals novel viral genotypes
title_full Ecology and evolution of influenza A viruses in wild waterfowl in Argentina reveals novel viral genotypes
title_fullStr Ecology and evolution of influenza A viruses in wild waterfowl in Argentina reveals novel viral genotypes
title_full_unstemmed Ecology and evolution of influenza A viruses in wild waterfowl in Argentina reveals novel viral genotypes
title_sort Ecology and evolution of influenza A viruses in wild waterfowl in Argentina reveals novel viral genotypes
dc.creator.none.fl_str_mv Kreps, Lily
Ortiz Batsche, Lucia
Olivera, Valeria Soledad
Crespo-Bellido, Alvin
Nelson, Martha I.
Perez, Daniel R.
Rimondi, Agustina
author Kreps, Lily
author_facet Kreps, Lily
Ortiz Batsche, Lucia
Olivera, Valeria Soledad
Crespo-Bellido, Alvin
Nelson, Martha I.
Perez, Daniel R.
Rimondi, Agustina
author_role author
author2 Ortiz Batsche, Lucia
Olivera, Valeria Soledad
Crespo-Bellido, Alvin
Nelson, Martha I.
Perez, Daniel R.
Rimondi, Agustina
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ave Acuática de Agua Dulce
Virus de la Influenza Aviar
Argentina
Waterfowl
Genotypes
Influenzavirus
Avian Influenzavirus
Genotipos
topic Ave Acuática de Agua Dulce
Virus de la Influenza Aviar
Argentina
Waterfowl
Genotypes
Influenzavirus
Avian Influenzavirus
Genotipos
dc.description.none.fl_txt_mv This study investigates the genetic diversity of influenza A viruses (IAVs) in wild birds in Argentina prior to the 2023 outbreak of highly pathogenic avian influenza (HPAI) H5N1. Between 2017 and 2019, 2521 samples were collected from 39 bird species, and viral genomes (n = 44) were sequenced from nine duck species, including a newly identified host in South America, the ringed teal (Callonetta leucophrys). We detected five IAV subtypes for the first time in Argentina: H2N1, H3N8, H7N3, H8N4, and H11N9. Additionally, we identified a previously unrecognized South American H8 lineage that diverged from the North American lineage approximately 50 years ago. Phylogenetic analysis revealed a unique genetic profile: while the viruses’ core internal segments were exclusively from the South American lineage, the surface segments (hemagglutinin and neuraminidase) exhibited reassortment between North and South American lineages. This resulted in novel, Argentina-specific genotypes not observed in other countries in the region. The recent arrival of HPAI H5N1 in South America raises serious concerns about potential reassortment with these unique Argentinian strains, which could create new HPAI viruses with unpredictable characteristics. This study highlights the unique evolutionary dynamics of IAVs in Argentina and emphasizes the need for ongoing influenza surveillance in under-studied regions in South America to monitor these evolving viral populations.
Instituto de Virología
Fil: Kreps, Lily. National Institutes of Health. National Library of Medicine. Division of Intramural Research; Estados Unidos
Fil: Ortiz Batsche, Lucia. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
Fil: Ortiz Batsche, Lucia. Emory University. Rollins Research Center. Department of Microbiology & Immunology; Estados Unidos
Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Olivera, Valeria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Crespo-Bellido, Alvin. National Institutes of Health. National Library of Medicine. Division of Intramural Research; Estados Unidos
Fil: Nelson, Martha I. National Institutes of Health. National Library of Medicine. Division of Intramural Research; Estados Unidos
Fil: Perez, Daniel R. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
Fil: Rimondi, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Rimondi, Agustina. Consejo Nacional de Investigaciones Científcas y Técnicas; Argentina
Fil: Rimondi, Agustina. Robert Koch Institute.Influenza and Other Respiratory Viruses. Unit 17; Alemania
description This study investigates the genetic diversity of influenza A viruses (IAVs) in wild birds in Argentina prior to the 2023 outbreak of highly pathogenic avian influenza (HPAI) H5N1. Between 2017 and 2019, 2521 samples were collected from 39 bird species, and viral genomes (n = 44) were sequenced from nine duck species, including a newly identified host in South America, the ringed teal (Callonetta leucophrys). We detected five IAV subtypes for the first time in Argentina: H2N1, H3N8, H7N3, H8N4, and H11N9. Additionally, we identified a previously unrecognized South American H8 lineage that diverged from the North American lineage approximately 50 years ago. Phylogenetic analysis revealed a unique genetic profile: while the viruses’ core internal segments were exclusively from the South American lineage, the surface segments (hemagglutinin and neuraminidase) exhibited reassortment between North and South American lineages. This resulted in novel, Argentina-specific genotypes not observed in other countries in the region. The recent arrival of HPAI H5N1 in South America raises serious concerns about potential reassortment with these unique Argentinian strains, which could create new HPAI viruses with unpredictable characteristics. This study highlights the unique evolutionary dynamics of IAVs in Argentina and emphasizes the need for ongoing influenza surveillance in under-studied regions in South America to monitor these evolving viral populations.
publishDate 2025
dc.date.none.fl_str_mv 2025-11
2026-03-05T15:04:36Z
2026-03-05T15:04:36Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25364
https://academic.oup.com/ve/article/11/1/veaf088/8315840
2057-1577
https://doi.org/10.1093/ve/veaf088
url http://hdl.handle.net/20.500.12123/25364
https://academic.oup.com/ve/article/11/1/veaf088/8315840
https://doi.org/10.1093/ve/veaf088
identifier_str_mv 2057-1577
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNSA/1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.
info:eu-repograntAgreement/INTA/PNSA-1115056/AR./Enfermedades infecciosas de las aves
info:eu-repograntAgreement/INTA/2019-PD-E5-I103-001, Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud pública
info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud?
info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv Virus Evolution 11 (1) : veaf088 (November 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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