RNAi Expression in Cotton for Control of Herbivorous Insects

Autores
Salvador, Ricardo; Maskin, Laura; Niz, José María; Turica, Mariana Daniela; Pedarros, Analía Soledad; Hopp, Horacio Esteban; Lewi, Dalia Marcela
Año de publicación
2022
Idioma
inglés
Tipo de recurso
parte de libro
Estado
versión publicada
Descripción
Cultivated cotton (Gossypium hirsutum) is heavily attacked by various species of insects worldwide and breeding of new varieties resistant to pests is still a hard battle to win. RNAi technology is an important reverse genetics tool to induce gene silencing in eukaryotic organisms and produce phenotypic modifications. In cotton, RNAi was applied to investigate gene function and enhance resistance to insects and pathogens. Different methods and techniques can be used to synthetize double stranded RNA (dsRNA) into plant cells. The Agrobacterium-mediated transformation is a common method to introduce RNAi binary plasmids into cotton genome and obtain stable transgenics plants. This methodology includes the coculture of cotton tissues with Agrobacterium cultures, selection of transgenic cells and induction of somatic embryogenesis to finally obtain transgenic plants after a relatively long period of time. The transient synthesis of dsRNA mediated by virus-induced gene silencing (VIGS) in cotton is an alternative to anticipate the silencing effect of a specific RNA sequence, prior to the development of a stable transgenic plant. VIGS vectors are incorporated into the plant by agroinfiltration technique. During VIGS replication inside plant cells, synthetized dsRNA allows the study on specific heterologous gene expression including the phenotypic effect on herbivorous target pests, thus facilitating a rapid evaluation of dsRNA expressed in cotton plants against individual insect target genes. Here we describe the complementation of these two techniques to evaluate RNAi-based cotton plant protection against insect pests.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Maskin, Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Niz, José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Turica, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Pedarros, Analía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Agrobiotecnología; Argentina
Fil: Lewi, Dalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fuente
RNAi Strategies for Pest Management : methods and protocols / editors: Luis María Vaschetto. New York: Humana, 2021. p. 217–233
Materia
Gossypium hirsutum
Algodón
Cotton
Biological Control
Pest Control
Control Biológico
Control de Plagas
Transgenic Cotton Plants
Virus Induced Gene Silencing
RNA Interference
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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network_name_str INTA Digital (INTA)
spelling RNAi Expression in Cotton for Control of Herbivorous InsectsSalvador, RicardoMaskin, LauraNiz, José MaríaTurica, Mariana DanielaPedarros, Analía SoledadHopp, Horacio EstebanLewi, Dalia MarcelaGossypium hirsutumAlgodónCottonBiological ControlPest ControlControl BiológicoControl de PlagasTransgenic Cotton PlantsVirus Induced Gene SilencingRNA InterferenceCultivated cotton (Gossypium hirsutum) is heavily attacked by various species of insects worldwide and breeding of new varieties resistant to pests is still a hard battle to win. RNAi technology is an important reverse genetics tool to induce gene silencing in eukaryotic organisms and produce phenotypic modifications. In cotton, RNAi was applied to investigate gene function and enhance resistance to insects and pathogens. Different methods and techniques can be used to synthetize double stranded RNA (dsRNA) into plant cells. The Agrobacterium-mediated transformation is a common method to introduce RNAi binary plasmids into cotton genome and obtain stable transgenics plants. This methodology includes the coculture of cotton tissues with Agrobacterium cultures, selection of transgenic cells and induction of somatic embryogenesis to finally obtain transgenic plants after a relatively long period of time. The transient synthesis of dsRNA mediated by virus-induced gene silencing (VIGS) in cotton is an alternative to anticipate the silencing effect of a specific RNA sequence, prior to the development of a stable transgenic plant. VIGS vectors are incorporated into the plant by agroinfiltration technique. During VIGS replication inside plant cells, synthetized dsRNA allows the study on specific heterologous gene expression including the phenotypic effect on herbivorous target pests, thus facilitating a rapid evaluation of dsRNA expressed in cotton plants against individual insect target genes. Here we describe the complementation of these two techniques to evaluate RNAi-based cotton plant protection against insect pests.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Maskin, Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Niz, José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Turica, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Pedarros, Analía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Agrobiotecnología; ArgentinaFil: Lewi, Dalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaSpringer2022-07-26T22:43:52Z2022-07-26T22:43:52Z2022info:eu-repo/semantics/bookPartinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_3248info:ar-repo/semantics/parteDeLibroapplication/pdfhttp://hdl.handle.net/20.500.12123/12411https://link.springer.com/protocol/10.1007/978-1-0716-1633-8_17Salvador, R. et al. (2022). RNAi Expression in Cotton for Control of Herbivorous Insects. pp 217-233. In: Vaschetto, L.M. (eds) RNAi Strategies for Pest Management. Methods in Molecular Biology, vol 2360. Humana, New York, NY978-1-0716-1633-8https://doi.org/10.1007/978-1-0716-1633-8_17RNAi Strategies for Pest Management : methods and protocols / editors: Luis María Vaschetto. New York: Humana, 2021. p. 217–233reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengMethods in Molecular Biology (Springer)info:eu-repo/semantics/restrictedAccess2026-05-07T11:48:44Zoai:localhost:20.500.12123/12411instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-05-07 11:48:45.936INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv RNAi Expression in Cotton for Control of Herbivorous Insects
title RNAi Expression in Cotton for Control of Herbivorous Insects
spellingShingle RNAi Expression in Cotton for Control of Herbivorous Insects
Salvador, Ricardo
Gossypium hirsutum
Algodón
Cotton
Biological Control
Pest Control
Control Biológico
Control de Plagas
Transgenic Cotton Plants
Virus Induced Gene Silencing
RNA Interference
title_short RNAi Expression in Cotton for Control of Herbivorous Insects
title_full RNAi Expression in Cotton for Control of Herbivorous Insects
title_fullStr RNAi Expression in Cotton for Control of Herbivorous Insects
title_full_unstemmed RNAi Expression in Cotton for Control of Herbivorous Insects
title_sort RNAi Expression in Cotton for Control of Herbivorous Insects
dc.creator.none.fl_str_mv Salvador, Ricardo
Maskin, Laura
Niz, José María
Turica, Mariana Daniela
Pedarros, Analía Soledad
Hopp, Horacio Esteban
Lewi, Dalia Marcela
author Salvador, Ricardo
author_facet Salvador, Ricardo
Maskin, Laura
Niz, José María
Turica, Mariana Daniela
Pedarros, Analía Soledad
Hopp, Horacio Esteban
Lewi, Dalia Marcela
author_role author
author2 Maskin, Laura
Niz, José María
Turica, Mariana Daniela
Pedarros, Analía Soledad
Hopp, Horacio Esteban
Lewi, Dalia Marcela
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Gossypium hirsutum
Algodón
Cotton
Biological Control
Pest Control
Control Biológico
Control de Plagas
Transgenic Cotton Plants
Virus Induced Gene Silencing
RNA Interference
topic Gossypium hirsutum
Algodón
Cotton
Biological Control
Pest Control
Control Biológico
Control de Plagas
Transgenic Cotton Plants
Virus Induced Gene Silencing
RNA Interference
dc.description.none.fl_txt_mv Cultivated cotton (Gossypium hirsutum) is heavily attacked by various species of insects worldwide and breeding of new varieties resistant to pests is still a hard battle to win. RNAi technology is an important reverse genetics tool to induce gene silencing in eukaryotic organisms and produce phenotypic modifications. In cotton, RNAi was applied to investigate gene function and enhance resistance to insects and pathogens. Different methods and techniques can be used to synthetize double stranded RNA (dsRNA) into plant cells. The Agrobacterium-mediated transformation is a common method to introduce RNAi binary plasmids into cotton genome and obtain stable transgenics plants. This methodology includes the coculture of cotton tissues with Agrobacterium cultures, selection of transgenic cells and induction of somatic embryogenesis to finally obtain transgenic plants after a relatively long period of time. The transient synthesis of dsRNA mediated by virus-induced gene silencing (VIGS) in cotton is an alternative to anticipate the silencing effect of a specific RNA sequence, prior to the development of a stable transgenic plant. VIGS vectors are incorporated into the plant by agroinfiltration technique. During VIGS replication inside plant cells, synthetized dsRNA allows the study on specific heterologous gene expression including the phenotypic effect on herbivorous target pests, thus facilitating a rapid evaluation of dsRNA expressed in cotton plants against individual insect target genes. Here we describe the complementation of these two techniques to evaluate RNAi-based cotton plant protection against insect pests.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Maskin, Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Niz, José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Turica, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Pedarros, Analía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Agrobiotecnología; Argentina
Fil: Lewi, Dalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
description Cultivated cotton (Gossypium hirsutum) is heavily attacked by various species of insects worldwide and breeding of new varieties resistant to pests is still a hard battle to win. RNAi technology is an important reverse genetics tool to induce gene silencing in eukaryotic organisms and produce phenotypic modifications. In cotton, RNAi was applied to investigate gene function and enhance resistance to insects and pathogens. Different methods and techniques can be used to synthetize double stranded RNA (dsRNA) into plant cells. The Agrobacterium-mediated transformation is a common method to introduce RNAi binary plasmids into cotton genome and obtain stable transgenics plants. This methodology includes the coculture of cotton tissues with Agrobacterium cultures, selection of transgenic cells and induction of somatic embryogenesis to finally obtain transgenic plants after a relatively long period of time. The transient synthesis of dsRNA mediated by virus-induced gene silencing (VIGS) in cotton is an alternative to anticipate the silencing effect of a specific RNA sequence, prior to the development of a stable transgenic plant. VIGS vectors are incorporated into the plant by agroinfiltration technique. During VIGS replication inside plant cells, synthetized dsRNA allows the study on specific heterologous gene expression including the phenotypic effect on herbivorous target pests, thus facilitating a rapid evaluation of dsRNA expressed in cotton plants against individual insect target genes. Here we describe the complementation of these two techniques to evaluate RNAi-based cotton plant protection against insect pests.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-26T22:43:52Z
2022-07-26T22:43:52Z
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/bookPart
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_3248
info:ar-repo/semantics/parteDeLibro
format bookPart
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/12411
https://link.springer.com/protocol/10.1007/978-1-0716-1633-8_17
Salvador, R. et al. (2022). RNAi Expression in Cotton for Control of Herbivorous Insects. pp 217-233. In: Vaschetto, L.M. (eds) RNAi Strategies for Pest Management. Methods in Molecular Biology, vol 2360. Humana, New York, NY
978-1-0716-1633-8
https://doi.org/10.1007/978-1-0716-1633-8_17
url http://hdl.handle.net/20.500.12123/12411
https://link.springer.com/protocol/10.1007/978-1-0716-1633-8_17
https://doi.org/10.1007/978-1-0716-1633-8_17
identifier_str_mv Salvador, R. et al. (2022). RNAi Expression in Cotton for Control of Herbivorous Insects. pp 217-233. In: Vaschetto, L.M. (eds) RNAi Strategies for Pest Management. Methods in Molecular Biology, vol 2360. Humana, New York, NY
978-1-0716-1633-8
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Methods in Molecular Biology (Springer)
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv RNAi Strategies for Pest Management : methods and protocols / editors: Luis María Vaschetto. New York: Humana, 2021. p. 217–233
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
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instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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