A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus

Autores
Garcia Guizzo, Melina; Parizi, Luís Fernando; Nunes, Rodrigo Dutra; Schama, Renata; Albano, Rodolpho M.; Tirloni, Lucas; Oldiges, Daiane Patrícia; Pilz Vieira, Ricardo; Cruz Oliveira, Wanderson Henrique; De Souza Leite, Milane; Gonzalez, Sergio Alberto; Farber, Marisa Diana; Martins, Orlando; Da Silva Vaz, Itabajara; Oliveira, Pedro L.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that a Coxiella endosymbiont from R. microplus (CERM) is a vertically transmitted mutualist symbiont, comprising 98% of the 16S rRNA sequences in both eggs and larvae. Sequencing of the bacterial genome revealed genes for biosynthetic pathways for several vitamins and key metabolic cofactors that may provide a nutritional complement to the tick host. The CERM was abundant in ovary and Malpighian tubule of fully engorged female. Tetracycline treatment of either the tick or the vertebrate host reduced levels of bacteria in progeny in 74% for eggs and 90% for larvae without major impact neither on the reproductive fitness of the adult female or on embryo development. However, CERM proved to be essential for the tick to reach the adult life stage, as under antibiotic treatment no tick was able to progress beyond the metanymph stage. Data presented here suggest that interference in the symbiotic CERM-R. microplus relationship may be useful to the development of alternative control methods, highlighting the interdependence between ticks and their endosymbionts.
Instituto de Biotecnología
Fil: Garcia Guizzo, Melina. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil
Fil: Parizi, Luís Fernando. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil
Fil: Nunes, Rodrigo Dutra. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil
Fil: Schama, Renata. Laboratório de Biologia Computacional e Sistemas; Brasil
Fil: Albano, Rodolpho M. Universidade do Estado do Rio de Janeiro. Departamento de Bioquímica; Brasil
Fil: Tirloni, Lucas. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil
Fil: Oldiges, Daiane Patrícia. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil
Fil: Pilz Vieira, Ricardo. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil
Fil: Cruz Oliveira, Wanderson Henrique. Universidade Federal Rural do Rio de Janeiro. Departamento de Química; Brasil
Fil: De Souza Leite, Milane. Universidade Federal Rural do Rio de Janeiro. Departamento de Química; Brasil
Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Martins, Orlando. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil
Fil: Da Silva Vaz, Itabajara. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil. Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária; Brasil. Universidade Federal Rural do Rio de Janeiro. Departamento de Química; Brasil
Fil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil
Fuente
Scientific Reports 7: 17554 (Diciembre 2017)
Materia
Coxiella
Rhipicephalus
Metastigmata
Simbionte
Garrapatas
Symbiont
Ticks
Rhipicephalus microplus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3988
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3988
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplusGarcia Guizzo, MelinaParizi, Luís FernandoNunes, Rodrigo DutraSchama, RenataAlbano, Rodolpho M.Tirloni, LucasOldiges, Daiane PatríciaPilz Vieira, RicardoCruz Oliveira, Wanderson HenriqueDe Souza Leite, MilaneGonzalez, Sergio AlbertoFarber, Marisa DianaMartins, OrlandoDa Silva Vaz, ItabajaraOliveira, Pedro L.CoxiellaRhipicephalusMetastigmataSimbionteGarrapatasSymbiontTicksRhipicephalus microplusThe cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that a Coxiella endosymbiont from R. microplus (CERM) is a vertically transmitted mutualist symbiont, comprising 98% of the 16S rRNA sequences in both eggs and larvae. Sequencing of the bacterial genome revealed genes for biosynthetic pathways for several vitamins and key metabolic cofactors that may provide a nutritional complement to the tick host. The CERM was abundant in ovary and Malpighian tubule of fully engorged female. Tetracycline treatment of either the tick or the vertebrate host reduced levels of bacteria in progeny in 74% for eggs and 90% for larvae without major impact neither on the reproductive fitness of the adult female or on embryo development. However, CERM proved to be essential for the tick to reach the adult life stage, as under antibiotic treatment no tick was able to progress beyond the metanymph stage. Data presented here suggest that interference in the symbiotic CERM-R. microplus relationship may be useful to the development of alternative control methods, highlighting the interdependence between ticks and their endosymbionts.Instituto de BiotecnologíaFil: Garcia Guizzo, Melina. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; BrasilFil: Parizi, Luís Fernando. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; BrasilFil: Nunes, Rodrigo Dutra. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; BrasilFil: Schama, Renata. Laboratório de Biologia Computacional e Sistemas; BrasilFil: Albano, Rodolpho M. Universidade do Estado do Rio de Janeiro. Departamento de Bioquímica; BrasilFil: Tirloni, Lucas. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; BrasilFil: Oldiges, Daiane Patrícia. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; BrasilFil: Pilz Vieira, Ricardo. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; BrasilFil: Cruz Oliveira, Wanderson Henrique. Universidade Federal Rural do Rio de Janeiro. Departamento de Química; BrasilFil: De Souza Leite, Milane. Universidade Federal Rural do Rio de Janeiro. Departamento de Química; BrasilFil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Martins, Orlando. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; BrasilFil: Da Silva Vaz, Itabajara. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil. Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária; Brasil. Universidade Federal Rural do Rio de Janeiro. Departamento de Química; BrasilFil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; BrasilNature Publishing Group2018-11-29T11:13:12Z2018-11-29T11:13:12Z2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.nature.com/articles/s41598-017-17309-xhttp://hdl.handle.net/20.500.12123/39882045-2322https://doi.org/10.1038/s41598-017-17309-xScientific Reports 7: 17554 (Diciembre 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:42Zoai:localhost:20.500.12123/3988instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:43.012INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus
title A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus
spellingShingle A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus
Garcia Guizzo, Melina
Coxiella
Rhipicephalus
Metastigmata
Simbionte
Garrapatas
Symbiont
Ticks
Rhipicephalus microplus
title_short A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus
title_full A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus
title_fullStr A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus
title_full_unstemmed A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus
title_sort A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus
dc.creator.none.fl_str_mv Garcia Guizzo, Melina
Parizi, Luís Fernando
Nunes, Rodrigo Dutra
Schama, Renata
Albano, Rodolpho M.
Tirloni, Lucas
Oldiges, Daiane Patrícia
Pilz Vieira, Ricardo
Cruz Oliveira, Wanderson Henrique
De Souza Leite, Milane
Gonzalez, Sergio Alberto
Farber, Marisa Diana
Martins, Orlando
Da Silva Vaz, Itabajara
Oliveira, Pedro L.
author Garcia Guizzo, Melina
author_facet Garcia Guizzo, Melina
Parizi, Luís Fernando
Nunes, Rodrigo Dutra
Schama, Renata
Albano, Rodolpho M.
Tirloni, Lucas
Oldiges, Daiane Patrícia
Pilz Vieira, Ricardo
Cruz Oliveira, Wanderson Henrique
De Souza Leite, Milane
Gonzalez, Sergio Alberto
Farber, Marisa Diana
Martins, Orlando
Da Silva Vaz, Itabajara
Oliveira, Pedro L.
author_role author
author2 Parizi, Luís Fernando
Nunes, Rodrigo Dutra
Schama, Renata
Albano, Rodolpho M.
Tirloni, Lucas
Oldiges, Daiane Patrícia
Pilz Vieira, Ricardo
Cruz Oliveira, Wanderson Henrique
De Souza Leite, Milane
Gonzalez, Sergio Alberto
Farber, Marisa Diana
Martins, Orlando
Da Silva Vaz, Itabajara
Oliveira, Pedro L.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Coxiella
Rhipicephalus
Metastigmata
Simbionte
Garrapatas
Symbiont
Ticks
Rhipicephalus microplus
topic Coxiella
Rhipicephalus
Metastigmata
Simbionte
Garrapatas
Symbiont
Ticks
Rhipicephalus microplus
dc.description.none.fl_txt_mv The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that a Coxiella endosymbiont from R. microplus (CERM) is a vertically transmitted mutualist symbiont, comprising 98% of the 16S rRNA sequences in both eggs and larvae. Sequencing of the bacterial genome revealed genes for biosynthetic pathways for several vitamins and key metabolic cofactors that may provide a nutritional complement to the tick host. The CERM was abundant in ovary and Malpighian tubule of fully engorged female. Tetracycline treatment of either the tick or the vertebrate host reduced levels of bacteria in progeny in 74% for eggs and 90% for larvae without major impact neither on the reproductive fitness of the adult female or on embryo development. However, CERM proved to be essential for the tick to reach the adult life stage, as under antibiotic treatment no tick was able to progress beyond the metanymph stage. Data presented here suggest that interference in the symbiotic CERM-R. microplus relationship may be useful to the development of alternative control methods, highlighting the interdependence between ticks and their endosymbionts.
Instituto de Biotecnología
Fil: Garcia Guizzo, Melina. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil
Fil: Parizi, Luís Fernando. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil
Fil: Nunes, Rodrigo Dutra. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil
Fil: Schama, Renata. Laboratório de Biologia Computacional e Sistemas; Brasil
Fil: Albano, Rodolpho M. Universidade do Estado do Rio de Janeiro. Departamento de Bioquímica; Brasil
Fil: Tirloni, Lucas. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil
Fil: Oldiges, Daiane Patrícia. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil
Fil: Pilz Vieira, Ricardo. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil
Fil: Cruz Oliveira, Wanderson Henrique. Universidade Federal Rural do Rio de Janeiro. Departamento de Química; Brasil
Fil: De Souza Leite, Milane. Universidade Federal Rural do Rio de Janeiro. Departamento de Química; Brasil
Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Martins, Orlando. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil
Fil: Da Silva Vaz, Itabajara. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil. Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária; Brasil. Universidade Federal Rural do Rio de Janeiro. Departamento de Química; Brasil
Fil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil
description The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that a Coxiella endosymbiont from R. microplus (CERM) is a vertically transmitted mutualist symbiont, comprising 98% of the 16S rRNA sequences in both eggs and larvae. Sequencing of the bacterial genome revealed genes for biosynthetic pathways for several vitamins and key metabolic cofactors that may provide a nutritional complement to the tick host. The CERM was abundant in ovary and Malpighian tubule of fully engorged female. Tetracycline treatment of either the tick or the vertebrate host reduced levels of bacteria in progeny in 74% for eggs and 90% for larvae without major impact neither on the reproductive fitness of the adult female or on embryo development. However, CERM proved to be essential for the tick to reach the adult life stage, as under antibiotic treatment no tick was able to progress beyond the metanymph stage. Data presented here suggest that interference in the symbiotic CERM-R. microplus relationship may be useful to the development of alternative control methods, highlighting the interdependence between ticks and their endosymbionts.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
2018-11-29T11:13:12Z
2018-11-29T11:13:12Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.nature.com/articles/s41598-017-17309-x
http://hdl.handle.net/20.500.12123/3988
2045-2322
https://doi.org/10.1038/s41598-017-17309-x
url https://www.nature.com/articles/s41598-017-17309-x
http://hdl.handle.net/20.500.12123/3988
https://doi.org/10.1038/s41598-017-17309-x
identifier_str_mv 2045-2322
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Scientific Reports 7: 17554 (Diciembre 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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