Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus

Autores
Delfosse, Veronica Cecilia; Debat, Humberto Julio; Zavallo, Diego; Alvarez, Liliana; Gomez Talquenca, Gonzalo; Bonacic Kresic, Iván; Asurmendi, Sebastian; Distefano, Ana Julia
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cotton is a commercial crop of global importance whose versatile fibre is widely used in the textile industry. Cotton is vulnerable to infectious pathogens, including viruses, that are a major threat to cotton production. Cotton blue disease is caused by cotton leafroll dwarf virus (CLRDV), a polerovirus transmitted by aphids. This virus is controlled by sowing cotton varieties resistant to CLRDV, although, in recent years an atypical variant (CLRDV-at), that breaks the resistance, has been identified. An outbreak of a disease occurred in cotton fields of the province of Chaco, in the north-west of Argentina, in the 2014–2015 growing season. NuOpal cotton plants (a variety resistant to CLRDV and susceptible to CLRDV-at) showed mosaic symptoms and had virus-transmitting insect vectors, such as aphids and whiteflies (Bemisia tabaci). The symptoms were common for virus infections but different from those caused by CLRDV or CLRDV-at. According to a reverse transcription-PCR analysis with polerovirus primers and sequencing, the plants contained CLRDV-at. To identify the complete virome of these plants, we performed a massive RNA-based deep sequencing assay using Illumina technology. As a result, a new begomovirus that infects cotton in Argentina was identified. Structural and functional annotation indicated that its sequences corresponded to complete DNA components A and B of a novel New World bipartite begomovirus. Genetic distance and evolutionary analyses support that the detected sequences correspond to a new virus, which we propose to name cotton mosaic virus (CoMV). In addition, we report the first begomovirus and polerovirus coinfection in cotton.
Instituto de Biotecnología
Fil: Delfosse, Veronica Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Delfosse, Veronica Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Delfosse, Veronica Cecilia. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Debat, Humberto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Zavallo, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarez, Liliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Alvarez, Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gomez Talquenca, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Bonacic Kresic, Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Sáenz Peña; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Distefano, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Distefano, Ana Julia. Universidad Nacional de Luján; Argentina
Fuente
Plant Pathology 00 : 1–11 (First published: 22 May 2024)
Materia
Begomovirus
Cotton
Algodón
Gossypium
Polerovirus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/18293
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oai_identifier_str oai:localhost:20.500.12123/18293
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network_name_str INTA Digital (INTA)
spelling Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirusDelfosse, Veronica CeciliaDebat, Humberto JulioZavallo, DiegoAlvarez, LilianaGomez Talquenca, GonzaloBonacic Kresic, IvánAsurmendi, SebastianDistefano, Ana JuliaBegomovirusCottonAlgodónGossypiumPolerovirusCotton is a commercial crop of global importance whose versatile fibre is widely used in the textile industry. Cotton is vulnerable to infectious pathogens, including viruses, that are a major threat to cotton production. Cotton blue disease is caused by cotton leafroll dwarf virus (CLRDV), a polerovirus transmitted by aphids. This virus is controlled by sowing cotton varieties resistant to CLRDV, although, in recent years an atypical variant (CLRDV-at), that breaks the resistance, has been identified. An outbreak of a disease occurred in cotton fields of the province of Chaco, in the north-west of Argentina, in the 2014–2015 growing season. NuOpal cotton plants (a variety resistant to CLRDV and susceptible to CLRDV-at) showed mosaic symptoms and had virus-transmitting insect vectors, such as aphids and whiteflies (Bemisia tabaci). The symptoms were common for virus infections but different from those caused by CLRDV or CLRDV-at. According to a reverse transcription-PCR analysis with polerovirus primers and sequencing, the plants contained CLRDV-at. To identify the complete virome of these plants, we performed a massive RNA-based deep sequencing assay using Illumina technology. As a result, a new begomovirus that infects cotton in Argentina was identified. Structural and functional annotation indicated that its sequences corresponded to complete DNA components A and B of a novel New World bipartite begomovirus. Genetic distance and evolutionary analyses support that the detected sequences correspond to a new virus, which we propose to name cotton mosaic virus (CoMV). In addition, we report the first begomovirus and polerovirus coinfection in cotton.Instituto de BiotecnologíaFil: Delfosse, Veronica Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Delfosse, Veronica Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Delfosse, Veronica Cecilia. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Debat, Humberto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Zavallo, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alvarez, Liliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Alvarez, Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gomez Talquenca, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Bonacic Kresic, Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Sáenz Peña; ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Distefano, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Distefano, Ana Julia. Universidad Nacional de Luján; ArgentinaWiley2024-06-28T10:01:38Z2024-06-28T10:01:38Z2024-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/18293https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.139341365-3059https://doi.org/10.1111/ppa.13934Plant Pathology 00 : 1–11 (First published: 22 May 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:37Zoai:localhost:20.500.12123/18293instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:37.824INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus
title Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus
spellingShingle Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus
Delfosse, Veronica Cecilia
Begomovirus
Cotton
Algodón
Gossypium
Polerovirus
title_short Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus
title_full Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus
title_fullStr Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus
title_full_unstemmed Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus
title_sort Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus
dc.creator.none.fl_str_mv Delfosse, Veronica Cecilia
Debat, Humberto Julio
Zavallo, Diego
Alvarez, Liliana
Gomez Talquenca, Gonzalo
Bonacic Kresic, Iván
Asurmendi, Sebastian
Distefano, Ana Julia
author Delfosse, Veronica Cecilia
author_facet Delfosse, Veronica Cecilia
Debat, Humberto Julio
Zavallo, Diego
Alvarez, Liliana
Gomez Talquenca, Gonzalo
Bonacic Kresic, Iván
Asurmendi, Sebastian
Distefano, Ana Julia
author_role author
author2 Debat, Humberto Julio
Zavallo, Diego
Alvarez, Liliana
Gomez Talquenca, Gonzalo
Bonacic Kresic, Iván
Asurmendi, Sebastian
Distefano, Ana Julia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Begomovirus
Cotton
Algodón
Gossypium
Polerovirus
topic Begomovirus
Cotton
Algodón
Gossypium
Polerovirus
dc.description.none.fl_txt_mv Cotton is a commercial crop of global importance whose versatile fibre is widely used in the textile industry. Cotton is vulnerable to infectious pathogens, including viruses, that are a major threat to cotton production. Cotton blue disease is caused by cotton leafroll dwarf virus (CLRDV), a polerovirus transmitted by aphids. This virus is controlled by sowing cotton varieties resistant to CLRDV, although, in recent years an atypical variant (CLRDV-at), that breaks the resistance, has been identified. An outbreak of a disease occurred in cotton fields of the province of Chaco, in the north-west of Argentina, in the 2014–2015 growing season. NuOpal cotton plants (a variety resistant to CLRDV and susceptible to CLRDV-at) showed mosaic symptoms and had virus-transmitting insect vectors, such as aphids and whiteflies (Bemisia tabaci). The symptoms were common for virus infections but different from those caused by CLRDV or CLRDV-at. According to a reverse transcription-PCR analysis with polerovirus primers and sequencing, the plants contained CLRDV-at. To identify the complete virome of these plants, we performed a massive RNA-based deep sequencing assay using Illumina technology. As a result, a new begomovirus that infects cotton in Argentina was identified. Structural and functional annotation indicated that its sequences corresponded to complete DNA components A and B of a novel New World bipartite begomovirus. Genetic distance and evolutionary analyses support that the detected sequences correspond to a new virus, which we propose to name cotton mosaic virus (CoMV). In addition, we report the first begomovirus and polerovirus coinfection in cotton.
Instituto de Biotecnología
Fil: Delfosse, Veronica Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Delfosse, Veronica Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Delfosse, Veronica Cecilia. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Debat, Humberto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Zavallo, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarez, Liliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Alvarez, Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gomez Talquenca, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Bonacic Kresic, Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Sáenz Peña; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Distefano, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Distefano, Ana Julia. Universidad Nacional de Luján; Argentina
description Cotton is a commercial crop of global importance whose versatile fibre is widely used in the textile industry. Cotton is vulnerable to infectious pathogens, including viruses, that are a major threat to cotton production. Cotton blue disease is caused by cotton leafroll dwarf virus (CLRDV), a polerovirus transmitted by aphids. This virus is controlled by sowing cotton varieties resistant to CLRDV, although, in recent years an atypical variant (CLRDV-at), that breaks the resistance, has been identified. An outbreak of a disease occurred in cotton fields of the province of Chaco, in the north-west of Argentina, in the 2014–2015 growing season. NuOpal cotton plants (a variety resistant to CLRDV and susceptible to CLRDV-at) showed mosaic symptoms and had virus-transmitting insect vectors, such as aphids and whiteflies (Bemisia tabaci). The symptoms were common for virus infections but different from those caused by CLRDV or CLRDV-at. According to a reverse transcription-PCR analysis with polerovirus primers and sequencing, the plants contained CLRDV-at. To identify the complete virome of these plants, we performed a massive RNA-based deep sequencing assay using Illumina technology. As a result, a new begomovirus that infects cotton in Argentina was identified. Structural and functional annotation indicated that its sequences corresponded to complete DNA components A and B of a novel New World bipartite begomovirus. Genetic distance and evolutionary analyses support that the detected sequences correspond to a new virus, which we propose to name cotton mosaic virus (CoMV). In addition, we report the first begomovirus and polerovirus coinfection in cotton.
publishDate 2024
dc.date.none.fl_str_mv 2024-06-28T10:01:38Z
2024-06-28T10:01:38Z
2024-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/18293
https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13934
1365-3059
https://doi.org/10.1111/ppa.13934
url http://hdl.handle.net/20.500.12123/18293
https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13934
https://doi.org/10.1111/ppa.13934
identifier_str_mv 1365-3059
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Plant Pathology 00 : 1–11 (First published: 22 May 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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