Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques

Autores
Forcada, Fernando; Buffoni, Andres; Abecia, José Alfonso; Asenjo, B.; Palacin, José Ignacio; Vázquez, M.I.; Rodriguez Castillo, José del Carmen; Sanchez Prieto, L.; Casao, A.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study investigated the use of previously superovulated ovaries as a source of oocytes, assessing the competence of them for in vitro embryo production. Two superovulatory treatments were performed: equine Chorionic Gonadotrophin (eCG) plus porcine Follicle-Stimulating Hormone (pFSH) in a single dose or the conventional protocol of six decreasing doses of pFSH. Thirty donor ewes of the endangered Ojalada breed were given either the simplified (group S; n=15) or the decreasing-dose (group D; n=15) treatments three times at intervals of ≥50 days. Ovaries were recovered on day 7 after the oestrus following the third treatment, just after embryo flushing, and the oocytes were collected to assess in vitro maturation, fertilisation and development to the blastocyst stage. The two superovulatory treatments did not differ in the mean number of oocytes selected for maturation (7.1±1.2 and 8.5±1.5 per ewe in the D and S groups, respectively). The oocytes recovered from ewes in Group D (87.5%) had a significantly (p<0.05) higher maturation rate than did those recovered from ewes in group S (75%), but no differences were found in fertilisation rate (94% and 94.6% in the D and S groups, respectively); both groups did not differ in their blastocyst rates and the total number of cells in in vitro-produced blastocysts. In the two experimental groups, 1.7 (D) and 1.8 (S) in vitro-produced blastocysts were generated per ewe, which indicate that it is feasible to combine in vivo and in vitro techniques to maximise embryo production in endangered sheep breeds.
EEA Chubut
Fil: Forcada, Fernando. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España
Fil: Buffoni, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chubut; Argentina
Fil: Abecia, José Alfonso. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España
Fil: Asenjo, B. Universidad de Valladolid. Escuela Universitaria de Ingenierías Agrarias de Soria; España
Fil: Palacin, José Ignacio. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España
Fil: Vázquez, M.I. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España
Fil: Rodriguez Castillo, José del Carmen. Benemérita Universidad Autónoma de Puebla. Facultad de Medicina Veterinaria y Zootecnia; México
Fil: Sanchez Prieto, L. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España
Fil: Casao, A. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España
Fuente
Journal of Applied Animal Research 39 (4) : 412-417 (2011)
Materia
Ovinos
Razas (animales)
Conservación de Embriones
Experimentación in Vitro
Experimentación in Vivo
Superovulación
Sheep
Breeds (animals)
Embryo Preservation
In Vitro Experimentation
In Vivo Experimentation
Superovulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5073
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5073
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniquesForcada, FernandoBuffoni, AndresAbecia, José AlfonsoAsenjo, B.Palacin, José IgnacioVázquez, M.I.Rodriguez Castillo, José del CarmenSanchez Prieto, L.Casao, A.OvinosRazas (animales)Conservación de EmbrionesExperimentación in VitroExperimentación in VivoSuperovulaciónSheepBreeds (animals)Embryo PreservationIn Vitro ExperimentationIn Vivo ExperimentationSuperovulationThis study investigated the use of previously superovulated ovaries as a source of oocytes, assessing the competence of them for in vitro embryo production. Two superovulatory treatments were performed: equine Chorionic Gonadotrophin (eCG) plus porcine Follicle-Stimulating Hormone (pFSH) in a single dose or the conventional protocol of six decreasing doses of pFSH. Thirty donor ewes of the endangered Ojalada breed were given either the simplified (group S; n=15) or the decreasing-dose (group D; n=15) treatments three times at intervals of ≥50 days. Ovaries were recovered on day 7 after the oestrus following the third treatment, just after embryo flushing, and the oocytes were collected to assess in vitro maturation, fertilisation and development to the blastocyst stage. The two superovulatory treatments did not differ in the mean number of oocytes selected for maturation (7.1±1.2 and 8.5±1.5 per ewe in the D and S groups, respectively). The oocytes recovered from ewes in Group D (87.5%) had a significantly (p<0.05) higher maturation rate than did those recovered from ewes in group S (75%), but no differences were found in fertilisation rate (94% and 94.6% in the D and S groups, respectively); both groups did not differ in their blastocyst rates and the total number of cells in in vitro-produced blastocysts. In the two experimental groups, 1.7 (D) and 1.8 (S) in vitro-produced blastocysts were generated per ewe, which indicate that it is feasible to combine in vivo and in vitro techniques to maximise embryo production in endangered sheep breeds.EEA ChubutFil: Forcada, Fernando. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; EspañaFil: Buffoni, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chubut; ArgentinaFil: Abecia, José Alfonso. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; EspañaFil: Asenjo, B. Universidad de Valladolid. Escuela Universitaria de Ingenierías Agrarias de Soria; EspañaFil: Palacin, José Ignacio. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; EspañaFil: Vázquez, M.I. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; EspañaFil: Rodriguez Castillo, José del Carmen. Benemérita Universidad Autónoma de Puebla. Facultad de Medicina Veterinaria y Zootecnia; MéxicoFil: Sanchez Prieto, L. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; EspañaFil: Casao, A. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; EspañaTaylor & Francis2019-05-08T14:17:03Z2019-05-08T14:17:03Z2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.tandfonline.com/doi/full/10.1080/09712119.2011.622929http://hdl.handle.net/20.500.12123/50730971-21190974-1844https://doi.org/10.1080/09712119.2011.622929Journal of Applied Animal Research 39 (4) : 412-417 (2011)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:57Zoai:localhost:20.500.12123/5073instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:58.217INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques
title Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques
spellingShingle Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques
Forcada, Fernando
Ovinos
Razas (animales)
Conservación de Embriones
Experimentación in Vitro
Experimentación in Vivo
Superovulación
Sheep
Breeds (animals)
Embryo Preservation
In Vitro Experimentation
In Vivo Experimentation
Superovulation
title_short Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques
title_full Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques
title_fullStr Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques
title_full_unstemmed Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques
title_sort Maximising embryo production in endangered sheep breeds: in vitro procedures that complement in vivo techniques
dc.creator.none.fl_str_mv Forcada, Fernando
Buffoni, Andres
Abecia, José Alfonso
Asenjo, B.
Palacin, José Ignacio
Vázquez, M.I.
Rodriguez Castillo, José del Carmen
Sanchez Prieto, L.
Casao, A.
author Forcada, Fernando
author_facet Forcada, Fernando
Buffoni, Andres
Abecia, José Alfonso
Asenjo, B.
Palacin, José Ignacio
Vázquez, M.I.
Rodriguez Castillo, José del Carmen
Sanchez Prieto, L.
Casao, A.
author_role author
author2 Buffoni, Andres
Abecia, José Alfonso
Asenjo, B.
Palacin, José Ignacio
Vázquez, M.I.
Rodriguez Castillo, José del Carmen
Sanchez Prieto, L.
Casao, A.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ovinos
Razas (animales)
Conservación de Embriones
Experimentación in Vitro
Experimentación in Vivo
Superovulación
Sheep
Breeds (animals)
Embryo Preservation
In Vitro Experimentation
In Vivo Experimentation
Superovulation
topic Ovinos
Razas (animales)
Conservación de Embriones
Experimentación in Vitro
Experimentación in Vivo
Superovulación
Sheep
Breeds (animals)
Embryo Preservation
In Vitro Experimentation
In Vivo Experimentation
Superovulation
dc.description.none.fl_txt_mv This study investigated the use of previously superovulated ovaries as a source of oocytes, assessing the competence of them for in vitro embryo production. Two superovulatory treatments were performed: equine Chorionic Gonadotrophin (eCG) plus porcine Follicle-Stimulating Hormone (pFSH) in a single dose or the conventional protocol of six decreasing doses of pFSH. Thirty donor ewes of the endangered Ojalada breed were given either the simplified (group S; n=15) or the decreasing-dose (group D; n=15) treatments three times at intervals of ≥50 days. Ovaries were recovered on day 7 after the oestrus following the third treatment, just after embryo flushing, and the oocytes were collected to assess in vitro maturation, fertilisation and development to the blastocyst stage. The two superovulatory treatments did not differ in the mean number of oocytes selected for maturation (7.1±1.2 and 8.5±1.5 per ewe in the D and S groups, respectively). The oocytes recovered from ewes in Group D (87.5%) had a significantly (p<0.05) higher maturation rate than did those recovered from ewes in group S (75%), but no differences were found in fertilisation rate (94% and 94.6% in the D and S groups, respectively); both groups did not differ in their blastocyst rates and the total number of cells in in vitro-produced blastocysts. In the two experimental groups, 1.7 (D) and 1.8 (S) in vitro-produced blastocysts were generated per ewe, which indicate that it is feasible to combine in vivo and in vitro techniques to maximise embryo production in endangered sheep breeds.
EEA Chubut
Fil: Forcada, Fernando. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España
Fil: Buffoni, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chubut; Argentina
Fil: Abecia, José Alfonso. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España
Fil: Asenjo, B. Universidad de Valladolid. Escuela Universitaria de Ingenierías Agrarias de Soria; España
Fil: Palacin, José Ignacio. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España
Fil: Vázquez, M.I. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España
Fil: Rodriguez Castillo, José del Carmen. Benemérita Universidad Autónoma de Puebla. Facultad de Medicina Veterinaria y Zootecnia; México
Fil: Sanchez Prieto, L. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España
Fil: Casao, A. Universidad de Zaragoza. Instituto de Investigación de Ciencias Ambientales de Aragón. Grupo de Biología y Fisiología de la Reproducción; España
description This study investigated the use of previously superovulated ovaries as a source of oocytes, assessing the competence of them for in vitro embryo production. Two superovulatory treatments were performed: equine Chorionic Gonadotrophin (eCG) plus porcine Follicle-Stimulating Hormone (pFSH) in a single dose or the conventional protocol of six decreasing doses of pFSH. Thirty donor ewes of the endangered Ojalada breed were given either the simplified (group S; n=15) or the decreasing-dose (group D; n=15) treatments three times at intervals of ≥50 days. Ovaries were recovered on day 7 after the oestrus following the third treatment, just after embryo flushing, and the oocytes were collected to assess in vitro maturation, fertilisation and development to the blastocyst stage. The two superovulatory treatments did not differ in the mean number of oocytes selected for maturation (7.1±1.2 and 8.5±1.5 per ewe in the D and S groups, respectively). The oocytes recovered from ewes in Group D (87.5%) had a significantly (p<0.05) higher maturation rate than did those recovered from ewes in group S (75%), but no differences were found in fertilisation rate (94% and 94.6% in the D and S groups, respectively); both groups did not differ in their blastocyst rates and the total number of cells in in vitro-produced blastocysts. In the two experimental groups, 1.7 (D) and 1.8 (S) in vitro-produced blastocysts were generated per ewe, which indicate that it is feasible to combine in vivo and in vitro techniques to maximise embryo production in endangered sheep breeds.
publishDate 2011
dc.date.none.fl_str_mv 2011
2019-05-08T14:17:03Z
2019-05-08T14:17:03Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.tandfonline.com/doi/full/10.1080/09712119.2011.622929
http://hdl.handle.net/20.500.12123/5073
0971-2119
0974-1844
https://doi.org/10.1080/09712119.2011.622929
url https://www.tandfonline.com/doi/full/10.1080/09712119.2011.622929
http://hdl.handle.net/20.500.12123/5073
https://doi.org/10.1080/09712119.2011.622929
identifier_str_mv 0971-2119
0974-1844
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv Journal of Applied Animal Research 39 (4) : 412-417 (2011)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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