Shiga toxin-producing Escherichia coli (STEC) O22:H8 isolated from cattle reduces E. coli O157:H7 adherence in vitro and in vivo

Autores
Martorelli, Luisina; Albanese, Adriana Andrea; Vilte, Daniel Alejandro; Cantet, Rodolfo Juan Carlos; Bentancor, Adriana Beatriz; Zolezzi, Gisela; Chinen, Isabel; Ibarra, Cristina E.; Rivas, Marta; Mercado, Elsa Cristina; Cataldi, Angel Adrian
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Problem addressed: Shiga toxin-producing Escherichia coli (STEC) are a group of bacteria responsible for foodassociated diseases. Clinical features include a wide range of symptoms such as diarrhea, hemorrhagic colitis and the hemolytic uremic syndrome (HUS), a life-threatening condition. Objective: Our group has observed that animals naturally colonized with STEC strains of unknown serotype were not efficiently colonized with E. coli O157:H7 after experimental infection. In order to assess the basis of the interference, three STEC strains were isolated from STEC persistently-colonized healthy cattle from a dairy farm in Buenos Aires, Argentina. Methods and results: The three isolated strains are E. coli O22:H8 and carry the stx1 and stx2d genes. The activatable activity of Stx2d was demonstrated in vitro. The three strains carry the adhesins iha, ehaA and lpfO113. E. coli O22:H8 formed stronger biofilms in abiotic surface than E. coli O157:H7 (eae+, stx2+) and displayed a more adherent phenotype in vitro towards HeLa cells. Furthermore, when both serotypes were cultured together O22:H8 could reduce O157:H7 adherence in vitro. When calves were intragastrically pre-challenged with 108 CFU of a mixture of the three STEC strains and two days later challenged with the same dose of the strain E. coli O157:H7 438/99, the shedding of the pathogen was significantly reduced. Conclusions: These results suggest that E. coli O22:H8, a serotype rarely associated with human illness, might compete with O157:H7 at the bovine recto-anal junction, making non-O157 carrying-calves less susceptible to O157:H7 colonization and shedding of the bacteria to the environment.
Inst. de Patobiología
Fil: Martorelli, Luisina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Albanese, Adriana Andrea. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Cantet, Rodolfo Juan Carlos. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bentancor, Adriana B. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Microbiología; Argentina
Fil: Zolezzi, Gisela. INEI-ANLIS «Dr. Carlos G. Malbrán». Departamento Bacteriología. Servicio Fisiopatogenia; Argentina
Fil: Chinen, Isabel. INEI-ANLIS «Dr. Carlos G. Malbrán». Departamento Bacteriología. Servicio Fisiopatogenia; Argentina
Fil: Ibarra, Cristina E. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Rivas, Marta. INEI-ANLIS «Dr. Carlos G. Malbrán». Departamento Bacteriología. Servicio Fisiopatogenia; Argentina
Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Veterinary microbiology 208 : 8-17. (September 2017)
Materia
Enfermedades de los Animales
Escherichia Coli
Ganado Bovino
Experimentación In Vitro
Experimentación In Vivo
Animal Disesase
Cattle
In Vitro Experimentation
In Vivo Experimentation
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1487
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1487
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Shiga toxin-producing Escherichia coli (STEC) O22:H8 isolated from cattle reduces E. coli O157:H7 adherence in vitro and in vivoMartorelli, LuisinaAlbanese, Adriana AndreaVilte, Daniel AlejandroCantet, Rodolfo Juan CarlosBentancor, Adriana BeatrizZolezzi, GiselaChinen, IsabelIbarra, Cristina E.Rivas, MartaMercado, Elsa CristinaCataldi, Angel AdrianEnfermedades de los AnimalesEscherichia ColiGanado BovinoExperimentación In VitroExperimentación In VivoAnimal DisesaseCattleIn Vitro ExperimentationIn Vivo ExperimentationProblem addressed: Shiga toxin-producing Escherichia coli (STEC) are a group of bacteria responsible for foodassociated diseases. Clinical features include a wide range of symptoms such as diarrhea, hemorrhagic colitis and the hemolytic uremic syndrome (HUS), a life-threatening condition. Objective: Our group has observed that animals naturally colonized with STEC strains of unknown serotype were not efficiently colonized with E. coli O157:H7 after experimental infection. In order to assess the basis of the interference, three STEC strains were isolated from STEC persistently-colonized healthy cattle from a dairy farm in Buenos Aires, Argentina. Methods and results: The three isolated strains are E. coli O22:H8 and carry the stx1 and stx2d genes. The activatable activity of Stx2d was demonstrated in vitro. The three strains carry the adhesins iha, ehaA and lpfO113. E. coli O22:H8 formed stronger biofilms in abiotic surface than E. coli O157:H7 (eae+, stx2+) and displayed a more adherent phenotype in vitro towards HeLa cells. Furthermore, when both serotypes were cultured together O22:H8 could reduce O157:H7 adherence in vitro. When calves were intragastrically pre-challenged with 108 CFU of a mixture of the three STEC strains and two days later challenged with the same dose of the strain E. coli O157:H7 438/99, the shedding of the pathogen was significantly reduced. Conclusions: These results suggest that E. coli O22:H8, a serotype rarely associated with human illness, might compete with O157:H7 at the bovine recto-anal junction, making non-O157 carrying-calves less susceptible to O157:H7 colonization and shedding of the bacteria to the environment.Inst. de PatobiologíaFil: Martorelli, Luisina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Albanese, Adriana Andrea. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Cantet, Rodolfo Juan Carlos. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bentancor, Adriana B. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Microbiología; ArgentinaFil: Zolezzi, Gisela. INEI-ANLIS «Dr. Carlos G. Malbrán». Departamento Bacteriología. Servicio Fisiopatogenia; ArgentinaFil: Chinen, Isabel. INEI-ANLIS «Dr. Carlos G. Malbrán». Departamento Bacteriología. Servicio Fisiopatogenia; ArgentinaFil: Ibarra, Cristina E. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Rivas, Marta. INEI-ANLIS «Dr. Carlos G. Malbrán». Departamento Bacteriología. Servicio Fisiopatogenia; ArgentinaFil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina2017-10-13T14:09:43Z2017-10-13T14:09:43Z2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1487http://www.sciencedirect.com/science/article/pii/S03781135173064660378-1135 (Print)1873-2542 (Online)https://doi.org/10.1016/j.vetmic.2017.06.021Veterinary microbiology 208 : 8-17. (September 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:06Zoai:localhost:20.500.12123/1487instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:06.466INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Shiga toxin-producing Escherichia coli (STEC) O22:H8 isolated from cattle reduces E. coli O157:H7 adherence in vitro and in vivo
title Shiga toxin-producing Escherichia coli (STEC) O22:H8 isolated from cattle reduces E. coli O157:H7 adherence in vitro and in vivo
spellingShingle Shiga toxin-producing Escherichia coli (STEC) O22:H8 isolated from cattle reduces E. coli O157:H7 adherence in vitro and in vivo
Martorelli, Luisina
Enfermedades de los Animales
Escherichia Coli
Ganado Bovino
Experimentación In Vitro
Experimentación In Vivo
Animal Disesase
Cattle
In Vitro Experimentation
In Vivo Experimentation
title_short Shiga toxin-producing Escherichia coli (STEC) O22:H8 isolated from cattle reduces E. coli O157:H7 adherence in vitro and in vivo
title_full Shiga toxin-producing Escherichia coli (STEC) O22:H8 isolated from cattle reduces E. coli O157:H7 adherence in vitro and in vivo
title_fullStr Shiga toxin-producing Escherichia coli (STEC) O22:H8 isolated from cattle reduces E. coli O157:H7 adherence in vitro and in vivo
title_full_unstemmed Shiga toxin-producing Escherichia coli (STEC) O22:H8 isolated from cattle reduces E. coli O157:H7 adherence in vitro and in vivo
title_sort Shiga toxin-producing Escherichia coli (STEC) O22:H8 isolated from cattle reduces E. coli O157:H7 adherence in vitro and in vivo
dc.creator.none.fl_str_mv Martorelli, Luisina
Albanese, Adriana Andrea
Vilte, Daniel Alejandro
Cantet, Rodolfo Juan Carlos
Bentancor, Adriana Beatriz
Zolezzi, Gisela
Chinen, Isabel
Ibarra, Cristina E.
Rivas, Marta
Mercado, Elsa Cristina
Cataldi, Angel Adrian
author Martorelli, Luisina
author_facet Martorelli, Luisina
Albanese, Adriana Andrea
Vilte, Daniel Alejandro
Cantet, Rodolfo Juan Carlos
Bentancor, Adriana Beatriz
Zolezzi, Gisela
Chinen, Isabel
Ibarra, Cristina E.
Rivas, Marta
Mercado, Elsa Cristina
Cataldi, Angel Adrian
author_role author
author2 Albanese, Adriana Andrea
Vilte, Daniel Alejandro
Cantet, Rodolfo Juan Carlos
Bentancor, Adriana Beatriz
Zolezzi, Gisela
Chinen, Isabel
Ibarra, Cristina E.
Rivas, Marta
Mercado, Elsa Cristina
Cataldi, Angel Adrian
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Escherichia Coli
Ganado Bovino
Experimentación In Vitro
Experimentación In Vivo
Animal Disesase
Cattle
In Vitro Experimentation
In Vivo Experimentation
topic Enfermedades de los Animales
Escherichia Coli
Ganado Bovino
Experimentación In Vitro
Experimentación In Vivo
Animal Disesase
Cattle
In Vitro Experimentation
In Vivo Experimentation
dc.description.none.fl_txt_mv Problem addressed: Shiga toxin-producing Escherichia coli (STEC) are a group of bacteria responsible for foodassociated diseases. Clinical features include a wide range of symptoms such as diarrhea, hemorrhagic colitis and the hemolytic uremic syndrome (HUS), a life-threatening condition. Objective: Our group has observed that animals naturally colonized with STEC strains of unknown serotype were not efficiently colonized with E. coli O157:H7 after experimental infection. In order to assess the basis of the interference, three STEC strains were isolated from STEC persistently-colonized healthy cattle from a dairy farm in Buenos Aires, Argentina. Methods and results: The three isolated strains are E. coli O22:H8 and carry the stx1 and stx2d genes. The activatable activity of Stx2d was demonstrated in vitro. The three strains carry the adhesins iha, ehaA and lpfO113. E. coli O22:H8 formed stronger biofilms in abiotic surface than E. coli O157:H7 (eae+, stx2+) and displayed a more adherent phenotype in vitro towards HeLa cells. Furthermore, when both serotypes were cultured together O22:H8 could reduce O157:H7 adherence in vitro. When calves were intragastrically pre-challenged with 108 CFU of a mixture of the three STEC strains and two days later challenged with the same dose of the strain E. coli O157:H7 438/99, the shedding of the pathogen was significantly reduced. Conclusions: These results suggest that E. coli O22:H8, a serotype rarely associated with human illness, might compete with O157:H7 at the bovine recto-anal junction, making non-O157 carrying-calves less susceptible to O157:H7 colonization and shedding of the bacteria to the environment.
Inst. de Patobiología
Fil: Martorelli, Luisina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Albanese, Adriana Andrea. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Cantet, Rodolfo Juan Carlos. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bentancor, Adriana B. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Microbiología; Argentina
Fil: Zolezzi, Gisela. INEI-ANLIS «Dr. Carlos G. Malbrán». Departamento Bacteriología. Servicio Fisiopatogenia; Argentina
Fil: Chinen, Isabel. INEI-ANLIS «Dr. Carlos G. Malbrán». Departamento Bacteriología. Servicio Fisiopatogenia; Argentina
Fil: Ibarra, Cristina E. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Rivas, Marta. INEI-ANLIS «Dr. Carlos G. Malbrán». Departamento Bacteriología. Servicio Fisiopatogenia; Argentina
Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description Problem addressed: Shiga toxin-producing Escherichia coli (STEC) are a group of bacteria responsible for foodassociated diseases. Clinical features include a wide range of symptoms such as diarrhea, hemorrhagic colitis and the hemolytic uremic syndrome (HUS), a life-threatening condition. Objective: Our group has observed that animals naturally colonized with STEC strains of unknown serotype were not efficiently colonized with E. coli O157:H7 after experimental infection. In order to assess the basis of the interference, three STEC strains were isolated from STEC persistently-colonized healthy cattle from a dairy farm in Buenos Aires, Argentina. Methods and results: The three isolated strains are E. coli O22:H8 and carry the stx1 and stx2d genes. The activatable activity of Stx2d was demonstrated in vitro. The three strains carry the adhesins iha, ehaA and lpfO113. E. coli O22:H8 formed stronger biofilms in abiotic surface than E. coli O157:H7 (eae+, stx2+) and displayed a more adherent phenotype in vitro towards HeLa cells. Furthermore, when both serotypes were cultured together O22:H8 could reduce O157:H7 adherence in vitro. When calves were intragastrically pre-challenged with 108 CFU of a mixture of the three STEC strains and two days later challenged with the same dose of the strain E. coli O157:H7 438/99, the shedding of the pathogen was significantly reduced. Conclusions: These results suggest that E. coli O22:H8, a serotype rarely associated with human illness, might compete with O157:H7 at the bovine recto-anal junction, making non-O157 carrying-calves less susceptible to O157:H7 colonization and shedding of the bacteria to the environment.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-13T14:09:43Z
2017-10-13T14:09:43Z
2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
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status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1487
http://www.sciencedirect.com/science/article/pii/S0378113517306466
0378-1135 (Print)
1873-2542 (Online)
https://doi.org/10.1016/j.vetmic.2017.06.021
url http://hdl.handle.net/20.500.12123/1487
http://www.sciencedirect.com/science/article/pii/S0378113517306466
https://doi.org/10.1016/j.vetmic.2017.06.021
identifier_str_mv 0378-1135 (Print)
1873-2542 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
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dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Veterinary microbiology 208 : 8-17. (September 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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