Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae)

Autores
Salvador, Ricardo; Perez Principi, Ricardo Dario; Berretta, Marcelo Facundo; Fernandez, Paula Del Carmen; Paniego, Norma Beatriz; Sciocco, Alicia Ines; Hopp, Horacio Esteban
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Anthonomus grandis Boheman is a key pest in cotton crops in the New World. Its larval stage develops within the flower bud using it as food and as protection against its predators. This behavior limits the effectiveness of its control using conventional insecticide applications and biocontrol techniques. In spite of its importance, little is known about its genome sequence and, more important, its specific expression in key organs like the midgut. Total mRNA isolated from larval midguts was used for pyrosequencing. Sequence reads were assembled and annotated to generate a unigene data set. In total, 400,000 reads from A. grandis midgut with an average length of 237 bp were assembled and combined into 20,915 contigs. The assembled reads fell into 6,621 genes models. BlastX search using the NCBI-NR database showed that 3,006 unigenes had significant matches to known sequences. Gene Ontology (GO) mapping analysis evidenced that A. grandis is able to transcripts coding for proteins involved in catalytic processing of macromolecules that allows its adaptation to very different feeding source scenarios. Furthermore, transcripts encoding for proteins involved in detoxification mechanisms such as p450 genes, glutathione-S-transferase, and carboxylesterases are also expressed. This is the first report of a transcriptomic study in A. grandis and the largest set of sequence data reported for this species. These data are valuable resources to expand the knowledge of this insect group and could be used in the design of new control strategies based in molecular information.
Instituto de Microbiología y Zoología Agrícola
Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Perez Principi, Ricardo Dario. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Journal of Insect Science 14 (1) : 219. (2014)
Materia
Coleoptera
Anthonomus grandis
Plagas de Plantas
Control de Plagas
Algodón
ARN
Pests of Plants
Pest Control
Cotton
RNA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5422
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5422
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae)Salvador, RicardoPerez Principi, Ricardo DarioBerretta, Marcelo FacundoFernandez, Paula Del CarmenPaniego, Norma BeatrizSciocco, Alicia InesHopp, Horacio EstebanColeopteraAnthonomus grandisPlagas de PlantasControl de PlagasAlgodónARNPests of PlantsPest ControlCottonRNAAnthonomus grandis Boheman is a key pest in cotton crops in the New World. Its larval stage develops within the flower bud using it as food and as protection against its predators. This behavior limits the effectiveness of its control using conventional insecticide applications and biocontrol techniques. In spite of its importance, little is known about its genome sequence and, more important, its specific expression in key organs like the midgut. Total mRNA isolated from larval midguts was used for pyrosequencing. Sequence reads were assembled and annotated to generate a unigene data set. In total, 400,000 reads from A. grandis midgut with an average length of 237 bp were assembled and combined into 20,915 contigs. The assembled reads fell into 6,621 genes models. BlastX search using the NCBI-NR database showed that 3,006 unigenes had significant matches to known sequences. Gene Ontology (GO) mapping analysis evidenced that A. grandis is able to transcripts coding for proteins involved in catalytic processing of macromolecules that allows its adaptation to very different feeding source scenarios. Furthermore, transcripts encoding for proteins involved in detoxification mechanisms such as p450 genes, glutathione-S-transferase, and carboxylesterases are also expressed. This is the first report of a transcriptomic study in A. grandis and the largest set of sequence data reported for this species. These data are valuable resources to expand the knowledge of this insect group and could be used in the design of new control strategies based in molecular information.Instituto de Microbiología y Zoología AgrícolaFil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Perez Principi, Ricardo Dario. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaEntomological Society of America2019-07-03T12:37:10Z2019-07-03T12:37:10Z2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://academic.oup.com/jinsectscience/article/14/1/219/2381789http://hdl.handle.net/20.500.12123/54221536-2442https://doi.org/10.1093/jisesa/ieu081Journal of Insect Science 14 (1) : 219. (2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:48:03Zoai:localhost:20.500.12123/5422instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:06.099INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae)
title Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae)
spellingShingle Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae)
Salvador, Ricardo
Coleoptera
Anthonomus grandis
Plagas de Plantas
Control de Plagas
Algodón
ARN
Pests of Plants
Pest Control
Cotton
RNA
title_short Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae)
title_full Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae)
title_fullStr Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae)
title_full_unstemmed Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae)
title_sort Transcriptomic Survey of the Midgut of Anthonomus grandis (Coleoptera: Curculionidae)
dc.creator.none.fl_str_mv Salvador, Ricardo
Perez Principi, Ricardo Dario
Berretta, Marcelo Facundo
Fernandez, Paula Del Carmen
Paniego, Norma Beatriz
Sciocco, Alicia Ines
Hopp, Horacio Esteban
author Salvador, Ricardo
author_facet Salvador, Ricardo
Perez Principi, Ricardo Dario
Berretta, Marcelo Facundo
Fernandez, Paula Del Carmen
Paniego, Norma Beatriz
Sciocco, Alicia Ines
Hopp, Horacio Esteban
author_role author
author2 Perez Principi, Ricardo Dario
Berretta, Marcelo Facundo
Fernandez, Paula Del Carmen
Paniego, Norma Beatriz
Sciocco, Alicia Ines
Hopp, Horacio Esteban
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Coleoptera
Anthonomus grandis
Plagas de Plantas
Control de Plagas
Algodón
ARN
Pests of Plants
Pest Control
Cotton
RNA
topic Coleoptera
Anthonomus grandis
Plagas de Plantas
Control de Plagas
Algodón
ARN
Pests of Plants
Pest Control
Cotton
RNA
dc.description.none.fl_txt_mv Anthonomus grandis Boheman is a key pest in cotton crops in the New World. Its larval stage develops within the flower bud using it as food and as protection against its predators. This behavior limits the effectiveness of its control using conventional insecticide applications and biocontrol techniques. In spite of its importance, little is known about its genome sequence and, more important, its specific expression in key organs like the midgut. Total mRNA isolated from larval midguts was used for pyrosequencing. Sequence reads were assembled and annotated to generate a unigene data set. In total, 400,000 reads from A. grandis midgut with an average length of 237 bp were assembled and combined into 20,915 contigs. The assembled reads fell into 6,621 genes models. BlastX search using the NCBI-NR database showed that 3,006 unigenes had significant matches to known sequences. Gene Ontology (GO) mapping analysis evidenced that A. grandis is able to transcripts coding for proteins involved in catalytic processing of macromolecules that allows its adaptation to very different feeding source scenarios. Furthermore, transcripts encoding for proteins involved in detoxification mechanisms such as p450 genes, glutathione-S-transferase, and carboxylesterases are also expressed. This is the first report of a transcriptomic study in A. grandis and the largest set of sequence data reported for this species. These data are valuable resources to expand the knowledge of this insect group and could be used in the design of new control strategies based in molecular information.
Instituto de Microbiología y Zoología Agrícola
Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Perez Principi, Ricardo Dario. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description Anthonomus grandis Boheman is a key pest in cotton crops in the New World. Its larval stage develops within the flower bud using it as food and as protection against its predators. This behavior limits the effectiveness of its control using conventional insecticide applications and biocontrol techniques. In spite of its importance, little is known about its genome sequence and, more important, its specific expression in key organs like the midgut. Total mRNA isolated from larval midguts was used for pyrosequencing. Sequence reads were assembled and annotated to generate a unigene data set. In total, 400,000 reads from A. grandis midgut with an average length of 237 bp were assembled and combined into 20,915 contigs. The assembled reads fell into 6,621 genes models. BlastX search using the NCBI-NR database showed that 3,006 unigenes had significant matches to known sequences. Gene Ontology (GO) mapping analysis evidenced that A. grandis is able to transcripts coding for proteins involved in catalytic processing of macromolecules that allows its adaptation to very different feeding source scenarios. Furthermore, transcripts encoding for proteins involved in detoxification mechanisms such as p450 genes, glutathione-S-transferase, and carboxylesterases are also expressed. This is the first report of a transcriptomic study in A. grandis and the largest set of sequence data reported for this species. These data are valuable resources to expand the knowledge of this insect group and could be used in the design of new control strategies based in molecular information.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
2019-07-03T12:37:10Z
2019-07-03T12:37:10Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://academic.oup.com/jinsectscience/article/14/1/219/2381789
http://hdl.handle.net/20.500.12123/5422
1536-2442
https://doi.org/10.1093/jisesa/ieu081
url https://academic.oup.com/jinsectscience/article/14/1/219/2381789
http://hdl.handle.net/20.500.12123/5422
https://doi.org/10.1093/jisesa/ieu081
identifier_str_mv 1536-2442
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Entomological Society of America
publisher.none.fl_str_mv Entomological Society of America
dc.source.none.fl_str_mv Journal of Insect Science 14 (1) : 219. (2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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